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rneasy mini kit  (Qiagen)


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    Qiagen rneasy mini kit
    Rneasy Mini Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 40568 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/multi-analyte+elisarray+kit/RNeasy+Mini+Kit/pmc06356400-50-5-19
    Average 99 stars, based on 40568 article reviews
    rneasy mini kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Extracellular biogenic nanoscale mitochondria reprogram the wound microenvironment via ROS scavenging independent of cellular uptake
    Article Snippet: .. Total RNA was extracted from cultured cells and peri-wound tissue samples using the RNeasy Mini Kit (Qiagen, Cat# 74014), following the manufacturer's protocol. .. Reverse transcription was performed using the PrimeScriptTM RT Master Mix (Takara, Cat# RR036A).

    Synthesized:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    Reverse Transcription:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Real-time Polymerase Chain Reaction:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    SYBR Green Assay:

    Article Title: METTL1 Aggravates Periodontitis Progression by Inhibiting Osteoblast Differentiation via m7G modification of SEMA4D
    Article Snippet: These plasmids were transfected into HPDLSCs using Lipofectamine 3000 (Invitrogen, Carlsbad, CA, USA). .. Total RNA was extracted from HPDLSCs using the RNeasy Mini Kit (Qiagen, Hilden, Germany). cDNA was synthesized from 1 μg of total RNA using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA, USA). qPCR was performed using the PowerUp SYBR Green Master Mix (Applied Biosystems, Foster City, CA, USA) on the QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA). ..

    Isolation:

    Article Title: Effects of dietary Chlorella vulgaris and cinnamon oil on growth performance, gut morphology, digestive enzymes, and intestinal gene expression in broilers
    Article Snippet: .. Total RNA was isolated from jejunal tissue samples using TRIzol® reagent (Invitrogen Life Technologies, Palo Alto, CA, USA) and subsequently purified with the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The integrity of RNA was verified by agarose gel electrophoresis, and concentrations were determined with a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    Article Title: Obesity as a Determinant of Periodontal Therapy Outcomes: Insights on Oxidative and Endoplasmic Reticulum Stress Pathways
    Article Snippet: Membranes were incubated with mouse lgG secondary antibodies (Thermo Fisher Scientific Cat# 31430, RRID:AB_228307) and chemiluminescence was detected using ECL Plus reagent (Cell Signalling, Danvers, MA, USA), visualized with a Fusion FX5 system, and quantified by densitometry using Bio1D software ( https://www.vilber.com ,(RRID:SCR_023580). .. Total RNA was isolated from PBMCs using the RNeasy Mini Kit (Qiagen, Venlo, Netherlands), following the manufacturer’s protocol. .. Subsequently, 1 μg of RNA was reverse transcribed into cDNA using the RevertAid First Strand cDNA Synthesis Kit (Thermo Scientific, Waltham, MA, USA). qPCR was performed employing the FastStart Universal SYBR Green Master (Roche Applied Science, Penzberg, Germany) in a 7500 Fast Real-Time qPCR System (Life Technologies, Carlsbad, CA, USA).

    Article Title: Identification of small molecules that enhance aminoglycoside-mediated suppression of CFTR and NF1 nonsense mutations
    Article Snippet: .. Total RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Qiagen, 74106) with optional QIAshredder (Qiagen, 79656). .. 1 μg of RNA was reverse transcribed using BioRad iScript reverse transcriptase (Bio-Rad, 1708841) in a Bio-Rad C1000 Touch Thermal Cycler.

    Purification:

    Article Title: Effects of dietary Chlorella vulgaris and cinnamon oil on growth performance, gut morphology, digestive enzymes, and intestinal gene expression in broilers
    Article Snippet: .. Total RNA was isolated from jejunal tissue samples using TRIzol® reagent (Invitrogen Life Technologies, Palo Alto, CA, USA) and subsequently purified with the RNeasy Mini Kit (Qiagen, Valencia, CA, USA). .. The integrity of RNA was verified by agarose gel electrophoresis, and concentrations were determined with a NanoDrop 1000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA).

    RNA Extraction:

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. RNA extraction of cells was carried out using the RNeasy mini kit (Qiagen, Ireland) according to the manufacturer's protocol, and the extracted RNA was eluted into molecular grade H 2 O. RNA quantity and quality were then assessed using a NanoDrop (NeoDot – UV/VIS Nano Spectrophotometer; Neo Biotech). .. Between 200 ng and 400ng of RNA (ensuring uniform amounts between samples and within experiments) were reverse transcribed using a QuantiTect reverse transcription kit as per the manufacturer's instructions (Qiagen, Ireland).

    Spectrophotometry:

    Article Title: Development of a PTEN -siRNA activated scaffold to promote axonal regrowth following spinal cord injury
    Article Snippet: .. RNA extraction of cells was carried out using the RNeasy mini kit (Qiagen, Ireland) according to the manufacturer's protocol, and the extracted RNA was eluted into molecular grade H 2 O. RNA quantity and quality were then assessed using a NanoDrop (NeoDot – UV/VIS Nano Spectrophotometer; Neo Biotech). .. Between 200 ng and 400ng of RNA (ensuring uniform amounts between samples and within experiments) were reverse transcribed using a QuantiTect reverse transcription kit as per the manufacturer's instructions (Qiagen, Ireland).

    Expressing:

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).

    cDNA Synthesis:

    Article Title: Spinal cord extracellular matrix hydrogel enhances organoid maturation and functional regeneration after spinal cord injury
    Article Snippet: The area of SCO cultured in each hydrogel condition was measured using bright-field images obtained through an optical microscopy (EVOSTM FL Auto Imaging System, Thermo Fisher Scientific) and analyzed with ImageJ software. .. The mRNA expression of SCO was quantified with qPCR analysis. mRNA samples were prepared using RNeasy Mini Kit (74106, QIAGEN GmbH, Hamburg, Germany), and cDNA samples were synthesized from the extracted mRNA using a cDNA Synthesis Kit (TakaRa). .. Subsequently, qPCR analysis was conducted using cDNA samples along with TaqMan Fast Universal PCR Master Mix (Applied Biosystems, Foster City, CA, USA) on a StepOnePlus Real-Time PCR System (Applied Biosystems).



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    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory <t>Cytokine</t> <t>ELISArray</t> Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.
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    Image Search Results


    The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

    Journal: Cells

    Article Title: Signalling Pathways of Inflammation and Cancer in Human Mononuclear Cells: Effect of Nanoparticle Air Pollutants

    doi: 10.3390/cells13161367

    Figure Lengend Snippet: The effect of carbon black (CB), urban dust (UD), and nanoparticulate carbon black (NPCB) on cell membrane damage (( A ); lactate dehydrogenase (LDH) release), oxidative stress ( B ), DNA damage ( C ), and phagocytosis ( D ) in human monocytes (M), and monocyte-derived macrophages (MDM). The inflammatory response (TNFα; ( E )) to lipopolysaccharide (100 ng/mL for 24 h) is also included ( F ). All PMs were used at 100 µg/mL and the incubation time was 24 h. Cell transition was induced by phorbol-12-myristate 13-acetate (PMA; 100 nM) applied for 72 h. LDH was measured with CyQUANT LDH Cytotoxicity Assay kit, DNA ploidy was quantified using propidium iodide staining, intracellular oxidative stress was measured using 5-(and-6)-Carboxy-2′,7′-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA) while phagocytosis was assessed with fluorescent beads (Cayman Phagocytosis kit). Fluorescence was quantified with flow cytometry. TNFα was measured in the culture medium using a Multi-Analyte Inflammatory Cytokine ELISArray Kit (Quiagen, Wroclaw, Poland). Data are means ± SD of 6–10 assays. * p < 0.05; ** p < 0.01 for comparisons with the corresponding control cells; ^^ p < 0.01 for comparisons with corresponding naive cells; # p < 0.05 for comparisons with LPS-treated cells.

    Article Snippet: TNFα was quantified in the culture medium of M and MDM with the Multi-Analyte Inflammatory Cytokine ELISArray Kit (Qiagen, Manchester, UK).

    Techniques: Membrane, Derivative Assay, Incubation, CyQUANT Assay, LDH Cytotoxicity Assay, Staining, Fluorescence, Flow Cytometry, Control