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Immunotec inc histamine-release buffer
Histamine Release Buffer, supplied by Immunotec inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/release+buffer/histamine+release+buffer/pm09065447-174-23-25
Average 90 stars, based on 1 article reviews
histamine-release buffer - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

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Article Title: BTK inhibition is a potent approach to block IgE‐mediated histamine release in human basophils
Article Snippet: Histamine release buffer (HRB) and histamine radioimmunoassay (RIA) kit were purchased from Immunotech, and RPMI 1640 medium, Iscove's modified Dulbecco's medium (IMDM), and fetal calf serum (FCS) from Thermo Fisher Scientific (Waltham, MA, USA).

Article Title: The PI3-Kinase/mTOR-Targeting Drug NVP-BEZ235 Inhibits Growth and IgE-Dependent Activation of Human Mast Cells and Basophils
Article Snippet: Histamine release buffer (HRB), anti-IgE antibody E-124.2.8, and a histamine radioimmunoassay (RIA) were purchased from Immunotech (Marseille, France).

Incubation:

Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis.
Article Snippet: .. Dextrannriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) ere incubated in histamine release buffer (Immunotech, Marseille, France) n the absence or presence of polyclonal anti-IgE antibody (0.01 −10 μg/ml) t 37 °C for 2 h. After incubation, cells were centrifuged at 4 °C, and the ell-free supernatants and total suspensions were analyzed for histamine ontent by radioimmunoassay (Immunotech). ..

Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis
Article Snippet: .. Dextran-enriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) were incubated in histamine release buffer (Immunotech, Marseille, France) in the absence or presence of polyclonal anti-IgE antibody (0.01−10 μg/ml) at 37°C for 2 h. After incubation, cells were centrifuged at 4°C, and the cell-free supernatants and total suspensions were analyzed for histamine content by radioimmunoassay (Immunotech). ..

Article Title: Effects of ibrutinib on proliferation and histamine release in canine neoplastic mast cells.
Article Snippet: .. After incubation, cells were washed in phosphate-buffered saline (PBS, Gibco Life Technologies), resuspended in histamine release buffer (HRB, Immunotech, Marseille, France) and then incubated with 5 μg/mL goat anti-dog-IgE antibody (Bethyl Laboratories) at 37 C for 30 minutes. .. After incubation, cells were centrifuged at 4 C, and the cell-free supernatants and cell pellets were recovered and analysed for histamine content by radioimmunoassay (RIA, Immunotech).

RIA Assay:

Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis.
Article Snippet: .. Dextrannriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) ere incubated in histamine release buffer (Immunotech, Marseille, France) n the absence or presence of polyclonal anti-IgE antibody (0.01 −10 μg/ml) t 37 °C for 2 h. After incubation, cells were centrifuged at 4 °C, and the ell-free supernatants and total suspensions were analyzed for histamine ontent by radioimmunoassay (Immunotech). ..

Article Title: Secondary basophilic leukemia in Ph-negative myeloid neoplasms: A distinct subset with poor prognosis
Article Snippet: .. Dextran-enriched basophils (purity: #1: 45%; #2; not available; #3: 23%; #4: 3%) were incubated in histamine release buffer (Immunotech, Marseille, France) in the absence or presence of polyclonal anti-IgE antibody (0.01−10 μg/ml) at 37°C for 2 h. After incubation, cells were centrifuged at 4°C, and the cell-free supernatants and total suspensions were analyzed for histamine content by radioimmunoassay (Immunotech). ..

Sedimentation:

Article Title: Gastro-duodenal digestion products of the major peanut allergen Ara h 1 retain an allergenic potential.
Article Snippet: Gastro-duodenal digestion products of the major peanut allergen Ara h 1 retain an allergenic potential T. Eiwegger , N. Rigby, L. Mondouletz, H. Bernardz, M.-T. Krauth, A. Boehm, E. Dehlink , P. Valent, J. M. Walz, E. N. C. Mills and Z. Szépfalusi Department of Pediatrics, Division of General Pediatrics, Medical University of Vienna, Vienna, Austria, Institute of Food Research, Norwich Research Park, Norwich, UK, zLaboratoire d’Immuno-Allergie Alimentaire, INRA-CEA Saclay, France and Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria

Saline:

Article Title: Effects of ibrutinib on proliferation and histamine release in canine neoplastic mast cells.
Article Snippet: .. After incubation, cells were washed in phosphate-buffered saline (PBS, Gibco Life Technologies), resuspended in histamine release buffer (HRB, Immunotech, Marseille, France) and then incubated with 5 μg/mL goat anti-dog-IgE antibody (Bethyl Laboratories) at 37 C for 30 minutes. .. After incubation, cells were centrifuged at 4 C, and the cell-free supernatants and cell pellets were recovered and analysed for histamine content by radioimmunoassay (RIA, Immunotech).



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