maui hybridization system (BioMicro Systems Inc)
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Maui Hybridization System, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/maui+hybridization+system/pmc04571549-38-36-39
Average 90 stars, based on 1 article reviews
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Hybridization:Article Title: Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1 Article Snippet: Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides. .. Thirty five thousand oligonucleotide arrays were hybridized at 42°C in Article Title: Soil pH Filters the Association Patterns of Aluminum-Tolerant Microorganisms in Rice Paddies Article Snippet: .. The fluorescently labeled DNA was hybridized with GeoChip 5.0 on a Article Title: Stimulation of soil microbial functioning by elevated CO2 may surpass effects mediated by irrigation in a semiarid grassland Article Snippet: The functioning of microbial communities in response to elevated atmospheric carbon dioxide (eCO2) is one of the most important, but poorly understood, contributors to climate change feedbacks.. The effect of soil moisture caused by eCO2 on soil microbial communities and functioning may be of high importance in water limited ecosystems.. To address this knowledge gap, we measured the functional composition, structure, and functional processes of soil microbial communities under eCO2 and an irrigation treatment simulating water availability with eCO2 at two soil depths (0–5 cm and 5–15 cm) in a mixed-grass semiarid prairie. Article Title: Functional Overlap between eIF4G Isoforms in Saccharomyces cerevisiae Article Snippet: Remaining RNA was acid hydrolysed and cDNA purified using Qiagen MinElute columns. .. The resulting cDNA was labeled with Cy3 or Cy5 (GE healthcare PA23001 or PA25001) via the aa-dU nucleoside, and hybridized, using the Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs. Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90 ̊C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65 ̊C using the Article Title: Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection Article Snippet: .. Labeled samples (1.5 μg) were hybridized at 42°C to B. melitensis (Nimblegen A4357-001-01) or custom designed arrays (Nimblegen), using a 1× mixing chamber (Nimblegen) and Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90°C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65°C using the Labeling:Article Title: Soil pH Filters the Association Patterns of Aluminum-Tolerant Microorganisms in Rice Paddies Article Snippet: .. The fluorescently labeled DNA was hybridized with GeoChip 5.0 on a Article Title: Stimulation of soil microbial functioning by elevated CO2 may surpass effects mediated by irrigation in a semiarid grassland Article Snippet: The functioning of microbial communities in response to elevated atmospheric carbon dioxide (eCO2) is one of the most important, but poorly understood, contributors to climate change feedbacks.. The effect of soil moisture caused by eCO2 on soil microbial communities and functioning may be of high importance in water limited ecosystems.. To address this knowledge gap, we measured the functional composition, structure, and functional processes of soil microbial communities under eCO2 and an irrigation treatment simulating water availability with eCO2 at two soil depths (0–5 cm and 5–15 cm) in a mixed-grass semiarid prairie. Article Title: Functional Overlap between eIF4G Isoforms in Saccharomyces cerevisiae Article Snippet: Remaining RNA was acid hydrolysed and cDNA purified using Qiagen MinElute columns. .. The resulting cDNA was labeled with Cy3 or Cy5 (GE healthcare PA23001 or PA25001) via the aa-dU nucleoside, and hybridized, using the Article Title: Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection Article Snippet: .. Labeled samples (1.5 μg) were hybridized at 42°C to B. melitensis (Nimblegen A4357-001-01) or custom designed arrays (Nimblegen), using a 1× mixing chamber (Nimblegen) and Microarray:Article Title: Soil pH Filters the Association Patterns of Aluminum-Tolerant Microorganisms in Rice Paddies Article Snippet: .. The fluorescently labeled DNA was hybridized with GeoChip 5.0 on a other:Article Title: Phylogenetic Patterns in the Microbial Response to Resource Availability: Amino Acid Incorporation in San Francisco Bay Article Snippet: Reagents for synthesis were delivered through an Expedite system (PerSeptive Biosystems, Framingham, MA, USA). Incubation:Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs. Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90 ̊C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65 ̊C using the Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90°C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65°C using the Mixing Chamber:Article Title: Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection Article Snippet: .. Labeled samples (1.5 μg) were hybridized at 42°C to B. melitensis (Nimblegen A4357-001-01) or custom designed arrays (Nimblegen), using a 1× mixing chamber (Nimblegen) and |
