Review




Structured Review

Corning Life Sciences microarray hybridization chamber
Plot of gene expression of sorted/unsorted cells . Plot of one-sample T-test p-values with fold-change in gene expression for all ORFs in <t>microarray</t> study I. Vertical lines show the cutoff of fold-change of 2 (Log 2 ratio of ± 1), while the horizontal line shows the cutoff of p-value 0.05. Genes located in the left-bottom corner (Log 2 ratio <-1 and p-value <0.05) and in the right-bottom corner (Log 2 ratio >1 and p-value <0.05) were considered to have their expressions changed due to dispersion/homogenization and IMS (immuno-magnetic separation) cell sorting. A total of ten genes were selected using these criteria, eight of which also differentially expressed in the independent microarray study II.
Microarray Hybridization Chamber, supplied by Corning Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/microarray+hybridization+chambers/pmc03076228-154-4-8
Average 90 stars, based on 1 article reviews
microarray hybridization chamber - by Bioz Stars, 2026-10
90/100 stars

Images

1) Product Images from "Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis"

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis

Journal: BMC Microbiology

doi: 10.1186/1471-2180-11-59

Plot of gene expression of sorted/unsorted cells . Plot of one-sample T-test p-values with fold-change in gene expression for all ORFs in microarray study I. Vertical lines show the cutoff of fold-change of 2 (Log 2 ratio of ± 1), while the horizontal line shows the cutoff of p-value 0.05. Genes located in the left-bottom corner (Log 2 ratio <-1 and p-value <0.05) and in the right-bottom corner (Log 2 ratio >1 and p-value <0.05) were considered to have their expressions changed due to dispersion/homogenization and IMS (immuno-magnetic separation) cell sorting. A total of ten genes were selected using these criteria, eight of which also differentially expressed in the independent microarray study II.
Figure Legend Snippet: Plot of gene expression of sorted/unsorted cells . Plot of one-sample T-test p-values with fold-change in gene expression for all ORFs in microarray study I. Vertical lines show the cutoff of fold-change of 2 (Log 2 ratio of ± 1), while the horizontal line shows the cutoff of p-value 0.05. Genes located in the left-bottom corner (Log 2 ratio <-1 and p-value <0.05) and in the right-bottom corner (Log 2 ratio >1 and p-value <0.05) were considered to have their expressions changed due to dispersion/homogenization and IMS (immuno-magnetic separation) cell sorting. A total of ten genes were selected using these criteria, eight of which also differentially expressed in the independent microarray study II.

Techniques Used: Expressing, Microarray, Homogenization, FACS

Genes identified as differentially expressed # between IMS sorted E. coli cells versus unsorted E. coli cells* by the method of cDNA  microarray  and their differential expression confirmed with another method of qPCR
Figure Legend Snippet: Genes identified as differentially expressed # between IMS sorted E. coli cells versus unsorted E. coli cells* by the method of cDNA microarray and their differential expression confirmed with another method of qPCR

Techniques Used: Microarray, Expressing

Related Articles

Hybridization:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: Gene expression and pathologic alterations in juvenile rainbow trout due to chronic dietary TCDD exposure
Article Snippet: .. Hybridization was performed using HybriSlips coverslips (Sigma, St. Louis, MO) and Corning microarray hybridization chambers (Corning, Tewksbury, MA) in a water bath at 50°C for 16 h; the first hybridization used LNA dT blocker (vial 9 in the Array 900 kit) and formamide-based hybridization buffer (vial 7 in the Array 900 kit) as directed in the Array 900 manual. .. Following the first hybridization, arrays were washed in 2× SSC (Ambion, Austin, TX), 0.2% SDS solution (Ambion, Austin, TX) at 50°C (1 × 15 min), 2× SSC (1 × 15 min) at room temperature, then 0.2× SSC (1 × 15 min) at room temperature; all microarray wash solutions were made using RNase-free water.

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia
Article Snippet: .. Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA). .. Hybridization and wash buffers from the miRCURY LNA microRNA Array Kit (Exiqon) were used according to the manufacturer’s instructions.

Article Title: Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids
Article Snippet: .. Hybridisation was performed overnight in a 42 °C water bath in Corning microarray hybridization chambers. ..

Article Title: Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates.
Article Snippet: A novel immobilization approach involving binding of preformed streptavidin/biotinylated oligonucleotide conjugates onto surfaces coated with biotinylated bovine serum albumin is presented.. Microarrays prepared according to the proposed method were compared, in terms of detection sensitivity and specificity, with other immobilization schemes employing coupling of biotinylated oligonucleotides onto directly adsorbed surface streptavidin, or sequential coupling of streptavidin and biotinylated oligonucleotides onto a layer of adsorbed biotinylated bovine serum albumin.. A comparison was performed employing biotinylated oligonucleotides corresponding to wildand mutant-type sequences of seven single point mutations of the BRCA1 gene.

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Microarray:

Article Title: Separation of the bacterial species, Escherichia coli , from mixed-species microbial communities for transcriptome analysis
Article Snippet: .. Hybridization was in a Corning Microarray Hybridization Chamber (Corning Inc.) in 42°C water bath. .. Microarray slides were scanned with a Virtek ChipReader (Virtek Vision, Waterloo, ON, Canada).

Article Title: Proteomic Monitoring of B Cell Immunity
Article Snippet: HybriWell gasket adhesive (Grace Bio-Labs, Bend, OR, USA). .. Microarray Hybridization Chamber (Corning, Corning, NY, USA). ..

Article Title: Gene expression and pathologic alterations in juvenile rainbow trout due to chronic dietary TCDD exposure
Article Snippet: .. Hybridization was performed using HybriSlips coverslips (Sigma, St. Louis, MO) and Corning microarray hybridization chambers (Corning, Tewksbury, MA) in a water bath at 50°C for 16 h; the first hybridization used LNA dT blocker (vial 9 in the Array 900 kit) and formamide-based hybridization buffer (vial 7 in the Array 900 kit) as directed in the Array 900 manual. .. Following the first hybridization, arrays were washed in 2× SSC (Ambion, Austin, TX), 0.2% SDS solution (Ambion, Austin, TX) at 50°C (1 × 15 min), 2× SSC (1 × 15 min) at room temperature, then 0.2× SSC (1 × 15 min) at room temperature; all microarray wash solutions were made using RNase-free water.

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Article Title: Overexpression of primary microRNA 221/222 in acute myeloid leukemia
Article Snippet: .. Arrays were hybridized overnight at 60°C in microarray hybridization chambers (Corning, Corning, NY, USA). .. Hybridization and wash buffers from the miRCURY LNA microRNA Array Kit (Exiqon) were used according to the manufacturer’s instructions.

Article Title: Stable Membrane-Association of mRNAs in Etiolated, Greening and Mature Plastids
Article Snippet: .. Hybridisation was performed overnight in a 42 °C water bath in Corning microarray hybridization chambers. ..

Article Title: Improved DNA microarray detection sensitivity through immobilization of preformed in solution streptavidin/biotinylated oligonucleotide conjugates.
Article Snippet: A novel immobilization approach involving binding of preformed streptavidin/biotinylated oligonucleotide conjugates onto surfaces coated with biotinylated bovine serum albumin is presented.. Microarrays prepared according to the proposed method were compared, in terms of detection sensitivity and specificity, with other immobilization schemes employing coupling of biotinylated oligonucleotides onto directly adsorbed surface streptavidin, or sequential coupling of streptavidin and biotinylated oligonucleotides onto a layer of adsorbed biotinylated bovine serum albumin.. A comparison was performed employing biotinylated oligonucleotides corresponding to wildand mutant-type sequences of seven single point mutations of the BRCA1 gene.

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..

Labeling:

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Purification:

Article Title: Gene expression profiling of acute myeloid leukemia samples from adult patients with AML-M1 and -M2 through boutique microarrays, real-time PCR and droplet digital PCR
Article Snippet: .. The labeled cDNA was purified (MinElute Reaction Cleanup kit; Qiagen, Hilden, Germany), dissolved in hybridization buffer (5X SSC, 0.1% SDS and 0.1 mg BSA/ml) and kept up to 30 min at 50°C prior to hybridization in Corning microarray hybridization chambers in a HybArray12 (Perkin-Elmer) or a water bath by using a step-down hybridization protocol (5 h/50°C, 5 h/45°C and 5 h/40°C). ..

Incubation:

Article Title: An antibody-based microarray assay for small RNA detection
Article Snippet: .. The RNA solution was confined to an area of 25 × 44 mm by use of an elevated coverslip (LifterSlip, 25X44I-2-4775; Erie Scientific, Portsmouth, NH), and the slide was incubated at 45°C in a microarray hybridization chamber (catalog no. 2551, Corning) for 16 h. The coverslip was removed and the array washed several times by placing it into 50 ml conical tubes containing 40 ml of non-stringent wash buffer (NSWB) [6× SSPE (20× SSPE: 3.6 M NaCl, 0.2 M NaH 2 PO 4 , 0.02 M EDTA, pH 7.4), 0.01% Tween 20]. ..



Similar Products

90
Agilent technologies microarray hybridisation chamber user guide
Microarray Hybridisation Chamber User Guide, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/us11976284-506-7-12
Average 90 stars, based on 1 article reviews
microarray hybridisation chamber user guide - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Agilent technologies microarray hybridisation chamber
Microarray Hybridisation Chamber, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/us11168117-495-7-12
Average 90 stars, based on 1 article reviews
microarray hybridisation chamber - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Agilent technologies hybridisation oven (microarray hybridisation chambers
Hybridisation Oven (Microarray Hybridisation Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/pm33727586-181-5-10
Average 90 stars, based on 1 article reviews
hybridisation oven (microarray hybridisation chambers - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Agilent technologies microarray hybridisation chamber kit
(A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
Microarray Hybridisation Chamber Kit, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/bio_rxiv__2020__12__17__423258-123-26-30
Average 90 stars, based on 1 article reviews
microarray hybridisation chamber kit - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

90
Agilent technologies microarray hybridisation chambers
(A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
Microarray Hybridisation Chambers, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/pm19150360-209-5-8
Average 90 stars, based on 1 article reviews
microarray hybridisation chambers - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier

Image Search Results


(A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

Journal: bioRxiv

Article Title: Genotype-phenotype map of an RNA-ligand complex

doi: 10.1101/2020.12.17.423258

Figure Lengend Snippet: (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

Article Snippet: 40 μl of the hybridisation mix was transferred into each of eight wells on a hybridisation gasket slide (Agilent, G2534-60015) during hybridisation assembly using the Agilent Microarray Hybridisation Chamber Kit (Agilent, Cat No. G2534A) and following the standard protocol.

Techniques: Microarray, Fluorescence, Concentration Assay, Standard Deviation