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Structured Review

Merck KGaA 70% percoll solution
70% Percoll Solution, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/percoll+solution/70++percoll+solution/pm40393992-310-4-8
Average 90 stars, based on 1 article reviews
70% percoll solution - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

other:

Article Title: VEGF-A in serum protects against memory impairment in APP/PS1 transgenic mice by blocking neutrophil infiltration.
Article Snippet: Myelin and debris were removed using a modified cold 40% Percoll (Merck Millipore, 17-0891-02) gradient by centrifugation for 30min at 500 × g with no acceleration or braking.

Article Title: CD8 positive T-cells decrease neurogenesis and induce anxiety-like behaviour following hepatitis B vaccination
Article Snippet: Myelin and debris were removed using a modified cold 40% Percoll (Merck Millipore, 17-0891-02) gradient by centrifugation for 30 min at 500 g with no acceleration or braking.

Isolation:

Article Title: Type I interferon drives T cell cytotoxicity by upregulation of interferon regulatory factor 7 in autoimmune kidney diseases in mice.
Article Snippet: For isolating lymphocytes from mouse kidneys, the organs were enzymatically digested with 400 μg/ml collagenase D (Cat# 11088858001; Roche, Mannheim, Germany) and 10 U/ml DNase I (Cat# AMPD1; Sigma-Aldrich, Saint Louis, MO) at 37 °C for 30min, followed by mechanical dissociation using the gentleMACS system (Miltenyi Biotec). .. Then, leukocytes were isolated through density gradient centrifugation using 37% Percoll plus (Cat# GE17-5445-01; Merck Millipore) and a subsequent filtration step through a 30-μm cell strainer. .. Murine cell preparation for flow cytometric analysis After blocking using mice serum, surface labeling of cells was carried out with fluorochrome-conjugated antibodies (CD45 (Cat# 103101; 30- F11; BioLegend), CD3 (Cat# 100311;145-2C11; BioLegend), CD4 (Cat# 100505; RM4-5; BioLegend), CD8 (Cat# 100711; 53-6.7; BioLegend), CD107a (Cat# 121611; 1D4B; BioLegend) along with a fixable dye for dead cells (Cat# L10119; Molecular Probes) to exclude non-viable cells from the analysis.

Gradient Centrifugation:

Article Title: Type I interferon drives T cell cytotoxicity by upregulation of interferon regulatory factor 7 in autoimmune kidney diseases in mice.
Article Snippet: For isolating lymphocytes from mouse kidneys, the organs were enzymatically digested with 400 μg/ml collagenase D (Cat# 11088858001; Roche, Mannheim, Germany) and 10 U/ml DNase I (Cat# AMPD1; Sigma-Aldrich, Saint Louis, MO) at 37 °C for 30min, followed by mechanical dissociation using the gentleMACS system (Miltenyi Biotec). .. Then, leukocytes were isolated through density gradient centrifugation using 37% Percoll plus (Cat# GE17-5445-01; Merck Millipore) and a subsequent filtration step through a 30-μm cell strainer. .. Murine cell preparation for flow cytometric analysis After blocking using mice serum, surface labeling of cells was carried out with fluorochrome-conjugated antibodies (CD45 (Cat# 103101; 30- F11; BioLegend), CD3 (Cat# 100311;145-2C11; BioLegend), CD4 (Cat# 100505; RM4-5; BioLegend), CD8 (Cat# 100711; 53-6.7; BioLegend), CD107a (Cat# 121611; 1D4B; BioLegend) along with a fixable dye for dead cells (Cat# L10119; Molecular Probes) to exclude non-viable cells from the analysis.

Filtration:

Article Title: Type I interferon drives T cell cytotoxicity by upregulation of interferon regulatory factor 7 in autoimmune kidney diseases in mice.
Article Snippet: For isolating lymphocytes from mouse kidneys, the organs were enzymatically digested with 400 μg/ml collagenase D (Cat# 11088858001; Roche, Mannheim, Germany) and 10 U/ml DNase I (Cat# AMPD1; Sigma-Aldrich, Saint Louis, MO) at 37 °C for 30min, followed by mechanical dissociation using the gentleMACS system (Miltenyi Biotec). .. Then, leukocytes were isolated through density gradient centrifugation using 37% Percoll plus (Cat# GE17-5445-01; Merck Millipore) and a subsequent filtration step through a 30-μm cell strainer. .. Murine cell preparation for flow cytometric analysis After blocking using mice serum, surface labeling of cells was carried out with fluorochrome-conjugated antibodies (CD45 (Cat# 103101; 30- F11; BioLegend), CD3 (Cat# 100311;145-2C11; BioLegend), CD4 (Cat# 100505; RM4-5; BioLegend), CD8 (Cat# 100711; 53-6.7; BioLegend), CD107a (Cat# 121611; 1D4B; BioLegend) along with a fixable dye for dead cells (Cat# L10119; Molecular Probes) to exclude non-viable cells from the analysis.



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