prime script rt reagent kit (Promega)

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Promega prime script rt reagent kit
Prime Script Rt Reagent Kit, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/prime+script+rt+master+kit/prime+script+rt+reagent+kit/pmc07279159-184-6-11
Average 90 stars, based on 1 article reviews

prime script rt reagent kit - by Bioz Stars, 2026-09

90/100 stars

Images

other:

Article Title: Identification of pyroptosis-related long non-coding RNAs with prognosis and therapy in lung squamous cell carcinoma
Article Snippet: Then cDNA was synthesized using Prime-Script RT Kit (Promega Corporation, Madison, USA) for reverse transcription and Promega SYBR-Green PCR Master Mix (Promega Corporation, Madison, USA) for qRT-PCR on Roche LightCycler480 II real-time PCR system (Roche, Germany).

Article Title: The antihypertensive felodipine shows synergistic activity with immune checkpoint blockade and inhibits tumor growth via NFAT1 in LUSC
Article Snippet: After 48 h, the cells were collected, and the total RNA was extracted with a TRIzol reagent (Invitrogen, USA). cDNA was reverse-transcribed using a Prime-Script RT reagent Kit (Promega, Madison, WI, USA).

Article Title: 17-hydroxy-jolkinolide B potentiated CTLA4ab therapy through targeting tumor suppression and immune activation by downregulating PD-L1 expression in lung adenocarcinoma
Article Snippet: Prime-Script RT reagent Kit (Promega, Madison, WI, USA) was used to transform the collected RNA into cDNA.

Synthesized:

Article Title: PTRF/Cavin-1 enhances chemo-resistance and promotes temozolomide efflux through extracellular vesicles in glioblastoma
Article Snippet: .. The upper aqueous phase was collected to a 1.5 ml tube, and an equal volume of isopropanol was added into it. cDNAs were synthesized using the Prime Script RT Kit (Promega; Madison, 498 WI, USA), according to the manufacturer's protocols. qRT-PCR analyses were performed using the SYBR Premix Ex Taq kit (Takara), employing GAPDH as an internal reference. ..

Article Title: Autocross-linked hyaluronic acid gel and adipose-derived mesenchymal stem cell composites for the treatment intrauterine adhesions.
Article Snippet: RNA concentration (OD at 260 nm) and purity (OD260/280) were measured by a NanoDrop 2000c (Thermo Scientific, Bonn, Germany). .. The quality of the RNA was evaluated using spectrophotometry and denaturing agarose gel electrophoresis. cDNA was synthesized from 1 mg of purified total RNA using a Prime Script RT reagent kit (Promega) according to the manufacturer's instructions. .. Quantitative polymerase chain reactions were performed using an Applied Biosystems 7300 Fast real-time polymerase chain reaction system.

Quantitative RT-PCR:

Spectrophotometry:

Agarose Gel Electrophoresis:

Purification:

Generated:

Article Title: Hypoxia Inhibits Osteogenesis and Promotes Adipogenesis of Fibroblast-like Synoviocytes via Upregulation of Leptin in Patients with Rheumatoid Arthritis
Article Snippet: Briefly, total RNA was extracted using an RNAiso plus kit (Takara, Tokyo, Japan). .. Complementary DNA (cDNA) was generated from 1 μ g total RNA using a Prime Script RT Reagent Kit (Promega, Corporation, Wisconsin, USA). .. A SYBR Premix Ex Taq II kit (Takara, Liaoning, China) was used to quantify mRNA levels of interest genes in the ABI7500 Real-Time PCR System.

Real-time Polymerase Chain Reaction:

Article Title: Attenuation of Lipopolysaccharide-induced Liver Injury by Bone Marrow Mesenchymal Stem Cells via Inhibiting the NLRP3 Inflammasome and Hepatocyte Pyroptosis
Article Snippet: Background: The transplantation of bone marrow mesenchymal cells (BMSCs) has been shown to be an effective mean for treating sepsis-related organ damage.. Pytoptotic cell death, in turn, has recently been identified as a key driver of sepsis-related damage.. At present, there are few studies on the effect of BMSC transplantation on pyroptotic cell death.

Review