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primescript ii high fidelity reverse transcription pcr rt pcr kit  (TaKaRa)


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    Structured Review

    TaKaRa primescript ii high fidelity reverse transcription pcr rt pcr kit
    Primescript Ii High Fidelity Reverse Transcription Pcr Rt Pcr Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 516 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescript+rt+reagent+kit/PrimeScript+High+Fidelity+RT-PCR+Kit/pm37806084-94-40-48
    Average 96 stars, based on 516 article reviews
    primescript ii high fidelity reverse transcription pcr rt pcr kit - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: TRPA1 for Butterfly Eyespot Formation.
    Article Snippet: We used NucleoSpin RNA Plus (Takara Bio, Kusatsu, Shiga, Japan) to extract RNA from the dissected tissues in accordance with the manufacturer’s protocol. .. RT-PCR was performed using a PrimeScript II High-Fidelity RT-PCR Kit (Takara Bio). ..

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy
    Article Snippet: Total RNA was extracted from HCAECs using TRIzol reagent (15596026CN; Invitrogen, USA). cDNA was synthesized using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (6210A; Takara, Japan). .. PCR was performed using PrimeScriptTM II High Fidelity RT-PCR Kit (R023A; Takara, Japan). .. Gene expression was quantified on a QuantStudio 7 Flex using SYBR Select (Thermo Fisher Scientific, USA) and the 2- ΔΔ Ct method, normalizing to β-actin.

    Article Title: Tetrodotoxin-resistant skeletal muscle sodium channel Na v 1.4 of German newts with low toxin levels.
    Article Snippet: RNA was resuspended in RNase-free water and quantified using the QuantiFluor RNA System (Promega). .. First-strand cDNA was synthesized using the PrimeScript II High Fidelity RT-PCR kit (TaKaRa) with random hexamers according to the manufacturer's instructions. .. Nav1.4 coding sequences were amplified from the cDNA described above using newt-specific primers (Supplementary Table S1) designed in this study based on nucleotide alignments of 10 salamander species (Hanifin and Gilly, 2015), together with KOD ONE DNA polymerase (TOYOBO).

    Article Title: Tomato lipocalins mediate ABA (abscisic acid)- and ethylene-dependent regulation of stress tolerance and fruit ripening
    Article Snippet: Total RNA from all tissues was extracted using the RNeasy Plant Mini Kit (QIAGEN Co., Ltd., Germany), following the manufacturer’s protocol. .. This RNA was then used for reverse transcription, according to the instruction manual of the PrimeScriptTM High Fidelity RT-PCR Kit (#R0022A, TaKaRa Co., Ltd., Japan). .. The qRT-PCR of SlTIL1 , SlTIL2 , SlPDS , and SlPSY1 transcripts was carried out using TB Green® Premix Ex TaqTM II (#RR820A; TaKaRa) in a 25 μl reaction volume, consisting of 12.5 μl TB Green mix, 0.5 μl of 20 μM of each primer (Supplementary Table S1), 9 μl of distilled water, and 2.5 μl of cDNA (from total RNA at 1,000 ng μl −1 ).

    Article Title: ASGR1 induces neutrophil extracellular traps formation to exacerbate sepsis-induced myocardial injury and cardiac dysfunction through SLC7A11 ubiquitination
    Article Snippet: Protein bands were visualized using the enhanced 317 chemiluminescence reagent (Millipore). .. Quantitative real-time PCR (qRT-PCR) was conducted by PrimeScriptTM II High Fidelity RT-PCR Kit 323 Takara, Japan) as previously described. .. 318 RNA isolation and quantitative real-time PCR 319 Total ribonucleic acid (RNA) was extracted using TRIzol reagent (Cat#15596018; Invitrogen Life 320 Technologies, Carlsbad, CA, USA) from tissues according to the manufacturer's instructions.

    Article Title: A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis
    Article Snippet: .. A cDNA library was synthesized from the total RNA of RW-strain embryos at 24 hpf using the PrimeScript II High Fidelity RT-PCR (reverse transcription polymerase chain reaction) Kit (TaKaRa) and the oligo dT primer adding adapter sequence (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′). ..

    Article Title: Human papillomavirus E7 inhibits immune responses in keratinocytes by activating HTRA1-mediated mitophagy
    Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM High Fidelity RT-PCR Kit (cat. no. R022A; Takara Bio, Inc.) according to the manufacturer's protocol. .. First-strand cDNA was synthesized using the PrimeScript High Fidelity RT-PCR Kit (cat. no. R022A; Takara Bio, Inc.) according to the manufacturer's protocol.

    Article Title: TRPA1 for Butterfly Eyespot Formation
    Article Snippet: We used NucleoSpin RNA Plus (Takara Bio, Kusatsu, Shiga, Japan) to extract RNA from the dissected tissues in accordance with the manufacturer’s protocol. .. RT-PCR was performed using a PrimeScript II High-Fidelity RT-PCR Kit (Takara Bio). ..

    Polymerase Chain Reaction:

    Article Title: MPC1 promotes the damage of human coronary endothelial cells in macrolide-resistant mycoplasma pneumoniae via inhibiting mitophagy
    Article Snippet: Total RNA was extracted from HCAECs using TRIzol reagent (15596026CN; Invitrogen, USA). cDNA was synthesized using a PrimeScriptTM II 1st Strand cDNA Synthesis Kit (6210A; Takara, Japan). .. PCR was performed using PrimeScriptTM II High Fidelity RT-PCR Kit (R023A; Takara, Japan). .. Gene expression was quantified on a QuantStudio 7 Flex using SYBR Select (Thermo Fisher Scientific, USA) and the 2- ΔΔ Ct method, normalizing to β-actin.

    Article Title: A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis
    Article Snippet: .. A cDNA library was synthesized from the total RNA of RW-strain embryos at 24 hpf using the PrimeScript II High Fidelity RT-PCR (reverse transcription polymerase chain reaction) Kit (TaKaRa) and the oligo dT primer adding adapter sequence (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′). ..

    Synthesized:

    Article Title: Tetrodotoxin-resistant skeletal muscle sodium channel Na v 1.4 of German newts with low toxin levels.
    Article Snippet: RNA was resuspended in RNase-free water and quantified using the QuantiFluor RNA System (Promega). .. First-strand cDNA was synthesized using the PrimeScript II High Fidelity RT-PCR kit (TaKaRa) with random hexamers according to the manufacturer's instructions. .. Nav1.4 coding sequences were amplified from the cDNA described above using newt-specific primers (Supplementary Table S1) designed in this study based on nucleotide alignments of 10 salamander species (Hanifin and Gilly, 2015), together with KOD ONE DNA polymerase (TOYOBO).

    Article Title: A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis
    Article Snippet: .. A cDNA library was synthesized from the total RNA of RW-strain embryos at 24 hpf using the PrimeScript II High Fidelity RT-PCR (reverse transcription polymerase chain reaction) Kit (TaKaRa) and the oligo dT primer adding adapter sequence (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′). ..

    Article Title: Human papillomavirus E7 inhibits immune responses in keratinocytes by activating HTRA1-mediated mitophagy
    Article Snippet: .. First-strand cDNA was synthesized using the PrimeScriptTM High Fidelity RT-PCR Kit (cat. no. R022A; Takara Bio, Inc.) according to the manufacturer's protocol. .. First-strand cDNA was synthesized using the PrimeScript High Fidelity RT-PCR Kit (cat. no. R022A; Takara Bio, Inc.) according to the manufacturer's protocol.

    Reverse Transcription:

    Article Title: Tomato lipocalins mediate ABA (abscisic acid)- and ethylene-dependent regulation of stress tolerance and fruit ripening
    Article Snippet: Total RNA from all tissues was extracted using the RNeasy Plant Mini Kit (QIAGEN Co., Ltd., Germany), following the manufacturer’s protocol. .. This RNA was then used for reverse transcription, according to the instruction manual of the PrimeScriptTM High Fidelity RT-PCR Kit (#R0022A, TaKaRa Co., Ltd., Japan). .. The qRT-PCR of SlTIL1 , SlTIL2 , SlPDS , and SlPSY1 transcripts was carried out using TB Green® Premix Ex TaqTM II (#RR820A; TaKaRa) in a 25 μl reaction volume, consisting of 12.5 μl TB Green mix, 0.5 μl of 20 μM of each primer (Supplementary Table S1), 9 μl of distilled water, and 2.5 μl of cDNA (from total RNA at 1,000 ng μl −1 ).

    Article Title: A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis
    Article Snippet: .. A cDNA library was synthesized from the total RNA of RW-strain embryos at 24 hpf using the PrimeScript II High Fidelity RT-PCR (reverse transcription polymerase chain reaction) Kit (TaKaRa) and the oligo dT primer adding adapter sequence (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′). ..

    Real-time Polymerase Chain Reaction:

    Article Title: ASGR1 induces neutrophil extracellular traps formation to exacerbate sepsis-induced myocardial injury and cardiac dysfunction through SLC7A11 ubiquitination
    Article Snippet: Protein bands were visualized using the enhanced 317 chemiluminescence reagent (Millipore). .. Quantitative real-time PCR (qRT-PCR) was conducted by PrimeScriptTM II High Fidelity RT-PCR Kit 323 Takara, Japan) as previously described. .. 318 RNA isolation and quantitative real-time PCR 319 Total ribonucleic acid (RNA) was extracted using TRIzol reagent (Cat#15596018; Invitrogen Life 320 Technologies, Carlsbad, CA, USA) from tissues according to the manufacturer's instructions.

    cDNA Library Assay:

    Article Title: A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis
    Article Snippet: .. A cDNA library was synthesized from the total RNA of RW-strain embryos at 24 hpf using the PrimeScript II High Fidelity RT-PCR (reverse transcription polymerase chain reaction) Kit (TaKaRa) and the oligo dT primer adding adapter sequence (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′). ..

    Sequencing:

    Article Title: A cross-tissue physicochemical causal chain underlying vertebrate mandibular morphogenesis
    Article Snippet: .. A cDNA library was synthesized from the total RNA of RW-strain embryos at 24 hpf using the PrimeScript II High Fidelity RT-PCR (reverse transcription polymerase chain reaction) Kit (TaKaRa) and the oligo dT primer adding adapter sequence (5′-CTGATCTAGAGGTACCGGATCCTTTTTTTTTTTTTTT-3′). ..



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