mutational profiling map fragmentation buffer (Zymo Research)
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Mutational Profiling Map Fragmentation Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 95/100, based on 235 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mutation+profiling/Digestion+Buffer/pmc12000875-61-8-54
Average 95 stars, based on 235 article reviews
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Centrifugation:Article Title: Combined-stress priming alters physiology, transcriptional dynamics, and acute thermal resistance in Acropora cervicornis corals Article Snippet: Samples were ground into a homogeneous powder with a mortar, pestle, and liquid nitrogen, then 300 mg of powder was further homogenized with bead beating (pre-filled Zymo DNA/RNA Shield and 2.0 mm BashingBeads tubes) for two 1 min rounds (Biospec Products), alternated with 1 min on ice. .. Homogenized samples were further digested with Extraction:Article Title: Combined-stress priming alters physiology, transcriptional dynamics, and acute thermal resistance in Acropora cervicornis corals Article Snippet: Samples were ground into a homogeneous powder with a mortar, pestle, and liquid nitrogen, then 300 mg of powder was further homogenized with bead beating (pre-filled Zymo DNA/RNA Shield and 2.0 mm BashingBeads tubes) for two 1 min rounds (Biospec Products), alternated with 1 min on ice. .. Homogenized samples were further digested with Immunoprecipitation:Article Title: A genome language model for mapping DNA replication origins Article Snippet: Adapter ligated DNA was then denatured and incubated with anti-BrdU antibody (12.5 μg/mL; BD cat. no. 555627), followed by immuno-precipitation with Rabbit anti-mouse IgG (20μg; Sigma-Aldrich, cat. no. M7023). .. Immunoprecipitated DNA was resuspended in Purification:Article Title: A genome language model for mapping DNA replication origins Article Snippet: Adapter ligated DNA was then denatured and incubated with anti-BrdU antibody (12.5 μg/mL; BD cat. no. 555627), followed by immuno-precipitation with Rabbit anti-mouse IgG (20μg; Sigma-Aldrich, cat. no. M7023). .. Immunoprecipitated DNA was resuspended in Article Title: Endometriomas are heterogeneous for functional FSH receptor expression and steroidogenic function. Article Snippet: We investigated whether ovarian endometriomas uniformly express functional FSH receptors (FSHR) and respond to FSH by activating transcriptional programs that drive aromatase and steroid hormone production.. Endometrioma cyst capsules were obtained from 18 patients undergoing surgery between January 2020 and December 2024.. FSHR and steroidogenic pathway components were evaluated using qRT-PCR, quantitative immunoblotting, and immunohistochemistry. Concentration Assay:Article Title: Spatially resolved gene expression analysis illuminates location-specific functions in the reef-building coral Pocillopora acuta Article Snippet: .. Caps contained 40 μL of a freshly prepared Recombinant:Article Title: Cell-type-specific transposon demethylation and TAD remodeling in aging mouse brain. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488–conjugated anti-NeuN antibody Millipore Cat# MAB377X; Clone A60; RRID: AB_2149209 Chemicals, peptides, and recombinant proteins Hoechst 33342 Thermo Fisher Scientific Cat# 62249 Proteinase K Zymo Cat# D3001-2-20 CT Conversion Reagent Zymo Cat# D5003-1 Article Title: Expression and Purification of the Ectodomain of Erythropoietin Receptor Fused to mCitrine or mTFP1 Fluorescent Protein in Escherichia coli. Article Snippet: The erythropoietin receptor (EPOR) is a single-pass transmembrane protein that homo-dimerizes upon binding with its renal ligand erythropoietin (EPO) to trigger downstream signaling.. Its extracellular ectodomain mediates ligand binding.. Therefore, a fusion protein of the EPOR ectodomain can be useful for various in vitro assays, such as a binding assay with an EPO-like peptide, if overexpressed in Escherichia coli (E.coli). Single Cell:Article Title: Cell-type-specific transposon demethylation and TAD remodeling in aging mouse brain. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488–conjugated anti-NeuN antibody Millipore Cat# MAB377X; Clone A60; RRID: AB_2149209 Chemicals, peptides, and recombinant proteins Hoechst 33342 Thermo Fisher Scientific Cat# 62249 Proteinase K Zymo Cat# D3001-2-20 CT Conversion Reagent Zymo Cat# D5003-1 Sample Prep:Article Title: Cell-type-specific transposon demethylation and TAD remodeling in aging mouse brain. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488–conjugated anti-NeuN antibody Millipore Cat# MAB377X; Clone A60; RRID: AB_2149209 Chemicals, peptides, and recombinant proteins Hoechst 33342 Thermo Fisher Scientific Cat# 62249 Proteinase K Zymo Cat# D3001-2-20 CT Conversion Reagent Zymo Cat# D5003-1 Imaging:Article Title: Cell-type-specific transposon demethylation and TAD remodeling in aging mouse brain. Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Alexa Fluor 488–conjugated anti-NeuN antibody Millipore Cat# MAB377X; Clone A60; RRID: AB_2149209 Chemicals, peptides, and recombinant proteins Hoechst 33342 Thermo Fisher Scientific Cat# 62249 Proteinase K Zymo Cat# D3001-2-20 CT Conversion Reagent Zymo Cat# D5003-1 Transformation Assay:Article Title: Expression and Purification of the Ectodomain of Erythropoietin Receptor Fused to mCitrine or mTFP1 Fluorescent Protein in Escherichia coli. Article Snippet: The erythropoietin receptor (EPOR) is a single-pass transmembrane protein that homo-dimerizes upon binding with its renal ligand erythropoietin (EPO) to trigger downstream signaling.. Its extracellular ectodomain mediates ligand binding.. Therefore, a fusion protein of the EPOR ectodomain can be useful for various in vitro assays, such as a binding assay with an EPO-like peptide, if overexpressed in Escherichia coli (E.coli). DNA Amplification:Article Title: Expression and Purification of the Ectodomain of Erythropoietin Receptor Fused to mCitrine or mTFP1 Fluorescent Protein in Escherichia coli. Article Snippet: The erythropoietin receptor (EPOR) is a single-pass transmembrane protein that homo-dimerizes upon binding with its renal ligand erythropoietin (EPO) to trigger downstream signaling.. Its extracellular ectodomain mediates ligand binding.. Therefore, a fusion protein of the EPOR ectodomain can be useful for various in vitro assays, such as a binding assay with an EPO-like peptide, if overexpressed in Escherichia coli (E.coli). Over Expression:Article Title: Expression and Purification of the Ectodomain of Erythropoietin Receptor Fused to mCitrine or mTFP1 Fluorescent Protein in Escherichia coli. Article Snippet: The erythropoietin receptor (EPOR) is a single-pass transmembrane protein that homo-dimerizes upon binding with its renal ligand erythropoietin (EPO) to trigger downstream signaling.. Its extracellular ectodomain mediates ligand binding.. Therefore, a fusion protein of the EPOR ectodomain can be useful for various in vitro assays, such as a binding assay with an EPO-like peptide, if overexpressed in Escherichia coli (E.coli). other:Article Title: Defining bottlenecks and physiological impact of an orthogonal translation initiation system Article Snippet: Oxidated small RNA was recovered using the Micro Bio Spin P30 columns (BioRad, 7326223) to buffer exchange, and the Tissue:Article Title: Endometriomas are heterogeneous for functional FSH receptor expression and steroidogenic function. Article Snippet: We investigated whether ovarian endometriomas uniformly express functional FSH receptors (FSHR) and respond to FSH by activating transcriptional programs that drive aromatase and steroid hormone production.. Endometrioma cyst capsules were obtained from 18 patients undergoing surgery between January 2020 and December 2024.. FSHR and steroidogenic pathway components were evaluated using qRT-PCR, quantitative immunoblotting, and immunohistochemistry. |
