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BioMicro Systems Inc maui hybridization system
Maui Hybridization System, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/maui+hybridization+system/pmc04571549-38-36-39
Average 90 stars, based on 1 article reviews
maui hybridization system - by Bioz Stars, 2026-11
90/100 stars

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Hybridization:

Article Title: Establishment and Characterization of a Novel Head and Neck Squamous Cell Carcinoma Cell Line USC-HN1
Article Snippet: Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides. .. Thirty five thousand oligonucleotide arrays were hybridized at 42°C in MAUI hybridization system (BioMicro Systems) for overnight, washed with 1 × SSC with 0.05% SDS and 0.1 × SSC buffers, and then were quickly spin-dried. .. Labeled and combined cDNA probes were denatured, mixed in SlideHyb #1 hybridization buffer (Ambion, Austin, TX), and placed onto microarray slides.

Article Title: Soil pH Filters the Association Patterns of Aluminum-Tolerant Microorganisms in Rice Paddies
Article Snippet: .. The fluorescently labeled DNA was hybridized with GeoChip 5.0 on a MAUI hybridization system (BioMicro Systems, UT, USA) at 65°C for 12 h. The microarrays were scanned using a ScanArray 5000 microarray analysis system (PerkinElmer, Wellesley, MA, USA) at 95% laser power and 68% photomultiplier tube gain. .. Scanned images were saved in 16-bit TIFF format, quantified using ImaGene version 6.0 (BioDiscovery, Inc., Los Angeles, CA, USA), and processed in the Microarray Data Manager system at the Institute for Environmental Genomics website ( http://ieg.ou.edu/microarray ).

Article Title: Stimulation of soil microbial functioning by elevated CO2 may surpass effects mediated by irrigation in a semiarid grassland
Article Snippet: The functioning of microbial communities in response to elevated atmospheric carbon dioxide (eCO2) is one of the most important, but poorly understood, contributors to climate change feedbacks.. The effect of soil moisture caused by eCO2 on soil microbial communities and functioning may be of high importance in water limited ecosystems.. To address this knowledge gap, we measured the functional composition, structure, and functional processes of soil microbial communities under eCO2 and an irrigation treatment simulating water availability with eCO2 at two soil depths (0–5 cm and 5–15 cm) in a mixed-grass semiarid prairie.

Article Title: Functional Overlap between eIF4G Isoforms in Saccharomyces cerevisiae
Article Snippet: Remaining RNA was acid hydrolysed and cDNA purified using Qiagen MinElute columns. .. The resulting cDNA was labeled with Cy3 or Cy5 (GE healthcare PA23001 or PA25001) via the aa-dU nucleoside, and hybridized, using the MAUI hybridization system (BioMicro), to custom cDNA microarrays containing probes for every Saccharomyces Genome Database (SGD)-annotated open reading frame as well as several non-coding regions. ..

Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs.
Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90 ̊C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65 ̊C using the MAUI hybridization system (BioMicro) for 12– 16 hours. ..

Article Title: Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection
Article Snippet: .. Labeled samples (1.5 μg) were hybridized at 42°C to B. melitensis (Nimblegen A4357-001-01) or custom designed arrays (Nimblegen), using a 1× mixing chamber (Nimblegen) and Maui hybridization system (BioMicro). ..

Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs
Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90°C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65°C using the MAUI hybridization system (BioMicro) for 12–16 hours. ..

Labeling:

Article Title: Soil pH Filters the Association Patterns of Aluminum-Tolerant Microorganisms in Rice Paddies
Article Snippet: .. The fluorescently labeled DNA was hybridized with GeoChip 5.0 on a MAUI hybridization system (BioMicro Systems, UT, USA) at 65°C for 12 h. The microarrays were scanned using a ScanArray 5000 microarray analysis system (PerkinElmer, Wellesley, MA, USA) at 95% laser power and 68% photomultiplier tube gain. .. Scanned images were saved in 16-bit TIFF format, quantified using ImaGene version 6.0 (BioDiscovery, Inc., Los Angeles, CA, USA), and processed in the Microarray Data Manager system at the Institute for Environmental Genomics website ( http://ieg.ou.edu/microarray ).

Article Title: Stimulation of soil microbial functioning by elevated CO2 may surpass effects mediated by irrigation in a semiarid grassland
Article Snippet: The functioning of microbial communities in response to elevated atmospheric carbon dioxide (eCO2) is one of the most important, but poorly understood, contributors to climate change feedbacks.. The effect of soil moisture caused by eCO2 on soil microbial communities and functioning may be of high importance in water limited ecosystems.. To address this knowledge gap, we measured the functional composition, structure, and functional processes of soil microbial communities under eCO2 and an irrigation treatment simulating water availability with eCO2 at two soil depths (0–5 cm and 5–15 cm) in a mixed-grass semiarid prairie.

Article Title: Functional Overlap between eIF4G Isoforms in Saccharomyces cerevisiae
Article Snippet: Remaining RNA was acid hydrolysed and cDNA purified using Qiagen MinElute columns. .. The resulting cDNA was labeled with Cy3 or Cy5 (GE healthcare PA23001 or PA25001) via the aa-dU nucleoside, and hybridized, using the MAUI hybridization system (BioMicro), to custom cDNA microarrays containing probes for every Saccharomyces Genome Database (SGD)-annotated open reading frame as well as several non-coding regions. ..

Article Title: Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection
Article Snippet: .. Labeled samples (1.5 μg) were hybridized at 42°C to B. melitensis (Nimblegen A4357-001-01) or custom designed arrays (Nimblegen), using a 1× mixing chamber (Nimblegen) and Maui hybridization system (BioMicro). ..

Microarray:

Article Title: Soil pH Filters the Association Patterns of Aluminum-Tolerant Microorganisms in Rice Paddies
Article Snippet: .. The fluorescently labeled DNA was hybridized with GeoChip 5.0 on a MAUI hybridization system (BioMicro Systems, UT, USA) at 65°C for 12 h. The microarrays were scanned using a ScanArray 5000 microarray analysis system (PerkinElmer, Wellesley, MA, USA) at 95% laser power and 68% photomultiplier tube gain. .. Scanned images were saved in 16-bit TIFF format, quantified using ImaGene version 6.0 (BioDiscovery, Inc., Los Angeles, CA, USA), and processed in the Microarray Data Manager system at the Institute for Environmental Genomics website ( http://ieg.ou.edu/microarray ).

other:

Article Title: Phylogenetic Patterns in the Microbial Response to Resource Availability: Amino Acid Incorporation in San Francisco Bay
Article Snippet: Reagents for synthesis were delivered through an Expedite system (PerSeptive Biosystems, Framingham, MA, USA).

Incubation:

Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs.
Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90 ̊C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65 ̊C using the MAUI hybridization system (BioMicro) for 12– 16 hours. ..

Article Title: Depot-specific mRNA expression programs in human adipocytes suggest physiological specialization via distinct developmental programs
Article Snippet: Differentially dye-labeled sample and reference RNA samples were combined, fragmented (Ambion Cat# 8740), then diluted into in a 50 μl solution containing 3X SSC, 25 mM Hepes-NaOH (pH 7.0), 20 μg of human Cot-1 DNA (Invitrogen Cat# 15279011), 20 μg of poly(A) RNA (Sigma Cat# P9403), 25 μg of yeast tRNA (Invitrogen Cat# 15401029), and 0.3% SDS. .. The sample was incubated at 90°C for 5 min, spun at 14,000 rpm for 10 min in a microcentrifuge, then hybridized at 65°C using the MAUI hybridization system (BioMicro) for 12–16 hours. ..

Mixing Chamber:

Article Title: Differential Expression of Iron Acquisition Genes by Brucella melitensis and Brucella canis during Macrophage Infection
Article Snippet: .. Labeled samples (1.5 μg) were hybridized at 42°C to B. melitensis (Nimblegen A4357-001-01) or custom designed arrays (Nimblegen), using a 1× mixing chamber (Nimblegen) and Maui hybridization system (BioMicro). ..



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BioMicro Systems Inc microarray user interface hybridization chamber
qRT-PCR primers used to validate the <t> microarray </t> results
Microarray User Interface Hybridization Chamber, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+user+interface+hybridization+chamber/micro+array+user+interface+hybridization+system/pmc04811642-164-23-29
Average 90 stars, based on 1 article reviews
microarray user interface hybridization chamber - by Bioz Stars, 2026-11
90/100 stars
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qRT-PCR primers used to validate the  microarray  results

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR primers used to validate the microarray results

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Microarray

qRT-PCR validation of  microarray  data

Journal: Microbiology

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

doi: 10.1099/mic.0.060236-0

Figure Lengend Snippet: qRT-PCR validation of microarray data

Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Techniques: Biomarker Discovery, Microarray