Incubation:Article Title: The adverse effect of a hypercaloric high-fat diet feeding on hypothalamic cellular energy homeostasis is attenuated by a hypoglycemiant.
Article Snippet: Laboratorio de Neuroendocrinología y Metabolismo, Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile School of Nutrition and Dietetics, Faculty of Rehabilitation and Quality of Life Sciences, Universidad San Sebastián, Puerto Montt, Chile Laboratorio de Nutrición Funcional, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, Chile Laboratorio de Biología Celular del Cáncer, Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile Laboratory of Neurobiology of Aging, Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile Laboratorio de Enfermedades Crónicas, Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile
Article Title: Impact of Adipokine Expression on Tumor Characteristics and Survival Outcomes in Patients with Renal Cell Carcinoma
Article Snippet: The 4 μm thick sections of formalin-fixed and paraffin-embedded TMA tissues were deparaffinized in Bond Devax solution (Leica Microsystems) at 72 degrees. .. A mouse monoclonal anti-adiponectin antibody (ab22554, 1:100; Abcam, Cambridge, UK) and a mouse monoclonal resistin antibody [(C-10): sc-376336, 1:100, Santa Cruz Biotechnology, Inc., Dallas, TX, USA] were incubated in ER1 for 20 min, a mouse monoclonal anti-leptin-R antibody [(B-3): sc8391, 1:200, Santa Cruz Biotechnology, Inc., Dallas, USA] and a mouse monoclonal anti-leptin antibody [(F-3), sc48408, 1:200, Santa Cruz Biotechnology, Inc., Dallas, USA] were incubated in ER2 for 10 min at 100 degrees for pre-treatment. ..
Gentle:Article Title: The adverse effect of a hypercaloric high-fat diet feeding on hypothalamic cellular energy homeostasis is attenuated by a hypoglycemiant.
Article Snippet: Laboratorio de Neuroendocrinología y Metabolismo, Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile School of Nutrition and Dietetics, Faculty of Rehabilitation and Quality of Life Sciences, Universidad San Sebastián, Puerto Montt, Chile Laboratorio de Nutrición Funcional, Instituto de Nutrición y Tecnología de los Alimentos (INTA), Universidad de Chile, Santiago, Chile Laboratorio de Biología Celular del Cáncer, Centro de Biología Celular y Biomedicina (CEBICEM), Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile Laboratory of Neurobiology of Aging, Centro Científico y Tecnológico de Excelencia Ciencia & Vida, Fundación Ciencia & Vida, Santiago, Chile Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile Laboratorio de Enfermedades Crónicas, Facultad de Ciencias, Universidad San Sebastián, Santiago, Chile
Immunofluorescence:
Staining:
Chromatin Immunoprecipitation:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier.
Flow Cytometry:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier.
Magnetic Resonance Imaging:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier.
Biomarker Discovery:Article Title: Regenerating aged bone marrow via a nitric oxide nanopump
Article Snippet: .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier. .. Materials & experimental systems n/a Involved in the study Antibodies Eukaryotic cell lines Palaeontology and archaeology Animals and other organisms Clinical data Dual use research of concern Plants Methods n/a Involved in the study ChIP-seq Flow cytometry MRI-based neuroimaging Antibodies Antibodies used Validation Eukaryotic cell lines Policy information about cell lines and Sex and Gender in Research Cell line source(s) Authentication anti-LepR-biotin antibody(BAF497, R&D Systems), anti-LepR-FITC antibody (sc-8391 FITC, Santa Cruz Biotechnology), anti-OCN antibody(PA5-78870, Thermo Fisher Scientific), anti-ASL antibody(ab97370, Abcam), anti-P21 antibody(ab188224, Abcam), anti-!H2AX antibody(ab81299, Abcam),anti-IL-6 (ab290735,Abcam), anti-PERILIPIN antibody(ab316115, Abcam), anti-CD31 antibody (ab222783, Abcam), anti-LYVE1 antibody(ab14917, Abcam), anti-PERIPHERIN antibody(ab246502, Abcam), donkey anti-goat FITC antibody(ab6881, Abcam), goat anti-rabbit FITC antibody(ab6717, Abcam),anti-Lineage Cocktail Isotype Ctrl-FITC antibody(133302, BioLegend), anti-Sca-1-APC antibody(108112, BioLegend), anti-c-Kit-PE/Cyanine7 antibody(105814, BioLegend), anti-CD48-PerCP/ Cyanine5.5 antibody(103422, BioLegend), and anti-CD150-PE antibody(162606, BioLegend) All antibodies were verified by the supplier.
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Santa Cruz Biotechnology is a verified supplier 