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negative control oligonucleotide sequences lv nc  (Genechem)

 
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    Structured Review

    Genechem negative control oligonucleotide sequences lv nc
    Negative Control Oligonucleotide Sequences Lv Nc, supplied by Genechem, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/negative+control+sequence/control+negative/pmc12932789-107-5-13
    Average 86 stars, based on 1 article reviews
    negative control oligonucleotide sequences lv nc - by Bioz Stars, 2026-11
    86/100 stars

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    Related Articles

    Control:

    Article Title: Adipose extracellular vesicles carrying miR-210-3p drive macrophage inflammation and nicotine-induced atherosclerosis
    Article Snippet: AAV9-mmu-miR-210-3p inhibition virus , GeneChem , Serotype: AAV9; Vector: GV481; U6-MCS-CAG-mCherry; Titer: 1 × 10ˆ12 vg/mL. .. AAV9-negative control virus , GeneChem , Serotype: AAV9; Vector: GV481; U6-MCS-CAG-mCherry; Titer: 1 × 10ˆ12 vg/mL. .. Lentiviral KLF7 overexpression virus , GeneChem , Vector: GV737; CMV-MCS-EF1a-mCherry-T2A-puromycin.

    Virus:

    Article Title: Adipose extracellular vesicles carrying miR-210-3p drive macrophage inflammation and nicotine-induced atherosclerosis
    Article Snippet: AAV9-mmu-miR-210-3p inhibition virus , GeneChem , Serotype: AAV9; Vector: GV481; U6-MCS-CAG-mCherry; Titer: 1 × 10ˆ12 vg/mL. .. AAV9-negative control virus , GeneChem , Serotype: AAV9; Vector: GV481; U6-MCS-CAG-mCherry; Titer: 1 × 10ˆ12 vg/mL. .. Lentiviral KLF7 overexpression virus , GeneChem , Vector: GV737; CMV-MCS-EF1a-mCherry-T2A-puromycin.

    Article Title: Targeting the macrophage mechanosensing regulator CKAP4 accelerates the inflammatory-to-proliferative transition in wound healing.
    Article Snippet: Cat# T002407 Mice: db/m GemPharmatech Co.,Ltd. .. Cat# T002406 Oligonucleotides Primers for RT-qPCR, see Table S1 Sangon biotech N/A Primers for genotyping, see Table S1 Sangon biotech N/A siRNA HanBio Technology N/A Recombinant DNA pCMV-CKAP4-3×HA Miaoling bio N/A pCMV-CKAP4Δ1-86-3×HA Miaoling bio N/A pCMV-CKAP4Δ125-194-3×HA Miaoling bio N/A pCMV-CKAP4Δ236-439-3×HA Miaoling bio N/A pCMV-CKAP4Δ507-575-3×HA Miaoling bio N/A Bacterial and virus strains AAV9-F4/80-shCkap4-ZsGreen HanBio Technology N/A AAV9-F4/80-ZsGreen HanBio Technology N/A CKAP4-OE lentivirus GeneChem N/A Lentiviral negative control GeneChem N/A Software and algorithms FlowJo_V10 BD Biosciences https://www.flowjo.com GraphPad Prism 9.5.0 GraphPad Software https://www.graphpad.com ImageJ ImageJ https://imagej.nih.gov/ij AlphaFold3 DeepMind & Isomorphic Labs https://alphafoldserver.com PyMOL Schrödinger, LLC https://pymol.org RStudio Posit Software, PBC https://posit.co/rstudio CellProfiler Broad Institute of MIT and Harvard https://cellprofiler.org/ Cellpose-SAM Cellpose developers (Mouseland) https://www.cellpose.org Other Transparent wound dressing 3M Cat# 3589 Instant krazy glue Columbus Cat# 744-3514 AFM probe Bruker Cat# MLCT-SPH-5UM-DC 20 Cell Reports 45, 117395, June 23, 2026 Primary cell cultures and cell line Primary murine bone marrow-derived macrophages (BMDMs) were isolated from male WT and Ckap4− /− mice. ..

    Article Title: Hyaluronic acid and folic acid-modified mesoporous silica nanoparticles delivering sulforaphane suppress NSCLC via SPI1/miR-616-5p axis
    Article Snippet: Lentiviral SPI1 overexpression virus , GeneChem , N/A. .. Lentiviral negative control virus , GeneChem , N/A. .. Lentiviral shRNA targeting human SPI1 , GeneChem , N/A.

    Plasmid Preparation:

    Article Title: Adipose extracellular vesicles carrying miR-210-3p drive macrophage inflammation and nicotine-induced atherosclerosis
    Article Snippet: AAV9-mmu-miR-210-3p inhibition virus , GeneChem , Serotype: AAV9; Vector: GV481; U6-MCS-CAG-mCherry; Titer: 1 × 10ˆ12 vg/mL. .. AAV9-negative control virus , GeneChem , Serotype: AAV9; Vector: GV481; U6-MCS-CAG-mCherry; Titer: 1 × 10ˆ12 vg/mL. .. Lentiviral KLF7 overexpression virus , GeneChem , Vector: GV737; CMV-MCS-EF1a-mCherry-T2A-puromycin.

    Recombinant:

    Article Title: Targeting the macrophage mechanosensing regulator CKAP4 accelerates the inflammatory-to-proliferative transition in wound healing.
    Article Snippet: Cat# T002407 Mice: db/m GemPharmatech Co.,Ltd. .. Cat# T002406 Oligonucleotides Primers for RT-qPCR, see Table S1 Sangon biotech N/A Primers for genotyping, see Table S1 Sangon biotech N/A siRNA HanBio Technology N/A Recombinant DNA pCMV-CKAP4-3×HA Miaoling bio N/A pCMV-CKAP4Δ1-86-3×HA Miaoling bio N/A pCMV-CKAP4Δ125-194-3×HA Miaoling bio N/A pCMV-CKAP4Δ236-439-3×HA Miaoling bio N/A pCMV-CKAP4Δ507-575-3×HA Miaoling bio N/A Bacterial and virus strains AAV9-F4/80-shCkap4-ZsGreen HanBio Technology N/A AAV9-F4/80-ZsGreen HanBio Technology N/A CKAP4-OE lentivirus GeneChem N/A Lentiviral negative control GeneChem N/A Software and algorithms FlowJo_V10 BD Biosciences https://www.flowjo.com GraphPad Prism 9.5.0 GraphPad Software https://www.graphpad.com ImageJ ImageJ https://imagej.nih.gov/ij AlphaFold3 DeepMind & Isomorphic Labs https://alphafoldserver.com PyMOL Schrödinger, LLC https://pymol.org RStudio Posit Software, PBC https://posit.co/rstudio CellProfiler Broad Institute of MIT and Harvard https://cellprofiler.org/ Cellpose-SAM Cellpose developers (Mouseland) https://www.cellpose.org Other Transparent wound dressing 3M Cat# 3589 Instant krazy glue Columbus Cat# 744-3514 AFM probe Bruker Cat# MLCT-SPH-5UM-DC 20 Cell Reports 45, 117395, June 23, 2026 Primary cell cultures and cell line Primary murine bone marrow-derived macrophages (BMDMs) were isolated from male WT and Ckap4− /− mice. ..

    Negative Control:

    Article Title: Targeting the macrophage mechanosensing regulator CKAP4 accelerates the inflammatory-to-proliferative transition in wound healing.
    Article Snippet: Cat# T002407 Mice: db/m GemPharmatech Co.,Ltd. .. Cat# T002406 Oligonucleotides Primers for RT-qPCR, see Table S1 Sangon biotech N/A Primers for genotyping, see Table S1 Sangon biotech N/A siRNA HanBio Technology N/A Recombinant DNA pCMV-CKAP4-3×HA Miaoling bio N/A pCMV-CKAP4Δ1-86-3×HA Miaoling bio N/A pCMV-CKAP4Δ125-194-3×HA Miaoling bio N/A pCMV-CKAP4Δ236-439-3×HA Miaoling bio N/A pCMV-CKAP4Δ507-575-3×HA Miaoling bio N/A Bacterial and virus strains AAV9-F4/80-shCkap4-ZsGreen HanBio Technology N/A AAV9-F4/80-ZsGreen HanBio Technology N/A CKAP4-OE lentivirus GeneChem N/A Lentiviral negative control GeneChem N/A Software and algorithms FlowJo_V10 BD Biosciences https://www.flowjo.com GraphPad Prism 9.5.0 GraphPad Software https://www.graphpad.com ImageJ ImageJ https://imagej.nih.gov/ij AlphaFold3 DeepMind & Isomorphic Labs https://alphafoldserver.com PyMOL Schrödinger, LLC https://pymol.org RStudio Posit Software, PBC https://posit.co/rstudio CellProfiler Broad Institute of MIT and Harvard https://cellprofiler.org/ Cellpose-SAM Cellpose developers (Mouseland) https://www.cellpose.org Other Transparent wound dressing 3M Cat# 3589 Instant krazy glue Columbus Cat# 744-3514 AFM probe Bruker Cat# MLCT-SPH-5UM-DC 20 Cell Reports 45, 117395, June 23, 2026 Primary cell cultures and cell line Primary murine bone marrow-derived macrophages (BMDMs) were isolated from male WT and Ckap4− /− mice. ..

    Article Title: YARS2 mediates PARPi resistance in pancreatic cancer by regulating the MYC-E2F axis.
    Article Snippet: Background: Pancreatic ductal adenocarcinoma (PDAC) resistance to PARP inhibitors (PARPi) remains poorly understood, the mechanism of resistance remain poorly understood.. Material and Methods: We integrated multi-omics data from The Cancer Genome Atlas-Pancreatic Adenocarcinoma (TCGA-PAAD) datasets, Cancer Cell Line Encyclopedia (CCLE), and the Genomics of Drug Sensitivity in Cancer (GDSC) performed weighted gene co-expression network analysis (WGCNA) to identify hub genes linked to PARPi resistance.. GSE86394 from the Gene Expression Omnibus (GEO) database was utilized to differential expressed genes (DEGs) and performed functional enrichment analyses.

    Article Title: Intratumoral microbiota promote pancreatic cancer progression via NaAc-mediated activation of the GPR43/AMPK/HADH pathway.
    Article Snippet: .. Short hairpin RNA (shRNA) targeting 3-hydroxyacyl-CoA dehydrogenase (HADH) and negative control shRNA (sh-NC) were generated by GeneChem (Shanghai). .. Small interfering RNA (siRNA) targeting G protein-coupled receptor 43 (GPR43) was constructed by GenePharma (Shanghai).

    Article Title: FTO Deficiency Inhibits Vascular Endothelial Cell Apoptosis and Improved Mitophagy in Obesity-Related Hypertension by Enhancing NDRG1 m6A Methylation.
    Article Snippet: Vascular endothelial dysfunction contributes to obesity-related hypertension, yet its mechanisms remain unclear.. This study examined the role of N6-methyladenosine (m6A) in this process using in vivo and in vitro models.. C57BL/6J mice fed a highfat diet (HFD) developed obesity-related hypertension, while oxidized low-density lipoprotein (ox-LDL)-treated human aortic endothelial cells (HAECs) simulated endothelial injury.

    Article Title: Hyaluronic acid and folic acid-modified mesoporous silica nanoparticles delivering sulforaphane suppress NSCLC via SPI1/miR-616-5p axis
    Article Snippet: Lentiviral SPI1 overexpression virus , GeneChem , N/A. .. Lentiviral negative control virus , GeneChem , N/A. .. Lentiviral shRNA targeting human SPI1 , GeneChem , N/A.

    Article Title: Identification and validation of programmed cell death-related genes in osteosarcoma: inhibiting CIB1 as a promising therapeutic strategy.
    Article Snippet: .. Cells were transduced with lentiviral particles carrying either negative control shRNA (NC-shRNA) or CIB1-targeting shRNA (GeneChem Corporation, Shanghai, China). ..

    Software:

    Article Title: Targeting the macrophage mechanosensing regulator CKAP4 accelerates the inflammatory-to-proliferative transition in wound healing.
    Article Snippet: Cat# T002407 Mice: db/m GemPharmatech Co.,Ltd. .. Cat# T002406 Oligonucleotides Primers for RT-qPCR, see Table S1 Sangon biotech N/A Primers for genotyping, see Table S1 Sangon biotech N/A siRNA HanBio Technology N/A Recombinant DNA pCMV-CKAP4-3×HA Miaoling bio N/A pCMV-CKAP4Δ1-86-3×HA Miaoling bio N/A pCMV-CKAP4Δ125-194-3×HA Miaoling bio N/A pCMV-CKAP4Δ236-439-3×HA Miaoling bio N/A pCMV-CKAP4Δ507-575-3×HA Miaoling bio N/A Bacterial and virus strains AAV9-F4/80-shCkap4-ZsGreen HanBio Technology N/A AAV9-F4/80-ZsGreen HanBio Technology N/A CKAP4-OE lentivirus GeneChem N/A Lentiviral negative control GeneChem N/A Software and algorithms FlowJo_V10 BD Biosciences https://www.flowjo.com GraphPad Prism 9.5.0 GraphPad Software https://www.graphpad.com ImageJ ImageJ https://imagej.nih.gov/ij AlphaFold3 DeepMind & Isomorphic Labs https://alphafoldserver.com PyMOL Schrödinger, LLC https://pymol.org RStudio Posit Software, PBC https://posit.co/rstudio CellProfiler Broad Institute of MIT and Harvard https://cellprofiler.org/ Cellpose-SAM Cellpose developers (Mouseland) https://www.cellpose.org Other Transparent wound dressing 3M Cat# 3589 Instant krazy glue Columbus Cat# 744-3514 AFM probe Bruker Cat# MLCT-SPH-5UM-DC 20 Cell Reports 45, 117395, June 23, 2026 Primary cell cultures and cell line Primary murine bone marrow-derived macrophages (BMDMs) were isolated from male WT and Ckap4− /− mice. ..

    Isolation:

    Article Title: Targeting the macrophage mechanosensing regulator CKAP4 accelerates the inflammatory-to-proliferative transition in wound healing.
    Article Snippet: Cat# T002407 Mice: db/m GemPharmatech Co.,Ltd. .. Cat# T002406 Oligonucleotides Primers for RT-qPCR, see Table S1 Sangon biotech N/A Primers for genotyping, see Table S1 Sangon biotech N/A siRNA HanBio Technology N/A Recombinant DNA pCMV-CKAP4-3×HA Miaoling bio N/A pCMV-CKAP4Δ1-86-3×HA Miaoling bio N/A pCMV-CKAP4Δ125-194-3×HA Miaoling bio N/A pCMV-CKAP4Δ236-439-3×HA Miaoling bio N/A pCMV-CKAP4Δ507-575-3×HA Miaoling bio N/A Bacterial and virus strains AAV9-F4/80-shCkap4-ZsGreen HanBio Technology N/A AAV9-F4/80-ZsGreen HanBio Technology N/A CKAP4-OE lentivirus GeneChem N/A Lentiviral negative control GeneChem N/A Software and algorithms FlowJo_V10 BD Biosciences https://www.flowjo.com GraphPad Prism 9.5.0 GraphPad Software https://www.graphpad.com ImageJ ImageJ https://imagej.nih.gov/ij AlphaFold3 DeepMind & Isomorphic Labs https://alphafoldserver.com PyMOL Schrödinger, LLC https://pymol.org RStudio Posit Software, PBC https://posit.co/rstudio CellProfiler Broad Institute of MIT and Harvard https://cellprofiler.org/ Cellpose-SAM Cellpose developers (Mouseland) https://www.cellpose.org Other Transparent wound dressing 3M Cat# 3589 Instant krazy glue Columbus Cat# 744-3514 AFM probe Bruker Cat# MLCT-SPH-5UM-DC 20 Cell Reports 45, 117395, June 23, 2026 Primary cell cultures and cell line Primary murine bone marrow-derived macrophages (BMDMs) were isolated from male WT and Ckap4− /− mice. ..

    Over Expression:

    Article Title: YARS2 mediates PARPi resistance in pancreatic cancer by regulating the MYC-E2F axis.
    Article Snippet: Background: Pancreatic ductal adenocarcinoma (PDAC) resistance to PARP inhibitors (PARPi) remains poorly understood, the mechanism of resistance remain poorly understood.. Material and Methods: We integrated multi-omics data from The Cancer Genome Atlas-Pancreatic Adenocarcinoma (TCGA-PAAD) datasets, Cancer Cell Line Encyclopedia (CCLE), and the Genomics of Drug Sensitivity in Cancer (GDSC) performed weighted gene co-expression network analysis (WGCNA) to identify hub genes linked to PARPi resistance.. GSE86394 from the Gene Expression Omnibus (GEO) database was utilized to differential expressed genes (DEGs) and performed functional enrichment analyses.

    Article Title: FTO Deficiency Inhibits Vascular Endothelial Cell Apoptosis and Improved Mitophagy in Obesity-Related Hypertension by Enhancing NDRG1 m6A Methylation.
    Article Snippet: Vascular endothelial dysfunction contributes to obesity-related hypertension, yet its mechanisms remain unclear.. This study examined the role of N6-methyladenosine (m6A) in this process using in vivo and in vitro models.. C57BL/6J mice fed a highfat diet (HFD) developed obesity-related hypertension, while oxidized low-density lipoprotein (ox-LDL)-treated human aortic endothelial cells (HAECs) simulated endothelial injury.

    Construct:

    Article Title: YARS2 mediates PARPi resistance in pancreatic cancer by regulating the MYC-E2F axis.
    Article Snippet: Background: Pancreatic ductal adenocarcinoma (PDAC) resistance to PARP inhibitors (PARPi) remains poorly understood, the mechanism of resistance remain poorly understood.. Material and Methods: We integrated multi-omics data from The Cancer Genome Atlas-Pancreatic Adenocarcinoma (TCGA-PAAD) datasets, Cancer Cell Line Encyclopedia (CCLE), and the Genomics of Drug Sensitivity in Cancer (GDSC) performed weighted gene co-expression network analysis (WGCNA) to identify hub genes linked to PARPi resistance.. GSE86394 from the Gene Expression Omnibus (GEO) database was utilized to differential expressed genes (DEGs) and performed functional enrichment analyses.

    Synthesized:

    Article Title: YARS2 mediates PARPi resistance in pancreatic cancer by regulating the MYC-E2F axis.
    Article Snippet: Background: Pancreatic ductal adenocarcinoma (PDAC) resistance to PARP inhibitors (PARPi) remains poorly understood, the mechanism of resistance remain poorly understood.. Material and Methods: We integrated multi-omics data from The Cancer Genome Atlas-Pancreatic Adenocarcinoma (TCGA-PAAD) datasets, Cancer Cell Line Encyclopedia (CCLE), and the Genomics of Drug Sensitivity in Cancer (GDSC) performed weighted gene co-expression network analysis (WGCNA) to identify hub genes linked to PARPi resistance.. GSE86394 from the Gene Expression Omnibus (GEO) database was utilized to differential expressed genes (DEGs) and performed functional enrichment analyses.

    shRNA:

    Article Title: Intratumoral microbiota promote pancreatic cancer progression via NaAc-mediated activation of the GPR43/AMPK/HADH pathway.
    Article Snippet: .. Short hairpin RNA (shRNA) targeting 3-hydroxyacyl-CoA dehydrogenase (HADH) and negative control shRNA (sh-NC) were generated by GeneChem (Shanghai). .. Small interfering RNA (siRNA) targeting G protein-coupled receptor 43 (GPR43) was constructed by GenePharma (Shanghai).

    Article Title: FTO Deficiency Inhibits Vascular Endothelial Cell Apoptosis and Improved Mitophagy in Obesity-Related Hypertension by Enhancing NDRG1 m6A Methylation.
    Article Snippet: Vascular endothelial dysfunction contributes to obesity-related hypertension, yet its mechanisms remain unclear.. This study examined the role of N6-methyladenosine (m6A) in this process using in vivo and in vitro models.. C57BL/6J mice fed a highfat diet (HFD) developed obesity-related hypertension, while oxidized low-density lipoprotein (ox-LDL)-treated human aortic endothelial cells (HAECs) simulated endothelial injury.

    Article Title: Identification and validation of programmed cell death-related genes in osteosarcoma: inhibiting CIB1 as a promising therapeutic strategy.
    Article Snippet: .. Cells were transduced with lentiviral particles carrying either negative control shRNA (NC-shRNA) or CIB1-targeting shRNA (GeneChem Corporation, Shanghai, China). ..

    Generated:

    Article Title: Intratumoral microbiota promote pancreatic cancer progression via NaAc-mediated activation of the GPR43/AMPK/HADH pathway.
    Article Snippet: .. Short hairpin RNA (shRNA) targeting 3-hydroxyacyl-CoA dehydrogenase (HADH) and negative control shRNA (sh-NC) were generated by GeneChem (Shanghai). .. Small interfering RNA (siRNA) targeting G protein-coupled receptor 43 (GPR43) was constructed by GenePharma (Shanghai).

    other:

    Article Title: Endothelial Nitric Oxide Synthase Restores Diabetic Dentin Regeneration via AKT/Endothelial Nitric Oxide Synthase Axis
    Article Snippet: For flow cytometric analysis of surface immunophenotypic markers, DPSCs were washed three times with PBS and then incubated with fluorescein isothiocyanate (FITC)- or R-phycoerythrin (PE)-conjugated mouse anti-human monoclonal antibodies against the following surface antigens: CD73, CD34, CD45, CD90, CD105, and HLA-DR (BioLegend, USA).

    Transduction:

    Article Title: Identification and validation of programmed cell death-related genes in osteosarcoma: inhibiting CIB1 as a promising therapeutic strategy.
    Article Snippet: .. Cells were transduced with lentiviral particles carrying either negative control shRNA (NC-shRNA) or CIB1-targeting shRNA (GeneChem Corporation, Shanghai, China). ..



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    Image Search Results


    Journal: Neuron

    Article Title: Major Depressive Disorder associated dysregulation of ZBTB7A in orbitofrontal cortex promotes astrocyte-mediated stress susceptibility

    doi: 10.1016/j.neuron.2025.05.023

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Negative control sequence without 5’ overhangs: GAAATGTACTGCGCGTGGAGACGTTTTGGCCACTGACTGACGTCTCCACGCAGTACATTT , ThermoFisher , Cat # K493600.

    Techniques: Virus, Generated, Cloning, Expressing, Recombinant, Blocking Assay, Plasmid Preparation, Negative Control, Sequencing, Software