Review




Structured Review

Gilupi GmbH release buffer 1x
Release Buffer 1x, supplied by Gilupi GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/release+buffer/release+buffer+1x/pmc05324062-83-49-52
Average 90 stars, based on 1 article reviews
release buffer 1x - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Incubation:

Article Title: Catch and Release: rare cell analysis from a functionalised medical wire
Article Snippet: The CellCollectors were charged with target cells by incubation with 5 ml spiked cell suspension at room temperature (RT) in a 9 ml reaction tube (Venosafe) on a horizontal roller mixer (SRT6D, Stuart Scientific) at 5 rpm for 30 min. For cell detachment, the C&R was incubated in pre-warmed 1x release buffer (GILUPI) at 37 °C for 10 min followed by agitation at 200 rpm on a plate shaker (DELFIA, Perkin-Elmer) at RT for 15 min. After centrifugation at 300× g (Heraeus Megafuge 40R, Thermo Fisher) for 10 min we gently removed the C&R device and centrifuged again.

Suspension:

Article Title: Catch and Release: rare cell analysis from a functionalised medical wire
Article Snippet: The CellCollectors were charged with target cells by incubation with 5 ml spiked cell suspension at room temperature (RT) in a 9 ml reaction tube (Venosafe) on a horizontal roller mixer (SRT6D, Stuart Scientific) at 5 rpm for 30 min. For cell detachment, the C&R was incubated in pre-warmed 1x release buffer (GILUPI) at 37 °C for 10 min followed by agitation at 200 rpm on a plate shaker (DELFIA, Perkin-Elmer) at RT for 15 min. After centrifugation at 300× g (Heraeus Megafuge 40R, Thermo Fisher) for 10 min we gently removed the C&R device and centrifuged again.

Centrifugation:

Article Title: Catch and Release: rare cell analysis from a functionalised medical wire
Article Snippet: The CellCollectors were charged with target cells by incubation with 5 ml spiked cell suspension at room temperature (RT) in a 9 ml reaction tube (Venosafe) on a horizontal roller mixer (SRT6D, Stuart Scientific) at 5 rpm for 30 min. For cell detachment, the C&R was incubated in pre-warmed 1x release buffer (GILUPI) at 37 °C for 10 min followed by agitation at 200 rpm on a plate shaker (DELFIA, Perkin-Elmer) at RT for 15 min. After centrifugation at 300× g (Heraeus Megafuge 40R, Thermo Fisher) for 10 min we gently removed the C&R device and centrifuged again.



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