eif 4e full length (Addgene inc)
Addgene inc is a verified supplier 
93
Structured Review
Addgene inc
eif 4e full length
Eif 4e Full Length, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pha+eif4e/pHA-eIF4E+(Plasmid+%2317343)/pmc03979714-28-2-13
Average 93 stars, based on 20 article reviews
Eif 4e Full Length, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 20 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pha+eif4e/pHA-eIF4E+(Plasmid+%2317343)/pmc03979714-28-2-13
Average 93 stars, based on 20 article reviews
eif 4e full length - by Bioz Stars,
2026-11
93/100 stars
Images
1) Product Images from "MNK1-Induced eIF-4E Phosphorylation in Myeloma Cells: A Pathway Mediating IL-6-Induced Expansion and Expression of Genes Involved in Metabolic and Proteotoxic Responses"
Article Title: MNK1-Induced eIF-4E Phosphorylation in Myeloma Cells: A Pathway Mediating IL-6-Induced Expansion and Expression of Genes Involved in Metabolic and Proteotoxic Responses
Journal: PLoS ONE
doi: 10.1371/journal.pone.0094011
Figure Legend Snippet: A ) MM lines exposed to IL-6 (100 U/ml) for 3 hrs followed by immunoblot assay for phospho-MNK and total MNK expression. Fold increase is determined by densitometric ratio of MNK-P/MNK-total and represents the mean of 3 independent experiments. * denotes IL-6-induced increase is statistically significant (p<0.05). B ) ANBL-6 cells exposed to IL-6 for 0, 60, 120 or 180 mins, followed by immunoblot assay. C ) Primary cells from 4 patients exposed to IL-6 or media (C) followed by immunoblot assay. D ) ANBL-6 cells treated with IL-6 for increasing durations, followed by immunoprecipitation with anti-MNK1, MNK2 or control IgG. Immunoprecipitates tested for phosphorylation of eIF-4E in vitro.
Techniques Used: Western Blot, Expressing, Immunoprecipitation, In Vitro
Figure Legend Snippet: A ) ANBL-6 or 8226 cells transfected with empty vector (EV), serine-to-alanine phosphodefective eIF-4E (SA), serine-to-aspartic acid phosphomimetic eIF-4E (ASP) or wild type (WT) eIF-4E, followed by immunoblot assay. Transfected (transfex) vs endogenous (endog) eIF-4E denoted. B ) Above described ANBL-6 and 8226 transfected cell lines treated +/− IL-6 (100 U/ml) for varied durations and viable cell recovery enumerated. Results are relative cell growth compared to number of cells present at time 0 (mean+/−SD (n = 3)). * denotes statistically significant (p<0.05) alteration in cell recovery versus empty vector-transfected cells.
Techniques Used: Transfection, Plasmid Preparation, Western Blot
Figure Legend Snippet: A ) 8226 cells injected SQ and mice randomized to control (C) group, low dose CGP57380 (LD, 20 mg/kg) or high dose (HD, 50 mg/kg). Treatment is daily IP injections. At day +4 and +7 of treatment, tumors were measured. Data is tumor volume, mean+/−SD (n = 8). * = significantly (p<0.05) smaller tumor vs control. B and C ) Individual control (C), high dose (HD)-treated or low dose (LD)-treated tumors harvested after 3 days of treatment for Western blot, assaying phospho-eIF-4E, total eIF-4E, GAPDH and PARP that has been cleaved by caspase (AB #954 from Cell Signaling). D ) Mice challenged SQ with 8226 cells transfected with EV (right flank) and 8226 cells transfected with dominant negative eIF-4E (left flank). A separate cohort of mice were challenged with non-transfected parental 8226 cells. Tumor growth is mean+/−SD tumor volume, n = 8.
Techniques Used: Injection, Western Blot, Transfection, Dominant Negative Mutation
Figure Legend Snippet: A ) AHA incorporation is tested in MM cells stimulated with IL-6 for 30, 60, 120 or 180 mins. In B ), AHA incorporation is tested in IL-6-stimulated EV-, WT or mutant SA eIF-4E-transfected MM cells. In C ), EV or SA mutant-expressing cells are pre-treated +/− pp242, followed by IL-6. Bar graphs under panels are means+/−SD of 4 separate experiments. * denotes statistically significant increase in AHA incorporation induced by IL-6 in A and B and decrease versus IL-6-stimulated control in C). D ) Lambda light chain ELISA assay in MM cell lysates, comparing EV- vs mutant eIF-4E-transfected cells +/− IL-6 and +/− pp242. Data are means+/−SD, n = 4. * denotes statistically significant difference from corresponding controls.
Techniques Used: Mutagenesis, Transfection, Expressing, Enzyme-linked Immunosorbent Assay
Figure Legend Snippet: A ) Different categories of 166 genes demonstrating significantly inhibited translation in mutant-expressing cells. B ) Immunoblot analysis of protein expression in EV-, WT and mutant (MU)-expressing MM cells. C ) Immunoblot analysis of protein expression in EV vs mutant (MU) eIF-4E-expressing MM cells +/− IL-6 for 48 hrs.
Techniques Used: Mutagenesis, Expressing, Western Blot
Related Articles
other:Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. Expressing:Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Construct:Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Plasmid Preparation:Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Sequencing:Article Title: Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms. Article Snippet: Get e-Alerts ARTICLE | February 19, 2025 Sortase-Mediated Fluorescent Labeling of eIF4E for Investigating Translation Initiation Mechanisms , and Biochemistry Cite this: Biochemistry 2025, 64, 5, 1099–1108 https://doi.org/10.1021/acs.biochem.4c00851 Copyright © 2025 American Chemical Society Request reuse permissions Cite Share Jump to Justin Pi Simpson Joseph* Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Binding Assay:Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. Cloning:Article Title: Supporting Information for High-Throughput Chemical Probing of Full-Length Protein-Protein Interactions Article Snippet: TCO-PEG4-NHS was purchased from Click Chemistry Tools and used as received. .. Horseradish peroxidase (HRP), streptavidin-coated 384-well plates (white, high binding capacity; cat #15505), and SuperSignal West Pico Chemiluminescent substrate kit were purchased from Pierce. eIF4GI-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q04637.4). eIF4EBP1-HaloTag human ORF in pFN21A was purchased from Promega (protein accession ID Q13541.3). pFN29K His6HaloTag T7 Flexi vector and the Flexi System cloning kit were purchased from Promega. FLAG-tag:Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the Clone Assay:Article Title: Prolonging lung cancer response to EGFR inhibition by targeting the selective advantage of resistant cells Article Snippet: Cells were co-transfected with 2 μg of the CRISPR/Cas9 plasmid and 2 μg of ssODN (50 μM) using a Nucleofector II and Amaxa Nucleofector kits (Lonza) according to the manufacturer’s instructions. .. The sequence encoding the Flag tag was cloned between the HindIII and KpnI sites of the |