omniscript (Promega)
90
Structured Review
Promega
omniscript
Omniscript, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/omniscript+kit/omniscript+rt+kit/pm34194479-128-33-28
Average 90 stars, based on 1 article reviews
Omniscript, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/omniscript+kit/omniscript+rt+kit/pm34194479-128-33-28
Average 90 stars, based on 1 article reviews
omniscript - by Bioz Stars,
2026-09
90/100 stars
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Reverse Transcription:Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Article Title: Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR Article Snippet: RNA was extracted from ~1 × 10 logarithmic phase cells using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. A two-step RT-PCR reaction was performed by first transcribing 0.25 μg of RNA using an Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Article Title: Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro . Article Snippet: Total RNA was isolated from cells from each group using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treatment of RNA was performed using DNase (Takara Biotechnology, Co., Ltd., Dalian, China). .. Reverse transcription was performed using an Article Title: Evidence of distinct RELN and TGFB1 genetic associations in familial and non-familial otosclerosis in a British population Article Snippet: RNA was also extracted from homogenised human MG-63 cells, a human osteosarcoma cell line using the RNeasy mini kit (Qiagen) in accordance with the manufacturer’s protocol. .. RNA was reverse transcribed into cDNA by Article Title: Complete destruction of deep-tissue buried tumors via combination of gene silencing and gold nanoechinus-mediated photodynamic therapy. Article Snippet: Cancer is one of the major diseases leading to human deaths.. Complete destruction of deep tissue-buried tumors using non-invasive therapies is a grand challenge in clinical cancer treatments.. Many therapeutic modalities were developed to tackle this problem, but only partial tumor suppression or delay growths were usually achieved. Purification:Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Polymerase Chain Reaction:Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Article Title: Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR Article Snippet: RNA was extracted from ~1 × 10 logarithmic phase cells using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. A two-step RT-PCR reaction was performed by first transcribing 0.25 μg of RNA using an Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Spectrophotometry:Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Article Title: Calcium-Alginate Hydrogel-Encapsulated Fibroblasts Provide Sustained Release of Vascular Endothelial Growth Factor Article Snippet: Semiquantitative reverse transcription-polymerase chain reaction RNeasy minikits (Qiagen) and DNase kits (Qiagen) were used to extract mRNA. mRNA quality and quantity were evaluated and quantified using a spectrophotometer (BioPhotometer) and by electrophoresis using a 1% w/v agarose gel with ethidium bromide (Promega). .. Reverse Transcription Polymerase Chain Reaction:Article Title: Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR Article Snippet: RNA was extracted from ~1 × 10 logarithmic phase cells using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. A two-step RT-PCR reaction was performed by first transcribing 0.25 μg of RNA using an Amplification:Article Title: Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR Article Snippet: RNA was extracted from ~1 × 10 logarithmic phase cells using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. A two-step RT-PCR reaction was performed by first transcribing 0.25 μg of RNA using an Sequencing:Article Title: Dynamic regulation of the Trypanosoma brucei transferrin receptor in response to iron starvation is mediated via the 3’UTR Article Snippet: RNA was extracted from ~1 × 10 logarithmic phase cells using the RNeasy plus kit (Qiagen) according to the manufacturer’s instructions. .. A two-step RT-PCR reaction was performed by first transcribing 0.25 μg of RNA using an Real-time Polymerase Chain Reaction:Article Title: Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro . Article Snippet: Total RNA was isolated from cells from each group using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treatment of RNA was performed using DNase (Takara Biotechnology, Co., Ltd., Dalian, China). .. Reverse transcription was performed using an Gene Expression:Article Title: Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro . Article Snippet: Total RNA was isolated from cells from each group using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treatment of RNA was performed using DNase (Takara Biotechnology, Co., Ltd., Dalian, China). .. Reverse transcription was performed using an SYBR Green Assay:Article Title: Isoquercetin activates the ERK1/2-Nrf2 pathway and protects against cerebral ischemia-reperfusion injury in vivo and in vitro . Article Snippet: Total RNA was isolated from cells from each group using TRIzol reagent (Invitrogen; Thermo Fisher Scientific, Inc.) and treatment of RNA was performed using DNase (Takara Biotechnology, Co., Ltd., Dalian, China). .. Reverse transcription was performed using an Isolation:Article Title: The RNA-Binding Proteins SRP14 and HMGB3 Control HIV-1 Tat mRNA Processing and Translation During HIV-1 Latency. Article Snippet: After 2 days, cells were collected and stained for CD25 (PE-Cy7, clone 2A3, BD) and HLA-DR (V605, clone L243 BioLegend) activation markers in comparison to PHA stimulated (10 μg/mL) cells. .. Culture supernatant was also harvested and viral RNA was isolated using QIAamp Viral RNA Mini Kit (Qiagen) following the manufacturer’s protocol. vRNA was DNase treated (2 U/μg RNA, Promega) and reversed transcribed using |