construction of tissue microarrays  (Shanghai Biochip Co. Ltd)

Journal: Cell Reports Medicine

Article Title: HOXC6 drives a therapeutically targetable pancreatic cancer growth and metastasis pathway by regulating MSK1 and PPP2R2B

doi: 10.1016/j.xcrm.2023.101285

Figure Lengend Snippet:

Article Snippet: Tissue microarray slide (PDAC and matched normal pancreas tissue) , US Biomax, Inc. , Cat# PA601.

Techniques: Western Blot, Immunohistochemistry, Immunoprecipitation, Microarray, Recombinant, Transfection, Plasmid Preparation, Membrane, Staining, Flow Cytometry, Viability Assay, Chromatin Immunoprecipitation, shRNA, Sequencing, Binding Assay, Software, Expressing

Journal: Cell Death Discovery

Article Title: Stanniocalcin 2 drives malignant transformation of human glioblastoma cells by targeting SNAI2 and Matrix Metalloproteinases

doi: 10.1038/s41420-022-01090-6

Figure Lengend Snippet: a , b Representative tissue microarray data of STC2 in clinical specimens ( a , GL2082; b , GL208, Biomax). The scale bar represents 100 µm. STC2 staining score from 0-4 were analyzed in different tissues. N, normal tissues; A, astrocytoma; GBM, glioblastoma. One-way ANOVA, * p < 0.05, ** p < 0.01, *** p < 0.001, n.s., no significance. c The levels of STC2 mRNA expression in low grade glioma (LGG) versus glioblastoma (GBM) tissues obtained from TCGA Research Network were analyzed. Unpaired t-test, ** p < 0.01. d The mRNA expression of STC2 in various glioblastoma cell lines were analyzed. After normalizing with 18 S rRNA in each sample, the relative mRNA levels were calculated using A172 = 1. Means ± SD; n = 3 biological replicates. Oneway ANOVA, ** p < 0.01, *** p < 0.001, n.s., no significance. e , f Representative images of Western blotting against STC2 and β-ACTIN in whole lysates of glioblastoma cell lines ( e ) and concentrated culture media (Conditioned media CM) ( f ).

Article Snippet: Brain primary tumor tissue microarray slides (Cat# GL208 and GL2082) were purchased from US Biomax, Inc. (MD, USA).

Techniques: Microarray, Staining, Expressing, Western Blot

Journal: The Journal of Biological Chemistry

Article Title: Peroxiredoxin IV plays a critical role in cancer cell growth and radioresistance through the activation of the Akt/GSK3 signaling pathways

doi: 10.1016/j.jbc.2022.102123

Figure Lengend Snippet: Examination of Prx4 protein levels in patient specimens of prostate cancer by tissue microarray or established prostate cancer cell lines by Western blotting. A , tissue microarray slides and representative images of anti-Prx4 staining of prostate normal and adenocarcinoma. Positive anti-Prx4 staining shows in dark brown and counter staining for nuclear shows light blue . The scale bars represent 5 mm (whole slide), 500 μm (individual tumor), and 100 μm (zoomed in). B and C , anti-Prx4 staining intensity were quantitatively scored by board-certified pathologist and data were compared between prostate normal (n = 50) and adenocarcinoma (n = 66) ( B ), as well as between prostate normal and cancer with different levels of Gleason grading ( C ). D , examination of Prx4 levels in prostate derived nontumorigenic or tumor cell lines by Western blotting. The bar graph on the right shows quantitative data from three independent blots. ∗ p < 0.05 by two-way ANOVA. Prx, peroxiredoxin.

Article Snippet: Human prostate normal and prostate cancer tissue microarray slides were commercially obtained, including BNS19011 and PR803d (US Biomax).

Techniques: Microarray, Western Blot, Staining, Derivative Assay

Journal: Journal of Personalized Medicine

Article Title: Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

doi: 10.3390/jpm12020201

Figure Lengend Snippet: RAD51AP1 protein expression in ovarian cancer (OvCa) patients. A tissue microarray containing 90 unique OvCa patient samples and 10 normal adjacent tissues (NAT) was stained with RAD51AP1 antibody using immunohistochemistry (IHC). Staining intensity values measure the level of RAD51AP1 staining (brown): 0 ≤ 10% stained, 1 = 10–25% stained, 2 = 25–30% stained, 3 = 50–75% stained and 4 ≥ 75%. The cohorts were grouped based on: ( a ) pathological diagnosis: high grade serous (HGS), low grade serous (LGS), clear cell carcinoma (CCC), mucinous (MUC) OvCa and NAT, ( b ) lymph node (LN) metastasis compared to ovarian cancer (OvCa), ( c ) age, and ( d ) early (I and II) and late (III and IV) stages. The images of the cores that are presented above the plots are representative of the group and were taken at 10× magnification. Presented p -values were obtained using ANOVA and t -Test respectively; * p < 0.05, ** p < 0.001.

Article Snippet: IHC was performed on a paraffin-embedded tissue microarray slide (BC11115d, US Biomax Inc., Derwood, MD 20855, USA), containing 100 cores (90 OvCa and 10 normal adjacent ovarian tissue samples abbreviated to NAT; ).

Techniques: Expressing, Microarray, Staining, Immunohistochemistry

Journal: Journal of Personalized Medicine

Article Title: Differential Expression of RAD51AP1 in Ovarian Cancer: Effects of siRNA In Vitro

doi: 10.3390/jpm12020201

Figure Lengend Snippet: Validation of microarray data using RT-qPCR. ( a ) Up-regulation of expression in three of the four genes, with IL1A showing statistical significance (* p = 0.0175) and no change seen in F2RL2. ( b ) Down-regulation was noted in two out of three genes tested (MEGF6 and CTFG, but not SHISA2), with CTFG being significantly reduced in expression (** p = 0.0098). IL1A: interleukin 1 alpha, EGR1: early growth response 1, IL1B: interleukin 1 beta, F2RL2: coagulation factor II thrombin receptor like 2, MEGF: multiple EGF like domains 6, SHISA2: Shisa family member 2, CTGF: connective tissue growth factor.

Article Snippet: IHC was performed on a paraffin-embedded tissue microarray slide (BC11115d, US Biomax Inc., Derwood, MD 20855, USA), containing 100 cores (90 OvCa and 10 normal adjacent ovarian tissue samples abbreviated to NAT; ).

Techniques: Microarray, Quantitative RT-PCR, Expressing, Coagulation

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: USP2 protein expression levels were reduced in HCC-T tissues from HCC patients. (A) Representative total USP2 protein staining with goat anti-human USP2 IgG in the tissues of human normal liver (n=76), HCC-NT (n=38) and HCC-T (n=115) by IHC. As negative controls, human normal liver tissues (n=6) were also stained with goat pre-immune serum. (B) Quantification of the USP2 protein levels were performed with ImageJ. (C) Representative USP2 protein staining in four paired HCC-T and HCC-NT tissues (n=10) by IHC. (D) USP2 protein levels in ten paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Staining

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: USP2 mRNA expression levels were decreased in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (C) individual pairs (n=8). *P<0.05 and **P<0.01 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Real-time Polymerase Chain Reaction

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: Dysregulation of Usp2 mRNA expression in HCC-T tissues from HCC mice. A. The expression levels of Usp2 mRNA in mouse normal liver (n=16), HCC-NT (n=15) and HCC-T (n=15) samples. B. The expression levels of USP2 mRNA in paired HCC-T and HCC-NT tissues. **P<0.01 and ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: USP2b mRNA expression levels were markedly reduced in HCC-T tissues from HCC patients. (A) The expression levels of USP2 mRNA in human normal liver (n=12), HCC-NT (n=8) and HCC-T (n=21) detected by real-time PCR. (B) USP2b protein detected by Western blot in normal liver (n=8), HCC-NT (n=8) and HCC-T tissues (n=8). (C) Quantification of the USP2b protein levels in (B) by ImageJ. (D) The expression levels of USP2 mRNA in paired HCC-NT and HCC-T tissues as groups as well as (E) individual pairs (n=8). ***P<0.001 in students’ t-test for pair-wise comparison or one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: American Journal of Cancer Research

Article Title: Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma

doi:

Figure Lengend Snippet: Usp2b mRNA expression levels were significantly deceased in HCC-T tissues from HCC mice. (A) The expression levels of Usp2b mRNA in mouse normal liver (n=16), HCC-NT (n=26) and HCC-T (n=26) samples. (B) The expression levels of Usp2b mRNA in paired HCC-T and HCC-NT tissues (n=26). (C) Representative Western blots of Usp2b protein in normal liver (n=18), HCC-NT (n=18) and HCC-T tissues (n=17). (D) Quantification of Usp2b protein in (C) by ImageJ. *P<0.05, **P<0.01 and ***P<0.001 in one-way ANOVA, followed by Tukey post-hoc test for multiple comparisons.

Article Snippet: Human liver samples Human liver tissue microarray slides including a total of 76 normal, 38 HCC-NT and 115 HCC-T samples with 10 pairs of HCC-NT and HCC-T samples were purchased from US Biomax (Cat# BC03119b-SC2, LV241a-D024, LVN241-T202 and LVN801-H139).

Techniques: Expressing, Western Blot