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Promega poly(a) tract system ii
Poly(a) Tract System Ii, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/polya+selection+method/poly+a++tract+system/pm14736877-75-7-16
Average 90 stars, based on 1 article reviews
poly(a) tract system ii - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Thioredoxin from the Indianmeal Moth Plodia interpunctella : Cloning and Test of the Allergenic Potential in Mice
Article Snippet: Poly(A) + RNA was prepared using the Poly(A)Ttract system (Promega, Madison, WI, USA) with a yield of 4.9 μg from 4 mg of total RNA.

Article Title: Up-regulation of Apoptosis Inhibitory Protein IAP-2 by Hypoxia
Article Snippet: Total RNA was extracted from control or experimental cells with TRI reagent (Molecular Research Center, Cincinnati, OH). mRNA was subsequently purified with poly(A)Tract (Promega, Madison, MI).

Article Title: Up-regulation of Apoptosis Inhibitory Protein IAP-2 by Hypoxia
Article Snippet: Northern Hybridization Total RNA was extracted from control or experimental cells with TRI reagent (Molecular Research Center, Cincinnati, OH). mRNA was subsequently purified with poly(A)Tract (Promega, Madison, MI).

Article Title: Liar, a novel Lyn-binding nuclear/cytoplasmic shuttling protein that influences erythropoietin-induced differentiation.
Article Snippet: Erythropoiesis is primarily controlled by erythropoietin (Epo), which stimulates proliferation, differentiation, and survival of erythroid precursors.. We have previously shown that the tyrosine kinase Lyn is critical for transducing differentiation signals emanating from the activated Epo receptor.. A yeast 2-hybrid screen for downstream effectors of Lyn identified a novel protein, Liar (Lyn-interacting ankyrin repeat), which forms a multiprotein complex with Lyn and HS1 in erythroid cells.

Article Title: Circadian control of mRNA polyadenylation dynamics regulates rhythmic protein expression
Article Snippet: Quantitative PCR (qPCR) Total RNAs were extracted as described above using Trizol reagent (Invitrogen), and then poly(A)-enriched RNAs were extracted by Poly(A)Tract system (Promega).

Isolation:

Article Title: Recombinant porcine adenovirus vector
Article Snippet: .. Poly A (mRNA) was isolated from total RNA by the use of the Poly AT tract System (Promega, USA). ..

Article Title: Altered biochemical profile and gene expression in aflatoxin B-1-transformed C3H10T1/2 cells.
Article Snippet: A transformed cell line 7SA, obtained by transformation of C3H10T1/2 cells with in vitro activated aflatoxin B, (AFB,), was used to investigate biochemical and molecular alterations associated with transformation by AFB,.. 7SA cells demonstrate an altered biochemical phenotype characterized by alterations in phase I and phase II enzymes in a manner that would allow these cells to survive in a hostile chemical environment.. Investigations of the molecular basis of transformation revealed no mutations in codons 12/13 and 61 of ras genes (Ha-, Kiand N-ras) and in exons 5, 6, 7 and 8 of p53 tumor suppressor gene.

Purification:

Article Title: Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
Article Snippet: Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites.. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom.. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using recombinant DNA technology.

Magnetic Beads:

Article Title: Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
Article Snippet: Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites.. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom.. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using recombinant DNA technology.

Rapid Amplification of cDNA Ends:

Article Title: Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
Article Snippet: Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites.. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom.. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using recombinant DNA technology.

Synthesized:

Article Title: Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
Article Snippet: Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites.. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom.. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using recombinant DNA technology.

Modification:

Article Title: Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
Article Snippet: Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites.. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom.. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using recombinant DNA technology.

Reverse Transcription:

Article Title: Albocollagenase, a novel recombinant P-III snake venom metalloproteinase from green pit viper (Cryptelytrops albolabris), digests collagen and inhibits platelet aggregation.
Article Snippet: Molecular cloning and functional characterization of P-III snake venom metalloproteinases (SVMPs) will give us deeper insights in the pathogenesis of viper bites.. This may lead to novel therapy for venom-induced local tissue damages, the complication refractory to current antivenom.. The aim of this study was to elucidate the in vitro activities of a new SVMP from the green pit viper (GPV) using recombinant DNA technology.



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