Control:Article Title: Comparison of two commercial quality control sera for adrenocorticotropin ( ACTH ) used in Elecsys ® immunoassay system
Article Snippet: .. However, after 2 hours of storage at 4°C, the decline in the measured ACTH IQC was 8.04%, 11.84%, and 10.11%, respectively (Figure B). table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Mean SEM CV% Bio‐Rad—level 1 20.21 20.08 20.08 20.12 0.08 0.37 15.60 15.49 15.52 15.54 0.06 0.37 14.32 14.53 14.74 14.53 0.21 1.45 14.63 14.59 14.29 14.50 0.19 1.28 15.06 14.79 14.77 14.87 0.16 1.09 Total 15.91 2.22 13.93 Bio‐Rad—level 2 67.51 66.46 66.67 66.88 0.56 0.83 49.32 49.20 49.30 49.27 0.06 0.13 45.70 45.78 45.38 45.62 0.21 0.46 45.41 45.03 44.99 45.14 0.23 0.51 48.22 48.06 47.53 47.94 0.36 0.75 Total 50.97 8.39 16.45 Bio‐Rad—level 3 103.70 103.90 102.89 103.50 0.53 0.52 79.07 78.22 78.56 78.62 0.43 0.54 71.46 71.26 70.29 71.00 0.63 0.88 71.06 71.47 70.62 71.05 0.43 0.60 74.14 73.08 73.80 73.67 0.54 0.73 Total 79.57 12.72 15.98 Roche—level 1 41.96 40.25 40.49 40.90 0.93 2.26 40.03 40.33 40.51 40.29 0.24 0.60 41.21 40.85 41.12 41.06 0.19 0.46 40.25 40.82 40.85 40.64 0.34 0.83 40.89 40.65 40.67 40.74 0.13 0.33 Total 40.73 0.48 1.17 Roche—level 2 864.9 878.5 869.3 870.90 6.94 0.80 861.7 862.9 862.5 862.37 0.61 0.07 857.0 864.2 854.3 858.50 5.12 0.60 856.5 850.8 856.0 854.43 3.16 0.37 841.2 846.6 852.2 846.67 5.50 0.65 Total 858.60 9.28 1.08 Open in a separate window Mean, average value; SEM, standard error mean; CV%, coefficient of variation; the first five rows were within‐run and the results of total are calculated based on 15 results of the same level The result of daily precision test of two internal quality control (IQC) sera fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Effect of freeze/thaw cycles on two internal quality control ( IQC ) sera A, Time of storage conditions and temperature effect on ACTH assay of BIO ‐ RAD IQC sera B, and of Roche C, at +4°C and room temperature Total precision of Roche IQC was 1.17% and 1.08%, respectively (Table ). .. table ft1 table-wrap mode="anchored" t5 Table 1 caption a7 Mean SEM CV% Bio‐Rad—level 1 20.21 20.08 20.08 20.12 0.08 0.37 15.60 15.49 15.52 15.54 0.06 0.37 14.32 14.53 14.74 14.53 0.21 1.45 14.63 14.59 14.29 14.50 0.19 1.28 15.06 14.79 14.77 14.87 0.16 1.09 Total 15.91 2.22 13.93 Bio‐Rad—level 2 67.51 66.46 66.67 66.88 0.56 0.83 49.32 49.20 49.30 49.27 0.06 0.13 45.70 45.78 45.38 45.62 0.21 0.46 45.41 45.03 44.99 45.14 0.23 0.51 48.22 48.06 47.53 47.94 0.36 0.75 Total 50.97 8.39 16.45 Bio‐Rad—level 3 103.70 103.90 102.89 103.50 0.53 0.52 79.07 78.22 78.56 78.62 0.43 0.54 71.46 71.26 70.29 71.00 0.63 0.88 71.06 71.47 70.62 71.05 0.43 0.60 74.14 73.08 73.80 73.67 0.54 0.73 Total 79.57 12.72 15.98 Roche—level 1 41.96 40.25 40.49 40.90 0.93 2.26 40.03 40.33 40.51 40.29 0.24 0.60 41.21 40.85 41.12 41.06 0.19 0.46 40.25 40.82 40.85 40.64 0.34 0.83 40.89 40.65 40.67 40.74 0.13 0.33 Total 40.73 0.48 1.17 Roche—level 2 864.9 878.5 869.3 870.90 6.94 0.80 861.7 862.9 862.5 862.37 0.61 0.07 857.0 864.2 854.3 858.50 5.12 0.60 856.5 850.8 856.0 854.43 3.16 0.37 841.2 846.6 852.2 846.67 5.50 0.65 Total 858.60 9.28 1.08 Open in a separate window Mean, average value; SEM, standard error mean; CV%, coefficient of variation; the first five rows were within‐run and the results of total are calculated based on 15 results of the same level The result of daily precision test of two internal quality control (IQC) sera fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 Effect of freeze/thaw cycles on two internal quality control ( IQC ) sera A, Time of storage conditions and temperature effect on ACTH assay of BIO ‐ RAD IQC sera B, and of Roche C, at +4°C and room temperature Total precision of Roche IQC was 1.17% and 1.08%, respectively (Table ).
Immunohistochemistry:Article Title: CSF1R-Mediated Myeloid Cell Depletion Prolongs Lifespan But Aggravates Distinct Motor Symptoms in a Model of Multiple System Atrophy
Article Snippet: .. Primary antibodies used for immunohistochemistry, Western blot, and FACS Primary antibody Company RRID number Concentration CD11b-APC eBioscience (17-0112-82) AB_469343 1:100 CD45-A488 BioLegend (103122) AB_493531 1:100 Chicken anti-GFAP Abcam (ab4674) AB_304558 1:400 Goat anti-IBA1 Abcam (ab5076) AB_2224402 1:500 Mouse anti-GAPDH Millipore (MAB374) AB_2107445 1:1000 Rabbit anti-OLIG2 Abcam (ab109186) AB_10861310 1:500 Rabbit anti-PLP Abcam (ab28486) AB_776593 1:1000 Rabbit anti-PU.1 Cell Signaling (2266S) AB_10692379 1:400 Rabbit anti-TH Millipore (AB152) AB_390204 1:500 Rat anti-15G7 Enzo (ALX-804-258) AB_2270759 1:200 Rat anti-BrdU Abd Serotec (OBT0030) AB_2313756 1:500 Rat anti-MBP Abd Serotec (MCA409S) AB_325004 1:500 Table 3. .. Secondary antibodies used for immunohistochemistry and Western blot Secondary antibody Company RRID number Concentration Donkey anti-rabbit A647 Dianova (711-605-152) AB_2492288 1:1000 Donkey anti-rabbit biotin Dianova (711-065-152) AB_2340593 1:1000 Donkey anti-chicken A488 Dianova (703-545-155) AB_2340375 1:1000 Donkey anti-mouse A488 Thermo Fisher Scientific (A21202) AB_141607 1:1000 Donkey anti-rat A488 Thermo Fisher Scientific (A21208) AB_141709 1:1000 Donkey anti-goat A488 Thermo Fisher Scientific (A11055) AB_2534102 1:1000 Goat anti-rat A568 Thermo Fisher Scientific (A11077) AB_2534121 1:1000 Macro used to analyze TH1 fiber length input = getDirectory(“enter dataset”); output = getDirectory(“enter desired output directory”); suffix = “.tif ”; processFolder(input); function processFolder(input) { list = getFileList(input); for (i = 0; i, list.length; i11) { if(File.isDirectory(input1 list[i])) processFolder(““1 input1 list[i]); if(endsWith(list[i], suffix)) processFile(input, output, list[i]); } } setBatchMode(true); function processFile (input, output, file) { open(input1 file); run(“Set Scale...”, “distance= 96 known=20 pixel = 1 unit = mm global”); run(“8-bit”); run(“Auto Threshold,” “method = Otsu white”); run(“Skeletonize (2D/3D)”); run(“Analyze Skeleton (2D/3D)”, “prune = none show”); Table.sort(“Branch length”); print(“Processing: “1 input1 file); print(“Saving to: “1 output); } close(“*”) selectWindow(“Branch information”) saveAs(“Results,” “enter desired folder for data storage”);
Western Blot:Article Title: CSF1R-Mediated Myeloid Cell Depletion Prolongs Lifespan But Aggravates Distinct Motor Symptoms in a Model of Multiple System Atrophy
Article Snippet: .. Primary antibodies used for immunohistochemistry, Western blot, and FACS Primary antibody Company RRID number Concentration CD11b-APC eBioscience (17-0112-82) AB_469343 1:100 CD45-A488 BioLegend (103122) AB_493531 1:100 Chicken anti-GFAP Abcam (ab4674) AB_304558 1:400 Goat anti-IBA1 Abcam (ab5076) AB_2224402 1:500 Mouse anti-GAPDH Millipore (MAB374) AB_2107445 1:1000 Rabbit anti-OLIG2 Abcam (ab109186) AB_10861310 1:500 Rabbit anti-PLP Abcam (ab28486) AB_776593 1:1000 Rabbit anti-PU.1 Cell Signaling (2266S) AB_10692379 1:400 Rabbit anti-TH Millipore (AB152) AB_390204 1:500 Rat anti-15G7 Enzo (ALX-804-258) AB_2270759 1:200 Rat anti-BrdU Abd Serotec (OBT0030) AB_2313756 1:500 Rat anti-MBP Abd Serotec (MCA409S) AB_325004 1:500 Table 3. .. Secondary antibodies used for immunohistochemistry and Western blot Secondary antibody Company RRID number Concentration Donkey anti-rabbit A647 Dianova (711-605-152) AB_2492288 1:1000 Donkey anti-rabbit biotin Dianova (711-065-152) AB_2340593 1:1000 Donkey anti-chicken A488 Dianova (703-545-155) AB_2340375 1:1000 Donkey anti-mouse A488 Thermo Fisher Scientific (A21202) AB_141607 1:1000 Donkey anti-rat A488 Thermo Fisher Scientific (A21208) AB_141709 1:1000 Donkey anti-goat A488 Thermo Fisher Scientific (A11055) AB_2534102 1:1000 Goat anti-rat A568 Thermo Fisher Scientific (A11077) AB_2534121 1:1000 Macro used to analyze TH1 fiber length input = getDirectory(“enter dataset”); output = getDirectory(“enter desired output directory”); suffix = “.tif ”; processFolder(input); function processFolder(input) { list = getFileList(input); for (i = 0; i, list.length; i11) { if(File.isDirectory(input1 list[i])) processFolder(““1 input1 list[i]); if(endsWith(list[i], suffix)) processFile(input, output, list[i]); } } setBatchMode(true); function processFile (input, output, file) { open(input1 file); run(“Set Scale...”, “distance= 96 known=20 pixel = 1 unit = mm global”); run(“8-bit”); run(“Auto Threshold,” “method = Otsu white”); run(“Skeletonize (2D/3D)”); run(“Analyze Skeleton (2D/3D)”, “prune = none show”); Table.sort(“Branch length”); print(“Processing: “1 input1 file); print(“Saving to: “1 output); } close(“*”) selectWindow(“Branch information”) saveAs(“Results,” “enter desired folder for data storage”);
Article Title: Molecular dissection of amyloid disaggregation by human HSP70.
Article Snippet: The deposition of highly ordered fibrillar-type aggregates into inclusion bodies is a hallmark of neurodegenerative diseases such as Parkinson’s disease.. The high stability of such amyloid fibril aggregates makes them challenging substrates for the cellular protein quality-control machinery.. However, the human HSP70 chaperone and its co-chaperones DNAJB1 and HSP110 can dissolve preformed fibrils of the Parkinson’s disease-linked presynaptic protein α-synuclein in vitro.
FACS:Article Title: CSF1R-Mediated Myeloid Cell Depletion Prolongs Lifespan But Aggravates Distinct Motor Symptoms in a Model of Multiple System Atrophy
Article Snippet: .. Primary antibodies used for immunohistochemistry, Western blot, and FACS Primary antibody Company RRID number Concentration CD11b-APC eBioscience (17-0112-82) AB_469343 1:100 CD45-A488 BioLegend (103122) AB_493531 1:100 Chicken anti-GFAP Abcam (ab4674) AB_304558 1:400 Goat anti-IBA1 Abcam (ab5076) AB_2224402 1:500 Mouse anti-GAPDH Millipore (MAB374) AB_2107445 1:1000 Rabbit anti-OLIG2 Abcam (ab109186) AB_10861310 1:500 Rabbit anti-PLP Abcam (ab28486) AB_776593 1:1000 Rabbit anti-PU.1 Cell Signaling (2266S) AB_10692379 1:400 Rabbit anti-TH Millipore (AB152) AB_390204 1:500 Rat anti-15G7 Enzo (ALX-804-258) AB_2270759 1:200 Rat anti-BrdU Abd Serotec (OBT0030) AB_2313756 1:500 Rat anti-MBP Abd Serotec (MCA409S) AB_325004 1:500 Table 3. .. Secondary antibodies used for immunohistochemistry and Western blot Secondary antibody Company RRID number Concentration Donkey anti-rabbit A647 Dianova (711-605-152) AB_2492288 1:1000 Donkey anti-rabbit biotin Dianova (711-065-152) AB_2340593 1:1000 Donkey anti-chicken A488 Dianova (703-545-155) AB_2340375 1:1000 Donkey anti-mouse A488 Thermo Fisher Scientific (A21202) AB_141607 1:1000 Donkey anti-rat A488 Thermo Fisher Scientific (A21208) AB_141709 1:1000 Donkey anti-goat A488 Thermo Fisher Scientific (A11055) AB_2534102 1:1000 Goat anti-rat A568 Thermo Fisher Scientific (A11077) AB_2534121 1:1000 Macro used to analyze TH1 fiber length input = getDirectory(“enter dataset”); output = getDirectory(“enter desired output directory”); suffix = “.tif ”; processFolder(input); function processFolder(input) { list = getFileList(input); for (i = 0; i, list.length; i11) { if(File.isDirectory(input1 list[i])) processFolder(““1 input1 list[i]); if(endsWith(list[i], suffix)) processFile(input, output, list[i]); } } setBatchMode(true); function processFile (input, output, file) { open(input1 file); run(“Set Scale...”, “distance= 96 known=20 pixel = 1 unit = mm global”); run(“8-bit”); run(“Auto Threshold,” “method = Otsu white”); run(“Skeletonize (2D/3D)”); run(“Analyze Skeleton (2D/3D)”, “prune = none show”); Table.sort(“Branch length”); print(“Processing: “1 input1 file); print(“Saving to: “1 output); } close(“*”) selectWindow(“Branch information”) saveAs(“Results,” “enter desired folder for data storage”);
Concentration Assay:Article Title: CSF1R-Mediated Myeloid Cell Depletion Prolongs Lifespan But Aggravates Distinct Motor Symptoms in a Model of Multiple System Atrophy
Article Snippet: .. Primary antibodies used for immunohistochemistry, Western blot, and FACS Primary antibody Company RRID number Concentration CD11b-APC eBioscience (17-0112-82) AB_469343 1:100 CD45-A488 BioLegend (103122) AB_493531 1:100 Chicken anti-GFAP Abcam (ab4674) AB_304558 1:400 Goat anti-IBA1 Abcam (ab5076) AB_2224402 1:500 Mouse anti-GAPDH Millipore (MAB374) AB_2107445 1:1000 Rabbit anti-OLIG2 Abcam (ab109186) AB_10861310 1:500 Rabbit anti-PLP Abcam (ab28486) AB_776593 1:1000 Rabbit anti-PU.1 Cell Signaling (2266S) AB_10692379 1:400 Rabbit anti-TH Millipore (AB152) AB_390204 1:500 Rat anti-15G7 Enzo (ALX-804-258) AB_2270759 1:200 Rat anti-BrdU Abd Serotec (OBT0030) AB_2313756 1:500 Rat anti-MBP Abd Serotec (MCA409S) AB_325004 1:500 Table 3. .. Secondary antibodies used for immunohistochemistry and Western blot Secondary antibody Company RRID number Concentration Donkey anti-rabbit A647 Dianova (711-605-152) AB_2492288 1:1000 Donkey anti-rabbit biotin Dianova (711-065-152) AB_2340593 1:1000 Donkey anti-chicken A488 Dianova (703-545-155) AB_2340375 1:1000 Donkey anti-mouse A488 Thermo Fisher Scientific (A21202) AB_141607 1:1000 Donkey anti-rat A488 Thermo Fisher Scientific (A21208) AB_141709 1:1000 Donkey anti-goat A488 Thermo Fisher Scientific (A11055) AB_2534102 1:1000 Goat anti-rat A568 Thermo Fisher Scientific (A11077) AB_2534121 1:1000 Macro used to analyze TH1 fiber length input = getDirectory(“enter dataset”); output = getDirectory(“enter desired output directory”); suffix = “.tif ”; processFolder(input); function processFolder(input) { list = getFileList(input); for (i = 0; i, list.length; i11) { if(File.isDirectory(input1 list[i])) processFolder(““1 input1 list[i]); if(endsWith(list[i], suffix)) processFile(input, output, list[i]); } } setBatchMode(true); function processFile (input, output, file) { open(input1 file); run(“Set Scale...”, “distance= 96 known=20 pixel = 1 unit = mm global”); run(“8-bit”); run(“Auto Threshold,” “method = Otsu white”); run(“Skeletonize (2D/3D)”); run(“Analyze Skeleton (2D/3D)”, “prune = none show”); Table.sort(“Branch length”); print(“Processing: “1 input1 file); print(“Saving to: “1 output); } close(“*”) selectWindow(“Branch information”) saveAs(“Results,” “enter desired folder for data storage”);
Software:
Infection:Article Title: Estimating HIV Incidence Using a Cross-Sectional Survey: Comparison of Three Approaches in a Hyperendemic Setting, Ndhiwa Subcounty, Kenya, 2012
Article Snippet: A total of 1,346 (93%) of 1,446 HIV-seropositive specimens were tested by both LAg and BioRad; 31 were classified as recent by the LAg-based RITA and 72 were classified as recent by the BioRad-based RITA ( ). .. Among HIV-seropositive individuals, 157 (11%) reported testing HIV negative during the year preceding the survey ( ). table ft1 table-wrap mode="anchored" t5 Table 1. caption a7 Recent infection Acute infection a BioRad-based RITA LAg-based RITA Testing history b Overall 11/4,442 72/1,346 31/1,346 157/1,446 Sex Men 4/1,804 20/420 6/420 42/453 Women 7/2,638 52/926 25/926 115/993 Age groups 15–24 years 8/1,898 20/209 12/209 62/231 25–34 years 2/1,076 25/480 10/480 53/522 35–59 years 1/1,468 27/657 9/657 42/693 Open in a separate window a RNA-positive samples among HIV-seronegative ones. b HIV-seropositive individuals reporting testing HIV negative during the year preceding the survey. ..
other:Article Title: Identification of Piperazinylbenzenesulfonamides as New Inhibitors of Claudin-1 Trafficking and Hepatitis C Virus Entry
Article Snippet: Anti--427 tubulin (T5201), anti-HA tag (16B12), anti E-cadherin (AF648), anti-TGN46 (AHP500G), 428 anti-GM130 (610822) and anti-ERGIC53 (ALX-804-602) antibodies were obtained from 429 Sigma, Covance, R&D Systems, AbD Serotec, BD Biosciences and Enzo Life Sciences, 430 respectively.
Article Title: Chronic Chlamydia infection in human organoids increases stemness and promotes age-dependent CpG methylation
Article Snippet: The following antibodies were used in this study: primary—mouse anti-E-Cadherin (1:200 610181, BD Transduction Lab), mouse anti-β-actin (1:200, A5441, Sigma), goat anti- Ctr (OBT0978, AbD Serotec), mouse anti-HSP60 (1:5000, ALX-804-701, Alexis), rabbit anti-Ki67 (9027, Cell Signaling), rabbit anti-pSTAT1 (1:1000, 9167, Cell Signaling), rabbit anti-pSTAT3 (Tyr705) (1:1000 9145, Cell Signaling), cleaved caspase-3 (1:1000, 9664, Cell Signaling), goat anti-LIF (1:500, AF-250-NA, R&D Systems); secondary - sheep anti-mouse-HRP (1:3000, NA931, Amersham), donkey anti-rabbit-HRP (1:3000, NA934, Amersham), donkey anti-goat-HRP (1:3000, 800073, Biomol), donkey anti-mouse-Alexa488 (1:300, 715-546-140, Dianova), donkey anti-goat-Cy3 (1:300, 705-165-003, Dianova), donkey anti-rabbit-Alexa488 (1:300, 711-546-152, Dianova), donkey anti-mouse-Dylight 647 (1:300, 715-605-150, Dianova), CD326 (EpCAM)-FITC antibody (1:50, 130-080-301, Miltenyi), mouse anti-human CD24-BV711 antibody (1:200, 563371, BD Biosciences), mouse anti IgG1-APC (1:100, 130-098-846, Miltenyi), and mouse anti-human CD133/1 (AC133) APC (1:100, 130 098 829, Miltenyi).
Chromatin Immunoprecipitation:Article Title: Molecular dissection of amyloid disaggregation by human HSP70.
Article Snippet: The deposition of highly ordered fibrillar-type aggregates into inclusion bodies is a hallmark of neurodegenerative diseases such as Parkinson’s disease.. The high stability of such amyloid fibril aggregates makes them challenging substrates for the cellular protein quality-control machinery.. However, the human HSP70 chaperone and its co-chaperones DNAJB1 and HSP110 can dissolve preformed fibrils of the Parkinson’s disease-linked presynaptic protein α-synuclein in vitro.
Flow Cytometry:Article Title: Molecular dissection of amyloid disaggregation by human HSP70.
Article Snippet: The deposition of highly ordered fibrillar-type aggregates into inclusion bodies is a hallmark of neurodegenerative diseases such as Parkinson’s disease.. The high stability of such amyloid fibril aggregates makes them challenging substrates for the cellular protein quality-control machinery.. However, the human HSP70 chaperone and its co-chaperones DNAJB1 and HSP110 can dissolve preformed fibrils of the Parkinson’s disease-linked presynaptic protein α-synuclein in vitro.
Magnetic Resonance Imaging:Article Title: Molecular dissection of amyloid disaggregation by human HSP70.
Article Snippet: The deposition of highly ordered fibrillar-type aggregates into inclusion bodies is a hallmark of neurodegenerative diseases such as Parkinson’s disease.. The high stability of such amyloid fibril aggregates makes them challenging substrates for the cellular protein quality-control machinery.. However, the human HSP70 chaperone and its co-chaperones DNAJB1 and HSP110 can dissolve preformed fibrils of the Parkinson’s disease-linked presynaptic protein α-synuclein in vitro.
Biomarker Discovery:Article Title: Molecular dissection of amyloid disaggregation by human HSP70.
Article Snippet: The deposition of highly ordered fibrillar-type aggregates into inclusion bodies is a hallmark of neurodegenerative diseases such as Parkinson’s disease.. The high stability of such amyloid fibril aggregates makes them challenging substrates for the cellular protein quality-control machinery.. However, the human HSP70 chaperone and its co-chaperones DNAJB1 and HSP110 can dissolve preformed fibrils of the Parkinson’s disease-linked presynaptic protein α-synuclein in vitro.
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