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TA Instruments itc analyze software
Itc Analyze Software, supplied by TA Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/itc+origin+analysis+software/itc+data+analysis+software/pmc07584215-189-5-5
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Related Articles

Titration:

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor
Article Snippet: For the (GlcNAc) 6 titrations, the cell contained either 0.1 mM Pichia -produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc) 6 (Megazyme). .. Data were corrected with a chito-oligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an independent binding model using TA Instruments ITC analysis software. .. Cloning and Protein Expression Pseudocercospora fuligena Avr4 (Pf-Avr4), residues 24 to 128, was cloned into the pPic9 vector (Invitrogen) between the Sma I and Not I restriction sites, resulting in an N-terminal 6xHistidine-FLAG tag, and was integrated into the genomic DNA of Pichia pastoris strain GS115.

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor.
Article Snippet: For the (GlcNAc)6 titrations, the cell contained either 0.1 mM Pichia-produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc)6 (Megazyme). .. Data were corrected with a chitooligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an D ow nloaded from https://academ ic.oup.com /plcell/article/28/8/1945/6100912 by Indian Institute of Technology Jodhpur user on 18 January 2024 independent binding model using TA Instruments ITC analysis software. ..

Article Title: The role of metal ion binding in the antioxidant mechanisms of reduced and oxidized glutathione in metal-mediated oxidative DNA damage
Article Snippet: .. D ow nl oa de d by U ni ve rs ity o f R ea di ng o n 1/ 3/ 20 20 2 :3 0: 53 A M . Metallomics This journal is©The Royal Society of Chemistry 2019 Cu(II)/GSSG ITC studies ITC studies of the Cu(II)/GSSG system were carried out using a TA Instruments Nano ITC Low Volume Isothermal Titration Calorimeter with a gold cell of active volume of 175 mL and TA Instruments ITC Runs acquisition software. .. Data was analyzed using TA Instruments NanoAnalyzes software.

Binding Assay:

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor
Article Snippet: For the (GlcNAc) 6 titrations, the cell contained either 0.1 mM Pichia -produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc) 6 (Megazyme). .. Data were corrected with a chito-oligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an independent binding model using TA Instruments ITC analysis software. .. Cloning and Protein Expression Pseudocercospora fuligena Avr4 (Pf-Avr4), residues 24 to 128, was cloned into the pPic9 vector (Invitrogen) between the Sma I and Not I restriction sites, resulting in an N-terminal 6xHistidine-FLAG tag, and was integrated into the genomic DNA of Pichia pastoris strain GS115.

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor.
Article Snippet: For the (GlcNAc)6 titrations, the cell contained either 0.1 mM Pichia-produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc)6 (Megazyme). .. Data were corrected with a chitooligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an D ow nloaded from https://academ ic.oup.com /plcell/article/28/8/1945/6100912 by Indian Institute of Technology Jodhpur user on 18 January 2024 independent binding model using TA Instruments ITC analysis software. ..

Software:

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor
Article Snippet: For the (GlcNAc) 6 titrations, the cell contained either 0.1 mM Pichia -produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc) 6 (Megazyme). .. Data were corrected with a chito-oligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an independent binding model using TA Instruments ITC analysis software. .. Cloning and Protein Expression Pseudocercospora fuligena Avr4 (Pf-Avr4), residues 24 to 128, was cloned into the pPic9 vector (Invitrogen) between the Sma I and Not I restriction sites, resulting in an N-terminal 6xHistidine-FLAG tag, and was integrated into the genomic DNA of Pichia pastoris strain GS115.

Article Title: Recognition‐Mediated Hydrogel Swelling Controlled by Interaction with a Negative Thermoresponsive LCST Polymer
Article Snippet: .. The fit and the determination of thermodynamic parameters (Ka, n, H, S, G) were achieved by using the TA Instrument ITC analysis software (NanoAnalyze). ..

Article Title: Structural Analysis of an Avr4 Effector Ortholog Offers Insight into Chitin Binding and Recognition by the Cf-4 Receptor.
Article Snippet: For the (GlcNAc)6 titrations, the cell contained either 0.1 mM Pichia-produced Pf-Avr4 or Cf-Avr4 and the syringe contained 2 mM (GlcNAc)6 (Megazyme). .. Data were corrected with a chitooligosaccharide blank titration, which consisted of a titration of the respective chito-oligosaccharide into buffer at equal volumes and concentrations used for the experimental titrations, and was fit with an D ow nloaded from https://academ ic.oup.com /plcell/article/28/8/1945/6100912 by Indian Institute of Technology Jodhpur user on 18 January 2024 independent binding model using TA Instruments ITC analysis software. ..

Article Title: Molecular mechanism underlying high-affinity terephthalate binding and conformational change of TBP from Ideonella sakaiensis.
Article Snippet: Ideonella sakaiensis is the bacterium that can survive by degrading polyethylene terephthalate (PET) plastic, and terephthalic acid (TPA) binding protein (IsTBP) is an essential periplasmic protein for uptake of TPA into the cytosol for complete degradation of PET.. Here, we demonstrated that IsTBP has remarkably high specificity for TPA among 33 monophenolic compounds and two 1,6-dicarboxylic acids tested.. Structural comparisons with 6carboxylic acid binding protein (RpAdpC) and TBP from Comamonas sp. E6 (CsTphC) revealed the key structural features that contribute to high TPA specificity and affinity of IsTBP.

Article Title: The role of metal ion binding in the antioxidant mechanisms of reduced and oxidized glutathione in metal-mediated oxidative DNA damage
Article Snippet: .. D ow nl oa de d by U ni ve rs ity o f R ea di ng o n 1/ 3/ 20 20 2 :3 0: 53 A M . Metallomics This journal is©The Royal Society of Chemistry 2019 Cu(II)/GSSG ITC studies ITC studies of the Cu(II)/GSSG system were carried out using a TA Instruments Nano ITC Low Volume Isothermal Titration Calorimeter with a gold cell of active volume of 175 mL and TA Instruments ITC Runs acquisition software. .. Data was analyzed using TA Instruments NanoAnalyzes software.

other:

Article Title: Chromatographic assay to probe the binding energy and mechanisms of homologous proteins to surface-bound ligands.
Article Snippet: Probing the affinity of a ligand for homologous protein targets currently relies on laborious assays that need special equipment and high amounts of isolated, highly pure proteins.. Herein we present the use of pISep, an integrated buffer system and modeling package, as an analytical method to rapidly and accurately probe the binding strength and mechanisms of homologous proteins to surface-bound ligands.. To demonstrate our method, we utilized the four subclasses of human immunoglobulin G (IgG) as model homologous protein targets and the IgGbinding peptide HWRGWV as model ligand.

Article Title: Molecular Dynamics-Based Design and Biophysical Evaluation of Thermostable Single-Chain Fv Antibody Mutants Derived from Pharmaceutical Antibodies
Article Snippet: All of the ITC data were analyzed using MicroCal Origin software (TA Instruments) with a One Set Sites Fitting Model.

Article Title: “The structure of the Type III secretion system export gate with CdsO, an ATPase lever arm”
Article Snippet: Calculations were performed using the TA instrument ITC Analyze software.

Analysis:

Article Title: Recognition‐Mediated Hydrogel Swelling Controlled by Interaction with a Negative Thermoresponsive LCST Polymer
Article Snippet: .. The fit and the determination of thermodynamic parameters (Ka, n, H, S, G) were achieved by using the TA Instrument ITC analysis software (NanoAnalyze). ..



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<t>ITC</t> traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.
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H3Q5ser stabilizes Taf3-PHD/H3K4me2 and Taf3-PHD/H3K4me3 complexes. ( A ) Experimental (blue) and fitted (red) spectra of free, ∼50% bound, and fully bound TAF3-PHD resonances E862 and C896 for H3K4me3/H3K4me3Q5ser complex. Transfer peaks indicated with *. Experimental spectrum of E862 is also shown separately for clarity. ( B ) <t>ITC</t> analysis of GST-tagged Taf3-PHD binding to H3K4me3 in yellow and H3K4me3Q5ser in navy blue. Top panel displays the raw heat change per injection of H3 peptide. Bottom panel represents the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software.
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ITC traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.

Journal: bioRxiv

Article Title: Biochemical Functions of the Membrane-Binding Domain of CARMIL

doi: 10.64898/2026.01.05.697744

Figure Lengend Snippet: ITC traces of CP titrated with CBR126 wt (panel A) or CBR126 mut (panel B). Upper panels are raw traces of thermograms, with differential power for each injection of CBR. Lower panels are binding isotherms, with integrated enthalpy vs molar ratio. Fitted values for K D , with a stoichiometry of one, are listed.

Article Snippet: Binding constants were determined by fitting the change in enthalpy to a single site binding model using MicroCal ITC Origin analysis software.

Techniques: Injection, Binding Assay

H3Q5ser stabilizes Taf3-PHD/H3K4me2 and Taf3-PHD/H3K4me3 complexes. ( A ) Experimental (blue) and fitted (red) spectra of free, ∼50% bound, and fully bound TAF3-PHD resonances E862 and C896 for H3K4me3/H3K4me3Q5ser complex. Transfer peaks indicated with *. Experimental spectrum of E862 is also shown separately for clarity. ( B ) ITC analysis of GST-tagged Taf3-PHD binding to H3K4me3 in yellow and H3K4me3Q5ser in navy blue. Top panel displays the raw heat change per injection of H3 peptide. Bottom panel represents the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software.

Journal: Nucleic Acids Research

Article Title: Molecular determinants for recognition of serotonylated chromatin

doi: 10.1093/nar/gkaf612

Figure Lengend Snippet: H3Q5ser stabilizes Taf3-PHD/H3K4me2 and Taf3-PHD/H3K4me3 complexes. ( A ) Experimental (blue) and fitted (red) spectra of free, ∼50% bound, and fully bound TAF3-PHD resonances E862 and C896 for H3K4me3/H3K4me3Q5ser complex. Transfer peaks indicated with *. Experimental spectrum of E862 is also shown separately for clarity. ( B ) ITC analysis of GST-tagged Taf3-PHD binding to H3K4me3 in yellow and H3K4me3Q5ser in navy blue. Top panel displays the raw heat change per injection of H3 peptide. Bottom panel represents the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software.

Article Snippet: ITC data were subsequently analysed and fitted with the one-site binding model in the MicroCal ITC Origin Analysis software 7.0 (Table , Figs and , , and ).

Techniques: Binding Assay, Injection, Software

Taf3-PHD P881 and W894 form key interaction surface with the serotonin group. ( A ) ITC analysis of GST-tagged Taf3-PHD mutants, BPTF-PHD , PHF2-PHD, or PHF8-PHD with the peptides H3K4me3 (in yellow) and H3K4me3Q5ser (in blue). Top panels display the raw heat change per injection of H3 peptide. Bottom panels represent the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software. ( B ) Multiple sequence alignment (MSA) of human PHD fingers for which high-resolution structures in complex with H3K4me3 are available (ASH1L PDB: 8VLF; BPTF(2) PDB: 2FUU; DIDO1 PDB: 4L7X; ING1 PDB: 2QIC; ING4 PDB: 2PNX/2VNF; ING5 PDB: 3C6W; KMT2E PDB: 4L58; PHF2 PDB: 3KQI; PHF13 PDB: 3O7A; UHRF1 PDB: 3SOW). Consensus Cys4–His–Cys3 positions are highlighted in grey and indicated on top. Locations of the secondary structure elements of TAF3-PHD are indicated on top. S880 and A902 are highlighted in purple, while P881 and W894 are highlighted in yellow.

Journal: Nucleic Acids Research

Article Title: Molecular determinants for recognition of serotonylated chromatin

doi: 10.1093/nar/gkaf612

Figure Lengend Snippet: Taf3-PHD P881 and W894 form key interaction surface with the serotonin group. ( A ) ITC analysis of GST-tagged Taf3-PHD mutants, BPTF-PHD , PHF2-PHD, or PHF8-PHD with the peptides H3K4me3 (in yellow) and H3K4me3Q5ser (in blue). Top panels display the raw heat change per injection of H3 peptide. Bottom panels represent the binding isotherm. The solid line represents the best fit to the one-set-of-sites model using MicroCal-ITC Origin 7 software. ( B ) Multiple sequence alignment (MSA) of human PHD fingers for which high-resolution structures in complex with H3K4me3 are available (ASH1L PDB: 8VLF; BPTF(2) PDB: 2FUU; DIDO1 PDB: 4L7X; ING1 PDB: 2QIC; ING4 PDB: 2PNX/2VNF; ING5 PDB: 3C6W; KMT2E PDB: 4L58; PHF2 PDB: 3KQI; PHF13 PDB: 3O7A; UHRF1 PDB: 3SOW). Consensus Cys4–His–Cys3 positions are highlighted in grey and indicated on top. Locations of the secondary structure elements of TAF3-PHD are indicated on top. S880 and A902 are highlighted in purple, while P881 and W894 are highlighted in yellow.

Article Snippet: ITC data were subsequently analysed and fitted with the one-site binding model in the MicroCal ITC Origin Analysis software 7.0 (Table , Figs and , , and ).

Techniques: Injection, Binding Assay, Software, Sequencing