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microgrid arrayer  (BioRobotics Ltd)

 
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    BioRobotics Ltd microgrid arrayer
    Microgrid Arrayer, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+printer+biorobotics+microgrid+ii/microgrid+total+array+system/pm12028573-233-6-5
    Average 90 stars, based on 1 article reviews
    microgrid arrayer - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    other:

    Article Title: Development and application of a microarray meter tool to optimize microarray experiments
    Article Snippet: This data set revealed a similar performance between the QArrayMini/ChipMaker and the BioRobotics MicroGrid II/MicroSpot 10 K arrays as regards the dynamic range performance of the microarrays.

    Amplification:

    Article Title: Gene Expression Profiling Identifies FKBP39 as an Inhibitor of Autophagy in Larval Drosophila Fat Body
    Article Snippet: .. Briefly, 3200 amplified cDNA inserts from Drosophila melanogaster were purified with MultiScreen-PCR plate (Millipore), resuspended in 50% dimethylsulfoxide/water, and arrayed on amino-silanized slides (Sigma-Aldrich) by using a MicroGrid Total Array System (BioRobotics) spotter with 16 pins with a 4×4 format. ..

    Article Title: Functional genomics of calcium channels in human melanoma cells.
    Article Snippet: .. Construction and use of microarrays were performed as described.28 Briefly, 2,215 cDNA inserts from human cDNA libraries were amplified and arrayed in duplicate on cDNA slides (Full Moon BioSystems, Sunnyvale, CA) by using a MicroGrid Total Array System (BioRobotics, Cambridge, UK) spotter (spot size 5 200 lm). ..

    Purification:

    Article Title: Gene Expression Profiling Identifies FKBP39 as an Inhibitor of Autophagy in Larval Drosophila Fat Body
    Article Snippet: .. Briefly, 3200 amplified cDNA inserts from Drosophila melanogaster were purified with MultiScreen-PCR plate (Millipore), resuspended in 50% dimethylsulfoxide/water, and arrayed on amino-silanized slides (Sigma-Aldrich) by using a MicroGrid Total Array System (BioRobotics) spotter with 16 pins with a 4×4 format. ..

    Article Title: Pattern Triggered Immunity (PTI) in Tobacco: Isolation of Activated Genes Suggests Role of the Phenylpropanoid Pathway in Inhibition of Bacterial Pathogens
    Article Snippet: .. PCR products from the cDNA clones were purified with MultiScreen-PCR plate (Millipore), resuspended in 50% dimethylsulfoxide/water, and arrayed on FMB cDNA slides (Full Moon BioSystems) using a MicroGrid Total Array System (BioRobotics) spotter with 16 pins in a 4×4 grid format. cDNA elements were deposited in duplicates. .. After printing, DNA was UV crosslinked to the slides with 700 mJ energy (Stratalinker, Stratagene, La Jolla, CA).

    Microarray:

    Article Title: A novel monoclonal antibody targeting carboxymethyllysine, an advanced glycation end product in atherosclerosis and pancreatic cancer
    Article Snippet: .. Microarray printing was performed as earlier described [ – ] on hydrogel coated Nexterion ® Slides H, NHS using a MicroGrid array printer (60 nL/deposit, quilled pins, 250 μm pitch, BioRobotics, Genomics Solutions, Cambridgeshire, UK) with TAS Application suite software. ..

    Software:

    Article Title: A novel monoclonal antibody targeting carboxymethyllysine, an advanced glycation end product in atherosclerosis and pancreatic cancer
    Article Snippet: .. Microarray printing was performed as earlier described [ – ] on hydrogel coated Nexterion ® Slides H, NHS using a MicroGrid array printer (60 nL/deposit, quilled pins, 250 μm pitch, BioRobotics, Genomics Solutions, Cambridgeshire, UK) with TAS Application suite software. ..

    Polymerase Chain Reaction:

    Article Title: Pattern Triggered Immunity (PTI) in Tobacco: Isolation of Activated Genes Suggests Role of the Phenylpropanoid Pathway in Inhibition of Bacterial Pathogens
    Article Snippet: .. PCR products from the cDNA clones were purified with MultiScreen-PCR plate (Millipore), resuspended in 50% dimethylsulfoxide/water, and arrayed on FMB cDNA slides (Full Moon BioSystems) using a MicroGrid Total Array System (BioRobotics) spotter with 16 pins in a 4×4 grid format. cDNA elements were deposited in duplicates. .. After printing, DNA was UV crosslinked to the slides with 700 mJ energy (Stratalinker, Stratagene, La Jolla, CA).

    Clone Assay:

    Article Title: Pattern Triggered Immunity (PTI) in Tobacco: Isolation of Activated Genes Suggests Role of the Phenylpropanoid Pathway in Inhibition of Bacterial Pathogens
    Article Snippet: .. PCR products from the cDNA clones were purified with MultiScreen-PCR plate (Millipore), resuspended in 50% dimethylsulfoxide/water, and arrayed on FMB cDNA slides (Full Moon BioSystems) using a MicroGrid Total Array System (BioRobotics) spotter with 16 pins in a 4×4 grid format. cDNA elements were deposited in duplicates. .. After printing, DNA was UV crosslinked to the slides with 700 mJ energy (Stratalinker, Stratagene, La Jolla, CA).



    Similar Products

    microarray printer biorobotics microgrid ii  (BioRobotics Ltd)
    90
    BioRobotics Ltd microarray printer biorobotics microgrid ii
    Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding <t>microarray</t> for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).
    Microarray Printer Biorobotics Microgrid Ii, supplied by BioRobotics Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/microarray+printer+biorobotics+microgrid+ii/microgrid+ii+microarray+robot/pmc04770247-73-24-26
    Average 90 stars, based on 1 article reviews
    microarray printer biorobotics microgrid ii - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding microarray for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).

    Journal: Nucleic Acids Research

    Article Title: The Effects of Sequence Variation on Genome-wide NRF2 Binding—New Target Genes and Regulatory SNPs

    doi: 10.1093/nar/gkw052

    Figure Lengend Snippet: Testing the limits of NRF2 binding. Systematic multivariate ARE results by protein binding microarray for NRF2-MAFG heterodimer are shown. Tolerance for sequence variation was studied for positions 1–3 (white), 6–8 (grey) and 9–11 (dark grey) ( A ) and positions 1, 5 and 11 (seed sequences are shown in grey) ( B ). Results are depicted as measured binding relative to NQO1.ARE binding (black) (mean ± S.E.M., n = 45).

    Article Snippet: The dilutions were dispensed in a 384-well plate (polypropylene plate No 267462, Nunc, N.Y, USA) and printed onto the avidin-coated glass slides with a microarray printer (BioRobotics MicroGrid II, BioRobotics Ltd, Cambridge, UK).

    Techniques: Binding Assay, Protein Binding, Microarray, Sequencing

    A SNP in FTL promoter has drastic effects of NRF2 binding and transcriptional activation. ( A ) A promoter analysis of the FTL gene at chr19 showing the location of experimentally verified NRF2 binding ARE together with dbSNP (v138) and ENCODE ChIP-seq data. ChIP-seq track displays combined MAFF and MAFK binding signals in H1-hESC (MAFK), K562 (MAFF, MAFK), HeLa-S3 (MAFK), HepG (MAFF, MAFK) and IMR90 (MAFK) cell lines. ( B ) Detailed view showing FTL ARE sequence and the SNP (rs113067944, A→C) position. ( C ) Protein binding microarray results for FTL.ARE.A and the SNP bearing FTL.ARE.C. Results are calculated as measured binding relative to NQO1.ARE binding (mean ± S.E.M, n = 39.). Scramble oligonucleotides served as negative control. ( D ) HEK-293T cells were transfected with NQO1-ARE and FTL-ARE bearing either allele A or allele C with and without NRF2 -expressing plasmids. Twenty-four h after transfection cells were treated with NRF2 inducer (L-SFN) for 16 h followed by luciferase activity measurements. An empty pGL3 promoter vector served as control and activities were normalized to β-galactosidase activity. Results are shown relative to control (mean± S.E.M, n = 4).

    Journal: Nucleic Acids Research

    Article Title: The Effects of Sequence Variation on Genome-wide NRF2 Binding—New Target Genes and Regulatory SNPs

    doi: 10.1093/nar/gkw052

    Figure Lengend Snippet: A SNP in FTL promoter has drastic effects of NRF2 binding and transcriptional activation. ( A ) A promoter analysis of the FTL gene at chr19 showing the location of experimentally verified NRF2 binding ARE together with dbSNP (v138) and ENCODE ChIP-seq data. ChIP-seq track displays combined MAFF and MAFK binding signals in H1-hESC (MAFK), K562 (MAFF, MAFK), HeLa-S3 (MAFK), HepG (MAFF, MAFK) and IMR90 (MAFK) cell lines. ( B ) Detailed view showing FTL ARE sequence and the SNP (rs113067944, A→C) position. ( C ) Protein binding microarray results for FTL.ARE.A and the SNP bearing FTL.ARE.C. Results are calculated as measured binding relative to NQO1.ARE binding (mean ± S.E.M, n = 39.). Scramble oligonucleotides served as negative control. ( D ) HEK-293T cells were transfected with NQO1-ARE and FTL-ARE bearing either allele A or allele C with and without NRF2 -expressing plasmids. Twenty-four h after transfection cells were treated with NRF2 inducer (L-SFN) for 16 h followed by luciferase activity measurements. An empty pGL3 promoter vector served as control and activities were normalized to β-galactosidase activity. Results are shown relative to control (mean± S.E.M, n = 4).

    Article Snippet: The dilutions were dispensed in a 384-well plate (polypropylene plate No 267462, Nunc, N.Y, USA) and printed onto the avidin-coated glass slides with a microarray printer (BioRobotics MicroGrid II, BioRobotics Ltd, Cambridge, UK).

    Techniques: Binding Assay, Activation Assay, ChIP-sequencing, Sequencing, Protein Binding, Microarray, Negative Control, Transfection, Expressing, Luciferase, Activity Assay, Plasmid Preparation, Control