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Genome Systems Inc cdna microarray analysis service
Cdna Microarray Analysis Service, supplied by Genome Systems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/microarray+analysis+service/cdna+microarray+analysis+service/pm10799550-71-17-25
Average 90 stars, based on 1 article reviews
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Purification:

Article Title: Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction.
Article Snippet: Poly(A) RNA was purified by a double pass over Oligo Tex mRNA isolation columns as described in manufacturer’s technical bulletin (Qiagen, Valencia, CA). .. Eight hundred ng of highly purified poly(A) RNA from the control and FVIIatreated cells were sent for cDNA microarray analysis service (UniGEM Human V microarray, Genome Systems Inc, St. Louis, MO). ..

Control:

Article Title: Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction.
Article Snippet: Poly(A) RNA was purified by a double pass over Oligo Tex mRNA isolation columns as described in manufacturer’s technical bulletin (Qiagen, Valencia, CA). .. Eight hundred ng of highly purified poly(A) RNA from the control and FVIIatreated cells were sent for cDNA microarray analysis service (UniGEM Human V microarray, Genome Systems Inc, St. Louis, MO). ..

Microarray:

Article Title: Factor VIIa and thrombin induce the expression of Cyr61 and connective tissue growth factor, extracellular matrix signaling proteins that could act as possible downstream mediators in factor VIIa x tissue factor-induced signal transduction.
Article Snippet: Poly(A) RNA was purified by a double pass over Oligo Tex mRNA isolation columns as described in manufacturer’s technical bulletin (Qiagen, Valencia, CA). .. Eight hundred ng of highly purified poly(A) RNA from the control and FVIIatreated cells were sent for cDNA microarray analysis service (UniGEM Human V microarray, Genome Systems Inc, St. Louis, MO). ..



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Pathway analysis comparing treatment groups. Ingenuity Pathway Analysis was used to predict signaling pathways between Arrest and Sham mice (top), Sham+Metformin (Sham+Met) and Sham mice (middle), and Arrest+Metformin (Arrest+Met) to Arrest mice (bottom) based on microarray data collected from left ventricles one day after arrest (n = 6/group). Bar-graphs include significance values for pathway changes (−log(p-value)) and are colored according to z-score to predict directionality of changes. Differentially expressed genes were included in the analysis when p < 0.05 based on microarray data. Principal component analysis, volcano plots, and hierarchical clustering are included in .

Journal: Journal of molecular and cellular cardiology plus

Article Title: Metformin preconditioning protects against myocardial stunning and preserves protein translation in a mouse model of cardiac arrest

doi: 10.1016/j.jmccpl.2023.100034

Figure Lengend Snippet: Pathway analysis comparing treatment groups. Ingenuity Pathway Analysis was used to predict signaling pathways between Arrest and Sham mice (top), Sham+Metformin (Sham+Met) and Sham mice (middle), and Arrest+Metformin (Arrest+Met) to Arrest mice (bottom) based on microarray data collected from left ventricles one day after arrest (n = 6/group). Bar-graphs include significance values for pathway changes (−log(p-value)) and are colored according to z-score to predict directionality of changes. Differentially expressed genes were included in the analysis when p < 0.05 based on microarray data. Principal component analysis, volcano plots, and hierarchical clustering are included in .

Article Snippet: Microarray analysis was performed on cDNA through the Affymetrix microarray analysis service (ThermoFisher).

Techniques: Microarray