phospho-specific antibody microarray slide (Full Moon BioSystems)

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Full Moon BioSystems phospho-specific antibody microarray slide

Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody <t>microarray</t> assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.

Phospho Specific Antibody Microarray Slide, supplied by Full Moon BioSystems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+slides/antibody+microarray+slide/pmc06963381-315-39-43
Average 90 stars, based on 1 article reviews

phospho-specific antibody microarray slide - by Bioz Stars, 2026-10

90/100 stars

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1) Product Images from "Annona atemoya leaf extract ameliorates cognitive impairment in amyloid-β injected Alzheimer’s disease-like mouse model"

Article Title: Annona atemoya leaf extract ameliorates cognitive impairment in amyloid-β injected Alzheimer’s disease-like mouse model

Journal: Experimental Biology and Medicine

doi: 10.1177/1535370219886269

Figure Legend Snippet: Molecular mechanisms responsible for anti-AD effects of AAL extract. (a) HT22 cells were exposed to H2O2 in the absence or presence of AAL extract (50 μg/mL) for 6 h. Antibody microarray assay was performed using the phospho-specific antibody microarray slide (Full Moon BioSystems). For data acquisition, GenePix 4100A scanner (Axon Instrument, USA) was used. The normalization data were analyzed using Genowiz 4.0™ (Ocimum Biosolutions). The phosphorylation ratio was calculated and represented as fold changes of indicated phosphoproteins after H2O2 treatment normalized to total protein expression (upper panel). Total protein quantification is shown (lower panel). (b) HT22 cells were exposed to H2O2 with or without various concentrations of AAL extract (0, 12.5, 25, or 50 μg/mL) for 6 h. Cell lysates were prepared from HT22 cells and equal amounts of protein were subjected to Western blotting using anti-phospho-CaMK2 β/ν/δ, GRK2, EGFR, Myc, FER, caveolin-1, NFκB p65, and MLRN 2 antibodies to validate the Ab microarray. (c) Protein extracts were prepared from hippocampal tissues in an Aβ-induced AD mouse model. Vehicle or various concentrations of AAL extract (50, 100, or 200 mg/kg) were administered to Aβ mice for 23 days. Western blotting was performed for anti-phospho-EGFR and phospho-GRK2. The validity of the two phospho-antibodies was determined using pre-stained protein marker (Bio-Rad). GAPDH was used as an internal control. Shown blots are representative results from three independent experiments.

Techniques Used: Microarray, Phospho-proteomics, Expressing, Western Blot, Staining, Marker, Control

Microarray:

Article Title: β-Lapachone Exerts Anticancer Effects by Downregulating p53, Lys-Acetylated Proteins, TrkA, p38 MAPK, SOD1, Caspase-2, CD44 and NPM in Oxaliplatin-Resistant HCT116 Colorectal Cancer Cells
Article Snippet: .. The antibody microarray slide (Fullmoon biosystems, Sunnyvale, CA, USA) was treated with 30 mL of blocking solution in a Petri dish, incubated on a shaker at 60 rpm for 30 min at RT and washed with distilled water three times. ..

Article Title: Functional study of acetaldehyde dehydrogenase 1 (ALDH1) in keratinocytes: microarray integrating bioinformatics approaches.
Article Snippet: The function of acetaldehyde dehydrogenase 1 (ALDH1) has been gradually elucidated in several diseases, especially in various cancers.. However, the role of ALDH1 in skin-related diseases has been mostly unknown.. Previously, we found that ALDH1 is involved in the pathogenesis of atopic dermatitis (AD).

Article Title: A phase I/ II study of ivaltinostat combined with gemcitabine and erlotinib in patients with untreated locally advanced or metastatic pancreatic adenocarcinoma
Article Snippet: Protein was extracted from plasma using a protein extraction buffer (Fullmoon Biosystems, Sunnyvale, California) and protein expression was analyzed using antibody microarray analysis (Fullmoon Biosystems) according to the manufacturer's protocol. .. Briefly, 50 μg of protein sample was labeled and incubated with coupling mixture on the antibody microarray slide (Fullmoon Biosystems) and detected with Cy3‐streptavidin (GE Healthcare, Chalfont St. Giles, UK). .. The slide was rinsed scanned using the GenePix 4100A scanner (Axon Instrument, USA) at 10 μm resolution, optimal laser power and PMT.

Article Title: The soluble VCAM-1 level is a potential biomarker predicting severe acute graft versus host disease after allogeneic hematopoietic cell transplantation.
Article Snippet: .. Then, the samples which were incubated for 90 min at room temperature were mixed with 35 μl of stop reagent and incubated at room temperature for 30 min. 30 ml of blocking solution in a petri dish was added to each antibody microarray slide (Full Moon Biosystems, Sunnyvale, CA, USA), which was incubated on a shaker at 60 rpm for 30 min at room temperature, and washed with distilled water. ..

Article Title: TIPRL Regulates Stemness and Survival in Lung Cancer Stem Cells through CaMKK2-CaMK4-CREB Feedback Loop Activation.
Article Snippet: .. The antibody microarray slide (Full Moon Biosystems) was treated with 30 mL of blocking solution in a Petri dish, incubated on a shaker at 60 rpm for 30 min at room temperature, and washed with distilled water. ..

Article Title: Efficacy of GV1001 with gemcitabine/capecitabine in previously untreated patients with advanced pancreatic ductal adenocarcinoma having high serum eotaxin levels (KG4/2015): an open-label, randomised, Phase 3 trial
Article Snippet: The protein was extracted from plasma using a protein extraction buffer (Fullmoon biosystems, Sunnyvale, CA) and protein expression was analysed using antibody microarray analysis (Fullmoon biosystems) according to the manufacturer’s protocol. .. In brief, 50 μg of the protein sample was labelled and incubated with a coupling mixture on the antibody microarray slide (Fullmoon biosystems) and detected with Cy3-streptavidin (GE Healthcare, Chalfont St. Giles, UK). .. The slide was rinsed and scanned using GenePix 4100 A (Axon Instrument, USA) at a 10 μm resolution, optimal laser power, and PMT.

Article Title: Exploring the therapeutic potential of Potentilla fragarioides var. major (Rosaceae) extract in Alzheimer's disease using in vitro and in vivo models: A multi-faceted approach.
Article Snippet: Alzheimer’s disease (AD) is the most common cause of dementia and is caused by various factors including amyloid-beta (Aβ) aggregation.. We investigated the pharmacological effects of the ethanol extract of Potentilla fragarioides var. major (Rosaceae) (EEPF) on AD-related pathogenesis, which remain elusive.. We observed the effects of EEPF on Aβ disaggregation and free-radical scavenging activities for 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) using in vitro assays, evaluated the effects of EEPF on memory loss in two animal models, and examined the molecular regulatory mechanisms of EEPF using an antibody-protein microarray in EEPF-treated neuronal cell lines.

Blocking Assay:

Incubation:

Labeling:

other:

Article Title: CXCR6-CXCL16 Axis Promotes Breast Cancer by Inducing Oncogenic Signaling
Article Snippet: Protein lysates were processed and probed onto cancer signaling phospho-antibody microarray slides as per the manufacturer’s protocol (Fullmoon Biosystems Inc., Sunnyvale, CA, USA).

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