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BioMicro Systems Inc microarray user interface hybridization chamber
qRT-PCR primers used to validate the <t> microarray </t> results
Microarray User Interface Hybridization Chamber, supplied by BioMicro Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+hybridisation+chamber/micro+array+user+interface+hybridization+system/pmc04811642-164-23-29
Average 90 stars, based on 1 article reviews
microarray user interface hybridization chamber - by Bioz Stars, 2026-10
90/100 stars

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1) Product Images from "Global transcriptional analysis of the stringent response in Enterococcus faecalis"

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis

Journal: Microbiology

doi: 10.1099/mic.0.060236-0

qRT-PCR primers used to validate the  microarray  results
Figure Legend Snippet: qRT-PCR primers used to validate the microarray results

Techniques Used: Microarray

qRT-PCR validation of  microarray  data
Figure Legend Snippet: qRT-PCR validation of microarray data

Techniques Used: Biomarker Discovery, Microarray

Related Articles

Microarray:

Article Title: Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA
Article Snippet: Both cDNAs were purified using a MinElute PCR purification kit (Qiagen) and labeled with Cy5 (sample DNA) or Cy3 (reference RNA) as described previously [ 14 ]. .. The probes were mixed, diluted in 5 μl DMSO plus 1.7 μl 1 M NaHCO 3 , and hybridized to 36 K human 60-mer ODN array slides (NCI Human Array Set Hs-Operon V 3.0) at 42°C for 18 h in a Micro Array User Interface hybridization system (BioMicro Systems, Salt Lake City, UT, USA), followed by washing, centrifugation, and air-drying. .. Analysis of gene expression Arrays were scanned using a GenePix 4000B scanner (Axon Instruments, Union City, CA, USA) and analyzed using the GenePix Pro 6.0 software tool (Axon Instruments), using Gene Array List files provided by the manufacturer at http://madb.nci.nih.gov/ .

Article Title: Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus
Article Snippet: Briefly, cDNA was synthesized from 1.5 μg of total RNA at 42 °C using SS-II reverse transcription enzyme and RT primers specific to cy5/cy3 dye dendrimers (Genisphere Inc.). .. The cDNAs were first hybridized with microarray slides overnight at 42 °C in MAUI microarray hybridization chambers (Biomicro systems Inc.) followed by stringent washes. ..

Article Title: Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA.
Article Snippet: Both cDNAs were purified using a MinElute PCR purification kit (Qiagen) and labeled with Cy5 (sample DNA) or Cy3 (reference RNA) as described previously [14]. .. The probes were mixed, diluted in 5 l DMSO plus 1.7 l 1 M NaHCO3, and hybridized to 36 K human 60-mer ODN array slides (NCI Human Array Set HsOperon V 3.0) at 42°C for 18 h in a Micro Array User Interface hybridization system (BioMicro Systems, Salt Lake City, UT, USA), followed by washing, centrifugation, and air-drying. ..

Hybridization:

Article Title: Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA
Article Snippet: Both cDNAs were purified using a MinElute PCR purification kit (Qiagen) and labeled with Cy5 (sample DNA) or Cy3 (reference RNA) as described previously [ 14 ]. .. The probes were mixed, diluted in 5 μl DMSO plus 1.7 μl 1 M NaHCO 3 , and hybridized to 36 K human 60-mer ODN array slides (NCI Human Array Set Hs-Operon V 3.0) at 42°C for 18 h in a Micro Array User Interface hybridization system (BioMicro Systems, Salt Lake City, UT, USA), followed by washing, centrifugation, and air-drying. .. Analysis of gene expression Arrays were scanned using a GenePix 4000B scanner (Axon Instruments, Union City, CA, USA) and analyzed using the GenePix Pro 6.0 software tool (Axon Instruments), using Gene Array List files provided by the manufacturer at http://madb.nci.nih.gov/ .

Article Title: Differential host gene responses from infection with neurovirulent and partially-neurovirulent strains of Venezuelan equine encephalitis virus
Article Snippet: Briefly, cDNA was synthesized from 1.5 μg of total RNA at 42 °C using SS-II reverse transcription enzyme and RT primers specific to cy5/cy3 dye dendrimers (Genisphere Inc.). .. The cDNAs were first hybridized with microarray slides overnight at 42 °C in MAUI microarray hybridization chambers (Biomicro systems Inc.) followed by stringent washes. ..

Article Title: Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA.
Article Snippet: Both cDNAs were purified using a MinElute PCR purification kit (Qiagen) and labeled with Cy5 (sample DNA) or Cy3 (reference RNA) as described previously [14]. .. The probes were mixed, diluted in 5 l DMSO plus 1.7 l 1 M NaHCO3, and hybridized to 36 K human 60-mer ODN array slides (NCI Human Array Set HsOperon V 3.0) at 42°C for 18 h in a Micro Array User Interface hybridization system (BioMicro Systems, Salt Lake City, UT, USA), followed by washing, centrifugation, and air-drying. ..

Centrifugation:

Article Title: Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA
Article Snippet: Both cDNAs were purified using a MinElute PCR purification kit (Qiagen) and labeled with Cy5 (sample DNA) or Cy3 (reference RNA) as described previously [ 14 ]. .. The probes were mixed, diluted in 5 μl DMSO plus 1.7 μl 1 M NaHCO 3 , and hybridized to 36 K human 60-mer ODN array slides (NCI Human Array Set Hs-Operon V 3.0) at 42°C for 18 h in a Micro Array User Interface hybridization system (BioMicro Systems, Salt Lake City, UT, USA), followed by washing, centrifugation, and air-drying. .. Analysis of gene expression Arrays were scanned using a GenePix 4000B scanner (Axon Instruments, Union City, CA, USA) and analyzed using the GenePix Pro 6.0 software tool (Axon Instruments), using Gene Array List files provided by the manufacturer at http://madb.nci.nih.gov/ .

Article Title: Activation of type I interferon-dependent genes characterizes the "core response" induced by CpG DNA.
Article Snippet: Both cDNAs were purified using a MinElute PCR purification kit (Qiagen) and labeled with Cy5 (sample DNA) or Cy3 (reference RNA) as described previously [14]. .. The probes were mixed, diluted in 5 l DMSO plus 1.7 l 1 M NaHCO3, and hybridized to 36 K human 60-mer ODN array slides (NCI Human Array Set HsOperon V 3.0) at 42°C for 18 h in a Micro Array User Interface hybridization system (BioMicro Systems, Salt Lake City, UT, USA), followed by washing, centrifugation, and air-drying. ..

other:

Article Title: Global transcriptional analysis of the stringent response in Enterococcus faecalis
Article Snippet: The slides were hybridized to a mixture containing equal amounts of test and reference cDNA for 16 h at 42 °C in a MicroArray User Interface (MAUI) hybridization chamber (BioMicro Systems).

Article Title: SMN deficiency alters Nrxn2 expression and splicing in zebrafish and mouse models of spinal muscular atrophy.
Article Snippet: Arrays were hybridized overnight at 428C using Micro Array User Interface (MAUI) hybridization stations (BioMicro).Scanning of the arrayswas performed using the GenePix Pro 4.0 image analysis software on a GenePix 4000B microarray scanner (Axon Instruments, CA, USA).

Article Title: Transcriptome analysis reveals that ClpXP proteolysis controls key virulence properties of Streptococcus mutans
Article Snippet: Mixtures of test and reference cDNA were hybridized to the microarray slides for 16 h at 42 °C in a MAUI (MicroArray User Interface) hybridization chamber (BioMicro Systems).

Article Title: Gene expression of the marine copepod Calanus finmarchicus: Responses to small-scale environmental variation in the Gulf of Maine (NW Atlantic Ocean)
Article Snippet: Contents lists available at SciVerse ScienceDirect Journal of Experimental Marine Biology and Ecology j ourna l homepage: www.e lsev ie r .com/ locate / jembe Gene expression of the marine copepod Calanus finmarchicus: Responses to small-scale environmental variation in the Gulf of Maine (NW Atlantic Ocean) E. Unal a,⁎, A. Bucklin a, P.H.. Lenz b, D.W. Towle c,1 a Department of Marine Sciences, University of Connecticut, 1080 Shennecossett Rd., Groton, CT 06340, USA b Pacific Biosciences Research Center, University of Hawaii at Manoa, Honolulu, HI 96822, USA c Center for Marine Functional Genomics, Mount Desert Island Biological Laboratory, Salisbury Cove, ME 04672, USA ⁎ Corresponding author at: Department of Marine Scien 1080 Shennecossett Rd., Groton, CT 06340, USA.. Fax: +1 E-mail address: ebru.unal@uconn.edu (E. Unal).

Article Title: Whole-Genome Microarray and Gene Deletion Studies Reveal Regulation of the Polyhydroxyalkanoate Production Cycle by the Stringent Response in Ralstonia eutropha H16
Article Snippet: 484 Acknowledgements 485 We thank Dr. Stuart Levine, Ms. Manlin Luo, and the rest of the MIT BioMicro Center for 486 hybridization and processing of microarray samples.



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(A) Correlation of K d (DFHBI-1T) estimates from <t>microarray</t> and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.
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Image Search Results


(A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

Journal: bioRxiv

Article Title: Genotype-phenotype map of an RNA-ligand complex

doi: 10.1101/2020.12.17.423258

Figure Lengend Snippet: (A) Correlation of K d (DFHBI-1T) estimates from microarray and spectrofluorometric measurements for five selected variants. (B-D) Fluorescence of indicated Broccoli variants as a function of DFHBI-1T, Mg 2+ and K + concentration. Error bars represent standard deviation from 4 experimental replicates.

Article Snippet: 40 μl of the hybridisation mix was transferred into each of eight wells on a hybridisation gasket slide (Agilent, G2534-60015) during hybridisation assembly using the Agilent Microarray Hybridisation Chamber Kit (Agilent, Cat No. G2534A) and following the standard protocol.

Techniques: Microarray, Fluorescence, Concentration Assay, Standard Deviation