Journal: Melanoma Research

Article Title: Interfering with aggregated α-synuclein in advanced melanoma leads to a major upregulation of MHC class II proteins

doi: 10.1097/CMR.0000000000000982

Figure Lengend Snippet: Upregulation of MHC class II antigen in human melanoma xenografts treated systemically with anle138b, and expression of α-synuclein, MHC class II, and MHC class I proteins in melanoma TMA cores. (a) A tissue section from a nontreated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (b) A tissue section from an anle138b-treated WM983-B tumor xenograft probed with an antibody to human MHC class II (HLA-DPB1) protein (pseudocolored green). (c) Fluorescence intensity level of expression of proteins – α-synuclein (pseudocolored red), MHC II (pseudocolored yellow), MHC I (pseudocolored green) – in areas, encircled by a white line, in tissue sections from three of the Stage II and from one of the Stage IV melanoma TMA cores in which α-synuclein or vice versa, MHC II, or MHC I protein was expressed more strongly. TMA, tissue microarray.

Article Snippet: Four melanoma tissue microarray (TMA) slides ( https://www.biomax.us/tissue-arrays/Melanoma/ME811 ), each containing two tissue sections from the same 39 cases of melanoma, and tissue sections from three cases of normal skin tissue were heated at 60 °C, deparaffinized and rehydrated.

Techniques: Expressing, Fluorescence, Microarray

Journal: Scientific Reports

Article Title: Yes-Associated Protein (YAP) Promotes Tumorigenesis in Melanoma Cells Through Stimulation of Low-Density Lipoprotein Receptor-Related Protein 1 (LRP1)

doi: 10.1038/s41598-017-14764-4

Figure Lengend Snippet: The consistency of YAP and LRP1 in tissue microarray specimen. ( a , b ) TMA slides include forty skin melanoma tissues and eight skin normal tissues which locate on the bottom of the each TMA. Representative images of IHC from HCC TMA stained with anti-YAP or anti-LRP1 antibodies. Scale bar, 100 μM. ( c ) Representative images of IHC from skin melanoma HCC TMA stained with anti-YAP or anti-LRP1 antibodies. Scale bar, 100 μM. ( d ) The statistical figure of skin melanoma IHC images from HCC TMA stained with anti-YAP or anti-LRP1 antibodies. The TMA data were analyzed using the χ2 test.

Article Snippet: For IHC, melanoma tissue microarray analysis (TMA) slides were purchased from Biomax (Rockville, MD, USA).

Techniques: Microarray, Staining