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cdna microarray analysis service  (Genome Systems Inc)

 
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    Genome Systems Inc cdna microarray analysis service
    Cdna Microarray Analysis Service, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+service/cdna+microarray+analysis+service/pm10799550-71-17-25
    Average 90 stars, based on 1 article reviews
    cdna microarray analysis service - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Purification:

    Article Title: Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction.
    Article Snippet: Poly(A) RNA was purified by a double pass over Oligo Tex mRNA isolation columns as described in manufacturer’s technical bulletin (Qiagen, Valencia, CA). .. Eight hundred ng of highly purified poly(A) RNA from the control and FVIIatreated cells were sent for cDNA microarray analysis service (UniGEM Human V microarray, Genome Systems Inc, St. Louis, MO). ..

    Control:

    Article Title: Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction.
    Article Snippet: Poly(A) RNA was purified by a double pass over Oligo Tex mRNA isolation columns as described in manufacturer’s technical bulletin (Qiagen, Valencia, CA). .. Eight hundred ng of highly purified poly(A) RNA from the control and FVIIatreated cells were sent for cDNA microarray analysis service (UniGEM Human V microarray, Genome Systems Inc, St. Louis, MO). ..

    Microarray:

    Article Title: Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction.
    Article Snippet: Poly(A) RNA was purified by a double pass over Oligo Tex mRNA isolation columns as described in manufacturer’s technical bulletin (Qiagen, Valencia, CA). .. Eight hundred ng of highly purified poly(A) RNA from the control and FVIIatreated cells were sent for cDNA microarray analysis service (UniGEM Human V microarray, Genome Systems Inc, St. Louis, MO). ..



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    CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

    Journal: Frontiers in Immunology

    Article Title: Altered circular RNA expressions in extracellular vesicles from bronchoalveolar lavage fluids in mice after bacterial infections

    doi: 10.3389/fimmu.2024.1354676

    Figure Lengend Snippet: CircRNA profiles in BALF EVs, serum EVs and BALF cells. Three mice were used in this experiment. BALF and serum were collected and pooled after exposure to PBS or LPS (1µg/mouse) or HCl (0.1N) for 24h. EVs were separated using ultracentrifugation as previously described . RNA was isolated from the EVs and subjected to circRNA microarrays by Arraystar Inc (Rockville, MD). Heatmap of the circRNA arrays in EVs obtained from BALF in the absence and presence of noxious stimuli. (A) BALF EVs obtained from mice that were exposed to PBS or LPS. (B) BALF EVs obtained from mice that were exposed to PBS or HCL. (C) the changes of EV-cargo circRNA profiles between non-infectious stimuli (HCL) and endotoxin (LPS) were expressed in Log2 fold change. (D) A representative picture showing the histological changes was presented here. The inflammatory score was determined by a double-blinded examiner using five lung sections per mouse, and three mice per treatment. The mean value is graphed and presented. ***p<0.05.

    Article Snippet: For the circRNA profiling using microarrays, we used the service from Arraystar (Rockville, MD).

    Techniques: Isolation