cdna (New England Biolabs)

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New England Biolabs cdna

Transcriptome analysis of 59 selected genes associated with oral chemosensation, displayed as a heatmap showing mean fold changes in gene expression of the SSB-fed diet groups in relation to the respective water-fed group (=1) in the form of a color code. The gene expression was analyzed using one customized <t>cDNA</t> microarray per group from <t>pooled</t> <t>RNA</t> samples of the CV from mice that received either a standard diet (chow, n = 10–11) or Western-type diet (WD, n = 7–8) with water (Water) or a sugar-sweetened beverage (SSB) as a drink for 24 weeks.

Cdna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 97/100, based on 1267 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mrna+analysis+dna+microarray/LunaScript+RT+SuperMix/pmc08837962-92-10-17
Average 97 stars, based on 1267 article reviews

cdna - by Bioz Stars, 2026-10

97/100 stars

Images

1) Product Images from "Long-Term Consumption of a Sugar-Sweetened Soft Drink in Combination with a Western-Type Diet Is Associated with Morphological and Molecular Changes of Taste Markers Independent of Body Weight Development in Mice"

Article Title: Long-Term Consumption of a Sugar-Sweetened Soft Drink in Combination with a Western-Type Diet Is Associated with Morphological and Molecular Changes of Taste Markers Independent of Body Weight Development in Mice

Journal: Nutrients

doi: 10.3390/nu14030594

Figure Legend Snippet: Transcriptome analysis of 59 selected genes associated with oral chemosensation, displayed as a heatmap showing mean fold changes in gene expression of the SSB-fed diet groups in relation to the respective water-fed group (=1) in the form of a color code. The gene expression was analyzed using one customized cDNA microarray per group from pooled RNA samples of the CV from mice that received either a standard diet (chow, n = 10–11) or Western-type diet (WD, n = 7–8) with water (Water) or a sugar-sweetened beverage (SSB) as a drink for 24 weeks.

Techniques Used: Expressing, Microarray, Western Blot

other:

Article Title: Food-Relevant Polycyclic Aromatic Hydrocarbons Disrupt Intestinal Barrier and Xenobiotic Metabolism in Mouse, Pig, and Human Epithelial Cells.
Article Snippet: Following this, the cDNA was prepared using the LunaScript RT SuperMix 275 Kit (New England Biolabs, Ipswich, MA) with 1.0-2.0 μg of total RNA.

Synthesized:

Article Title: Targeting catecholate siderophore systems enables precision depletion of gut enterobacteria during colitis
Article Snippet: RNA was extracted by transferring tissues to RINO Bead Lysis kit tubes (Next Advance) and homogenizing in Tri-Reagent (Molecular Research Center) on a Bullet Blender Tissue Homogenizer (Next Advance). .. Lysates were then transferred to EconoSpin RNA Mini Spin Columns (Epoch Life Science Inc.), DNA was degraded using the PureLink on-column DNase Set (Life Technologies), then cDNA was synthesized from 1 μg input RNA using LunaScript RT SuperMix (NEB). .. Gene expression was quantified by qPCR using the PowerUp SYBR Green Master Mix (Life Technologies) and reactions were amplified on a QuantStudio 5 Real-Time PCR System (Thermo Fisher).

Article Title: Tissue-Specific Expression and Laying-Performance Responsiveness of FXR1 PRL, GnRH2, and TFPI2 in Helmeted Guinea Fowl
Article Snippet: .. First-strand cDNA was synthesized from total RNA extracted from brain, liver and ovary tissues using Luna Script RT Super Mix (New England Biolabs, USA). .. The reverse-transcription reaction was performed in a final volume of 20 μL containing 4 μL of 5X Luna Script RT Super Mix and the appropriate amount of RNA template, with nuclease-free water added to obtain the final reaction volume.

Article Title: Structural mechanism of Gasdermin E-mediated mtDNA release from apoptotic mitochondria
Article Snippet: .. 200 ng RNA were synthesized to cDNA with the LunaScript RT SuperMix (New England Biolabs, cat no: M3010X). .. RT-qPCR was performed using the Luna Universal qPCR Master Mix (New England Biolabs, cat no: M3003E) with 20 ng cDNA per reaction.

Reverse Transcription:

Article Title: Human trilayer engineered blood vessel reveals influence of fibroblasts on disease progression in model of Hutchinson-Gilford progeria.
Article Snippet: RNA purity and concentrations were measured using a Nanodrop (Thermo Fisher Scientific). .. cDNA was reverse transcribed from 250 μg (cells) or 125 μg (TEBVs) of RNA using LunaScript RT SuperMix (New England Biolabs) in an MWG- Biotech Primus 25 Thermal Cycler (Cole- Parmer). .. The synthesized cDNA was used in qPCR reactions with iQ SYBR Green Supermix (Bio- Rad), and the primers were listed in table S12 (Integrated DNA Technologies).

Article Title: Loss of PmpC in Chlamydia trachomatis leads to altered bacterial development and impaired recovery from IFNγ- and penicillin-induced stress.
Article Snippet: .. Subsequently, 1 μg of the DNA-free RNA was reverse-transcribed into cDNA employing LunaScript RT SuperMix (New England Biolabs). ..

DNA Synthesis:

Article Title: Implementation of rapid pan-microbial metagenomics in paediatric intensive care: a single-centre, prospective, observational study.
Article Snippet: A modification to the previously published workflow was made to the extraction method, whereby 200 μL of sample was extracted using the EZ1 Advanced XL with the Virus Mini Kit as per manufacturer’s instructions (Qiagen, Hilden, Germany), excluding carrier RNA, and eluted into 60 μL. .. cDNA was synthesised using LunaScript RT SuperMix (New England Biolabs, Ipswich, MA, USA), followed by double-stranded DNA synthesis using Sequenase Version 2.0 DNA Polymerase (Applied Biosystems, Waltham, MA, USA). .. DNA library preparation was performed with SQK-RPB004 kit, followed by sequencing for 24 h on the GridION Mk1 sequencer (Oxford Nanopore Technologies, Oxford, UK).

Control:

Article Title: OPHN1-related neurodevelopmental disorders: clinical and genetic findings with novel variants.
Article Snippet: Extended author information available on the last page of the article Abstract Pathogenic variants in the OPHN1 gene are associated with X-linked intellectual developmental disorder, Billuart type (OMIM #300486), characterized by intellectual disability, distinctive facial features, cerebellar abnormalities, ventriculomegaly, epilepsy, and variable neuropsychiatric manifestations.. In this study, four male individuals from four unrelated families presenting with intellectual disability and developmental delay were evaluated.. Comprehensive clinical, neuroimaging, and molecular genetic data were collected.

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