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Axis-Shield Diagnostics optiprep density gradient separation solution
Optiprep Density Gradient Separation Solution, supplied by Axis-Shield Diagnostics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optiprep+density-gradient+separation/optiprep+gradient/pmc07290849-66-0-8
Average 90 stars, based on 1 article reviews
optiprep density gradient separation solution - by Bioz Stars, 2026-11
90/100 stars

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Purification:

Article Title: TRAF6 and IRF7 Control HIV Replication in Macrophages
Article Snippet: By this criterion, the cells were >97% monocytes differentiated macrophages (MDMs) as assessed by flow-cytometry evaluation by using EPICS XL (Beckman Coulter, Fullerton, CA) (data not shown). .. Viruses were purified with the OptiPrep velocity gradient method (Axis-Shield PoC, Oslo, Norway). ..

Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.

other:

Article Title: Low Immunogenicity of Neural Progenitor Cells Differentiated from Induced Pluripotent Stem Cells Derived from Less Immunogenic Somatic Cells
Article Snippet: Briefly, OptiPrepTM density gradient solutions (Axis-shield) was added in the peripheral blood sample and centrifuged at 900×g for 30 min. PBMCs were collected from the interphase and washed twice with RPMI 1640 medium (Gibco).

Article Title: Bioaugmentation Strategies for Enhancing Methane Production from Shrimp Processing Waste through Anaerobic Digestion
Article Snippet: Before FISH analysis, a Fermentation 2023, 9, 401 6 of 15 cell extraction procedure was performed to detach and separate cells from substrate and/or inorganic particles [41] using an OptiPrepTM density gradient medium (Axis-Shield PoC As, Oslo, Norway).

Article Title: Calycosin promotes axon growth by inhibiting PTPRS and alleviates spinal cord injury.
Article Snippet: Our former studies have identified the alleviating effect of Calycosin (CA) on spinal cord injury (SCI).. In this study, our purpose is to explore the influence of CA on SCI from the perspective of promoting axon growth.. The SCI animal model was constructed by spinal cord compression, wherein rat primary cortex neuronal isolation was performed, and the axonal growth restriction cell model was established via chondroitin sulfate proteoglycan (CSPG) treatment.

Article Title: Elucidating cellular response to treatment with viral immunotherapies in pediatric high-grade glioma and medulloblastoma
Article Snippet: G207 was grown in Vero cells and purified on OptiPrepTM gradients (AXIS-SHIELD Oslo, Norway) .

Cell Isolation:

Article Title: High-dimensional analysis reveals an immune atlas and novel neutrophil clusters in the lungs of model animals with Actinobacillus pleuropneumoniae- induced pneumonia
Article Snippet: .. After following the steps in mouse cell isolation to obtain single cells, these cells were further centrifuged using OptiPrep separation medium (Axis-Shield, Dundee, UK) to remove dead cells. ..

Magnetic Cell Separation:

Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.

Tissue:

Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.

Centrifugation:

Article Title: Macrophagic and microglial responses after focal traumatic brain injury in the female rat
Article Snippet: .. Tissues underwent enzymatic and mechanical dissociation with the MACS Neural Tissue Dissociation Kit (P) (Miltenyi Biotec, Bergisch Gladbach, Germany), followed by cell purification by centrifugation in an OptiPrep gradient medium (Axis-Shield, Oslo, Norway) to remove myelin and debris and to enrich the tissues for brain-derived macrophages and microglia as described previously [ ]. .. After isolation, cells were fixed with BD Cytofix fixation buffer (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s instructions.



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