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MJ Research automated heating block
Automated Heating Block, supplied by MJ Research, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/programmable+heating/programmable+heat+block/us07223902-431-7-11
Average 90 stars, based on 1 article reviews
automated heating block - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Incubation:

Article Title: Rapid detection of K-ras gene mutations in canine lung cancer using single-strand conformational polymorphism analysis.
Article Snippet: .. Using the thermostable DNA polymerase (Taq), 1 μg of DNA was incubated in the PCR reaction for 40 cycles of denaturation at 95°C, annealing at 55°C and polymerization at 72°C in a programmable heat block (MJ Research, Watertown, MA). ..

Article Title: RNA Crosslinking Methods
Article Snippet: .. The RNA-containing solutions are heated to 90 °C for 1 min in a programmable heating block (MJ Research) and then cooled to room temperature using a standard water bath over a period of approximately 20 min. Divalent metal ions, in this example 25 m M CaCl 2 , are then added and the RNAs incubated at 37 °C for 15–30 min to insure that as much of the RNA as possible has attained the native, folded form. .. The RNA-containing solutions are heated to 90 °C for 1 min in a programmable heating block (MJ Research) and then cooled to room temperature using a standard water bath over a period of approximately 20 min. Divalent metal ions, in this example 25 m M CaCl 2 , are then added and the RNAs incubated at 37 °C for 15–30 min to insure that as much of the RNA as possible has attained the native, folded form.

Polymerase Chain Reaction:

Article Title: Rapid detection of K-ras gene mutations in canine lung cancer using single-strand conformational polymorphism analysis.
Article Snippet: .. Using the thermostable DNA polymerase (Taq), 1 μg of DNA was incubated in the PCR reaction for 40 cycles of denaturation at 95°C, annealing at 55°C and polymerization at 72°C in a programmable heat block (MJ Research, Watertown, MA). ..

Article Title: Y chromosomal polymorphisms reveal founding lineages in the Finns and the Saami.
Article Snippet: .. The PCR was performed in a programmable heat block (MJ Research, Watertown, USA) as described earlier.20 After amplification, the PCR-product was run on 2% agarose gel for 1 h at 90 V to detect the insertions. .. The results were recorded on Polaroid 667 ISO 3000/36° film under UV illumination after staining with ethidium bromide.

Article Title: Molecular genetic characterization of both components of a dedifferentiated chondrosarcoma, with implications for its histogenesis
Article Snippet: .. Analysis of microsatellite markers was performed by polymerase chain reaction (PCR) on 100 ng of DNA as described by Weber and May11 using 1 Ci of [ 32P]dCTP in a total volume of 12 l. Thermal cycling was performed in a programmable heatblock (MJ Research, Watertown, MA, U.S.A.) consisting of 27 cycles with an annealing temperature of 55 C. The microsatellite markers used were selected because they map to chromosome regions reported to be involved in chondrosarcoma. ..

Blocking Assay:

Article Title: Rapid detection of K-ras gene mutations in canine lung cancer using single-strand conformational polymorphism analysis.
Article Snippet: .. Using the thermostable DNA polymerase (Taq), 1 μg of DNA was incubated in the PCR reaction for 40 cycles of denaturation at 95°C, annealing at 55°C and polymerization at 72°C in a programmable heat block (MJ Research, Watertown, MA). ..

Article Title: Loss of heterozygosity and DNA ploidy point to a diverging genetic mechanism in the origin of peripheral and central chondrosarcoma
Article Snippet: Chondrosarcomas are malignant cartilaginous tumors arising centrally in bone (central chondrosarcoma), or secondarily within the cartilaginous cap of a hereditary or sporadic exostosis (peripheral chondrosarcoma).. Loss of heterozygosity (LOH) was studied by microsatellite analysis at the loci harboring the EXT genes (implicated in hereditary multiple exostoses), the EXT-like genes, and at 9p21, 13q14, 17p13, and chromosome 10.. Nineteen of 20 peripheral chondrosarcomas showed LOH at all loci tested, while only 3 of 12 central chondrosarcomas exhibited LOH, restricted to 9p21, 10, 13q14, and 17p13.

Article Title: Y chromosomal polymorphisms reveal founding lineages in the Finns and the Saami.
Article Snippet: .. The PCR was performed in a programmable heat block (MJ Research, Watertown, USA) as described earlier.20 After amplification, the PCR-product was run on 2% agarose gel for 1 h at 90 V to detect the insertions. .. The results were recorded on Polaroid 667 ISO 3000/36° film under UV illumination after staining with ethidium bromide.

Article Title: Efficient amplification of light and heavy chain variable regions and construction of a non-immune phage scFv library.
Article Snippet: Non-immune phage scFv library is one of the most attractive resources for therapeutics, diagnostics and basic research.. As a matter of fact, quality of the library is limited by inefficient PCR cloning of antibody genes using degenerated primers.. PCR using this type of primers is difficult to optimize conditions for efficient amplification, and therefore causes loss of antibody diversities.

Article Title: RNA Crosslinking Methods
Article Snippet: .. The RNA-containing solutions are heated to 90 °C for 1 min in a programmable heating block (MJ Research) and then cooled to room temperature using a standard water bath over a period of approximately 20 min. Divalent metal ions, in this example 25 m M CaCl 2 , are then added and the RNAs incubated at 37 °C for 15–30 min to insure that as much of the RNA as possible has attained the native, folded form. .. The RNA-containing solutions are heated to 90 °C for 1 min in a programmable heating block (MJ Research) and then cooled to room temperature using a standard water bath over a period of approximately 20 min. Divalent metal ions, in this example 25 m M CaCl 2 , are then added and the RNAs incubated at 37 °C for 15–30 min to insure that as much of the RNA as possible has attained the native, folded form.

Article Title: Three novel spermatogenesis-specific zinc finger genes.
Article Snippet: We have cloned and characterized the expression, during spermatogenesis, of three novel zinc ¢nger genes (Zfp94, Zfp95, Zfp96).. Analysis of the deduced protein sequences reveals that all three molecules belong to the LeR family (leucine-rich zinc ¢ngers) and that ZFP95 contains a domain homologous to the Kru«ppel-associated box.. All three genes were found expressed at high levels in testis among other tissues, but testis-speci¢c transcripts were observed for Zfp95 and Zfp96.

Amplification:

Article Title: Y chromosomal polymorphisms reveal founding lineages in the Finns and the Saami.
Article Snippet: .. The PCR was performed in a programmable heat block (MJ Research, Watertown, USA) as described earlier.20 After amplification, the PCR-product was run on 2% agarose gel for 1 h at 90 V to detect the insertions. .. The results were recorded on Polaroid 667 ISO 3000/36° film under UV illumination after staining with ethidium bromide.

Agarose Gel Electrophoresis:

Article Title: Y chromosomal polymorphisms reveal founding lineages in the Finns and the Saami.
Article Snippet: .. The PCR was performed in a programmable heat block (MJ Research, Watertown, USA) as described earlier.20 After amplification, the PCR-product was run on 2% agarose gel for 1 h at 90 V to detect the insertions. .. The results were recorded on Polaroid 667 ISO 3000/36° film under UV illumination after staining with ethidium bromide.

other:

Article Title: Method of producing recombinant
Article Snippet: DNA amplification reaction mixture (25 μl) contained: reverse transcriptase reaction product (1 μl), 10×PCR buffer (2.5 μl, Promega Corp., Madison, Wis.), dNTPs (250 μM each), MgCl2 (2.0 mM), degenerate primers (250 pmol each), DNA polymerase (3 units, Stratagene, La Jolla, Calif.) and overlaid with mineral oil (25 μl).



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