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ProMat Inc protein microarray analysis tool (promat)
Protein Microarray Analysis Tool (Promat), supplied by ProMat Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/protein+microarray/protein+microarray+analysis+tool++promat+/pmc03132300-75-16-20
Average 90 stars, based on 1 article reviews
protein microarray analysis tool (promat) - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Microarray:

Article Title: Protein secretion in human mammary epithelial cells following HER1 receptor activation: influence of HER2 and HER3 expression
Article Snippet: .. Spot fluorescent data were then processed and analyzed using Protein Microarray Analysis Tool (ProMAT) and ProMAT Calibrator [ - ], both of which are open-source, freeware programs (available at http://www.pnl.gov/statistics/ProMAT/ ) that we developed specifically for processing ELISA microarray data. ..

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.
Article Snippet: Fluorescence data were quantied using ScanArray Express soware (Perkin-Elmer). .. Data analysis was performed using ProMAT Calibrator and the Protein Microarray Analysis Tool (ProMAT).58,59 This publicly available soware normalizes data to a control protein, then ts the data to standard curves and ffer, milk and plasma using an ELISA protein microarray. ..

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Data, Statistics and ROC analyses Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Susceptibility to quantum dot induced lung inflammation differs widely among the Collaborative Cross founder mouse strains
Article Snippet: .. Standard curves were then fit with the Protein Microarray Analysis Tool (ProMAT) using the four-parameter logistic curve-fitting option ( White et al., 2006 ) and used by ProMAT to generate antigen concentration values for the samples based on the adjusted spot fluorescent intensities. ..

Article Title: Macrophage Responses to Silica Nanoparticles are Highly Conserved Across Particle Sizes
Article Snippet: The fluorescent images for the slides were obtained using ScanArray ExpressHT laser scanner and the spot intensity quantified using ScanArray Express (Perkin Elmer, Wellesley, MA). .. Standard curves were generated and sample antigen concentrations were calculated using the Protein Microarray Analysis Tool (ProMAT), which is a custom freeware program that we developed specifically for this use ( White et al. , 2006 ). . Gene expression and pathway analysis. .. Whole genome microarray analysis was performed using Affymetrix Mouse Genome 430A 2.0 chips (Affymetrix, Santa Clara, CA; 22,690 probesets).

Article Title: Antibody microarrays for high-throughput, multianalyte analysis
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) microarray technology promises to be a powerful tool for detecting and validating protein biomarkers, especially panels of biomarkers.. ELISA microarrays are capable of high-throughput analysis of multiple proteins using small sample volumes.. In this chapter we review the literature on the use of antibody microarrays for biomarker discovery and validation.

Article Title: Diet-induced obesity reprograms the inflammatory response of the murine lung to inhaled endotoxin.
Article Snippet: .. Standard curves were generated and sample antigen concentrations were calculated using the Protein Microarray Analysis Tool (ProMAT), a custom freeware program that we developed specifically for this purpose (White et al., 2006). ..

Enzyme-linked Immunosorbent Assay:

Article Title: Protein secretion in human mammary epithelial cells following HER1 receptor activation: influence of HER2 and HER3 expression
Article Snippet: .. Spot fluorescent data were then processed and analyzed using Protein Microarray Analysis Tool (ProMAT) and ProMAT Calibrator [ - ], both of which are open-source, freeware programs (available at http://www.pnl.gov/statistics/ProMAT/ ) that we developed specifically for processing ELISA microarray data. ..

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.
Article Snippet: Fluorescence data were quantied using ScanArray Express soware (Perkin-Elmer). .. Data analysis was performed using ProMAT Calibrator and the Protein Microarray Analysis Tool (ProMAT).58,59 This publicly available soware normalizes data to a control protein, then ts the data to standard curves and ffer, milk and plasma using an ELISA protein microarray. ..

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Data, Statistics and ROC analyses Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Generated:

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Data, Statistics and ROC analyses Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Macrophage Responses to Silica Nanoparticles are Highly Conserved Across Particle Sizes
Article Snippet: The fluorescent images for the slides were obtained using ScanArray ExpressHT laser scanner and the spot intensity quantified using ScanArray Express (Perkin Elmer, Wellesley, MA). .. Standard curves were generated and sample antigen concentrations were calculated using the Protein Microarray Analysis Tool (ProMAT), which is a custom freeware program that we developed specifically for this use ( White et al. , 2006 ). . Gene expression and pathway analysis. .. Whole genome microarray analysis was performed using Affymetrix Mouse Genome 430A 2.0 chips (Affymetrix, Santa Clara, CA; 22,690 probesets).

Article Title: Diet-induced obesity reprograms the inflammatory response of the murine lung to inhaled endotoxin.
Article Snippet: .. Standard curves were generated and sample antigen concentrations were calculated using the Protein Microarray Analysis Tool (ProMAT), a custom freeware program that we developed specifically for this purpose (White et al., 2006). ..

Software:

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Plasma Biomarker Profiles Differ Depending on Breast Cancer Subtype but RANTES is Consistently Increased
Article Snippet: .. Data, Statistics and ROC analyses Standard curves from the ELISA microarray analyses were generated using the Protein Microarray Analysis Tool (ProMAT), a custom software program that we developed specifically for this use ( 17 ). ..

Article Title: Antibody microarrays for high-throughput, multianalyte analysis
Article Snippet: Enzyme-linked immunosorbent assay (ELISA) microarray technology promises to be a powerful tool for detecting and validating protein biomarkers, especially panels of biomarkers.. ELISA microarrays are capable of high-throughput analysis of multiple proteins using small sample volumes.. In this chapter we review the literature on the use of antibody microarrays for biomarker discovery and validation.

Control:

Article Title: Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.
Article Snippet: Fluorescence data were quantied using ScanArray Express soware (Perkin-Elmer). .. Data analysis was performed using ProMAT Calibrator and the Protein Microarray Analysis Tool (ProMAT).58,59 This publicly available soware normalizes data to a control protein, then ts the data to standard curves and ffer, milk and plasma using an ELISA protein microarray. ..

Clinical Proteomics:

Article Title: Development of an ELISA microarray assay for the sensitive and simultaneous detection of ten biodefense toxins.
Article Snippet: Fluorescence data were quantied using ScanArray Express soware (Perkin-Elmer). .. Data analysis was performed using ProMAT Calibrator and the Protein Microarray Analysis Tool (ProMAT).58,59 This publicly available soware normalizes data to a control protein, then ts the data to standard curves and ffer, milk and plasma using an ELISA protein microarray. ..

Concentration Assay:

Article Title: Susceptibility to quantum dot induced lung inflammation differs widely among the Collaborative Cross founder mouse strains
Article Snippet: .. Standard curves were then fit with the Protein Microarray Analysis Tool (ProMAT) using the four-parameter logistic curve-fitting option ( White et al., 2006 ) and used by ProMAT to generate antigen concentration values for the samples based on the adjusted spot fluorescent intensities. ..

Gene Expression:

Article Title: Macrophage Responses to Silica Nanoparticles are Highly Conserved Across Particle Sizes
Article Snippet: The fluorescent images for the slides were obtained using ScanArray ExpressHT laser scanner and the spot intensity quantified using ScanArray Express (Perkin Elmer, Wellesley, MA). .. Standard curves were generated and sample antigen concentrations were calculated using the Protein Microarray Analysis Tool (ProMAT), which is a custom freeware program that we developed specifically for this use ( White et al. , 2006 ). . Gene expression and pathway analysis. .. Whole genome microarray analysis was performed using Affymetrix Mouse Genome 430A 2.0 chips (Affymetrix, Santa Clara, CA; 22,690 probesets).



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Thermo Fisher human protein microarray catalog # 27055101
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Image Search Results


Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.

Journal: Journal of Virology

Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

doi: 10.1128/jvi.00478-25

Figure Lengend Snippet: Construction and characterization of the ASFV proteome microarray. In parallel with the controls, a total of 160 GST-tagged ASFV proteins were printed in triplicate onto a PATH substrate slide, generating 14 identical subarrays per slide. The slides were probed with an anti-GST antibody and a Cy3-labeled secondary antibody. (a) A representative subarray (upper) and the layout of the array (lower). (b) Histogram analysis of the fluorescence intensity of all the immobilized ASFV proteins based on their N-terminal GST tag probed with an anti-GST antibody and a fluorescently labeled secondary antibody. (c) Fluorescence intensity distribution of the spots of ASFV proteins and negative controls (including blank, BSA, IgG, IgM, and IgA) after probing with an anti-GST antibody and a fluorescently labeled secondary antibody. (d) Representative subarrays probed with sera from an ASFV-infected pig and a healthy pig. The IgG signals are shown in green. (e) Correlation analysis showing the repeated experimental results for the same serum sample.

Article Snippet: Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on PATH Protein Microarray Slides (GraceBio-Labs, Oregon, USA).

Techniques: Microarray, Labeling, Fluorescence, Infection

High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.

Journal: Journal of Virology

Article Title: An African swine fever virus-specific antibody reactome reveals antigens as potential candidates for vaccine development

doi: 10.1128/jvi.00478-25

Figure Lengend Snippet: High-throughput analysis of sera from ASFV-infected pigs using a proteome microarray. (a) Timelines of animal treatment and sample collection. Group A pigs ( n = 5) were infected with a virulent ASFV CN/GS 2018 strain at 1 HAD 50 . Three pigs died by 15 dpi, while the other two pigs developed clinical symptoms of ASF during the observation period but ultimately survived. Serum samples were collected at 3, 5, 7, 9, and 15 dpi. Group B pigs ( n = 5) inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF360-18R/DP71L/DP96R survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 0, 7, 13, and 17 dpi and at 7 dpc. Group C pigs inoculated with 10 4 HAD 50 of ASFV-GS-ΔMGF110/360-9L ( n = 6) survived the 17-day observation period and remained alive after being challenged with 10 2 HAD 50 of the parental virus during the 13-day observation period. Serum samples were collected at 7, 13, and 17 dpi and 7 dpc. (b) Uniform manifold approximation and projection (UMAP) of the 160-ASFV-protein-specific IgG signals in the serum samples from the three groups. Each point represents an individual serum sample. (c) The amounts of the IgG-positive ASFV proteins and the shared portion from groups B and C.

Article Snippet: Briefly, using a Super Marathon printer (Arrayjet, UK), identical protein arrays in a 2 × 7 subarray format were generated by printing affinity-purified 160 ASFV proteins, accompanied by negative (BSA and GST) and positive controls (anti-swine IgG [Novus Biologicals, USA, Cat# NBP1-97054], IgM [Novus Biologicals, USA, Cat# NBP1-96788], and IgA [Alpha Diagnostic International, USA, Cat# 20017-4-1]) and land markers, in triplicate, on PATH Protein Microarray Slides (GraceBio-Labs, Oregon, USA).

Techniques: High Throughput Screening Assay, Infection, Microarray, Virus

SIRT4 targets identified by Human Protein Microarray. A) Scan of Human Protein Microarray in the presence or absence (Control) of human recombinant SIRT4 protein. The pattern of red fluorescence spots (always in duplicate) on the control chip serves to facilitate specific protein identification following fluorescence excitation. Fluorescence spots specifically identified on the array incubated with SIRT4 represent potential SIRT4 interaction targets (exemplarily illustrated by the white arrow). B) List of proteins identified by protein-protein interaction with SIRT4 using a Human Protein Microarray. SIRT4, sirtuin 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Journal: Journal of Molecular and Cellular Cardiology Plus

Article Title: Sirtuin 4 accelerates heart failure development by enhancing reactive oxygen species-mediated profibrotic transcriptional signaling

doi: 10.1016/j.jmccpl.2025.100299

Figure Lengend Snippet: SIRT4 targets identified by Human Protein Microarray. A) Scan of Human Protein Microarray in the presence or absence (Control) of human recombinant SIRT4 protein. The pattern of red fluorescence spots (always in duplicate) on the control chip serves to facilitate specific protein identification following fluorescence excitation. Fluorescence spots specifically identified on the array incubated with SIRT4 represent potential SIRT4 interaction targets (exemplarily illustrated by the white arrow). B) List of proteins identified by protein-protein interaction with SIRT4 using a Human Protein Microarray. SIRT4, sirtuin 4. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)

Article Snippet: SIRT4 protein interaction was investigated using a Human Protein Microarray (Catalog # 27055101, Thermo Fisher Scientific, Germany) following the manufacturing protocol.

Techniques: Microarray, Control, Recombinant, Fluorescence, Incubation