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fastpure cell tissue dna isolation kit  (Vazyme Biotech Co)


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    Vazyme Biotech Co fastpure cell tissue dna isolation kit
    Fastpure Cell Tissue Dna Isolation Kit, supplied by Vazyme Biotech Co, used in various techniques. Bioz Stars score: 97/100, based on 526 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolators/FastPure+Cell+Tissue+DNA+Isolation+Mini+Kit/pmc12965145-45-0-9
    Average 97 stars, based on 526 article reviews
    fastpure cell tissue dna isolation kit - by Bioz Stars, 2026-10
    97/100 stars

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    Related Articles

    H&E Stain:

    Article Title: AIM2 in tregs restrains anti-PD-1 efficacy by reinforcing immune suppression in MC38 colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 2× Taq Plus Master Mix II (Dye Plus) Vazyme Cat# P213-02 Hematoxylin and Eosin (H&E) staining reagents Solarbio Life Sciences Cat# H8070 RPMI1640 (medium) Gibco (Thermo Fisher Scientific) Cat# 11875093 Critical commercial assays FastPure Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 CellTraceTM Violet Cell Proliferation Kit Invitrogen (Thermo Fisher Scientific) Cat# C34557 Deposited data RNA-seq CNGB CNP0009444 Experimental models: Cell lines Cell line (Mus musculus): MC38 Cell Bank of the Chinese Academy of Sciences RRID: CVCL_B288 Cell line (Mus musculus): B16 Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: Foxp3YFP−Cre Jackson Laboratory RRID: IMSR_JAX:016959 Mouse: Aim2fl/fl Foxp3YFP−Cre Jiangsu Jicui Yaokang Biotechnology Co., Ltd. Strain NO. T009247 Oligonucleotides JS03123-Aim2-5wt-tF1 CTTGCCAATGTACTCAACAGTTCTC Primer ID: A2250 JS03123-Aim2-5wt-tR1 AGAAGGTAACAGAGGTGCCATCTAG Primer ID: A2251 JS03123-Aim2-3wt-tF1 ACAGAGTTCAGGATTTGTAGGATG Primer ID: A2252 JS03123-Aim2-3wt-tR1 ACCCAACAGTGACTTTGAATATGG Primer ID: A2253 14125 CCTAGCCCCTAGTTCCAACC Primer ID: 6206 14126 AAGGTTCCAGTGCTGTTGCT Primer ID: 6207 16250 AGGATGTGAGGGACTACCTCCTGTA Primer ID: 5627 16251 TCCTTCACTCTGATTCTGGCAATTT Primer ID: 5628 Software and algorithms ImageJ https://www.imagej.net/ RRID: SCR_003070 Flowjo https://www.flowjo.com RRID:SCR_008520 Adobe Illustrator https://www.adobe.com/products/ illustrator.html RRID:SCR_010279 GraphPad Prism https://www.graphpad.com/ RRID:SCR_002798 R | The R Project for Statistical Computing https://www.r-project.org RRID:SCR_001905 RStudio https://www.rstudio.com RRID:SCR_000432 16 iScience 29, 116082, October 16, 2026 ..

    Tissue:

    Article Title: AIM2 in tregs restrains anti-PD-1 efficacy by reinforcing immune suppression in MC38 colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 2× Taq Plus Master Mix II (Dye Plus) Vazyme Cat# P213-02 Hematoxylin and Eosin (H&E) staining reagents Solarbio Life Sciences Cat# H8070 RPMI1640 (medium) Gibco (Thermo Fisher Scientific) Cat# 11875093 Critical commercial assays FastPure Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 CellTraceTM Violet Cell Proliferation Kit Invitrogen (Thermo Fisher Scientific) Cat# C34557 Deposited data RNA-seq CNGB CNP0009444 Experimental models: Cell lines Cell line (Mus musculus): MC38 Cell Bank of the Chinese Academy of Sciences RRID: CVCL_B288 Cell line (Mus musculus): B16 Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: Foxp3YFP−Cre Jackson Laboratory RRID: IMSR_JAX:016959 Mouse: Aim2fl/fl Foxp3YFP−Cre Jiangsu Jicui Yaokang Biotechnology Co., Ltd. Strain NO. T009247 Oligonucleotides JS03123-Aim2-5wt-tF1 CTTGCCAATGTACTCAACAGTTCTC Primer ID: A2250 JS03123-Aim2-5wt-tR1 AGAAGGTAACAGAGGTGCCATCTAG Primer ID: A2251 JS03123-Aim2-3wt-tF1 ACAGAGTTCAGGATTTGTAGGATG Primer ID: A2252 JS03123-Aim2-3wt-tR1 ACCCAACAGTGACTTTGAATATGG Primer ID: A2253 14125 CCTAGCCCCTAGTTCCAACC Primer ID: 6206 14126 AAGGTTCCAGTGCTGTTGCT Primer ID: 6207 16250 AGGATGTGAGGGACTACCTCCTGTA Primer ID: 5627 16251 TCCTTCACTCTGATTCTGGCAATTT Primer ID: 5628 Software and algorithms ImageJ https://www.imagej.net/ RRID: SCR_003070 Flowjo https://www.flowjo.com RRID:SCR_008520 Adobe Illustrator https://www.adobe.com/products/ illustrator.html RRID:SCR_010279 GraphPad Prism https://www.graphpad.com/ RRID:SCR_002798 R | The R Project for Statistical Computing https://www.r-project.org RRID:SCR_001905 RStudio https://www.rstudio.com RRID:SCR_000432 16 iScience 29, 116082, October 16, 2026 ..

    Article Title: Microbial signaling coordinates group oviposition and predator defense in migratory locusts.
    Article Snippet: .. Equal amounts of gut tissue were collected from germ-depleted and untreated control females, and genomic DNA was extracted using the FastPure Cell/Tissue DNA Isolation Mini Kit (Vazyme, China) according to the manufacturer’s instructions. .. Quantitative PCR was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad) using Taq Pro Universal SYBR qPCR Master Mix (Vazyme, China).

    Article Title: A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri.
    Article Snippet: First-strand cDNA was synthesized with 1000 ng of total RNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper; Vazyme). .. Genomic DNA was isolated using the FastPure Cell Tissue DNA Isolation Mini Kit (Vazyme, China). .. RNA and DNA concentration and purity were evaluated using a NanoDrop spectrophotometer (Thermo Fisher Scientific, New York, NY, USA).

    DNA Extraction:

    Article Title: AIM2 in tregs restrains anti-PD-1 efficacy by reinforcing immune suppression in MC38 colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 2× Taq Plus Master Mix II (Dye Plus) Vazyme Cat# P213-02 Hematoxylin and Eosin (H&E) staining reagents Solarbio Life Sciences Cat# H8070 RPMI1640 (medium) Gibco (Thermo Fisher Scientific) Cat# 11875093 Critical commercial assays FastPure Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 CellTraceTM Violet Cell Proliferation Kit Invitrogen (Thermo Fisher Scientific) Cat# C34557 Deposited data RNA-seq CNGB CNP0009444 Experimental models: Cell lines Cell line (Mus musculus): MC38 Cell Bank of the Chinese Academy of Sciences RRID: CVCL_B288 Cell line (Mus musculus): B16 Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: Foxp3YFP−Cre Jackson Laboratory RRID: IMSR_JAX:016959 Mouse: Aim2fl/fl Foxp3YFP−Cre Jiangsu Jicui Yaokang Biotechnology Co., Ltd. Strain NO. T009247 Oligonucleotides JS03123-Aim2-5wt-tF1 CTTGCCAATGTACTCAACAGTTCTC Primer ID: A2250 JS03123-Aim2-5wt-tR1 AGAAGGTAACAGAGGTGCCATCTAG Primer ID: A2251 JS03123-Aim2-3wt-tF1 ACAGAGTTCAGGATTTGTAGGATG Primer ID: A2252 JS03123-Aim2-3wt-tR1 ACCCAACAGTGACTTTGAATATGG Primer ID: A2253 14125 CCTAGCCCCTAGTTCCAACC Primer ID: 6206 14126 AAGGTTCCAGTGCTGTTGCT Primer ID: 6207 16250 AGGATGTGAGGGACTACCTCCTGTA Primer ID: 5627 16251 TCCTTCACTCTGATTCTGGCAATTT Primer ID: 5628 Software and algorithms ImageJ https://www.imagej.net/ RRID: SCR_003070 Flowjo https://www.flowjo.com RRID:SCR_008520 Adobe Illustrator https://www.adobe.com/products/ illustrator.html RRID:SCR_010279 GraphPad Prism https://www.graphpad.com/ RRID:SCR_002798 R | The R Project for Statistical Computing https://www.r-project.org RRID:SCR_001905 RStudio https://www.rstudio.com RRID:SCR_000432 16 iScience 29, 116082, October 16, 2026 ..

    Article Title: Microbial signaling coordinates group oviposition and predator defense in migratory locusts.
    Article Snippet: .. Equal amounts of gut tissue were collected from germ-depleted and untreated control females, and genomic DNA was extracted using the FastPure Cell/Tissue DNA Isolation Mini Kit (Vazyme, China) according to the manufacturer’s instructions. .. Quantitative PCR was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad) using Taq Pro Universal SYBR qPCR Master Mix (Vazyme, China).

    Article Title: Mitochondrial transfer protects against cisplatin-induced hearing loss by fumarate-mediated activation of mitophagy.
    Article Snippet: Imaging was performed via a scanning electron microscope (Hitachi). .. Genomic DNA was extracted from cells via the FastPure Cell/Tissue DNA Isolation Mini Kit (Cat#: DC102-01, Vazyme) according to the manufacturer’s instructions. ..

    Article Title: E3 Ubiquitin Ligase RNF126 Contributes to Pathogenesis of Obesity by Induction Mitophagy Mediated by PINK1 /Parkin Pathway
    Article Snippet: Obesity and its associated metabolic disorders have become major global public health challenges.. Increasing evidence indicates that mitophagy plays a pivotal role in the pathogenesis of obesity and related metabolic diseases.. This study aimed to investigate the role and underlying mechanisms of E3 ubiquitin ligase RNF126 in obesity and nonalcoholic fatty liver disease (NAFLD).

    Article Title: Mechanistic insights into chromatin modulation by the "orphan" remodeler ATRX.
    Article Snippet: .. Genomic DNA was extracted using Cell/Tissue DNA Isolation Mini Kit (Vazyme, Cat# DC102) according to the manufacturer’s instructions. ..

    Article Title: Sequential TPRX1 and HERVL activation delineates a progression toward the totipotent-like state.
    Article Snippet: The 4CL medium was prepared by mixing equal parts of Neurobasal medium (Gibco, 21103049) and DMEM/F12 (Gibco, 11330057), and it was supplemented with 1×N2 (Gibco, 17502048), 1×B27 (Gibco, 17504044), 1×sodium pyruvate .. REAGENT or RESOURCE SOURCE IDENTIFIER Bowtie2 2.2.2 Langmead and Salzberg30 https://bowtie-bio.sourceforge.net/bowtie2 Picard 2.27.5 Broad Institute https://broadinstitute.github.io/picard deepTools 3.1.3 Ramı́rez et al.31 https://deeptools.readthedocs.io MACS2 2.2.7.1 Zhang et al.32 https://github.com/macs3-project/MACS BEDTools 2.29.0 Quinlan laboratory33 https://bedtools.readthedocs.io FactoMineR 2.14 CRNA https://cran.r-project.org/package=FactoMineR sva 3.46.0 Bioconductor https://bioconductor.org/packages/sva/ R 4.5.1 R Core Team https://www.r-project.org Other PGM1 medium Cellapy Cat# CA1007500 Neurobasal medium Gibco Cat# 21103049 DMEM/F12 Gibco Cat# 11330057 DNA Isolation Kit Vazyme Cat# DC102-01 Confocal dishes Ibidi Cat# 80826 & 80806 VAHTS Universal V8 RNA-seq Library Prep Kit Vazyme Cat# NR605 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme Cat# TD711 TruePrep Index Kit V2 Vazyme Cat# TD202 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat# TD904 16 Cell Reports 45, 118163, November 24, 2026 (Gibco, 11360070), 1× non-essential amino acids (Gibco, 11140050), 1× GlutaMAX (Gibco, 35050061), 1× penicillin-streptomycin (Gibco, 15140122), 10 nM DZNep (MedChemExpress, HY-12186), 5 nM TSA (MedChemExpress, HY-15144), 1 μM PD0325901 (MedChemExpress, HY-10254), 5 μM IWR-1 (MedChemExpress, HY-12238), 20 ng/mL human LIF (Peprotech, 300–05), 20 ng/mL ACTIVIN A (Peprotech, 120-14E), 50 μg/mL L-ascorbic acid (Sigma, 49752), and 0.4% (v/v) Geltrex (Invitrogen, A1413302) or Matrigel (Corning, 354230). .. The 4CL medium was refreshed daily, and the cells were passaged as single cells every 3 days, with a split ratio of 1:5 to 1:8, using Accutase (Gibco, 00–4555-56).

    Article Title: A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri.
    Article Snippet: First-strand cDNA was synthesized with 1000 ng of total RNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper; Vazyme). .. Genomic DNA was isolated using the FastPure Cell Tissue DNA Isolation Mini Kit (Vazyme, China). .. RNA and DNA concentration and purity were evaluated using a NanoDrop spectrophotometer (Thermo Fisher Scientific, New York, NY, USA).

    Article Title: D-allose reprogrammes the mitophagy-mtDNA-cGAS-STING-ADM2 axis to restore nucleus pulposus cell homeostasis in intervertebral disc degeneration.
    Article Snippet: .. DNA was then purified from this cytosolic fraction using the FastPure Cell/Tissue DNA Isolation Mini Kit (DC102-01, Vazyme, China). ..

    RNA Sequencing:

    Article Title: AIM2 in tregs restrains anti-PD-1 efficacy by reinforcing immune suppression in MC38 colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 2× Taq Plus Master Mix II (Dye Plus) Vazyme Cat# P213-02 Hematoxylin and Eosin (H&E) staining reagents Solarbio Life Sciences Cat# H8070 RPMI1640 (medium) Gibco (Thermo Fisher Scientific) Cat# 11875093 Critical commercial assays FastPure Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 CellTraceTM Violet Cell Proliferation Kit Invitrogen (Thermo Fisher Scientific) Cat# C34557 Deposited data RNA-seq CNGB CNP0009444 Experimental models: Cell lines Cell line (Mus musculus): MC38 Cell Bank of the Chinese Academy of Sciences RRID: CVCL_B288 Cell line (Mus musculus): B16 Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: Foxp3YFP−Cre Jackson Laboratory RRID: IMSR_JAX:016959 Mouse: Aim2fl/fl Foxp3YFP−Cre Jiangsu Jicui Yaokang Biotechnology Co., Ltd. Strain NO. T009247 Oligonucleotides JS03123-Aim2-5wt-tF1 CTTGCCAATGTACTCAACAGTTCTC Primer ID: A2250 JS03123-Aim2-5wt-tR1 AGAAGGTAACAGAGGTGCCATCTAG Primer ID: A2251 JS03123-Aim2-3wt-tF1 ACAGAGTTCAGGATTTGTAGGATG Primer ID: A2252 JS03123-Aim2-3wt-tR1 ACCCAACAGTGACTTTGAATATGG Primer ID: A2253 14125 CCTAGCCCCTAGTTCCAACC Primer ID: 6206 14126 AAGGTTCCAGTGCTGTTGCT Primer ID: 6207 16250 AGGATGTGAGGGACTACCTCCTGTA Primer ID: 5627 16251 TCCTTCACTCTGATTCTGGCAATTT Primer ID: 5628 Software and algorithms ImageJ https://www.imagej.net/ RRID: SCR_003070 Flowjo https://www.flowjo.com RRID:SCR_008520 Adobe Illustrator https://www.adobe.com/products/ illustrator.html RRID:SCR_010279 GraphPad Prism https://www.graphpad.com/ RRID:SCR_002798 R | The R Project for Statistical Computing https://www.r-project.org RRID:SCR_001905 RStudio https://www.rstudio.com RRID:SCR_000432 16 iScience 29, 116082, October 16, 2026 ..

    Article Title: Sequential TPRX1 and HERVL activation delineates a progression toward the totipotent-like state.
    Article Snippet: The 4CL medium was prepared by mixing equal parts of Neurobasal medium (Gibco, 21103049) and DMEM/F12 (Gibco, 11330057), and it was supplemented with 1×N2 (Gibco, 17502048), 1×B27 (Gibco, 17504044), 1×sodium pyruvate .. REAGENT or RESOURCE SOURCE IDENTIFIER Bowtie2 2.2.2 Langmead and Salzberg30 https://bowtie-bio.sourceforge.net/bowtie2 Picard 2.27.5 Broad Institute https://broadinstitute.github.io/picard deepTools 3.1.3 Ramı́rez et al.31 https://deeptools.readthedocs.io MACS2 2.2.7.1 Zhang et al.32 https://github.com/macs3-project/MACS BEDTools 2.29.0 Quinlan laboratory33 https://bedtools.readthedocs.io FactoMineR 2.14 CRNA https://cran.r-project.org/package=FactoMineR sva 3.46.0 Bioconductor https://bioconductor.org/packages/sva/ R 4.5.1 R Core Team https://www.r-project.org Other PGM1 medium Cellapy Cat# CA1007500 Neurobasal medium Gibco Cat# 21103049 DMEM/F12 Gibco Cat# 11330057 DNA Isolation Kit Vazyme Cat# DC102-01 Confocal dishes Ibidi Cat# 80826 & 80806 VAHTS Universal V8 RNA-seq Library Prep Kit Vazyme Cat# NR605 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme Cat# TD711 TruePrep Index Kit V2 Vazyme Cat# TD202 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat# TD904 16 Cell Reports 45, 118163, November 24, 2026 (Gibco, 11360070), 1× non-essential amino acids (Gibco, 11140050), 1× GlutaMAX (Gibco, 35050061), 1× penicillin-streptomycin (Gibco, 15140122), 10 nM DZNep (MedChemExpress, HY-12186), 5 nM TSA (MedChemExpress, HY-15144), 1 μM PD0325901 (MedChemExpress, HY-10254), 5 μM IWR-1 (MedChemExpress, HY-12238), 20 ng/mL human LIF (Peprotech, 300–05), 20 ng/mL ACTIVIN A (Peprotech, 120-14E), 50 μg/mL L-ascorbic acid (Sigma, 49752), and 0.4% (v/v) Geltrex (Invitrogen, A1413302) or Matrigel (Corning, 354230). .. The 4CL medium was refreshed daily, and the cells were passaged as single cells every 3 days, with a split ratio of 1:5 to 1:8, using Accutase (Gibco, 00–4555-56).

    Software:

    Article Title: AIM2 in tregs restrains anti-PD-1 efficacy by reinforcing immune suppression in MC38 colorectal cancer.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER 2× Taq Plus Master Mix II (Dye Plus) Vazyme Cat# P213-02 Hematoxylin and Eosin (H&E) staining reagents Solarbio Life Sciences Cat# H8070 RPMI1640 (medium) Gibco (Thermo Fisher Scientific) Cat# 11875093 Critical commercial assays FastPure Tissue DNA Isolation Mini Kit Vazyme Cat# DC102-01 CellTraceTM Violet Cell Proliferation Kit Invitrogen (Thermo Fisher Scientific) Cat# C34557 Deposited data RNA-seq CNGB CNP0009444 Experimental models: Cell lines Cell line (Mus musculus): MC38 Cell Bank of the Chinese Academy of Sciences RRID: CVCL_B288 Cell line (Mus musculus): B16 Cell Bank of the Chinese Academy of Sciences N/A Experimental models: Organisms/strains Mouse: Foxp3YFP−Cre Jackson Laboratory RRID: IMSR_JAX:016959 Mouse: Aim2fl/fl Foxp3YFP−Cre Jiangsu Jicui Yaokang Biotechnology Co., Ltd. Strain NO. T009247 Oligonucleotides JS03123-Aim2-5wt-tF1 CTTGCCAATGTACTCAACAGTTCTC Primer ID: A2250 JS03123-Aim2-5wt-tR1 AGAAGGTAACAGAGGTGCCATCTAG Primer ID: A2251 JS03123-Aim2-3wt-tF1 ACAGAGTTCAGGATTTGTAGGATG Primer ID: A2252 JS03123-Aim2-3wt-tR1 ACCCAACAGTGACTTTGAATATGG Primer ID: A2253 14125 CCTAGCCCCTAGTTCCAACC Primer ID: 6206 14126 AAGGTTCCAGTGCTGTTGCT Primer ID: 6207 16250 AGGATGTGAGGGACTACCTCCTGTA Primer ID: 5627 16251 TCCTTCACTCTGATTCTGGCAATTT Primer ID: 5628 Software and algorithms ImageJ https://www.imagej.net/ RRID: SCR_003070 Flowjo https://www.flowjo.com RRID:SCR_008520 Adobe Illustrator https://www.adobe.com/products/ illustrator.html RRID:SCR_010279 GraphPad Prism https://www.graphpad.com/ RRID:SCR_002798 R | The R Project for Statistical Computing https://www.r-project.org RRID:SCR_001905 RStudio https://www.rstudio.com RRID:SCR_000432 16 iScience 29, 116082, October 16, 2026 ..

    Control:

    Article Title: Microbial signaling coordinates group oviposition and predator defense in migratory locusts.
    Article Snippet: .. Equal amounts of gut tissue were collected from germ-depleted and untreated control females, and genomic DNA was extracted using the FastPure Cell/Tissue DNA Isolation Mini Kit (Vazyme, China) according to the manufacturer’s instructions. .. Quantitative PCR was performed on a CFX96 Real-Time PCR Detection System (Bio-Rad) using Taq Pro Universal SYBR qPCR Master Mix (Vazyme, China).

    Confocal:

    Article Title: Sequential TPRX1 and HERVL activation delineates a progression toward the totipotent-like state.
    Article Snippet: The 4CL medium was prepared by mixing equal parts of Neurobasal medium (Gibco, 21103049) and DMEM/F12 (Gibco, 11330057), and it was supplemented with 1×N2 (Gibco, 17502048), 1×B27 (Gibco, 17504044), 1×sodium pyruvate .. REAGENT or RESOURCE SOURCE IDENTIFIER Bowtie2 2.2.2 Langmead and Salzberg30 https://bowtie-bio.sourceforge.net/bowtie2 Picard 2.27.5 Broad Institute https://broadinstitute.github.io/picard deepTools 3.1.3 Ramı́rez et al.31 https://deeptools.readthedocs.io MACS2 2.2.7.1 Zhang et al.32 https://github.com/macs3-project/MACS BEDTools 2.29.0 Quinlan laboratory33 https://bedtools.readthedocs.io FactoMineR 2.14 CRNA https://cran.r-project.org/package=FactoMineR sva 3.46.0 Bioconductor https://bioconductor.org/packages/sva/ R 4.5.1 R Core Team https://www.r-project.org Other PGM1 medium Cellapy Cat# CA1007500 Neurobasal medium Gibco Cat# 21103049 DMEM/F12 Gibco Cat# 11330057 DNA Isolation Kit Vazyme Cat# DC102-01 Confocal dishes Ibidi Cat# 80826 & 80806 VAHTS Universal V8 RNA-seq Library Prep Kit Vazyme Cat# NR605 Hyperactive ATAC-Seq Library Prep Kit for Illumina Vazyme Cat# TD711 TruePrep Index Kit V2 Vazyme Cat# TD202 Hyperactive Universal CUT&Tag Assay Kit for Illumina Vazyme Cat# TD904 16 Cell Reports 45, 118163, November 24, 2026 (Gibco, 11360070), 1× non-essential amino acids (Gibco, 11140050), 1× GlutaMAX (Gibco, 35050061), 1× penicillin-streptomycin (Gibco, 15140122), 10 nM DZNep (MedChemExpress, HY-12186), 5 nM TSA (MedChemExpress, HY-15144), 1 μM PD0325901 (MedChemExpress, HY-10254), 5 μM IWR-1 (MedChemExpress, HY-12238), 20 ng/mL human LIF (Peprotech, 300–05), 20 ng/mL ACTIVIN A (Peprotech, 120-14E), 50 μg/mL L-ascorbic acid (Sigma, 49752), and 0.4% (v/v) Geltrex (Invitrogen, A1413302) or Matrigel (Corning, 354230). .. The 4CL medium was refreshed daily, and the cells were passaged as single cells every 3 days, with a split ratio of 1:5 to 1:8, using Accutase (Gibco, 00–4555-56).

    Isolation:

    Article Title: A nuclear receptor PcHR96h regulates ABC transporters involved in spirodiclofen resistance in Panonychus citri.
    Article Snippet: First-strand cDNA was synthesized with 1000 ng of total RNA using the HiScript III 1st Strand cDNA Synthesis Kit (+gDNA wiper; Vazyme). .. Genomic DNA was isolated using the FastPure Cell Tissue DNA Isolation Mini Kit (Vazyme, China). .. RNA and DNA concentration and purity were evaluated using a NanoDrop spectrophotometer (Thermo Fisher Scientific, New York, NY, USA).

    Purification:

    Article Title: D-allose reprogrammes the mitophagy-mtDNA-cGAS-STING-ADM2 axis to restore nucleus pulposus cell homeostasis in intervertebral disc degeneration.
    Article Snippet: .. DNA was then purified from this cytosolic fraction using the FastPure Cell/Tissue DNA Isolation Mini Kit (DC102-01, Vazyme, China). ..



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    Mouse Cd8 T Cell Isolation Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/isolators/CD8a%2B+T+Cell+Isolation+Kit%2C+mouse/pm42882451-142-28-35
    Average 97 stars, based on 1 article reviews
    mouse cd8 t cell isolation kit - by Bioz Stars, 2026-10
    97/100 stars
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