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mcd-mimicking medium  (US Biological Life Sciences)

 
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    US Biological Life Sciences mcd-mimicking medium
    Mcd Mimicking Medium, supplied by US Biological Life Sciences, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mcd+medium/mcd+mimicking+medium/pm35568098-64-0-6
    Average 90 stars, based on 1 article reviews
    mcd-mimicking medium - by Bioz Stars, 2026-11
    90/100 stars

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    Related Articles

    other:

    Article Title: Indole supplementation ameliorates MCD-induced NASH in mice.
    Article Snippet: MCD-mimicking medium is a product from USBiological Life Sciences ( https://www.usbio.net/culture-media ).

    Article Title: Mice Lacking Adenosine 2A Receptor Reveal Increased Severity of MCD-induced NASH
    Article Snippet: Also, co-staining of F4/80 (rat anti-mouse, MCA497, Bio-Rad, Hercules, CA) and A 2A R (7F6-G5-A2, Cat# sc-32261, Santa Cruz Biotechnology, Inc., Dallas, TX, USA) in liver sections was evaluated by double immunofluorescent labeling according to the manufacturer’s instructions (Vector Laboratories, Inc. Burlingame, CA) as previously described ( Pei, et al. 2018 ).



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    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient <t>(MCD)</t> medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).
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    a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient (MCD) medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).

    Journal: Communications Biology

    Article Title: The spike of SARS-CoV-2 promotes metabolic rewiring in hepatocytes

    doi: 10.1038/s42003-022-03789-9

    Figure Lengend Snippet: a Western blot showing the modulation of ACE2 and NRP1 protein expression when incubated with methionine-choline deficient (MCD) medium for 6 and 24 h (along with their respective controls. Quantification of ACE2 or NRP1 protein expression by densitometry is also shown ( n = 3, unpaired t -test). b , c Western blot showing the expression of ACE2 and NRP1 protein expression when treated with 1 mM metformin for 6 or 24 h ( n = 3, unpaired t -test) ( b ) or with MCD or MCD plus 1 mM metformin for 10 h ( n = 3, one-way ANOVA) ( c ) as well as their respective controls. Relative quantification of ACE2 or NRP1 protein expression (related to β-actin) is also shown. d Relative infection with pseudotyped viral particles or controls of WT or hACE2 mouse hepatocytes (left) or upcyte second-generation human hepatocytes (right) upon treatment with MCD or MCD and metformin, measured by flow cytometry (one-way ANOVA). e Extracellular levels of ANG(1–7) secreted by human primary hepatocytes after infection with pseudotyped viral particles upon treatment with MCD or MCD and metformin, measured by ELISA ( n = 5, one-way ANOVA). Error bars represent SEM and asterisks represent p values (*<0.05, **<0.01, and ***<0.001).

    Article Snippet: Steatotic conditions were induced in primary hepatocytes as follows: incubation overnight on 0%-FBS MEM medium containing PSG followed by incubation in methionine-choline deficient (MCD) medium (Gibco Cat#: ME120128L1) supplemented with PSG.

    Techniques: Western Blot, Expressing, Incubation, Infection, Flow Cytometry, Enzyme-linked Immunosorbent Assay