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reverse transcriptase enzyme  (Promega)

 
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    Promega reverse transcriptase enzyme
    Reverse Transcriptase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/processive+reverse+transcriptase+enzyme/reverse+transcriptase+enzyme/pm37313503-86-23-26
    Average 90 stars, based on 1 article reviews
    reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars

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    other:

    Article Title: The intensity of the transcriptional response varies across infection with distinct viral strains in an insect host.
    Article Snippet: Briefly, RNA was isolated from whole bees using an RNeasy mini kit (Qiagen, Hilden, Germany). cDNA was synthesized from 800ng of RNA using oligo(dT)18 primers (Thermo Scientific, Waltham, MA, USA) and reverse transcriptase (M-MLV and Revertase, Promega, Mannheim, Germany) following the manufacturer’s protocol. cDNA was diluted 1:10x prior to qPCR reactions. qPCR was conducted using SYBRgreen Sensimix (Bioline, Luckenwalde, Germany) melt curve analysis to ensure the correct product had been amplified.

    Article Title: Maintaining Toll signaling in Drosophila brain is required to sustain autophagy for dopamine neuron survival
    Article Snippet: Total RNA (2–3 μg) from each sample was used for cDNA synthesis using reverse transcriptase (RTase) (Promega, USA) and oligo dT 18 primer (Intergrated DNA Technologies, USA) at 42°C for 60 min in a 25-μL reaction volume.

    Synthesized:

    Article Title: Mapping Arginase Expression with 18 F-Fluorinated Late-Generation Arginase Inhibitors Derived from Quaternary α-Amino Acids.
    Article Snippet: .. 62 No. 8 August 2021 AppliedBiosystems) according to themanufacturer’s instructions.Complementary DNAwas synthesized from equal amounts of RNAusing the Reverse Transcriptase System (Promega) and the following protocol: 10 min at 25 C, 45 min at 42 C, and 5 min at 99 C. Reverse-transcription polymerase chain reaction was performed with SYBR Green (Roche Diagnostics) and the following protocol, including a final step to generate themelting curve: 2 min at 95 C, 10min at 95 C, 453 (30 s at 95 C, 30 s at 60 C, and 30 s at 72 C), 30 s at 95 C, 30 s at 55 C, and 30 s at 95 C. The reverse-transcription polymerase chain reaction was performed in an Eco system (Illumina). ..

    Reverse Transcription:

    Article Title: Mapping Arginase Expression with 18 F-Fluorinated Late-Generation Arginase Inhibitors Derived from Quaternary α-Amino Acids.
    Article Snippet: .. 62 No. 8 August 2021 AppliedBiosystems) according to themanufacturer’s instructions.Complementary DNAwas synthesized from equal amounts of RNAusing the Reverse Transcriptase System (Promega) and the following protocol: 10 min at 25 C, 45 min at 42 C, and 5 min at 99 C. Reverse-transcription polymerase chain reaction was performed with SYBR Green (Roche Diagnostics) and the following protocol, including a final step to generate themelting curve: 2 min at 95 C, 10min at 95 C, 453 (30 s at 95 C, 30 s at 60 C, and 30 s at 72 C), 30 s at 95 C, 30 s at 55 C, and 30 s at 95 C. The reverse-transcription polymerase chain reaction was performed in an Eco system (Illumina). ..

    Article Title: Sphingomonas Paucimobilis -derived Extracellular Vesicles Reverse Aβ-induced Dysregulation of Neurotrophic Factors, Mitochondrial Function, and Inflammatory Factors through MeCP2-mediated Mechanism.
    Article Snippet: .. Two μg of total RNA was converted to cDNA using a reverse transcriptase system (Promega, Madison, WI, USA). .. The PCR was carried out using the CFX 96 Real-Time PCR System Detector (Bio-Rad Laboratories) in a 20 μl volume containing 4 μl of 1/8 diluted cDNA, 10 μl of 2X iQTM SYBR Green Supermix (Bio-Rad Laboratories, Foster City, CA, USA), and 1 μl each of 5 pmol/μl forward and reverse primers.

    Article Title: The EGR1-Artemin Axis in Keratinocytes Enhances the Innervation of Epidermal Sensory Neurons during Skin Inflammation Induced by House Dust Mite Extract from Dermatophagoidesfarinae.
    Article Snippet: Epidermal hyperinnervation is a critical feature of pruritus during skin inflammation.. However, the mechanisms underlying epidermal hyperinnervation are unclear.. This study investigates the role of the transcription factor EGR1 in epidermal innervation by utilizing wild-type (Egr1þ/þ) and Egr1-null (Egr1) mice topically applied Dermatophagoides farinae extract from dust mite.

    Article Title: Single-Cell Transcriptional Analysis of Lamina Propria Lymphocytes in the Jejunum Reveals Innate Lymphoid Cell-like Cells in Pigs.
    Article Snippet: RESEARCH ARTICLE | NOVEMBER 17 2023 Single-Cell Transcriptional Analysis of Lamina Propria Lymphocytes in the Jejunum Reveals Innate Lymphoid Cell–like Cells in Pigs Junhong Wang; ... et. al J Immunol (2024) 212 (1): 130–142. https://doi.org/10.4049/jimmunol.2300463 Related Content Senescent Jejunal Mast Cells and Eosinophils in the Mouse Preferentially Translocate to the Spleen and Draining Lymph Node, Respectively, During the Recovery Phase of Helminth Infection J Immunol (July,2000) Cathelicidin-WA Improves Intestinal Epithelial Barrier Function and Enhances Host Defense against Enterohemorrhagic Escherichia coli O157:H7 Infection J Immunol (February,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/212/1/130/1650261/ji2300463.pdf by guest on 03 M ay 2025

    Article Title: Anticancer actions of carnosine in cellular models of prostate cancer.
    Article Snippet: Total RNA was extracted using the RNeasy Mini Extraction Kit (QIAGEN) according to the manufacturer's protocol and the concentration of extracted RNA was measured on a NanoDrop spectrophotometer (Thermo Fisher). .. To synthesize cDNA, 2 μg of RNA in nuclease-free water (final volume 9 μL) and 1 μL of oligo dT (Promega) were heated at 70°C for 5 min. A mix containing 5 μL of RT buffer (Promega), 1 μL of Reverse Transcriptase enzyme (Promega), 0.7 μL of RNasin (Promega), 1 μL of dNTPs (Promega) and 7.3 μL of nuclease-free water were then added to the reaction and incubated at 40°C for 1 h. The reaction was heated at 95°C for 5 min and frozen immediately at −20°C. .. The RT-qPCR reaction was performed in a final volume of 13 μL by mixing 1 μL of cDNA, 6.25 μL of iTaq Universal SYBR Green supermix (Bio-Rad), 0.5 μL of forward and reverse primer (10 pM) and 4.75 μL of nuclease-free water.

    Article Title: Hermetia illucens -Derived Chitosan: A Promising Immunomodulatory Agent for Applications in Biomedical Fields.
    Article Snippet: .. The extracted mRNA was employed to synthesize complementary DNA (cDNA), using reverse transcriptase enzyme (Promega). ..

    Polymerase Chain Reaction:

    Article Title: Mapping Arginase Expression with 18 F-Fluorinated Late-Generation Arginase Inhibitors Derived from Quaternary α-Amino Acids.
    Article Snippet: .. 62 No. 8 August 2021 AppliedBiosystems) according to themanufacturer’s instructions.Complementary DNAwas synthesized from equal amounts of RNAusing the Reverse Transcriptase System (Promega) and the following protocol: 10 min at 25 C, 45 min at 42 C, and 5 min at 99 C. Reverse-transcription polymerase chain reaction was performed with SYBR Green (Roche Diagnostics) and the following protocol, including a final step to generate themelting curve: 2 min at 95 C, 10min at 95 C, 453 (30 s at 95 C, 30 s at 60 C, and 30 s at 72 C), 30 s at 95 C, 30 s at 55 C, and 30 s at 95 C. The reverse-transcription polymerase chain reaction was performed in an Eco system (Illumina). ..

    SYBR Green Assay:

    Article Title: Mapping Arginase Expression with 18 F-Fluorinated Late-Generation Arginase Inhibitors Derived from Quaternary α-Amino Acids.
    Article Snippet: .. 62 No. 8 August 2021 AppliedBiosystems) according to themanufacturer’s instructions.Complementary DNAwas synthesized from equal amounts of RNAusing the Reverse Transcriptase System (Promega) and the following protocol: 10 min at 25 C, 45 min at 42 C, and 5 min at 99 C. Reverse-transcription polymerase chain reaction was performed with SYBR Green (Roche Diagnostics) and the following protocol, including a final step to generate themelting curve: 2 min at 95 C, 10min at 95 C, 453 (30 s at 95 C, 30 s at 60 C, and 30 s at 72 C), 30 s at 95 C, 30 s at 55 C, and 30 s at 95 C. The reverse-transcription polymerase chain reaction was performed in an Eco system (Illumina). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: The EGR1-Artemin Axis in Keratinocytes Enhances the Innervation of Epidermal Sensory Neurons during Skin Inflammation Induced by House Dust Mite Extract from Dermatophagoidesfarinae.
    Article Snippet: Epidermal hyperinnervation is a critical feature of pruritus during skin inflammation.. However, the mechanisms underlying epidermal hyperinnervation are unclear.. This study investigates the role of the transcription factor EGR1 in epidermal innervation by utilizing wild-type (Egr1þ/þ) and Egr1-null (Egr1) mice topically applied Dermatophagoides farinae extract from dust mite.

    Virus:

    Article Title: Single-Cell Transcriptional Analysis of Lamina Propria Lymphocytes in the Jejunum Reveals Innate Lymphoid Cell-like Cells in Pigs.
    Article Snippet: RESEARCH ARTICLE | NOVEMBER 17 2023 Single-Cell Transcriptional Analysis of Lamina Propria Lymphocytes in the Jejunum Reveals Innate Lymphoid Cell–like Cells in Pigs Junhong Wang; ... et. al J Immunol (2024) 212 (1): 130–142. https://doi.org/10.4049/jimmunol.2300463 Related Content Senescent Jejunal Mast Cells and Eosinophils in the Mouse Preferentially Translocate to the Spleen and Draining Lymph Node, Respectively, During the Recovery Phase of Helminth Infection J Immunol (July,2000) Cathelicidin-WA Improves Intestinal Epithelial Barrier Function and Enhances Host Defense against Enterohemorrhagic Escherichia coli O157:H7 Infection J Immunol (February,2017) D ow nloaded from http://journals.aai.org/jim m unol/article-pdf/212/1/130/1650261/ji2300463.pdf by guest on 03 M ay 2025

    Incubation:

    Article Title: Anticancer actions of carnosine in cellular models of prostate cancer.
    Article Snippet: Total RNA was extracted using the RNeasy Mini Extraction Kit (QIAGEN) according to the manufacturer's protocol and the concentration of extracted RNA was measured on a NanoDrop spectrophotometer (Thermo Fisher). .. To synthesize cDNA, 2 μg of RNA in nuclease-free water (final volume 9 μL) and 1 μL of oligo dT (Promega) were heated at 70°C for 5 min. A mix containing 5 μL of RT buffer (Promega), 1 μL of Reverse Transcriptase enzyme (Promega), 0.7 μL of RNasin (Promega), 1 μL of dNTPs (Promega) and 7.3 μL of nuclease-free water were then added to the reaction and incubated at 40°C for 1 h. The reaction was heated at 95°C for 5 min and frozen immediately at −20°C. .. The RT-qPCR reaction was performed in a final volume of 13 μL by mixing 1 μL of cDNA, 6.25 μL of iTaq Universal SYBR Green supermix (Bio-Rad), 0.5 μL of forward and reverse primer (10 pM) and 4.75 μL of nuclease-free water.



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