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PrimerDesign Inc precision qscriptt reverse transcription kit
Precision Qscriptt Reverse Transcription Kit, supplied by PrimerDesign Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/qscript+kit/precision+qscript+reverse+transcription+kit/pmc06291066-104-48-53
Average 90 stars, based on 1 article reviews
precision qscriptt reverse transcription kit - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Synthesized:

Article Title: Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats.
Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from 1 mg of RNA using the Precision qScript Reverse Transcription kit (Primer Design) and oligo-dT primers (Table 1). ..

Article Title: Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from 1 mg of RNA using the Precision qScript Reverse Transcription kit (Primer Design) and oligo-dT primers ( ). ..

Reverse Transcription:

Article Title: Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats.
Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from 1 mg of RNA using the Precision qScript Reverse Transcription kit (Primer Design) and oligo-dT primers (Table 1). ..

Article Title: Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats
Article Snippet: .. Following the manufacturer’s instructions, cDNA was synthesized from 1 mg of RNA using the Precision qScript Reverse Transcription kit (Primer Design) and oligo-dT primers ( ). ..

Article Title: Sonic Hedgehog Signaling Promotes Peri-Lesion Cell Proliferation and Functional Improvement after Cortical Contusion Injury
Article Snippet: Tissue was transferred in ice-cold artificial CSF (aCSF) and placed directly into TRIzol (Invitrogen). .. Total RNA was extracted and directly reverse-transcribed to complementary DNA (cDNA) using a Precision qScript RT Kit (Primer Design). .. The cDNA was amplified using a One-Step PCR Kit (Primer Design) in a real-time thermocycler (Rotor-Gene 6000, Corbett Robotics).

Article Title: Plasma GBP2 promoter methylation is associated with advanced stages in breast cancer
Article Snippet: .. Two micrograms of total RNA was digested by 2 μg DNase 1 (Fermentas, Manchester, UK) to remove genomic DNA contamination and then 1 μg of RNA was used for cDNA synthesis, with Precision qScript T Reverse Transcription Kit (Primerdesign, Chandlers’s Ford, UK). ..

Article Title: Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer
Article Snippet: Mature miR expression was quantified by quantitative real-time RT-PCR using TaqMan microRNA assays and TaqMan Universal PCR Master Mix (Applied Biosystems) according to the manufacturer’s protocol. .. For detection of mRNAs, cDNA was prepared from 500 ng total RNA using Precision qScript Reverse Transcription kit (PrimerDesign) and oligo d(T) primers. cDNAs were amplified using 2× Fast SYBR Green Master Mix (Applied Biosystems) and 250 nM forward and reverse primers (Table ). ..

Article Title: Zebrafish skeletal muscle cell cultures: Monolayer to three-dimensional tissue engineered collagen constructs
Article Snippet: Cells in monolayer cultures were scraped and homogenised, whereas collagen constructs were homogenised using a hand-held stick homogeniser (IKA T10 Fisher Scientific, UK) after being washed several times in PBS. .. RNA (1 μg) was transcribed using the Precision mqScript Reverse Transcription Kit (Primerdesign Ltd, UK) according to the manufacturer’s protocol and the cDNA was diluted 1:2 in molecular biology grade water (Sigma,UK). .. Gene expression analysis was performed by quantitative real time PCR on a RotorGene 6000 cycler (Corbett Research, UK) using a 72 well rotor.

Article Title: Systemic Exosomal Delivery of shRNA Minicircles Prevents Parkinsonian Pathology
Article Snippet: Total RNA was isolated using the RNeasy Kit (QIAGEN) as per the manufacturer’s protocol. .. Reverse transcription (RT) was performed with the qSCRIP Reverse Transcriptase Kit (Primer Design, Southampton, England) as per the manufacturer’s instructions. qPCR experiments were performed on the StepOne Real-Time PCR System (Applied Biosystems) using Precision qPCR Mastermix (Applied Biosystems). ..

cDNA Synthesis:

Article Title: Plasma GBP2 promoter methylation is associated with advanced stages in breast cancer
Article Snippet: .. Two micrograms of total RNA was digested by 2 μg DNase 1 (Fermentas, Manchester, UK) to remove genomic DNA contamination and then 1 μg of RNA was used for cDNA synthesis, with Precision qScript T Reverse Transcription Kit (Primerdesign, Chandlers’s Ford, UK). ..

Amplification:

Article Title: Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer
Article Snippet: Mature miR expression was quantified by quantitative real-time RT-PCR using TaqMan microRNA assays and TaqMan Universal PCR Master Mix (Applied Biosystems) according to the manufacturer’s protocol. .. For detection of mRNAs, cDNA was prepared from 500 ng total RNA using Precision qScript Reverse Transcription kit (PrimerDesign) and oligo d(T) primers. cDNAs were amplified using 2× Fast SYBR Green Master Mix (Applied Biosystems) and 250 nM forward and reverse primers (Table ). ..

SYBR Green Assay:

Article Title: Androgen receptor-modulatory microRNAs provide insight into therapy resistance and therapeutic targets in advanced prostate cancer
Article Snippet: Mature miR expression was quantified by quantitative real-time RT-PCR using TaqMan microRNA assays and TaqMan Universal PCR Master Mix (Applied Biosystems) according to the manufacturer’s protocol. .. For detection of mRNAs, cDNA was prepared from 500 ng total RNA using Precision qScript Reverse Transcription kit (PrimerDesign) and oligo d(T) primers. cDNAs were amplified using 2× Fast SYBR Green Master Mix (Applied Biosystems) and 250 nM forward and reverse primers (Table ). ..

other:

Article Title: Androgen receptor modulatory miR-1271-5p can promote hormone sensitive prostate cancer cell growth
Article Snippet: cDNA was prepared from 500ng total RNA using Precision qScript Reverse Tran-scription kit (PrimerDesign, UK) and oligo d(T) primers. cDNA samples were amplified using 2x Fast SYBR Green Master Mix (SYBR Green I Dye, AmpliTaq Fast DNA Polymerase, Uracil- DNA Glycosylase, ROX dye Passive Reference, dNTPs and optimised buffer components) (Applied Biosystems, MA, USA) and 250nM forward and reverse primers ( ).

Real-time Polymerase Chain Reaction:

Article Title: Systemic Exosomal Delivery of shRNA Minicircles Prevents Parkinsonian Pathology
Article Snippet: Total RNA was isolated using the RNeasy Kit (QIAGEN) as per the manufacturer’s protocol. .. Reverse transcription (RT) was performed with the qSCRIP Reverse Transcriptase Kit (Primer Design, Southampton, England) as per the manufacturer’s instructions. qPCR experiments were performed on the StepOne Real-Time PCR System (Applied Biosystems) using Precision qPCR Mastermix (Applied Biosystems). ..



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