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lunascript rt supermix kit  (New England Biolabs)


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    New England Biolabs lunascript rt supermix kit
    Lunascript Rt Supermix Kit, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 8895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lunascript+rt+master+mix+kit/LunaScript+RT+SuperMix+Kit/custom%40e3010%4036854624
    Average 99 stars, based on 8895 article reviews
    lunascript rt supermix kit - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    DNA Methylation Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transferring:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Countercurrent Chromatography:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transmission Electron Microscopy:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    In Vitro:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    In Vivo:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Flow Cytometry:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Blocking Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Standard Deviation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Negative Control:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transduction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Magnetic Beads:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Recombination Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Electron Microscopy:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Control Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Produced:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    High Performance Liquid Chromatography:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Cleavage Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Concentration Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Fluorescence:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    dsDNA Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Random Hexamer Labeling:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Digital PCR:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Plaque Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Injection:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    CRISPR:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    RNA Expression:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Hybridization:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Cytometry:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    MANN-WHITNEY:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Immunoprecipitation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Selection:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Derivative Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Polymerase Chain Reaction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Quantitative RT-PCR:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Sterility:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Sonication:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Real-time Polymerase Chain Reaction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    DNA Sequencing:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Marker:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Methylation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Mobility Shift:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Labeling:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Lamp Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Incubation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Activation Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Lateral Flow Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Modification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Multiplex Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Inhibition:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    In Silico:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Purification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Suspension:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Cell Culture:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Activity Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Quantitation Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Imaging:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Software:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    TBARS Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Enzyme-linked Immunosorbent Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Filtration:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Picogreen Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Serial Dilution:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    ChIP-sequencing:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Extraction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    RNA Extraction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Construct:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Bicinchoninic Acid Protein Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    HRM Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    TaqMan Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Gene Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Western Blot:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    E. coli Genomic Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    DNA Extraction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Cotransfection:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Northern Blot:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Chromatography:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Synthesized:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transfection:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Virus:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Agarose Gel Electrophoresis:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    FACS:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Ligation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Reporter Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Protein Extraction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Bacteria:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Saline:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Ethanol Precipitation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Spectrophotometry:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Membrane:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Sampling:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Hi-C:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Infection:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Over Expression:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    RNA Sequencing Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Diagnostic Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    In Situ:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Positive Control:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Cell Counting:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transformation Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Viability Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Protein Purification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Protease Inhibitor:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transmission Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Isolation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Staining:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Nucleic Acid Purification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Plasmid Preparation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Immunofluorescence:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Immunostaining:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Co-Culture Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Centrifugation:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Next-Generation Sequencing:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    SYBR Green Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Knock-Out:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Mass Spectrometry:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Expressing:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Protein Concentration:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    High Content Screening:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Transgenic Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Variant Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Virus Quantification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Recombinant:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Luciferase:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Mutagenesis:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Sequencing:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Titration:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Immunocytochemistry:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    SDS Page:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Clone Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Reporter Gene Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    DNA Purification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Generated:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Amplification:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Binding Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Nucleic Acid Electrophoresis:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Microscopy:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Lysis:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Functional Assay:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Comparison:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse

    Knock-In:

    Article Title: Doxorubicin‑induced cardiomyopathy is mitigated by empagliflozin via the modulation of endoplasmic reticulum stress pathways
    Article Snippet: d and reverse primers to a final volume of 10 µl. RT-PCR amplification was performed using a QuantStudio 3 Real Time PCR System (RRID:SCR_020238; Thermo Fisher Scientific, Inc.) with Luna Universal Master Mix (cat. no. M3003; New England Biolabs, Inc.). The program was set up to have initial denaturation at 95°C for 60 sec, followed by 40 cycles of denaturation at 95°C for 15 sec, and extension at 60°C for 30 sec. A continuous melt curve was subse



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