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the protocol for the negative retention of the cd4+cd25- cell population  (Miltenyi Biotec)


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    Miltenyi Biotec the protocol for the negative retention of the cd4+cd25- cell population
    The Protocol For The Negative Retention Of The Cd4+Cd25 Cell Population, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/protocol+cd4/cd14/pmc03935776-175-8-14
    Average 90 stars, based on 1 article reviews
    the protocol for the negative retention of the cd4+cd25- cell population - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Retro-prospective Observational Study on the Role of the β3 Adrenergic Receptor as a Tumour Biomarker in Paediatric Solid Tumours
    Article Snippet: Data will be acquired at the MacsQuant Miltenyi Biotech cytofluorometer and analysed withFlowLogic® software programme.. Time Frame: through study completion, an average of 1 year

    Article Title: Allogeneic Hematopoietic Stem Cell Transplantation for Severe Aplastic Anemia and Other Bone Marrow Failure Syndromes Using G-CSF Mobilized CD34+ Selected Hematopoietic Precursor Cells Co-Infused With a Reduced Dose of Non-Mobilized Donor T-Cells
    Article Snippet: Using the Miltenyi CliniMACs system, recipients will receive an allograft on day 0 containing donor CD34+ cells that have been positively selected and T-cell depleted following G-CSF mobilization (goal CD34+ cell dose of 5 times 10(6) CD34+ cells /kg recipient) combined with 2 times 10(7) cells/kg of non-mobilized CD3+ T-cells previously collected and cryopreserved from the same donor by apheresis prior to G-CSF mobilization.

    Article Title: Phase 1 Study of Autologous Tris-CAR-T Cell Locoregional Immunotherapy for Recurrent Glioblastoma
    Article Snippet: Autologous Tris-CAR-T cell will be manufactured via CliniMACS Instrument (Miltenyi Biotec).

    Article Title: BT13BTθ51 Unrelated and Partially Matched Related Donor Peripheral Stem Cell Transplantation With Alpha Beta T Cell and B Cell Depletion For Patients With Hematologic Malignancies
    Article Snippet: The purpose of this research study is to evaluate a new method of T cell depletion using the Miltenyi CliniMACSTM device for patients undergoing a peripheral stem cell transplant utilizing either a unrelated donor or partially matched/haploidentical related donor.

    Selection:

    Article Title: T-Cell Receptor Alpha Beta+/CD19+ Depletion in Haploidentical Allogeneic Hematopoietic Cell Transplantation (Allo-HCT) for Adult and Pediatric Patients With Hematological Malignancies and Non-malignant Disorders
    Article Snippet: .. The peripheral blood hematopoietic cell product will undergo negative selection of TCR αβ following the standardized protocol in the user's manual for the CliniMACS (Miltenyi Biotech, Germany). ..

    Isolation:

    Article Title: EGR2 is an epigenomic regulator of phagocytosis and antifungal immunity in alveolar macrophages
    Article Snippet: .. The AMs were isolated by BAL and were purified with CD45 magnetic beads (Miltenyi Biotec). ..

    Purification:

    Article Title: EGR2 is an epigenomic regulator of phagocytosis and antifungal immunity in alveolar macrophages
    Article Snippet: .. The AMs were isolated by BAL and were purified with CD45 magnetic beads (Miltenyi Biotec). ..

    Magnetic Beads:

    Article Title: EGR2 is an epigenomic regulator of phagocytosis and antifungal immunity in alveolar macrophages
    Article Snippet: .. The AMs were isolated by BAL and were purified with CD45 magnetic beads (Miltenyi Biotec). ..

    Single Cell:

    Article Title: CD300f immune receptor is a microglial tissue damage sensor and regulates efferocytosis after brain damage
    Article Snippet: .. Three to five brain punches of 2 mm 2 per group were pooled together, and microglial cells were then dissociated into a single-cell suspension using the Miltenyi Tumor Dissociation Kit (130-096-730), GentleMACS 25C Tubes (130-093-237), and GentleMACS Octo Dissociator. .. The cells were isolated using anti-human CD11b beads (Miltenyi 130-093-636) and myelin removal beads (Miltenyi 130-096-733).

    Suspension:

    Article Title: CD300f immune receptor is a microglial tissue damage sensor and regulates efferocytosis after brain damage
    Article Snippet: .. Three to five brain punches of 2 mm 2 per group were pooled together, and microglial cells were then dissociated into a single-cell suspension using the Miltenyi Tumor Dissociation Kit (130-096-730), GentleMACS 25C Tubes (130-093-237), and GentleMACS Octo Dissociator. .. The cells were isolated using anti-human CD11b beads (Miltenyi 130-093-636) and myelin removal beads (Miltenyi 130-096-733).



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    (A) Conventional T cells develop normally in the absence of p53. Upper panel, <t>CD4</t> and CD8 profile of total thymocytes. Lower panel, CD4 and CD8 profile of TCRβ high matured thymocytes. (B) Representative gating strategy for thymic NKT1 , NKT2 and NKT17 subsets. (C) p53 protein expression levels were analyzed by flow cytometry at steady state and after irradiation. For irradiation condition, cells were irradiated (1,000 rad) and incubated for 4 hours at 37°C. Data is pooled result of two independent experiments. (D) γH2AX expression in thymic iNKT subset was analyzed by flow cytometry. Data are mean ± SEM. Statistical differences between groups were analyzed with One-way ANOVA followed by multiple comparison. * * p < 0.005, * * * p < 0.0001.
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    (A) Conventional T cells develop normally in the absence of p53. Upper panel, CD4 and CD8 profile of total thymocytes. Lower panel, CD4 and CD8 profile of TCRβ high matured thymocytes. (B) Representative gating strategy for thymic NKT1 , NKT2 and NKT17 subsets. (C) p53 protein expression levels were analyzed by flow cytometry at steady state and after irradiation. For irradiation condition, cells were irradiated (1,000 rad) and incubated for 4 hours at 37°C. Data is pooled result of two independent experiments. (D) γH2AX expression in thymic iNKT subset was analyzed by flow cytometry. Data are mean ± SEM. Statistical differences between groups were analyzed with One-way ANOVA followed by multiple comparison. * * p < 0.005, * * * p < 0.0001.

    Journal: bioRxiv

    Article Title: Tumor suppressor p53 controls thymic NKT17 development

    doi: 10.1101/2024.08.21.608967

    Figure Lengend Snippet: (A) Conventional T cells develop normally in the absence of p53. Upper panel, CD4 and CD8 profile of total thymocytes. Lower panel, CD4 and CD8 profile of TCRβ high matured thymocytes. (B) Representative gating strategy for thymic NKT1 , NKT2 and NKT17 subsets. (C) p53 protein expression levels were analyzed by flow cytometry at steady state and after irradiation. For irradiation condition, cells were irradiated (1,000 rad) and incubated for 4 hours at 37°C. Data is pooled result of two independent experiments. (D) γH2AX expression in thymic iNKT subset was analyzed by flow cytometry. Data are mean ± SEM. Statistical differences between groups were analyzed with One-way ANOVA followed by multiple comparison. * * p < 0.005, * * * p < 0.0001.

    Article Snippet: Anti-CD4 (RM4-5)-BV510 (Need clone name or described it in Star Protocol), anti-B220 (RA3-6B2)-AF700, anti-CD44 (IM7)-FITC, anti-TCRβ (H57-597)-PE/Cy7, anti-RORγt (Q31-378)-BV786, anti-T-bet (O4-46)-BV421, anti-PLZF (R17-809)-AF647, anti-CCR7 (4B12)-BV786, anti-IL-17A (TC11-18H10)-FITC, anti-IFNγ (XMG1.2)-FITC, anti-IL-4 (11B11)-BV421 were purchased from BD Biosciences.

    Techniques: Expressing, Flow Cytometry, Irradiation, Incubation, Comparison

    (A) Ki67 expression in thymic iNKT subset was analyzed by flow cytometry. Data is pooled result of two independent experiments. WT, n=2; cKO, n=2. (B) γH2AX expression in thymic iNKT subset was analyzed by flow cytometry. Data is pooled result of three independent experiments. SP4; CD4 single positive thymocytes. WT, n=3; cKO, n=4. Data are mean ± SEM. Statistical differences were analyzed with student t-test. * p < 0.05.

    Journal: bioRxiv

    Article Title: Tumor suppressor p53 controls thymic NKT17 development

    doi: 10.1101/2024.08.21.608967

    Figure Lengend Snippet: (A) Ki67 expression in thymic iNKT subset was analyzed by flow cytometry. Data is pooled result of two independent experiments. WT, n=2; cKO, n=2. (B) γH2AX expression in thymic iNKT subset was analyzed by flow cytometry. Data is pooled result of three independent experiments. SP4; CD4 single positive thymocytes. WT, n=3; cKO, n=4. Data are mean ± SEM. Statistical differences were analyzed with student t-test. * p < 0.05.

    Article Snippet: Anti-CD4 (RM4-5)-BV510 (Need clone name or described it in Star Protocol), anti-B220 (RA3-6B2)-AF700, anti-CD44 (IM7)-FITC, anti-TCRβ (H57-597)-PE/Cy7, anti-RORγt (Q31-378)-BV786, anti-T-bet (O4-46)-BV421, anti-PLZF (R17-809)-AF647, anti-CCR7 (4B12)-BV786, anti-IL-17A (TC11-18H10)-FITC, anti-IFNγ (XMG1.2)-FITC, anti-IL-4 (11B11)-BV421 were purchased from BD Biosciences.

    Techniques: Expressing, Flow Cytometry

    Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from 2D2 mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.

    Journal: Acta biomaterialia

    Article Title: Intrinsic immunogenicity of rapidly-degradable polymers evolves during degradation

    doi: 10.1016/j.actbio.2015.12.026

    Figure Lengend Snippet: Poly1 particles increase MHCI-mediated antigen presentation and antigen-specific T cell proliferation. (A) MFI of signal pertaining to SIINFEKL presented in MHCI following treatment of DCs with no SIINFEKL (“Untreated”, gray), buffer with SIINFEKL (“SIIN Only”, gray), free Poly1 with SIINFEKL (blue), or Poly1 particles with SIINFEKL (red) and gated under DAPI− cells. 48 hours after treatment of primary DCs as in (A) but with 0.25ug mL−1 of a different model antigen, MOG35-55,CD4+ cells isolated from 2D2 mice with T cell receptors specific to MOG35-55 were added to the wells and incubated for 72 hours. (B) Representative flow cytometry traces showing CFSE dilutions after treatment with MOG35-55 and either buffer (“MOG Only”, gray), TLR4a LPS (“TLR4”, black), free Poly1 (“Free”, blue), and Poly1 particles formed (“Particles”, red). (C) MFI of CFSE signal among DAPI−/CD3+/CD4+ cells following treatments as in (B). (D) Percentage of DAPI−/CD3+/CD4+ cells proliferated beyond the 2nd generation following treatment as in (B) as determined by CFSE dilution. Samples were prepared in triplicated and errors bars represent SEM.

    Article Snippet: After 48 hours in culture, CD4 + T cells were isolated from the spleens of 2D2 mice using the manufacturer’s recommended protocol (STEMCELL).

    Techniques: Immunopeptidomics, Isolation, Incubation, Flow Cytometry