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lambda phage dna (Promega)

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Promega lambda phage dna
Lambda Phage Dna, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/phage+lambda+dna/lambda+phage+dna/pmc07452558-39-5-8
Average 90 stars, based on 1 article reviews

lambda phage dna - by Bioz Stars, 2026-09

90/100 stars

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Article Title: A deletion at the X-linked ARHGAP36 gene locus is associated with the orange coloration of tortoiseshell and calico cats.
Article Snippet: WGBS libraries were prepared using the post-bisulfite adapter taggingmethod.60 Two hundred ng of genomic DNA spiked with 2 ng lambda phage DNA (Promega) was subjected to bisulfite conversion using EZMethylation-Gold Kit (Zymo Research).

Article Title: Systems and methods for improved nanopore-based analysis of nucleic acids
Article Snippet: The lambda phage DNA (GenBank J02459.1) was obtained from Promega.

Article Title: A deletion at the X-linked ARHGAP36 gene locus is associated with the orange coloration of tortoiseshell and calico cats
Article Snippet: Genomic DNA spiked with 2 ng lambda phage DNA (Promega) was subjected to bisulfite conversion.

Control:

Article Title: Circulating cell-free DNA methylation patterns indicate cellular sources of allograft injury after liver transplant
Article Snippet: Genomic DNA was fragmented via sonication using a Covaris M220 instrument to the recommended 150–200 base pairs before library preparation. .. Lambda phage DNA (Promega Corporation) was also fragmented and included as a spike-in to all DNA samples at 0.5%w/w, serving as an internal unmethylated control. ..

Article Title: Circulating cell-free DNA methylation patterns indicate cellular sources of allograft injury after liver transplant.
Article Snippet: Genomic DNAwas fragmented via sonication using a Covaris M220 instrument to the recommended 150–200 base pairs before library preparation. .. Lambda phage DNA (Promega Corporation)wasalso fragmented and included as a spike-in to allDNA samples at 0.5%w/w, serving as an internal unmethylated control. ..

Article Title: Circulating, cell-free methylated DNA indicates cellular sources of allograft injury after liver transplant
Article Snippet: Genomic DNA was fragmented via sonication using a Covaris M220 instrument to the recommended 150-200 base pairs before library preparation. .. Lambda phage DNA (Promega Corporation) was also fragmented and included as a spike-in to all DNA samples at 0.5%w/w, serving as an internal unmethylated control. ..

Incubation:

Article Title: Joint single-cell profiling resolves 5mC and 5hmC and reveals their distinct gene regulatory effects
Article Snippet: .. For methylating lambda phage DNA (Promega) at CG sites, the DNA was first incubated with M.SssI methylase (New England Biolabs, M0226M) for 120 min in 50-μl reaction (1.0 μl of 20 unit/μl M.SssI and 1.0 μl of 32 mM S-adenosylmethionine (SAM), and additional M.SssI (0.5 μl) and SAM (1.0 μl) was supplemented to treat DNA for another 240 min 30 . .. The enzyme was deactivated by incubation at 65°C for 20min, and methylated lambda DNA was purified with 1.6x homebrew solid phase reversible immobilization (SPRI) beads (1ml Sera-Mag SpeedBeads (GE Healthcare), 9 g PEG 8000 (Sigma), 10 ml 5M NaCl, 500μl 1M Tris-HCl pH 8.0 (Invitrogen), 100 μl 0.5M EDTA pH 8.0 (Invitrogen)).

Sonication:

Article Title: Evolutionary conservation of embryonic DNA methylome remodelling in distantly related teleost species.
Article Snippet: .. Unmethyla ted lambda phage DNA (0.5%; Promega, Madison, WI, USA) was spiked into each DNA sample before the DNA was sonicated to an average of 300 bp. .. DNA was then purified using AMPure XP beads (Beckman Coulter, Lane Cove, NSW, Australia) and bisulfite converted using the EZ DNA MethylationGold Kit (Zymo Research, CA, USA), following manufacturer’s instructions.