permeabilization solution (Santa Cruz Biotechnology)

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Santa Cruz Biotechnology permeabilization solution
Permeabilization Solution, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/permeabilization+buffer/FCM+Permeabilization+Buffer/pmc09520465-105-2-34
Average 93 stars, based on 43 article reviews

permeabilization solution - by Bioz Stars, 2026-11

93/100 stars

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Blocking Assay:

Article Title: Identification of RC3H1 as antiviral host factor binding to the non-structural protein 1 of Influenza A virus via a 3-stage computational pipeline and cell-based analysis
Article Snippet: .. After permeabilization with 0.2% Triton-X 100 in PBS and blocking with 10% FBS in PBS, the cells were then stained with mouse anti-myc antibody (Santa Cruz Biotechnology) (1:500) or rabbit anti-flag antibody (Millipore) (1:800). .. This was followed by the incubation with goat anti-mouse IgG (H + L) secondary antibody, Alexa FluorTM 568 or anti-rabbit IgG (H + L) secondary antibody, Alexa FluorTM 488 (Invitrogen) diluted 1:500 in blocking solution.

Article Title: Identification of RC3H1 as antiviral host factor binding to the non-structural protein 1 of Influenza A virus via a 3-stage computational pipeline and cell-based analysis.
Article Snippet: .. After permeabilization with 0.2% Triton-X 100 in PBS and blocking with 10% FBS in PBS, the cells were then stained with mouse anti-myc antibody (Santa Cruz Biotechnology) (1:500) or rabbit anti-flag antibody (Millipore) (1:800). .. This was followed by the incubation with goat anti-mouse IgG (H + L) secondary antibody, Alexa FluorTM 568 or anti-rabbit IgG (H + L) secondary antibody, Alexa FluorTM 488 (Invitrogen) diluted 1:500 in blocking solution.

Article Title: PDMS biointerfaces featuring honeycomb-like well microtextures designed for a pro-healing environment
Article Snippet: .. Following fixation, permeabilization and blocking, the cellular monolayer was firstly stained with an anti-vimentin primary antibody (Santa Cruz Biotechnology, dilution 1 : 50) for 2 h, and subsequently incubated for 1 h, in the dark, with an anti-mouse secondary antibody labelled with fluorescein isothiocyanate (FITC) (Santa Cruz Biotechnology, dilution 1 : 200). .. An inverted fluorescence microscope (Olympus IX71, Olympus, Tokyo, Japan) was used to observe the stained cells and the representative fields were captured using the acquisition system cellSense Dimension (Version 4.1).

Article Title: Aetokthonotoxin, the causative agent of vacuolar myelinopathy, uncouples oxidative phosphorylation due to protonophore activity
Article Snippet: .. After treatment, fixation, permeabilization, and blocking, cells were incubated with anti-TOM20 mouse monoclonal antibody (Santa Cruz Biotechnology), washed and incubated with the secondary Alexa Fluor 647 donkey anti-mouse antibody (Invitrogen). .. After treatment, fixation, permeabilization, and blocking, cells were incubated with anti-TOM20 mouse monoclonal antibody (Santa Cruz Biotechnology), washed and incubated with the secondary Alexa Fluor 647 donkey anti-mouse antibody (Invitrogen).

Staining:

Article Title: Identification of RC3H1 as antiviral host factor binding to the non-structural protein 1 of Influenza A virus via a 3-stage computational pipeline and cell-based analysis.
Article Snippet: .. After permeabilization with 0.2% Triton-X 100 in PBS and blocking with 10% FBS in PBS, the cells were then stained with mouse anti-myc antibody (Santa Cruz Biotechnology) (1:500) or rabbit anti-flag antibody (Millipore) (1:800). .. This was followed by the incubation with goat anti-mouse IgG (H + L) secondary antibody, Alexa FluorTM 568 or anti-rabbit IgG (H + L) secondary antibody, Alexa FluorTM 488 (Invitrogen) diluted 1:500 in blocking solution.

Article Title: Large-scale discovery of potent, compact and erythroid specific enhancers for gene therapy vectors.
Article Snippet: To evaluate the differentiation stages during ex vivo erythroid differentiation culture cells were stained with the following antibodies: CD235a-PE (ExBIO) and CD36-APC (ExBIO). .. For the intracellular detection of human γ-globin expression, erythroid cells were fixed in 4% paraformaldehyde followed by permeabilization in 1:1 acetone/H2O, 100% acetone, 1:1 acetone/H2O and stained with anti-human gamma globin (51.7) from Santa Cruz. .. The fraction of enucleated cells was measured with the use of nuclear red (Thermo Fisher Scientific). was excluded using a forward scatter-area and sideward scatter-area gate.

Article Title: Large-scale discovery of potent, compact and erythroid specific enhancers for gene therapy vectors
Article Snippet: To evaluate the differentiation stages during ex vivo erythroid differentiation culture cells were stained with the following antibodies: CD235a-PE (ExBIO) and CD36-APC (ExBIO). .. For the intracellular detection of human γ-globin expression, erythroid cells were fixed in 4% paraformaldehyde followed by permeabilization in 1:1 acetone/H2O, 100% acetone, 1:1 acetone/H2O and stained with anti-human gamma globin (51.7) from Santa Cruz. .. The fraction of enucleated cells was measured with the use of nuclear red (Thermo Fisher Scientific). was excluded using a forward scatter-area and sideward scatter-area gate.

Article Title: Luteolin alleviates olfactory dysfunction in eosinophilic chronic rhinosinusitis through modulation of the TLR4/NF-κB signaling pathway.
Article Snippet: Objectives: Eosinophilic chronic rhinosinusitis (ECRS) is characterized by early, severe olfactory dysfunction and a high recurrence rate, with inadequate treatments.. This article aims to elucidate the potential mechanisms by which luteolin may treat olfactory dysfunction in the context of ECRS.. Methods: Thirty C57 BL/6 mice were randomly assigned to five groups: a control group and four experimental groups (ECRS, ECRS + Luteolin (Low, Medium, High Dose)).

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Article Title: Sustained NF-κB activation allows mutant alveolar stem cells to co-opt a regeneration program for tumor initiation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Dispase (50U/mL) Corning Cat# 354235 Collagenase/dispase solution Sigma Cat# 10269638001 DNase I Sigma Cat# D4527 Gibco HEPES (1M) Invitrogen Cat# 15630080 BMS-345541 Abcam Cat# ab144822 Recombinant murine IL-1b Peprotech Cat# 211-11B Recombinant mouse Dlk-1 R&D Systems Cat# 8545-PR-050 Recombinant mouse osteopontin (Spp1) R&D Systems Cat# 441-OP-050 TransIT -LT1 Transfection Reagent – MIR 2300 GeneFlow Cat# E7-0002 ROCK inhibitor Y-27632 Cambridge Bioscience Cat# SM02-1 Certified Low Melt Agarose Bio-Rad Cat# 1613111 TRIzol Reagent Invitrogen Cat# 15596026 Corning Matrigel Growth Factor Reduced (GFR) Basement Membrane Matrix Corning Cat# 356231 B-27 Supplement (50X), serum free Gibco Cat# 17504044 Recombinant FGF7 Peprotech Cat# 100-19-100 FGF10 Peprotech Cat# 100-26-100 Noggin Peprotech Cat# 250-38 Recombinant EGF Life Technologies Cat# PMG8043 Chiron (CHIR99021) Tocris Bioscience Cat# 4423 Amphotericin B Sigma Cat# A2942 DNase I, Amplification Grade Invitrogen Cat# 18068015 Fast SYBR Green Master Mix Thermo Fisher Scientific Cat# 4385616 Doxycycline Hyclate Sigma Cat# D5207 Critical commercial assays In-Fusion HD cloning kit Clontech Cat# 638951 Alexa Fluor 647 Click-iT Plus EdU Cell Proliferation Kit Invitrogen Cat# C10640 SuperScript IV First-Strand Synthesis System Thermo Fisher Scientific Cat# 18091050 Deposited data scRNA-sequencing of in vivo AT2 cells during homeostasis and oncogenesis This paper GEO: GSE247505 scRNA-sequencing for in vivo AT2-lineage during regeneration Choi et al.11 GEO: GSE145031 Mendeley Data Figure 1 This paper https://doi.org/10.17632/ss6pb96pty.1 Code to extract clonal properties and perform statistical modeling-based analyses This paper https://doi.org/10.5281/zenodo.14625398 Experimental models: Organisms/strains Mouse: Sftpc-CreERT2: B6.129SSftpctm1(cre/ERT2)Blh/J Barkauskas et al.7; The Jackson Laboratory RRID:IMSR_JAX:028054 Mouse: R26R-Confetti: Gt(ROSA) 26Sortm1(CAG-Brainbow2.1)Cle/J Snippert et al.33; The Jackson Laboratory RRID: IMSR_JAX:013731 Mouse: Il1r1-CreERT2: Il1r1tm1(cre/ERT2)Jhle Choi et al.11 MGI:6718882 Mouse: R26R-ZsGreen: B6.Cg-Gt(ROSA) 26Sortm6(CAG-ZsGreen1)Hze/J The Jackson Laboratory RRID:IMSR_JAX:007906 Mouse: Red2KrasG12D: Gt(ROSA) 26Sorem2(CAG-Brainbow2.1,-Kras*)Koo Yum et al.25 MGI:7259836 (Continued on next page) e2 Cell Stem Cell 32, 1–16.e1–e9, March 6, 2025

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