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ultrascript cdna synthesis kit  (PCR Biosystems Ltd)


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    Structured Review

    PCR Biosystems Ltd ultrascript cdna synthesis kit
    Ultrascript Cdna Synthesis Kit, supplied by PCR Biosystems Ltd, used in various techniques. Bioz Stars score: 95/100, based on 374 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcr+kit/UltraScript+cDNA+Synthesis+Kit/pmc13019503-37-0-5
    Average 95 stars, based on 374 article reviews
    ultrascript cdna synthesis kit - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Control:

    Article Title: Sex-dependent involvement of lateral septum astrocytes in social fear: Role of oxytocin receptor signaling
    Article Snippet: .. After primer annealing (2 min at 70 °C), 1 μl of each reaction mix was added to the -RT control tubes, and 1 μl of UltraScript 2.0 enzyme (UltraScript 2.0 cDNA Synthesis Kit, PCR Biosystem Inc., USA) was added to the remaining samples. .. All samples were incubated at 25 °C, 50 °C, 80 °C, and 95 °C for 10 min each using a Mastercycler nexus X2 (Eppendorf, Wesseling-Berzdorf, Germany).

    cDNA Synthesis:

    Article Title: Sex-dependent involvement of lateral septum astrocytes in social fear: Role of oxytocin receptor signaling
    Article Snippet: .. After primer annealing (2 min at 70 °C), 1 μl of each reaction mix was added to the -RT control tubes, and 1 μl of UltraScript 2.0 enzyme (UltraScript 2.0 cDNA Synthesis Kit, PCR Biosystem Inc., USA) was added to the remaining samples. .. All samples were incubated at 25 °C, 50 °C, 80 °C, and 95 °C for 10 min each using a Mastercycler nexus X2 (Eppendorf, Wesseling-Berzdorf, Germany).

    Article Title: Ex vivo culture of hematopoietic stem and progenitor cells with platelet lysate: Investigating proliferation and erythroid-megakaryocytic lineage effects.
    Article Snippet: Hematopoietic stem and progenitor cells (HSPCs) are valuable for therapies and research due to their selfrenewal and differentiation abilities.. The ex vivo culture of these cells necessitates conditions that maintain their unique properties and provide a niche-like environment.. Human platelet lysate (HPL), a growth factor-rich substance used in stem cell studies, influences various biological pathways.

    Article Title: Ethylene biosynthesis in guard cells, not mesophyll, predominantly drives stomatal conductance responses to CO 2
    Article Snippet: Total RNA was extracted using the plant total RNA purification kit (Norgen Biotek Corp, Canada) following the manufacturer’s instructions. .. First-strand cDNA was synthesized from ⁓400 ng of total RNA for real-time PCR analysis using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems, UK). .. Real-time PCR was performed using the Applied BiosystemsTM StepOneTM Real-Time PCR System (Applied Biosystems) with SYBRTM Green PCR Master Mix (Applied Biosystems) in the amplification mixture according to the manufacturer’s protocols.

    Article Title: A microbial community that alters mitochondrial morphology and age-related motor function in C. elegans
    Article Snippet: .. Total RNA was extracted using the Direct-zol RNA Mini Prep Kit (Zymogen), using the protocol provided. cDNA was synthesised from extracted RNA diluted to 200 ng/μL in nuclease free water (Promega) using the Ultrascript cDNA Synthesis Kit (PCRBIOSYSTEMS) as per the protocol provided. qPCR was performed using 2× SyGreen Mix Lo-ROX (PCR BIOSYSTEMS) and 500 nM of primers with QuantStudio3 (Applied Biosystems). ..

    Article Title: Evolution of circulating respiratory syncytial virus lineages before and after the COVID-19 pandemic, 2017-2024, Taiwan.
    Article Snippet: RNA extraction and sequencing Total viral RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. Reverse transcription was performed using the UltraScript 2.0 cDNA Synthesis Kit (PCR Biosystems, Wayne, PA). .. The G gene was amplified using PCRBIO Ultra Mix Red (PCR Biosystems, UK) in 25 μL reaction mixtures containing 40 ng of cDNA, 10 μM of each primer, and 2× Ultra Mix Red.

    Article Title: Sex-specific division of labor in termites is linked to differential brain architecture and foraging gene expression
    Article Snippet: Illustra GFX PCR DNA and Gel Band Purification Kit , Cytiva , Cat#28903470. .. Ultrascript cDNA Synthesis Kit , PCR Biosystems , PB30.11–10. .. qPCRBIO SyGreen Mix Separate-Rox , PCR Biosystems , PB20.14–05.

    Article Title: Pluripotent stem cell-derived chimeric antigen receptor-natural killer cells targeting epidermal growth factor receptor 2 for cancer immunotherapy
    Article Snippet: The cells were then incubated for another 48 h. Three days later, successfully transduced hPSCs were selected using 1 μg/ml puromycin (Sigma-Aldrich). .. Total RNA was extracted from cultured cells using an RNeasy Mini Kit (Qiagen). cDNA was synthesized from RNA using a cDNA synthesis kit (PCR Biosystems, London, UK). .. Reverse transcription (RT)-qPCR was then performed with gene-specific primers (Macrogen, Seoul, Republic of Korea), Power SYBR Green PCR Master Mix, and the VIIA 7 Real-Time PCR System (Applied Biosystems).

    Article Title: Antimicrobial Mode of Action: Combined Essential Oils Extracted from Citrus meyeri, Citrus paradise and Citrus sinensis Leaves
    Article Snippet: Naturally derived compounds are powerful tools for controlling microbial infections.. However, the use of these compounds to control microorganism gene expression and growth highlights a new approach to fighting against microbial infections.. The current study aims to investigate the antimicrobial mode of action of combined essential oils (EOs) from Citrus meyeri, Citrus paradise and Citrus sinensis leaves by examining their effects on biofilm formation, cell membrane permeability, and bacterial lysis-related gene expression.

    Synthesized:

    Article Title: Ex vivo culture of hematopoietic stem and progenitor cells with platelet lysate: Investigating proliferation and erythroid-megakaryocytic lineage effects.
    Article Snippet: Hematopoietic stem and progenitor cells (HSPCs) are valuable for therapies and research due to their selfrenewal and differentiation abilities.. The ex vivo culture of these cells necessitates conditions that maintain their unique properties and provide a niche-like environment.. Human platelet lysate (HPL), a growth factor-rich substance used in stem cell studies, influences various biological pathways.

    Article Title: Ethylene biosynthesis in guard cells, not mesophyll, predominantly drives stomatal conductance responses to CO 2
    Article Snippet: Total RNA was extracted using the plant total RNA purification kit (Norgen Biotek Corp, Canada) following the manufacturer’s instructions. .. First-strand cDNA was synthesized from ⁓400 ng of total RNA for real-time PCR analysis using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems, UK). .. Real-time PCR was performed using the Applied BiosystemsTM StepOneTM Real-Time PCR System (Applied Biosystems) with SYBRTM Green PCR Master Mix (Applied Biosystems) in the amplification mixture according to the manufacturer’s protocols.

    Article Title: Pluripotent stem cell-derived chimeric antigen receptor-natural killer cells targeting epidermal growth factor receptor 2 for cancer immunotherapy
    Article Snippet: The cells were then incubated for another 48 h. Three days later, successfully transduced hPSCs were selected using 1 μg/ml puromycin (Sigma-Aldrich). .. Total RNA was extracted from cultured cells using an RNeasy Mini Kit (Qiagen). cDNA was synthesized from RNA using a cDNA synthesis kit (PCR Biosystems, London, UK). .. Reverse transcription (RT)-qPCR was then performed with gene-specific primers (Macrogen, Seoul, Republic of Korea), Power SYBR Green PCR Master Mix, and the VIIA 7 Real-Time PCR System (Applied Biosystems).

    Real-time Polymerase Chain Reaction:

    Article Title: Ethylene biosynthesis in guard cells, not mesophyll, predominantly drives stomatal conductance responses to CO 2
    Article Snippet: Total RNA was extracted using the plant total RNA purification kit (Norgen Biotek Corp, Canada) following the manufacturer’s instructions. .. First-strand cDNA was synthesized from ⁓400 ng of total RNA for real-time PCR analysis using UltraScriptTM cDNA Synthesis Kit (PCR Biosystems, UK). .. Real-time PCR was performed using the Applied BiosystemsTM StepOneTM Real-Time PCR System (Applied Biosystems) with SYBRTM Green PCR Master Mix (Applied Biosystems) in the amplification mixture according to the manufacturer’s protocols.

    Article Title: A microbial community that alters mitochondrial morphology and age-related motor function in C. elegans
    Article Snippet: .. Total RNA was extracted using the Direct-zol RNA Mini Prep Kit (Zymogen), using the protocol provided. cDNA was synthesised from extracted RNA diluted to 200 ng/μL in nuclease free water (Promega) using the Ultrascript cDNA Synthesis Kit (PCRBIOSYSTEMS) as per the protocol provided. qPCR was performed using 2× SyGreen Mix Lo-ROX (PCR BIOSYSTEMS) and 500 nM of primers with QuantStudio3 (Applied Biosystems). ..

    Reverse Transcription:

    Article Title: Evolution of circulating respiratory syncytial virus lineages before and after the COVID-19 pandemic, 2017-2024, Taiwan.
    Article Snippet: RNA extraction and sequencing Total viral RNA was extracted using the QIAamp Viral RNA Mini Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. .. Reverse transcription was performed using the UltraScript 2.0 cDNA Synthesis Kit (PCR Biosystems, Wayne, PA). .. The G gene was amplified using PCRBIO Ultra Mix Red (PCR Biosystems, UK) in 25 μL reaction mixtures containing 40 ng of cDNA, 10 μM of each primer, and 2× Ultra Mix Red.

    Article Title: Antimicrobial Mode of Action: Combined Essential Oils Extracted from Citrus meyeri, Citrus paradise and Citrus sinensis Leaves
    Article Snippet: Naturally derived compounds are powerful tools for controlling microbial infections.. However, the use of these compounds to control microorganism gene expression and growth highlights a new approach to fighting against microbial infections.. The current study aims to investigate the antimicrobial mode of action of combined essential oils (EOs) from Citrus meyeri, Citrus paradise and Citrus sinensis leaves by examining their effects on biofilm formation, cell membrane permeability, and bacterial lysis-related gene expression.

    Cell Culture:

    Article Title: Pluripotent stem cell-derived chimeric antigen receptor-natural killer cells targeting epidermal growth factor receptor 2 for cancer immunotherapy
    Article Snippet: The cells were then incubated for another 48 h. Three days later, successfully transduced hPSCs were selected using 1 μg/ml puromycin (Sigma-Aldrich). .. Total RNA was extracted from cultured cells using an RNeasy Mini Kit (Qiagen). cDNA was synthesized from RNA using a cDNA synthesis kit (PCR Biosystems, London, UK). .. Reverse transcription (RT)-qPCR was then performed with gene-specific primers (Macrogen, Seoul, Republic of Korea), Power SYBR Green PCR Master Mix, and the VIIA 7 Real-Time PCR System (Applied Biosystems).



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    Evolutionary relationships of Hepatocystis parasites. Sequences of the study are highlighted in bold and coloured according to bat host genus. Bayesian inference analyses based on: A sequence alignment of the apicoplast clpC locus (504 nt), B sequence alignment of the nuclear pat locus (831 nt), C sequence alignment of mitochondrial cox1 (915 nt). No reliable cox1 sequences could be amplified for the Taphozous - Hepatocystis samples with the used <t>PCR</t> protocols. D Haplotype median-joining networks of Hepatocystis parasites of Hipposideros spp. bats: (a) cytb (681 nt, n = 7), (b) cytb (487 nt, n = 19), (c) cox1 (640 nt, n = 11), (d) clpC (484 nt, n = 6), (e) ef2 (529 nt, n = 5)
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    Image Search Results


    Journal: iScience

    Article Title: A method for expressing two proteins at a tunable ratio allows for studying the kinetics of creatine transport by MCT12

    doi: 10.1016/j.isci.2026.117719

    Figure Lengend Snippet:

    Article Snippet: CloneAmp HiFi PCR Premix kit , Takara , #639298.

    Techniques: Cell-Signaling, Virus, Recombinant, Clinical Proteomics, Membrane, Staining, Cloning, Software

    Evolutionary relationships of Hepatocystis parasites. Sequences of the study are highlighted in bold and coloured according to bat host genus. Bayesian inference analyses based on: A sequence alignment of the apicoplast clpC locus (504 nt), B sequence alignment of the nuclear pat locus (831 nt), C sequence alignment of mitochondrial cox1 (915 nt). No reliable cox1 sequences could be amplified for the Taphozous - Hepatocystis samples with the used PCR protocols. D Haplotype median-joining networks of Hepatocystis parasites of Hipposideros spp. bats: (a) cytb (681 nt, n = 7), (b) cytb (487 nt, n = 19), (c) cox1 (640 nt, n = 11), (d) clpC (484 nt, n = 6), (e) ef2 (529 nt, n = 5)

    Journal: Parasites & Vectors

    Article Title: High diversity and novel host associations of bat haemosporidian parasites in Cambodia: insights from a nationwide bat survey and multilocus analyses

    doi: 10.1186/s13071-026-07732-3

    Figure Lengend Snippet: Evolutionary relationships of Hepatocystis parasites. Sequences of the study are highlighted in bold and coloured according to bat host genus. Bayesian inference analyses based on: A sequence alignment of the apicoplast clpC locus (504 nt), B sequence alignment of the nuclear pat locus (831 nt), C sequence alignment of mitochondrial cox1 (915 nt). No reliable cox1 sequences could be amplified for the Taphozous - Hepatocystis samples with the used PCR protocols. D Haplotype median-joining networks of Hepatocystis parasites of Hipposideros spp. bats: (a) cytb (681 nt, n = 7), (b) cytb (487 nt, n = 19), (c) cox1 (640 nt, n = 11), (d) clpC (484 nt, n = 6), (e) ef2 (529 nt, n = 5)

    Article Snippet: All PCRs were carried out using the Taq PCR Master Mix Kit (QIAGEN , 201445) in total PCR volumes of 25 μl (12.5 μl of Taq MasterMix, 1.25 μl of each primer, 2 μl of DNA template).

    Techniques: Parasite, Sequencing, Amplification