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ActivX Inc java applets
Java Applets, supplied by ActivX Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/java+applet/us07539818-80-21-21
Average 90 stars, based on 1 article reviews
java applets - by Bioz Stars, 2026-09
90/100 stars

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Article Title: System and methods for providing user analytics and performance feedback for web applications
Article Snippet: .. A server 16 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft IP telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a HTTP client; a FTP client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Virtual delivery appliance and system with remote authentication and related methods
Article Snippet: .. A server 16 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft IP telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; an HTTP client; a FTP client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Contextual turning of dialog from any app into actionable workspace notification
Article Snippet: .. A server 606 can execute, operate, or otherwise provide an application that can be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft Internet Protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a HyperText Transfer Protocol client; a File Transfer Protocol client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Email tracking
Article Snippet: .. A server 106 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft internet protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a hypertext transfer protocol (HTTP) client; a file transfer protocol (FTP) client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Secure file modification with supervision
Article Snippet: .. A server 204 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft IP telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a HTTP client; a FTP client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Application delivery controller performance capacity advisor
Article Snippet: .. A server 306 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft internet protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a hypertext transfer protocol (HTTP) client; a file transfer protocol (FTP) client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Proximity and context based stream playback control
Article Snippet: .. A server 406 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft internet protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a hypertext transfer protocol (HTTP) client; a file transfer protocol (FTP) client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Methods and systems for training and execution of improved learning systems for identification of components in time-based data streams
Article Snippet: .. A client 602 may execute, operate or otherwise provide an application, which can be any type and/or form of software, program, or executable instructions, including, without limitation, any type and/or form of web browser, web-based client, client-server application, an ActiveX control, a JAVA applet, a webserver, a database, an HPC (high performance computing) application, a data processing application, or any other type and/or form of executable instructions capable of executing on client 602. ..

Control:

Article Title: System and methods for providing user analytics and performance feedback for web applications
Article Snippet: .. A server 16 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft IP telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a HTTP client; a FTP client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Virtual delivery appliance and system with remote authentication and related methods
Article Snippet: .. A server 16 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft IP telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; an HTTP client; a FTP client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Contextual turning of dialog from any app into actionable workspace notification
Article Snippet: .. A server 606 can execute, operate, or otherwise provide an application that can be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft Internet Protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a HyperText Transfer Protocol client; a File Transfer Protocol client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Email tracking
Article Snippet: .. A server 106 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft internet protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a hypertext transfer protocol (HTTP) client; a file transfer protocol (FTP) client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Secure file modification with supervision
Article Snippet: .. A server 204 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft IP telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a HTTP client; a FTP client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Application delivery controller performance capacity advisor
Article Snippet: .. A server 306 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft internet protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a hypertext transfer protocol (HTTP) client; a file transfer protocol (FTP) client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Proximity and context based stream playback control
Article Snippet: .. A server 406 may execute, operate or otherwise provide an application that may be any one of the following: software; a program; executable instructions; a virtual machine; a hypervisor; a web browser; a web-based client; a client-server application; a thin-client computing client; an ActiveX control; a Java applet; software related to voice over internet protocol (VoIP) communications like a soft internet protocol telephone; an application for streaming video and/or audio; an application for facilitating real-time-data communications; a hypertext transfer protocol (HTTP) client; a file transfer protocol (FTP) client; an Oscar client; a Telnet client; or any other set of executable instructions. ..

Article Title: Methods and systems for training and execution of improved learning systems for identification of components in time-based data streams
Article Snippet: .. A client 602 may execute, operate or otherwise provide an application, which can be any type and/or form of software, program, or executable instructions, including, without limitation, any type and/or form of web browser, web-based client, client-server application, an ActiveX control, a JAVA applet, a webserver, a database, an HPC (high performance computing) application, a data processing application, or any other type and/or form of executable instructions capable of executing on client 602. ..



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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
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Statistik Georg Ferber statistik indonesia 2024
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Statistik Indonesia 2024, supplied by Statistik Georg Ferber, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/java+or+activex+software+components/2023+indonesia+statistik/10__64118_slash_aj__v3i1__71-129-0-0
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Statistik Georg Ferber cerita data statistik untuk indonesia
Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus <t>(A/Indonesia/5/2005),</t> clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.
Cerita Data Statistik Untuk Indonesia, supplied by Statistik Georg Ferber, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus (A/Indonesia/5/2005), clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.

Journal: Cell Reports Medicine

Article Title: Preclinical evaluation of an mRNA vaccine developed from the first human isolate of bovine H5N1

doi: 10.1016/j.xcrm.2026.102702

Figure Lengend Snippet: Comparison of vaccine efficacy between H5 Texas Mut vaccine and H5 Ast20 Mut vaccine in mice (A) Schematic of cleavage-defective mutant (Mut) HA mRNA vaccines. (B) Western blotting analysis of HA protein expression in transfected BHK-21 cells. Cells were transfected with varying doses (50, 100, 200, and 500 ng/μL) of Texas Mut or Ast20 Mut mRNA. (C) Mice were vaccinated (i.m.) with 1 or 5 μg Texas/Ast20 mRNA-LNP (SM102) encoding Mut HA or placebo using in a prime-boost schedule with an interval of 4 weeks. Blood samples ( n = 8) were collected from mice 2 weeks after prime and boost. Binding IgG of serum, reactive to the Texas HA protein, was measured by ELISA. Titers of placebo group were below detection limit. (D) 50% neutralization titers of pseudotyped virus (A/Texas/37/2024) in serum collected 2 weeks post-boost (week 6). Titers of placebo group were below detection limit. (E and F) Binding IgG of 1 μg Texas vaccine group (E) and Ast20 vaccine group (F) was measured by ELISA, reactive to the HA of clade 1 virus (A/Viet Nam/1203/2004), clade 2.1.3.2 virus (A/Indonesia/5/2005), clade 2.2 virus (A/bar-headed goose/Qinghai/5/2005), clade 2.3.2.1a virus (A/Hubei/1/2010), clade 2.3.4 virus (A/Anhui/1/2005), clade 2.3.4.4b virus (A/Astrakhan/3212/2020), and clade 2.3.4.4b virus (A/Texas/37/2024). (G) Radar chart of broad-binding IgG levels elicited by 1 μg Texas vaccine and Ast20 vaccine. (H) Vaccination schedule. Mice were vaccinated (i.m.) with 1 or 5 μg mRNA-LNP (SM102) encoding Texas HA-Mut, Ast20 HA-Mut, or placebo. Four weeks post-vaccination (week 4), mice were challenged with 50 × LD 50 of A/ostrich/China/HB/2024. Virus load of organs in vaccinated mice 1, 3, 5 days post-infection determined using plaque assays on MDCK cells. (I–L) Virus load of lung (I), heart (J), kidney (K), and brain (L). The dashed horizontal line indicates the assay limit of detection. Data are presented as the mean ± SD; p values are analyzed with t test (∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001) (C–L). ∗/∗∗∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (E and F) indicates significant differences between other HA proteins and Texas (E) or Ast20 (F) protein. ∗ (I–L) indicates significant differences between placebo and vaccination groups in the same day.

Article Snippet: HA recombinant protein of A/Indonesia/5/2005 , Synthesized from Novoprotein , N/A.

Techniques: Comparison, Mutagenesis, Vaccines, Western Blot, Expressing, Transfection, Binding Assay, Enzyme-linked Immunosorbent Assay, Neutralization, Virus, Infection