ubp310 3 3 2s 2 amino 2 carboxyethyl 5 methyl 2 6 dioxo 3 6 dihydro 1 2h pyrimidinyl methyl 2 thiophenecarboxylic acid (Tocris)
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ubp310 3 3 2s 2 amino 2 carboxyethyl 5 methyl 2 6 dioxo 3 6 dihydro 1 2h pyrimidinyl methyl 2 thiophenecarboxylic acid
Ubp310 3 3 2s 2 Amino 2 Carboxyethyl 5 Methyl 2 6 Dioxo 3 6 Dihydro 1 2h Pyrimidinyl Methyl 2 Thiophenecarboxylic Acid, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+microscope+motic+ba+310/UBP+310/pmc07656261-78-0-6
Average 94 stars, based on 25 article reviews
Ubp310 3 3 2s 2 Amino 2 Carboxyethyl 5 Methyl 2 6 Dioxo 3 6 Dihydro 1 2h Pyrimidinyl Methyl 2 Thiophenecarboxylic Acid, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 25 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/optical+microscope+motic+ba+310/UBP+310/pmc07656261-78-0-6
Average 94 stars, based on 25 article reviews
ubp310 3 3 2s 2 amino 2 carboxyethyl 5 methyl 2 6 dioxo 3 6 dihydro 1 2h pyrimidinyl methyl 2 thiophenecarboxylic acid - by Bioz Stars,
2026-11
94/100 stars
Images
1) Product Images from "Pharmacological antagonism of kainate receptor rescues dysfunction and loss of dopamine neurons in a mouse model of human parkin-induced toxicity"
Article Title: Pharmacological antagonism of kainate receptor rescues dysfunction and loss of dopamine neurons in a mouse model of human parkin-induced toxicity
Journal: Cell Death & Disease
doi: 10.1038/s41419-020-03172-8
Figure Legend Snippet: Representative images showing TH immunoperoxidase labeling in the SNc of the WT and the parkinQ311X mice (6 months of age) treated with either vehicle or UBP310 (20 mg/kg i.p.) for 90 or 135 days. The graph below the picture shows DA neuron quantification performed by stereological count. Data are the means ± SEM of TH-positive neurons: WT treated with vehicle for 90 days 5578 ± 235 n = 8 mice, WT treated with UBP310 for 90 days 5351 ± 162 n = 7 mice, parkinQ311X treated with vehicle for 90 days 4701 ± 117 n = 11 mice, parkinQ311X treated with UBP310 for 90 days 5347 ± 107 n = 12 mice, parkinQ311X mice treated with UBP310 for 135 days 5514 ± 94 n = 9 mice, one-way ANOVA followed by Tukey’s test F = 6.681, WT vehicle 90 days vs. parkinQ311X vehicle 90 days *** p = 0.0007, WT UBP310 90 days vs. parkinQ311X vehicle 90 days * p = 0.0260, parkinQ311X vehicle 90 days vs. parkinQ311X UBP310 90 days ** p = 0.0073, parkinQ311X vehicle 90 days vs. parkinQ311X UBP310 135 days ** p = 0.0012.
Techniques Used: Labeling
Figure Legend Snippet: a Examples of patch-clamp whole-cell recordings in current-clamp mode from an SNc DA neuron in an acute slice of ventral midbrain. The upper traces show membrane potential hyperpolarizing and depolarizing responses to intracellular injection of negative and positive current steps, respectively (±200 pA, 500 ms, lower traces). Note the relatively depolarized resting-membrane potential (−49 mV) and the prominent I h -dependent depolarizing “sag” (arrow) typical of DA neurons. b Cell-attached recordings of spontaneous action potential (AP) firing of SNc DA neurons from WT and parkinQ311X mice at 25 days of age. The dot plot on the right shows the quantification of spontaneous firing frequencies. The mean firing frequency of the SNc DA neurons was significantly higher in parkinQ311X mice compared to WT mice (mean ± SEM WT: 1.75 ± 0.18 Hz, parkinQ311X mice: 2.96 ± 0.23 Hz). Data were obtained from 6 WT mice (16 recorded neurons) and 8 parkinQ311X mice (36 recorded neurons). Unpaired Student t test with Welch correction, *** p = 0.0001, t = 4.148, d f = 47.75. c Summary dot plots showing spontaneous firing frequencies of SNc DA neurons in acute slices of ventral midbrain prepared from WT and parkinQ311X mice at 25 days of age upon extracellular perfusion with UBP310 (5 and 10 µM) and subsequent washout. Mean ± SEM, WT ACSF 2.3 ± 0.46 Hz, WT treated with UBP310 5 µM 2.03 ± 0.37 Hz, WT treated with UBP310 10 µM 1.85 ± 0.32 Hz, WT washout 1.96 ± 0.31 Hz; * p = 0.02, F = 7.83, Friedman repeated-measure ANOVA on ranks. Q311X ACSF 3.4 ± 0.5 Hz, Q311X treated with UBP310 5 µM 2.61 ± 0.36 Hz, Q311X treated with UBP310 10 µM 2.46 ± 0.41 Hz, Q311X washout 3.0 ± 0.55 Hz; one-way repeated-measure ANOVA Holm–Sidak test ** p = 0.009, F = 11.87 (7 recorded neurons for each condition).
Techniques Used: Patch Clamp, Membrane, Injection
Figure Legend Snippet: a Representative Western blots showing GluK2 immunostaining from total brain lysates obtained from WT or parkinQ311X mice treated with 20 mg/kg UBP310 i.p. for either 4 or 8 h. The graphs show the means ± SEM from densitometer quantifications (WT—white bars, Q311X—gray bars). UBP310 treatment induced a decrease in GluK2 levels. WT mice, NT 1.00 ± 0.02, 4 h 0.77 ± 0.03, 8 h 0.71 ± 0.08, 4 h vs. NT * p = 0.047, 8 h vs. NT * p = 0.0011, F = 7.276, one-way ANOVA with Bonferroni’s test. ParkinQ311X mice, NT 1.00 ± 0.05, 4 h 0.92 ± 0.04, 8 h 0.73 ± 0.02, 8 h vs. NT ** p = 0.0013, F = 13.36, one-way ANOVA with Bonferroni’s test. UBP310 did not change the levels of AMPA receptor subunits GluA1 and GluA2. b Representative confocal images showing TH (red) and GluK2 (green) double immunofluorescence in the SNc of WT or parkinQ311X mice treated with either vehicle or 20 mg/kg UBP310 i.p. for 8 h. Images were acquired using a Leica TCS SP2 confocal microscope, objective 40× (bar, 50 µm). The histograms show the means ± SEM from fluorescence-intensity quantifications. Twelve sections per genotype/treatment were examined derived from n = 3 WT mice treated with vehicle, n = 3 WT mice treated with UBP310, n = 3 parkinQ311X mice treated with vehicle, and n = 3 parkinQ311X mice treated with UBP310. GluK2/TH: WT vehicle 1.00 ± 0.08 n = 95 neurons, WT UBP310 0.7 ± 0.09 n = 91 neurons, parkinQ311X vehicle 1.67 ± 0.08 n = 102 neurons, parkinQ311X UBP310 1.25 ± 0.07 n = 100 neurons, WT vehicle vs. WT UBP310 * p = 0.039, Q311X vehicle vs. Q311X UBP310 p = 0.0006, WT vehicle vs. Q311X vehicle p = 0.00009, F = 27.95, one-way ANOVA followed by Tukey’s test.
Techniques Used: Western Blot, Immunostaining, Immunofluorescence, Microscopy, Fluorescence, Derivative Assay
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