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inverted light microscope  (Nikon)


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    Structured Review

    Nikon inverted light microscope
    Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light <t>microscope</t> (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. * P<0.05 vs. SW1990. # P<0.05 vs. GEM + SW1990/GZ. ## P<0.01 vs. GEM + SW1990/GZ. Emo, emodin; GEM, gemcitabine; SW1990/GZ, GEM-resistant SW1990 cells; ALDH1, Aldehyde dehydrogenase 1; Snail, Snail family transcriptional repressor 1 gene.
    Inverted Light Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 14705 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/light+microscope+nikon-eclipse-e100/Inverted+Microscopes/pmc09748633-82-6-11
    Average 99 stars, based on 14705 article reviews
    inverted light microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    1) Product Images from "Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition"

    Article Title: Emodin reverses resistance to gemcitabine in pancreatic cancer by suppressing stemness through regulation of the epithelial‑mesenchymal transition

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2022.11706

    Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light microscope (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. * P<0.05 vs. SW1990. # P<0.05 vs. GEM + SW1990/GZ. ## P<0.01 vs. GEM + SW1990/GZ. Emo, emodin; GEM, gemcitabine; SW1990/GZ, GEM-resistant SW1990 cells; ALDH1, Aldehyde dehydrogenase 1; Snail, Snail family transcriptional repressor 1 gene.
    Figure Legend Snippet: Emo enhances inhibitory effects of GEM on self-renewal of SW1990/GZ cells. (A) Morphology of cells observed under an inverted light microscope (scale bar, 100 µm). (B) The ultrastructure of cells observed under a transmission electron microscope (x10,000 magnification). (C) Cell sphere-forming rate evaluated using the sphere formation assay (x100 magnification). (D) Number of spheres measured using the colony-formation assay (x200 magnification). (E) Expression levels of CD44, ALDH1, Nanog and Snail determined using western blotting assays. * P<0.05 vs. SW1990. # P<0.05 vs. GEM + SW1990/GZ. ## P<0.01 vs. GEM + SW1990/GZ. Emo, emodin; GEM, gemcitabine; SW1990/GZ, GEM-resistant SW1990 cells; ALDH1, Aldehyde dehydrogenase 1; Snail, Snail family transcriptional repressor 1 gene.

    Techniques Used: Light Microscopy, Transmission Assay, Microscopy, Tube Formation Assay, Colony Assay, Expressing, Western Blot

    Related Articles

    Fluorescence:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. Fluorescence images were captured using a Nikon inverted fluorescence microscope, and the MitoSOX Red fluorescence intensity was quantitatively analyzed using NIS-Elements AR software. ..

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.

    Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula
    Article Snippet: .. An inverted fluorescence microscope (Nikon, TiS, Japan) was applied to capture images. ..

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: The next day, after three PBS washes, fluorescently labeled secondary antibodies (Alexa Fluor 488 or 594, 1:500; Abcam) were added and incubated in the dark at room temperature for 1 h. Unbound secondary antibodies were washed away, and cell nuclei were stained with DAPI for 5 min. .. Finally, the cells were washed three times with PBS, mounted with anti-fluorescence quenching medium, and fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure data accuracy and reproducibility.

    Microscopy:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. Fluorescence images were captured using a Nikon inverted fluorescence microscope, and the MitoSOX Red fluorescence intensity was quantitatively analyzed using NIS-Elements AR software. ..

    Article Title: Structural Color Inkjet Printing With Mie-Resonant Silicon Nanoparticles.
    Article Snippet: Elemental mapping was performed using scanning transmission electron microscopy (STEM; JEM-2100F, JEOL) equipped with an energy-dispersive X-ray spectrometer (EDX; JED-2300, JEOL). .. Reflectance and transmittance spectra were measured via microspectroscopy using an inverted optical microscope (Ti-U, Nikon). ..

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.

    Article Title: Integrated fabrication of a shape-adaptable, antioxidative composite stent for effective closure and biological repair of enteroatmospheric fistula
    Article Snippet: .. An inverted fluorescence microscope (Nikon, TiS, Japan) was applied to capture images. ..

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: The next day, after three PBS washes, fluorescently labeled secondary antibodies (Alexa Fluor 488 or 594, 1:500; Abcam) were added and incubated in the dark at room temperature for 1 h. Unbound secondary antibodies were washed away, and cell nuclei were stained with DAPI for 5 min. .. Finally, the cells were washed three times with PBS, mounted with anti-fluorescence quenching medium, and fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure data accuracy and reproducibility.

    Software:

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. Fluorescence images were captured using a Nikon inverted fluorescence microscope, and the MitoSOX Red fluorescence intensity was quantitatively analyzed using NIS-Elements AR software. ..

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.

    Incubation:

    Article Title: MS4322 is a selective protein arginine methyltransferase 5 degrader with antitumor effects in cervical cancer cells
    Article Snippet: The cell monolayer was scraped with a 10 μl pipette tip to produce a wound, washed with PBS to remove detached cells and the cells were incubated in serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.). .. Cells were incubated at 37°C for 24 h. Photographs were taken at 0 and 24 h using a Nikon inverted light microscope (Nikon Instruments). .. Wound width analysis was performed with Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).

    Article Title: Synergistic mitochondrial homeostasis regulation and cholinergic circuits reconstruction via a one-step synthesized multifunctional hydrogel facilitates spinal cord injury repair
    Article Snippet: .. For ROS detection, 10 μM DCFH-DA or DHE working solution was added, followed by incubation at 37 °C in the dark for 30 min. For mtROS detection, PC12 cells was incubated with 5 μM MitoSox Red working solution under the same conditions in the dark for 15 min. Fluorescence images were acquired using a Nikon inverted fluorescence microscope (Nikon, Japan). .. All images were quantitatively analyzed using NIS-Elements AR software (Version 5.11, Nikon) to ensure the accuracy and reproducibility of experimental results.

    Light Microscopy:

    Article Title: MS4322 is a selective protein arginine methyltransferase 5 degrader with antitumor effects in cervical cancer cells
    Article Snippet: The cell monolayer was scraped with a 10 μl pipette tip to produce a wound, washed with PBS to remove detached cells and the cells were incubated in serum-free DMEM (Gibco; Thermo Fisher Scientific, Inc.). .. Cells were incubated at 37°C for 24 h. Photographs were taken at 0 and 24 h using a Nikon inverted light microscope (Nikon Instruments). .. Wound width analysis was performed with Image-Pro Plus 6.0 software (Media Cybernetics, Inc.).

    Staining:

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury
    Article Snippet: Sections were stained with a H&E Staining Kit (Beyotime, C0105S) according to the manufacturer's instructions. .. Stained sections were imaged on a Nikon inverted microscope and analyzed using ImageJ. ..

    Inverted Microscopy:

    Article Title: Histone H3K9 lactylation activates the TXNIP/NLRP3 pathway to drive macrophage inflammation after spinal cord injury
    Article Snippet: Sections were stained with a H&E Staining Kit (Beyotime, C0105S) according to the manufacturer's instructions. .. Stained sections were imaged on a Nikon inverted microscope and analyzed using ImageJ. ..

    Imaging:

    Article Title: Distribution and functional significance of rodent cerebellar glycogen
    Article Snippet: .. Epifluorescence images of coronal or sagittal sections were acquired using an inverted fluorescence microscope (Nikon ECLIPSE Ni-E, Mono-Camera Nikon DS-Fi3) controlled by proprietary software (NIS-Elements Imaging AR 4.60.00). .. Confocal images were acquired with a Nikon Eclipse Ti2 microscope (NIS-Elements AR 4.50.00) with a Plan Apo x60/1.40 numerical aperture (NA) oil-immersion objective at a Z step of 1 μm.



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