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Arraystar inc hybridization solution
Hybridization Solution, supplied by Arraystar inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lncrna+microarray+scanner/hybridization+solution/pm29794463-83-5-25
Average 90 stars, based on 1 article reviews
hybridization solution - by Bioz Stars, 2026-10
90/100 stars

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Hybridization:

Article Title: Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Article Snippet: .. Additionally, 50 μl of hybridization solution was injected into the spacer glass slide, assembled on the Arraystar Human circRNA V2 (8 × 15 K) chip (Arraystar, USA), and incubated in an Agilent hybridization oven at 65 °C for 17 h. After washing the slides, scan the array with G2505C gene scanner (Agilent, USA). .. The sequencing process of circRNA chip was carried out according to the protocol of KangChen Bio-tech, Shanghai, China.

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 °C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.
Article Snippet: Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer’s disease.. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer’s disease compared to healthy controls using circRNA microarrays.. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer’s disease patients.

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis.
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 ◦C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 ◦C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Injection:

Article Title: Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Article Snippet: .. Additionally, 50 μl of hybridization solution was injected into the spacer glass slide, assembled on the Arraystar Human circRNA V2 (8 × 15 K) chip (Arraystar, USA), and incubated in an Agilent hybridization oven at 65 °C for 17 h. After washing the slides, scan the array with G2505C gene scanner (Agilent, USA). .. The sequencing process of circRNA chip was carried out according to the protocol of KangChen Bio-tech, Shanghai, China.

Incubation:

Article Title: Expression profile of circRNA in peripheral blood mononuclear cells of patients with rheumatoid arthritis
Article Snippet: .. Additionally, 50 μl of hybridization solution was injected into the spacer glass slide, assembled on the Arraystar Human circRNA V2 (8 × 15 K) chip (Arraystar, USA), and incubated in an Agilent hybridization oven at 65 °C for 17 h. After washing the slides, scan the array with G2505C gene scanner (Agilent, USA). .. The sequencing process of circRNA chip was carried out according to the protocol of KangChen Bio-tech, Shanghai, China.

other:

Article Title: hsa_circRNA_100873 upregulation is associated with increased lymphatic metastasis of esophageal squamous cell carcinoma.
Article Snippet: Next, 25 μl 2x hybridization buffer (Arraystar Super RNA Tag kit; Arraystar) was added to dilute the labeled circRNA samples.

Article Title: hsa_circRNA_100873 upregulation is associated with increased lymphatic metastasis of esophageal squamous cell carcinoma
Article Snippet: Next, 25 μl 2× hybridization buffer (Arraystar Super RNA Tag kit; Arraystar) was added to dilute the labeled circRNA samples.

Labeling:

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 °C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 °C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Article Title: Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.
Article Snippet: Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer’s disease.. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer’s disease compared to healthy controls using circRNA microarrays.. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer’s disease patients.

Article Title: circRNA landscape in dorsal root ganglia from mice with collagen antibody-induced arthritis.
Article Snippet: The labeled circRNAs were purified by RNeasy Mini Kit (Qiagen) and 1 μg of each labeled circRNA was fragmented by adding 5 μl 10 × Blocking Agent and 1 μl of 25 × Fragmentation Buffer, then heated the mixture at 60 ◦C for 30 min. .. Finally, 25 μl 2 × Hybridization buffer was added to dilute the labeled cRNA and 50 μl of hybridization solution was dispensed into the gasket slide and assembled to the Arraystar Mouse circRNA Array V2 (8x15K, Arraystar Inc, 15 236 circRNAs). .. The slides were incubated for 17 h at 65 ◦C in an Agilent Hybridization Oven and after washing, the arrays were scanned by the Agilent Scanner G2505C (Agilent Technologies, Santa Clara, CA, USA).

Blocking Assay:

Article Title: Profiling of differentially expressed circular RNAs in peripheral blood mononuclear cells from Alzheimer's disease patients.
Article Snippet: Expression of circular RNA (circRNA), a class of noncoding RNAs that regulates gene expression, is altered in Alzheimer’s disease.. This study profiled differentially expressed circRNAs in peripheral blood mononuclear cells (PBMCs) from five patients with Alzheimer’s disease compared to healthy controls using circRNA microarrays.. We identified a total of 4060 differentially expressed circRNAs (1990 upregulated and 2070 downregulated) in Alzheimer’s disease patients.



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Differential <t>lncRNA</t> expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA <t>microarray</t> demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.
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Differential lncRNA expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA microarray demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.

Journal: Oncology Letters

Article Title: lncRNAs are novel biomarkers for differentiating between cisplatin-resistant and cisplatin-sensitive ovarian cancer

doi: 10.3892/ol.2018.8433

Figure Lengend Snippet: Differential lncRNA expression profiles between tissues of cisplatin-resistant and cisplatin-sensitive ovarian cancer. The lncRNA microarray demonstrated the differences between lncRNA expression in cisplatin-resistant and cisplatin-sensitive ovarian cancer through (A) hot-spot and (B) cluster map analysis. (C) Based on the association of the nearby coding genes, the differentially expressed lncRNAs were classified into certain types, including 312 natural antisense, 216 intronic antisense, 114 intron sense-overlapping, 673 intergenic, 201 exon sense-overlapping and 72 bidirectional lncRNA. lncRNA, long non-coding RNA.

Article Snippet: Following washing, the arrays were scanned using the Agilent lncRNA Microarray Scanner (Agilent Technologies, Inc.), and the Agilent Feature Extraction software version 11.0.1.1 (Agilent Technologies, Inc.) was used for microarray probe signal data collection.

Techniques: Expressing, Microarray