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rt-pcr detecting specific spike mutation profiles allplex sars-cov-2 variants i assay  (Seegene Technologies)

 
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    Seegene Technologies rt-pcr detecting specific spike mutation profiles allplex sars-cov-2 variants i assay
    Rt Pcr Detecting Specific Spike Mutation Profiles Allplex Sars Cov 2 Variants I Assay, supplied by Seegene Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mutation+profiling/allplex+sars+cov+2+assay/pm38570699-51-23-34
    Average 90 stars, based on 1 article reviews
    rt-pcr detecting specific spike mutation profiles allplex sars-cov-2 variants i assay - by Bioz Stars, 2026-10
    90/100 stars

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    Article Title: Association of IL10 gene SNVs rs1800896 (A>G), rs1800871 (C>T), rs1800872 (C>A) and haplotypes with COVID-19 severity and outcome in the Brazilian population.
    Article Snippet: Background: Elevated concentrations of IL-10 have been detected in coronavirus disease (COVID-19) patients and are a possible disease severity marker.. Single nucleotide variants (SNVs) and their haplotypes can be associated with differences in IL-10 levels and with viral disease susceptibility.. Aim: Evaluate the associations of SNVs and their haplotypes in Brazilian patients with COVID-19 severity and outcome.

    Article Title: Evaluation of the cobas SARS-CoV-2 & Influenza A/B v2 assay for detecting SARS-CoV-2 and influenza A and B viruses in nasopharyngeal swab specimens.
    Article Snippet: In the present study, we evaluated the cobas SARS-CoV-2 & Influenza A/B v2 assay (Roche Diagnostics, Basel, Switzerland) using the Roche cobas 5800 system, comparing it to the Allplex SARS-CoV-2/FluA/ FluB/RSV assay (Seegene, Seoul, Republic of Korea).

    Article Title: Profiles of viral pathogens from individuals with acute respiratory tract infections in northern Tanzania
    Article Snippet: Amplification and detection of SARS-CoV-2, Flu A, Flu B, and RSV Preparations for amplification and detection of the four viral targets namely SARS-CoV2, FluA, FluB, and RSV was done using AllplexTM SARS-CoV-2/FluA/FluB/RSV RT-PCR assay kit (Seegene Inc, Seoul, Republic of Korea).

    Article Title: Evaluation of two SARS-CoV-2 whole genome amplification and sequencing workflows for genomic surveillance and outbreak analysis in the clinical microbiology laboratory.
    Article Snippet: The presence of SARS-CoV-2 was confirmed by RT-PCR in a single step with the AllplexTM SARS-CoV-2/ FluA/FluB/RSV Assay (Seegene) on CFX96 instruments (BIO-RAD, California, USA).

    Article Title: Incidence of SARS-CoV-2 reinfection among blood donors from two Brazilian states in the post-vaccination period: a prospective cohort study
    Article Snippet: Subsequently, the Allplex TM SARS-CoV-2/FluA/FluB/RSV multiplex assay, a real-time reverse transcription polymerase chain reaction, was conducted to detect and differentiate the N, RdRP, and S genes of SARS-CoV-2 (Seegene Inc, Seoul, KR).

    Article Title: Effective surveillance of acute COVID-19 using a cost- and labor-efficient approach: a paradigm for the longitudinal monitoring of respiratory infections in larger cohorts.
    Article Snippet: Evaluation of the clinical and analytical performance of the seegene allplexTM SARS-CoV-2 variants I assay for the detection of variants of concern (VOC) and variants of interests (VOI).

    Article Title: Profiles of viral pathogens from individuals with acute respiratory tract infections in northern Tanzania
    Article Snippet: Preparations for amplification and detection of the four viral targets, namely, SARS-CoV-2, FluA, FluB, and RSV, was done using Allplex SARS-CoV-2/FluA/FluB/RSV RT-PCR assay kit (Seegene Inc, Seoul, Republic of Korea).

    Biomarker Discovery:

    Article Title: Respiratory dysbiosis as prognostic biomarker of disease severity for adults with community-acquired pneumonia requiring mechanical ventilation.
    Article Snippet: The microbiological diagnosis of pneumonia was performed via routine, well-established techniques: bloodcultures (BD BACTECTM blood culture systems), urine antigen test for Streptococcus pneumoniae and Legionella pneumophila detection (Sofia FIA® Quidel Corporation, San Diego, CA, USA), qRT-PCR targeting viral respiratory pathogens (AllplexTM Respiratory Panels 1, 2 and 3, Seegene, South Korea), PCR of a pharyngeal swab for other less frequent bacterial pathogens (Mycoplasma pneumoniae and Chlamydophila pneumoniae, CerTest Biotec SL, Spain). .. The diagnosis of SARS-CoV-2 pneumonia had been commonly done some days before admission using commercial RT-PCR on nasopharyngeal swabs (AllplexTM SARS-CoV-2 assay, Seegene, South Korea). .. The analysis of the lung microbiome was determined via direct or nested PCRs if necessary using the Ion 16S Metagenomics Kit (Thermo Fisher Scientific) from nucleic acids extracted from mini-BALs as previously detailed [7].

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Respiratory dysbiosis as prognostic biomarker of disease severity for adults with community-acquired pneumonia requiring mechanical ventilation.
    Article Snippet: The microbiological diagnosis of pneumonia was performed via routine, well-established techniques: bloodcultures (BD BACTECTM blood culture systems), urine antigen test for Streptococcus pneumoniae and Legionella pneumophila detection (Sofia FIA® Quidel Corporation, San Diego, CA, USA), qRT-PCR targeting viral respiratory pathogens (AllplexTM Respiratory Panels 1, 2 and 3, Seegene, South Korea), PCR of a pharyngeal swab for other less frequent bacterial pathogens (Mycoplasma pneumoniae and Chlamydophila pneumoniae, CerTest Biotec SL, Spain). .. The diagnosis of SARS-CoV-2 pneumonia had been commonly done some days before admission using commercial RT-PCR on nasopharyngeal swabs (AllplexTM SARS-CoV-2 assay, Seegene, South Korea). .. The analysis of the lung microbiome was determined via direct or nested PCRs if necessary using the Ion 16S Metagenomics Kit (Thermo Fisher Scientific) from nucleic acids extracted from mini-BALs as previously detailed [7].



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    Image Search Results


    (A) Mutational frequencies of the top mutated genes identified in the 72 non‐hypermutated gastric cancer (GC) tumors from PRH ( n = 72 for all genes except ARID1A ( n = 62), KMT2D ( n = 70), MYH11 ( n = 63), IRS2 ( n = 65), and CLTCL1 ( n = 61)). (B) Mutational frequencies by anatomical location of non‐hypermutated GC tumors from PRH. For Fundus/Cardia, there were 13 cases, and n = 13 for all genes, except for MYH11 , PHOX2B , and PER1 , which had n = 12. For the Body, there were 45 cases, and all genes had n = 45, except for ARID1A ( n = 38), KMT2D ( n = 43), and CACNA1D ( n = 39). For the Antrum, there were 14 cases, and all genes had n = 14, except for NOTCH1 ( n = 12), CDKN2A ( n = 13), and NFE2L2 ( n = 13).

    Journal: Cancer Medicine

    Article Title: Actionable Genes and Carcinogenic Pathways for Gastric Cancer in Latinos

    doi: 10.1002/cam4.71216

    Figure Lengend Snippet: (A) Mutational frequencies of the top mutated genes identified in the 72 non‐hypermutated gastric cancer (GC) tumors from PRH ( n = 72 for all genes except ARID1A ( n = 62), KMT2D ( n = 70), MYH11 ( n = 63), IRS2 ( n = 65), and CLTCL1 ( n = 61)). (B) Mutational frequencies by anatomical location of non‐hypermutated GC tumors from PRH. For Fundus/Cardia, there were 13 cases, and n = 13 for all genes, except for MYH11 , PHOX2B , and PER1 , which had n = 12. For the Body, there were 45 cases, and all genes had n = 45, except for ARID1A ( n = 38), KMT2D ( n = 43), and CACNA1D ( n = 39). For the Antrum, there were 14 cases, and all genes had n = 14, except for NOTCH1 ( n = 12), CDKN2A ( n = 13), and NFE2L2 ( n = 13).

    Article Snippet: We examined tumor mutational profiles of GC from 106 PRH between 2015 and 2022 (provided by CARIS Life Sciences and the Precision Oncology Alliance).

    Techniques:

    Frequency of genetic alterations in key signaling pathways in non‐hypermutated gastric cancer cases leading to deregulation of the WNT/β‐catenin, MAPK, PI3K, TGF‐β, and p53 signaling pathways in the non‐hypermutated cases. The frequencies of alterations are expressed as a percentage of all cases. The red color denotes the overall mutational frequency, and blue denotes the amplification frequency for each gene. Figure adapted from: Nature, volume 487, pages 330–337 (2012).

    Journal: Cancer Medicine

    Article Title: Actionable Genes and Carcinogenic Pathways for Gastric Cancer in Latinos

    doi: 10.1002/cam4.71216

    Figure Lengend Snippet: Frequency of genetic alterations in key signaling pathways in non‐hypermutated gastric cancer cases leading to deregulation of the WNT/β‐catenin, MAPK, PI3K, TGF‐β, and p53 signaling pathways in the non‐hypermutated cases. The frequencies of alterations are expressed as a percentage of all cases. The red color denotes the overall mutational frequency, and blue denotes the amplification frequency for each gene. Figure adapted from: Nature, volume 487, pages 330–337 (2012).

    Article Snippet: We examined tumor mutational profiles of GC from 106 PRH between 2015 and 2022 (provided by CARIS Life Sciences and the Precision Oncology Alliance).

    Techniques: Protein-Protein interactions, Amplification