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Applied Maths pfge band patterns
Pfge Band Patterns, supplied by Applied Maths, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pfge+pattern/pfge+patterns/pm40001431-148-18-30
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pfge band patterns - by Bioz Stars, 2026-11
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Article Title: Clonality, Virulence Genes, and Antimicrobial Resistance of Dairy Ruminants in Mastitic Milk-Associated Staphylococcus aureus in Sicily.
Article Snippet: The digestion was carried out with 3 μL of SmaI restriction enzyme (Fermentas, EU) for 4 h at 25 ◦C, after pre-digestion in the buffer at 25 ◦C for 30 min. DNA fragments were resolved in 1% agarose gel in 0.5X TBE electrophoresis buffer at 14 ◦C using the CHEF-DR III system (Bio-Rad Laboratories, Hercules, CA, USA). .. The runtime was 21 h, with a constant voltage of 6 V/cm, using a linear pulse ramp of 5–40 s. PFGE band patterns were generated by BioNumerics v. 7.5 software (Applied Maths, Sint-Martens-Latem, Belgium) with a tolerance position of 1%. ..

Software:

Article Title: Clonality, Virulence Genes, and Antimicrobial Resistance of Dairy Ruminants in Mastitic Milk-Associated Staphylococcus aureus in Sicily.
Article Snippet: The digestion was carried out with 3 μL of SmaI restriction enzyme (Fermentas, EU) for 4 h at 25 ◦C, after pre-digestion in the buffer at 25 ◦C for 30 min. DNA fragments were resolved in 1% agarose gel in 0.5X TBE electrophoresis buffer at 14 ◦C using the CHEF-DR III system (Bio-Rad Laboratories, Hercules, CA, USA). .. The runtime was 21 h, with a constant voltage of 6 V/cm, using a linear pulse ramp of 5–40 s. PFGE band patterns were generated by BioNumerics v. 7.5 software (Applied Maths, Sint-Martens-Latem, Belgium) with a tolerance position of 1%. ..

Article Title: Delafloxacin activity against Staphylococcus aureus with reduced susceptibility or resistance to methicillin, vancomycin, daptomycin or linezolid.
Article Snippet: Background: Delafloxacin is a recently approved anionic fluoroquinolone antibiotic with broad-spectrum activity against Gram-positive and Gram-negative organisms.. The drug has been approved for patients with acute bacterial skin and skin structure infections including those caused by MRSA.. There are limited data available against MRSA blood isolates (MRSABIs), vancomycin-intermediate strains (VISA), vancomycin-resistant strains (VRSA), daptomycin-non-susceptible strains (DNSSA) and linezolid-resistant Staphylococcus aureus (LRSA).

Article Title: Identification of new sequence types among Enterococcus faecium and Enterococcus faecalis carrying the vanA gene in retail chicken meat.
Article Snippet: In the last years, vancomycin-resistant enterococci (VRE) have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted [1].. In this regard, some studies have documented the worldwide dissemination of particular vancomycin-resistant Enterococcus faecalis clones isolated from healthy and infected humans and other animals [2].. In this study, we report the isolation of new sequence types (STs) of vanA-containing Enterococcus spp. in retail chicken meat in Brazil, highlighting a potential community reservoir of VRE.

Article Title: Shifts in Geographic Distribution and Antimicrobial Resistance during a Prolonged Typhoid Fever Outbreak — Bundibugyo and Kasese Districts, Uganda, 2009–2011
Article Snippet: .. PFGE, which is the current gold standard subtyping technique for Salmonella , was conducted per standard protocols and PFGE patterns were analyzed using BioNumerics software version 5.01 (Applied Maths, Inc., Austin, TX, USA). ..

Article Title: Occurrence of mcr-1 and mcr-2 colistin resistance genes in porcine Escherichia coli isolates (2010–2020) and genomic characterization of mcr-2 -positive E. coli
Article Snippet: .. Xba I-generated PFGE profiles were compared using BioNumerics software (Version 6.6, Applied Maths, Belgium) and cluster analysis of Dice similarity indices based on UPGMA. .. DNA for whole genome sequencing was extracted from E. coli isolates using the DNA Blood & Tissue Kit according to the manufacturer‘s instruction (Qiagen, Hilden, Germany), followed by library preparation, using Nextera XT library (Illumina, San Diego, USA).

Pulsed-Field Gel:

Article Title: Identification of new sequence types among Enterococcus faecium and Enterococcus faecalis carrying the vanA gene in retail chicken meat.
Article Snippet: In the last years, vancomycin-resistant enterococci (VRE) have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted [1].. In this regard, some studies have documented the worldwide dissemination of particular vancomycin-resistant Enterococcus faecalis clones isolated from healthy and infected humans and other animals [2].. In this study, we report the isolation of new sequence types (STs) of vanA-containing Enterococcus spp. in retail chicken meat in Brazil, highlighting a potential community reservoir of VRE.

Sequencing:

Article Title: Identification of new sequence types among Enterococcus faecium and Enterococcus faecalis carrying the vanA gene in retail chicken meat.
Article Snippet: In the last years, vancomycin-resistant enterococci (VRE) have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted [1].. In this regard, some studies have documented the worldwide dissemination of particular vancomycin-resistant Enterococcus faecalis clones isolated from healthy and infected humans and other animals [2].. In this study, we report the isolation of new sequence types (STs) of vanA-containing Enterococcus spp. in retail chicken meat in Brazil, highlighting a potential community reservoir of VRE.

Concentration Assay:

Article Title: Identification of new sequence types among Enterococcus faecium and Enterococcus faecalis carrying the vanA gene in retail chicken meat.
Article Snippet: In the last years, vancomycin-resistant enterococci (VRE) have been increasingly identified in humans and animals, and their potential transmission by contaminated food has been highlighted [1].. In this regard, some studies have documented the worldwide dissemination of particular vancomycin-resistant Enterococcus faecalis clones isolated from healthy and infected humans and other animals [2].. In this study, we report the isolation of new sequence types (STs) of vanA-containing Enterococcus spp. in retail chicken meat in Brazil, highlighting a potential community reservoir of VRE.

other:

Article Title: DGHM Lecture
Article Snippet: When in 1897 Friedrich Loeffler together with Paul Frosch was given the task to identify the causative agent of the devastating ruminant foot-andmouth disease (FMD), he already had made his name as disciple of Robert Koch with the discoveries of the causative agents of malleus (glanders), a bacterial zoonosis, and diphtheria.. Using state-of-the art (filtration) techniques coupled with animal experimentation they concluded that FMD was caused by a new class of infectious agents which were smaller than bacteria (ultrafilterable), were not visible in light microscopes (ultravisible), were capable of replication in the infected organism but were corpuscular entities.. This description was closer to our modern understanding of viruses than any of their contemporaries’.

Article Title: Preliminary evaluation of good sampling locations on a pig carcass for livestock-associated MRSA isolation
Article Snippet: The obtained PFGE profiles were analyzed with Bionumerics version 6.5 (Applied Maths, St.-Martens-Latem, BE) using the unweighted pair group method using averages (UPGMA) with the Dice coefficient (tolerance 1%, tolerance change 1% and optimization 1%).



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PFGE patterns of the NotI-digested V. cholerae O139 strains (clinical isolates BS2, BS3, BS5 BS9, BS19, and BS20; water isolate BW 11) from Odisha and the SG 24 (standard V. cholerae O139) strain, along with dendrogram analysis using BioNumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Analysis showed 3 distinct pulsotypes, suggesting 90% similarity with the standard strain.

Journal: Journal of Clinical Microbiology

Article Title: New Variant of Vibrio cholerae O139 in Odisha, India

doi: 10.1128/JCM.01877-18

Figure Lengend Snippet: PFGE patterns of the NotI-digested V. cholerae O139 strains (clinical isolates BS2, BS3, BS5 BS9, BS19, and BS20; water isolate BW 11) from Odisha and the SG 24 (standard V. cholerae O139) strain, along with dendrogram analysis using BioNumerics software (Applied Maths, Sint-Martens-Latem, Belgium). Analysis showed 3 distinct pulsotypes, suggesting 90% similarity with the standard strain.

Article Snippet: The PFGE result for selected V. cholerae O139 strains, including the water isolate, indicated that there were three different pulsotypes ( ). fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 1 caption a7 Map showing cholera outbreak village, Anandapur block, Keonjhar district. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG 2 caption a7 PFGE patterns of the NotI-digested V. cholerae O139 strains (clinical isolates BS2, BS3, BS5 BS9, BS19, and BS20; water isolate BW 11) from Odisha and the SG 24 (standard V. cholerae O139) strain, along with dendrogram analysis using BioNumerics software (Applied Maths, Sint-Martens-Latem, Belgium).

Techniques: Software