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c glutamicum atcc 13032 reference genome  (ATCC)


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    Structured Review

    ATCC c glutamicum atcc 13032 reference genome
    Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).
    C Glutamicum Atcc 13032 Reference Genome, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 3143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/reference+atcc/Corynebacterium+Glutamicum%3B+534/pmc12914118-204-21-23
    Average 99 stars, based on 3143 article reviews
    c glutamicum atcc 13032 reference genome - by Bioz Stars, 2026-11
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    1) Product Images from "Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress"

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress

    Journal: Synthetic and Systems Biotechnology

    doi: 10.1016/j.synbio.2026.01.020

    Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).
    Figure Legend Snippet: Improving the tolerance to formaldehyde via ALE. (A) Growth of FM-1 in minimal medium with 10 g/L glucose and different formaldehyde concentrations. 0 mM (square), 0.5 mM (triangle), 0.8 mM (circle), and 1 mM (inverted triangle). (B) ALE procedure of culture-1 in CGXII minimal medium supplemented with different formaldehyde concentrations and 10 g/L glucose. (C) Growth curve of the evolved mutants in CGXII minimal medium supplemented with 10 g/L glucose and 0.8 mM formaldehyde. (D) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose and 1.6 mM formaldehyde. (E) Growth curve of evolved mutant in CGXII minimal medium supplemented with 10 g/L glucose. (F) Formaldehyde degradation during cell growth of wild-type C. glutamicum ATCC 13032, FM-1 and FM-3. Values and error bars reflect the mean ± s.d. of three biological replicates (N = 3).

    Techniques Used: Mutagenesis

    Related Articles

    Mutagenesis:

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The pCas9gRNA- cgl0752 and the ssDNA containing cgl0752 G761T mutation were co-transformed into C. glutamicum ATCC 13032 (pRecT) via electroporation. ..

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The mutant cgl1590 fragment was amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primer pairs cgl1590 750insG -F1/ cgl1590 750insG - R1, cgl1590 750insG -F2/ cgl1590 750insG -R2, and then ligated with the Bam HI-linearized pK18 mobsacB using the ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech, China). .. Primer synthesis and Sanger sequencing were performed by AZENTA (China).

    Electroporation:

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The pCas9gRNA- cgl0752 and the ssDNA containing cgl0752 G761T mutation were co-transformed into C. glutamicum ATCC 13032 (pRecT) via electroporation. ..

    Expressing:

    Article Title: Production of Gamma-Aminobutyric Acid by Levilactobacillus brevis AK19B: Strain Characterization and Process Optimization Using Box-Behnken Design.
    Article Snippet: .. Shi F, Li Y (2011) Synthesis of γ-aminobutyric acid by expressing Lactobacillus brevis-derived glutamate decarboxylase in the Corynebacterium glutamicum strain ATCC 13032. ..

    other:

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: C. glutamicum ATCC 13032 possesses an endogenous formaldehyde dissimilation pathway.

    Amplification:

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The mutant cgl1590 fragment was amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primer pairs cgl1590 750insG -F1/ cgl1590 750insG - R1, cgl1590 750insG -F2/ cgl1590 750insG -R2, and then ligated with the Bam HI-linearized pK18 mobsacB using the ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech, China). .. Primer synthesis and Sanger sequencing were performed by AZENTA (China).

    Cloning:

    Article Title: Multi-omics analyses of evolved Corynebacterium glutamicum mutants reveal the molecular responses to formaldehyde stress
    Article Snippet: .. The mutant cgl1590 fragment was amplified from the genomic DNA of C. glutamicum ATCC 13032 using the primer pairs cgl1590 750insG -F1/ cgl1590 750insG - R1, cgl1590 750insG -F2/ cgl1590 750insG -R2, and then ligated with the Bam HI-linearized pK18 mobsacB using the ClonExpress MultiS One Step Cloning Kit (Vazyme Biotech, China). .. Primer synthesis and Sanger sequencing were performed by AZENTA (China).



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