mascot 2.0 search engine (Matrix Science)

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Matrix Science mascot 2.0 search engine
Mascot 2.0 Search Engine, supplied by Matrix Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mascot+algorithm/mascot+search+algorithm/pmc04541046-255-10-14
Average 90 stars, based on 1 article reviews

mascot 2.0 search engine - by Bioz Stars, 2026-11

90/100 stars

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Article Title: Diminished airway host innate response in people with Cystic Fibrosis who experience frequent exacerbations
Article Snippet: Database searching was carried out by the Mascot search engine (Matrix Science version 2.6.0), where samples were searched against a database containing all reviewed human sequences in the UniProt UniHumanReviewed database (updated 12/08/2020; 20,356 sequences; 11,357,197 residues).

Article Title: Supporting Information for Invasive mussels fashion silk-like byssus via mechanical processing of massive horizontally acquired coiled coils
Article Snippet: The raw data was converted into *.mgf format (Mascot generic format) for searching using the Mascot 2.6.2 search engine (Matrix Science).

Article Title: SFX-01 is therapeutic against myeloproliferative disorders caused by activating mutations in Shp2.
Article Snippet: The raw data files were processed and searched using the Mascot search algorithm (v2.6.0; www.matrixscience.com) and the Sequest search algorithm against the bespoke Shp2 database.

Article Title: EGFR phosphorylates and associates with EFNB1 to regulate cell adhesion to fibronectin.
Article Snippet: The Mascot search engine (Matrix Science, London, UK; version 2.8.0) was used to analyze the MGF sample files using the Uniprot Homo sapiens reference proteome (September 2022, 79759 entries) complemented with common contaminants from the Jo urn al Pr e-p roo f Global Proteome Machine (GPM, September 2020) and the YFP and miniT-Flag sequences assuming tryptic digestion.

Tandem Mass Spectroscopy:

Article Title: Ribosomopathy-like Properties of Murine and Human Cancers
Article Snippet: .. MS/MS spectra were searched using MASCOT search engine (Version 2.4.0, Matrix Science Ltd, London, UK) against the UniProt mouse proteome database. ..

Article Title: The Cullin3–Ring E3 ubiquitin ligase complex and USP14 regulate spastin-mediated microtubule severing and promotion of neurite outgrowth
Article Snippet: After chromatographic separation, the samples were analyzed using a Q Exactive mass spectrometer (Thermo Fisher Scientific) for 60 minutes. .. MS/MS spectra were searched against the UniProtKB database (https://www.uniprot.org/) employing the MASCOT search engine (Matrix Science; version 2.4, https://www.matrixscience.com/) to determine protein identities. .. 293T cells were co-transfected with the specified plasmids and a plasmid encoding FLAG-tagged ubiquitin for 48 hours, then treated with the proteasome inhibitor MG132 (20 μM, MedChemExpress, Cat# HY-13259, Monmouth Junction, NJ, USA) for 12 hours to inhibit proteasome activity and prevent degradation of ubiquitinated proteins.

Mass Spectrometry:

Article Title: A Novel Trypsin Inhibitor With Immunomodulatory Activity From the Leaves of Cissus verticillata subsp. verticillata (L.) Nicolson & C.E. Jarvis.
Article Snippet: In this study, we purified and characterized a trypsin inhibitor (CvLTI) from the leaves of Cissus verticillata subsp. verticillata.. CvLTI was isolated using a trypsin-agarose column and evaluated for stability towards heating (30C– 100C) and incubation at different pH values (2.0–10.0).. Furthermore, CvLTI was investigated for antibacterial activity against phytopathogens, hemolytic activity aswell as cytotoxicity and immunomodulatory effects on peripheral bloodmononuclear cells (PBMCs).

Software:

Article Title: Supporting Information
Article Snippet: For 60/90 minutes, LC-MS/MS analysis was carried out using a Q Exactive mass spectrometer (Thermo Scientific) linked to Easy nLC (Thermo Fisher Scientific). .. For identification and quantification analysis, the MASCOT engine (Matrix Science, London, UK; version 2.2) included in Proteome Discoverer 1.4 software was used to search the MS raw data for each sample. ..

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