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matchmaker insert check pcr mix 2  (TaKaRa)


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    Structured Review

    TaKaRa matchmaker insert check pcr mix 2
    Matchmaker Insert Check Pcr Mix 2, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 71 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/insert/Matchmaker+Insert+Check+PCR+Mix+2/pmc06440577-181-1-7
    Average 94 stars, based on 71 article reviews
    matchmaker insert check pcr mix 2 - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: HbTRXh1 regulates tapping panel dryness occurrence and abiotic stress tolerance by interacting with stress-responsive proteins in rubber tree.
    Article Snippet: .. PCR amplification was conducted with Matchmaker Insert Check PCR Mix 2 (Clontech, California, USA) using the blue colonies as templates. ..

    Article Title: Transcription factor ZjNF-YC9 regulates ZjGME1 to promote ascorbic acid accumulation in jujube fruits
    Article Snippet: .. Under AbA selection, yeast colonies showing positive growth were subjected to analysis with Matchmaker Insert Check PCR Mix 2 (TaKaRa) and sequencing, leading to the identification of a putative transcription factor, ZjNF-YC9 (Zj.jz037921106, nuclear transcription factor Y [NF-Y] subunit C-9). ..

    Article Title: Integration of transcriptomics and metabolomics reveals the regulation of (E)-β-ionone synthesis by the CsKHZ1-CsCCD4 module in Chinese orchid (Cymbidium sinense)
    Article Snippet: .. After 3 days of incubation at 30◦C, positive clones were amplified by colony PCR using Matchmaker Insert Check PCR Mix 2 (Takara, Tokyo, Japan). .. PCR products were sequenced using the T7 primer on a Sanger sequencing platform (Sangon Biotech, Shanghai, China) to identify potential transcriptional regulators.

    Amplification:

    Article Title: HbTRXh1 regulates tapping panel dryness occurrence and abiotic stress tolerance by interacting with stress-responsive proteins in rubber tree.
    Article Snippet: .. PCR amplification was conducted with Matchmaker Insert Check PCR Mix 2 (Clontech, California, USA) using the blue colonies as templates. ..

    Article Title: Integration of transcriptomics and metabolomics reveals the regulation of (E)-β-ionone synthesis by the CsKHZ1-CsCCD4 module in Chinese orchid (Cymbidium sinense)
    Article Snippet: .. After 3 days of incubation at 30◦C, positive clones were amplified by colony PCR using Matchmaker Insert Check PCR Mix 2 (Takara, Tokyo, Japan). .. PCR products were sequenced using the T7 primer on a Sanger sequencing platform (Sangon Biotech, Shanghai, China) to identify potential transcriptional regulators.

    other:

    Article Title: Construction of Yeast One-Hybrid Library of Dendrobium huoshanense and Screening of Potential Transcription Factors Regulating DhPMM Gene Expression
    Article Snippet: Surviving clones were cultured in SD/-Leu liquid medium at OD 600 = 0.6–0.8 and validated by the Matchmaker Insert Check PCR Mix 2 (630497, Takara).

    Article Title: Construction of Yeast One-Hybrid Library of Dendrobium huoshanense and Screening of Potential Transcription Factors Regulating DhPMM Gene Expression
    Article Snippet: Surviving clones were cultured in SD/-Leu liquid medium at OD600 = 0.6–0.8 and validated by the Matchmaker Insert Check PCR Mix 2 (630497, Takara).

    Article Title: Identification of host proteins interacting with NS3 protein of Bluetongue virus
    Article Snippet: The library’s average insert size and recombination rate were calculated from randomly selected 50 colonies using Matchmaker Insert Check PCR Mix 2 (Clontech, Takara, USA) as described earlier (Mahajan et al., 2015).

    Article Title: The PopGRF3-PopKNAT3 module orchestrates salt tolerance through transcriptional control of stress-responsive genes in poplar.
    Article Snippet: Plants are often exposed to various stressors during their growth and development.. Sustaining intracellular ion equilibrium, especially the balance between Na and K, plays a vital role in plant adaptation to salt stress.. In this study, we identified growth-regulating factor 3 (PopGRF3), a gene encoding a transcription factor (TF) that acts as a negative regulator of salt stress responses.

    Selection:

    Article Title: Transcription factor ZjNF-YC9 regulates ZjGME1 to promote ascorbic acid accumulation in jujube fruits
    Article Snippet: .. Under AbA selection, yeast colonies showing positive growth were subjected to analysis with Matchmaker Insert Check PCR Mix 2 (TaKaRa) and sequencing, leading to the identification of a putative transcription factor, ZjNF-YC9 (Zj.jz037921106, nuclear transcription factor Y [NF-Y] subunit C-9). ..

    Yeast:

    Article Title: Transcription factor ZjNF-YC9 regulates ZjGME1 to promote ascorbic acid accumulation in jujube fruits
    Article Snippet: .. Under AbA selection, yeast colonies showing positive growth were subjected to analysis with Matchmaker Insert Check PCR Mix 2 (TaKaRa) and sequencing, leading to the identification of a putative transcription factor, ZjNF-YC9 (Zj.jz037921106, nuclear transcription factor Y [NF-Y] subunit C-9). ..

    Analysis:

    Article Title: Transcription factor ZjNF-YC9 regulates ZjGME1 to promote ascorbic acid accumulation in jujube fruits
    Article Snippet: .. Under AbA selection, yeast colonies showing positive growth were subjected to analysis with Matchmaker Insert Check PCR Mix 2 (TaKaRa) and sequencing, leading to the identification of a putative transcription factor, ZjNF-YC9 (Zj.jz037921106, nuclear transcription factor Y [NF-Y] subunit C-9). ..

    Sequencing:

    Article Title: Transcription factor ZjNF-YC9 regulates ZjGME1 to promote ascorbic acid accumulation in jujube fruits
    Article Snippet: .. Under AbA selection, yeast colonies showing positive growth were subjected to analysis with Matchmaker Insert Check PCR Mix 2 (TaKaRa) and sequencing, leading to the identification of a putative transcription factor, ZjNF-YC9 (Zj.jz037921106, nuclear transcription factor Y [NF-Y] subunit C-9). ..

    Incubation:

    Article Title: Integration of transcriptomics and metabolomics reveals the regulation of (E)-β-ionone synthesis by the CsKHZ1-CsCCD4 module in Chinese orchid (Cymbidium sinense)
    Article Snippet: .. After 3 days of incubation at 30◦C, positive clones were amplified by colony PCR using Matchmaker Insert Check PCR Mix 2 (Takara, Tokyo, Japan). .. PCR products were sequenced using the T7 primer on a Sanger sequencing platform (Sangon Biotech, Shanghai, China) to identify potential transcriptional regulators.

    Clone Assay:

    Article Title: Integration of transcriptomics and metabolomics reveals the regulation of (E)-β-ionone synthesis by the CsKHZ1-CsCCD4 module in Chinese orchid (Cymbidium sinense)
    Article Snippet: .. After 3 days of incubation at 30◦C, positive clones were amplified by colony PCR using Matchmaker Insert Check PCR Mix 2 (Takara, Tokyo, Japan). .. PCR products were sequenced using the T7 primer on a Sanger sequencing platform (Sangon Biotech, Shanghai, China) to identify potential transcriptional regulators.



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    94
    MACHEREY NAGEL compatible snap ring closures
    a Medium from freshly isolated organoids analysed by proteome array. n = 4 mice per group, pooled. Analysis: one-way ANOVA with Tukey’s correction. In bold: known NF-κB target genes. b Treg cell migration <t>transwell</t> assay: isolated MLN/ spleen primary Treg cells from Wt mice incubated with factors shown. NC (negative control – medium alone) or PC (positive control – Fetal bovine serum). Analysis: one-way ANOVA; Bonferroni’s multiple comparison test. Mean and SD shown. n = 9. c IHC against CCL22 ( n = 5 mice). Black triangles: CCL22+ immune cells. Purple triangles: CCL22+ epithelial cells. Scale: 10 μm. d Primary Treg cells from Wt mice cultured with organoid conditioned medium from Wt vs CCL22 −/− mice ( n = 3 mice), treated with LPS (25 μg/ml LPS over 48 h) or left untreated. Treg cell migration quantified via Transwell migration assay. Analysis: one-way ANOVA. Mean and SD shown. e Treg cell co-culture with conditioned organoid medium (SN), 30 Vol.-% and/or + 30 ng/mL murine IL-33, CM + 30 ng/mL IL-33 or CM + 100 ng/mL Amphiregulin. The fold change increase of expanded Treg cells on day 3 was measured in relation to the initial cell count. n = 3 mice. Analysis: as in a. Mean and SD shown. f Quantitation of CCR4+ and FoxP3+ cells from IF per image. n = 9. Mean and SD shown. g Isolated Wt ( n = 5) and IκBα IEC KO ( n = 3) CD4 + CD25+ Treg cells cultured for 6 h in medium supplemented with 50 ng/mL PMA and 1 µg/mL ionomycin. Supernatants analysed by ELISA. Analysis: unpaired t-test, two-sided. Mean and SD shown. h scRNA of colonic versus splenic immune cells from Wt and IκBα IEC KO mice. i Genes with significant changes in expression between colonic or splenic Treg cells in Wt versus IκBα IEC KO mice. j Differential expression of Gdpd3 and Ftl1 in spleen and colon Treg cells. Analysis: two-sided Wilcoxon rank-sum test k Colonic IF against GDPD3, CD4 and CDH1. Scale: 50 μm. n = 12. Zoom: double positive cells. l Quantitation of GDPD3+CD4+ cells per image. Analysis as in ( a ). Mean and SD shown. m Primary Treg cells from Wt mice cultured with organoid SN (supernatants). n = 3 mice. GDPD3+ cells visualized by IF. Analysis: as in ( a ). Mean and SD shown. Source data are provided as a Source Data file.
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    a Medium from freshly isolated organoids analysed by proteome array. n = 4 mice per group, pooled. Analysis: one-way ANOVA with Tukey’s correction. In bold: known NF-κB target genes. b Treg cell migration transwell assay: isolated MLN/ spleen primary Treg cells from Wt mice incubated with factors shown. NC (negative control – medium alone) or PC (positive control – Fetal bovine serum). Analysis: one-way ANOVA; Bonferroni’s multiple comparison test. Mean and SD shown. n = 9. c IHC against CCL22 ( n = 5 mice). Black triangles: CCL22+ immune cells. Purple triangles: CCL22+ epithelial cells. Scale: 10 μm. d Primary Treg cells from Wt mice cultured with organoid conditioned medium from Wt vs CCL22 −/− mice ( n = 3 mice), treated with LPS (25 μg/ml LPS over 48 h) or left untreated. Treg cell migration quantified via Transwell migration assay. Analysis: one-way ANOVA. Mean and SD shown. e Treg cell co-culture with conditioned organoid medium (SN), 30 Vol.-% and/or + 30 ng/mL murine IL-33, CM + 30 ng/mL IL-33 or CM + 100 ng/mL Amphiregulin. The fold change increase of expanded Treg cells on day 3 was measured in relation to the initial cell count. n = 3 mice. Analysis: as in a. Mean and SD shown. f Quantitation of CCR4+ and FoxP3+ cells from IF per image. n = 9. Mean and SD shown. g Isolated Wt ( n = 5) and IκBα IEC KO ( n = 3) CD4 + CD25+ Treg cells cultured for 6 h in medium supplemented with 50 ng/mL PMA and 1 µg/mL ionomycin. Supernatants analysed by ELISA. Analysis: unpaired t-test, two-sided. Mean and SD shown. h scRNA of colonic versus splenic immune cells from Wt and IκBα IEC KO mice. i Genes with significant changes in expression between colonic or splenic Treg cells in Wt versus IκBα IEC KO mice. j Differential expression of Gdpd3 and Ftl1 in spleen and colon Treg cells. Analysis: two-sided Wilcoxon rank-sum test k Colonic IF against GDPD3, CD4 and CDH1. Scale: 50 μm. n = 12. Zoom: double positive cells. l Quantitation of GDPD3+CD4+ cells per image. Analysis as in ( a ). Mean and SD shown. m Primary Treg cells from Wt mice cultured with organoid SN (supernatants). n = 3 mice. GDPD3+ cells visualized by IF. Analysis: as in ( a ). Mean and SD shown. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Epithelial NF-κB shapes protective Treg cell responses to restrain intestinal inflammation

    doi: 10.1038/s41467-026-78796-z

    Figure Lengend Snippet: a Medium from freshly isolated organoids analysed by proteome array. n = 4 mice per group, pooled. Analysis: one-way ANOVA with Tukey’s correction. In bold: known NF-κB target genes. b Treg cell migration transwell assay: isolated MLN/ spleen primary Treg cells from Wt mice incubated with factors shown. NC (negative control – medium alone) or PC (positive control – Fetal bovine serum). Analysis: one-way ANOVA; Bonferroni’s multiple comparison test. Mean and SD shown. n = 9. c IHC against CCL22 ( n = 5 mice). Black triangles: CCL22+ immune cells. Purple triangles: CCL22+ epithelial cells. Scale: 10 μm. d Primary Treg cells from Wt mice cultured with organoid conditioned medium from Wt vs CCL22 −/− mice ( n = 3 mice), treated with LPS (25 μg/ml LPS over 48 h) or left untreated. Treg cell migration quantified via Transwell migration assay. Analysis: one-way ANOVA. Mean and SD shown. e Treg cell co-culture with conditioned organoid medium (SN), 30 Vol.-% and/or + 30 ng/mL murine IL-33, CM + 30 ng/mL IL-33 or CM + 100 ng/mL Amphiregulin. The fold change increase of expanded Treg cells on day 3 was measured in relation to the initial cell count. n = 3 mice. Analysis: as in a. Mean and SD shown. f Quantitation of CCR4+ and FoxP3+ cells from IF per image. n = 9. Mean and SD shown. g Isolated Wt ( n = 5) and IκBα IEC KO ( n = 3) CD4 + CD25+ Treg cells cultured for 6 h in medium supplemented with 50 ng/mL PMA and 1 µg/mL ionomycin. Supernatants analysed by ELISA. Analysis: unpaired t-test, two-sided. Mean and SD shown. h scRNA of colonic versus splenic immune cells from Wt and IκBα IEC KO mice. i Genes with significant changes in expression between colonic or splenic Treg cells in Wt versus IκBα IEC KO mice. j Differential expression of Gdpd3 and Ftl1 in spleen and colon Treg cells. Analysis: two-sided Wilcoxon rank-sum test k Colonic IF against GDPD3, CD4 and CDH1. Scale: 50 μm. n = 12. Zoom: double positive cells. l Quantitation of GDPD3+CD4+ cells per image. Analysis as in ( a ). Mean and SD shown. m Primary Treg cells from Wt mice cultured with organoid SN (supernatants). n = 3 mice. GDPD3+ cells visualized by IF. Analysis: as in ( a ). Mean and SD shown. Source data are provided as a Source Data file.

    Article Snippet: 4.5 × 10 4 cells/well were seeded in RPMI medium without additives on a transwell insert (8μm pore size, Greiner Bio-One Cat. 662638) in a 24- well plate.

    Techniques: Isolation, Organoids, Analysis, Migration, Transwell Assay, Incubation, Negative Control, Positive Control, Comparison, Cell Culture, Transwell Migration Assay, Co-culture Assay, Cell Characterization, Quantitation Assay, Enzyme-linked Immunosorbent Assay, Expressing, Quantitative Proteomics

    a scRNA dataset ( GSE116222 ) is based on samples from inflamed and non-inflamed colon regions of UC patients, as well as those from healthy controls. Visualization of IEC cells across the three conditions. b TRRUST analysis of top upregulated TF networks across the three conditions (Healthy, UC and UC NI (non-inflamed)) in IEC presented as a volcano plot. Text in blue: factors belonging to the NF-κB axis. A Gene-set enrichment was performed using Enrichr. Enrichment significance was assessed using Fisher’s exact test, and enrichment magnitude was represented by the odds ratio. Volcano plots generated with the Enrichr Appyter display odds ratio versus −log 10 ( P value). c TRRUST analysis of downregulated TF networks in UC-inflamed colon IEC versus healthy IEC. Text in blue: factors belonging to the NF-κB axis. Analysis as in ( b ). d H&E of colon sections from healthy and UC patients. Scale: 50 μm. e Colitis score (Nancy index) from ( d ). The patient number is shown in the graph. Analysis: Two-tailed t -test. Mean and SD shown. f Colon-derived organoids were derived from colon biopsies of UC patients or non-IBD controls, with at least n = 4 patients per group. Growth of colon organoids was measured over several days. Mean and SD shown. Analysis: t- test, two-tailed, unpaired. g At least 10 colon organoids per patient from healthy ( n = 3 persons) and UC colon-derived organoids ( n = 4 patients) treated with LPS over 48 h and stained with antibodies as indicated. Scale: 50 μm. h Quantification from g. Top panel: Ki67 (not significant). Lower panel: cleaved caspase 3. Analysis: One-way ANOVA with Tukey’s correction. Mean and SD shown. i Colon organoids were treated with TNF (TNF was removed, and the medium was changed) or left untreated. Medium from colon-derived organoids was analysed by proteome array, and the presence of secreted factors quantitated from bulk ( n = 4) samples per group and n = 2 technical replicates. Significance determined by one-way ANOVA with Tukey’s correction. Only significant results are shown. j Analysis of proteome data by DAVID v2023q4 followed by REVIGO for visualization of GO terms on semantic space. Bubble size indicates prevalence. Color: orange—terms enriched in medium of UC colon-derived organoids treated with TNF, purple—terms enriched in medium of healthy colon-derived organoids treated with TNF. SD and mean shown. Analysis: Welch’s t -test, two-sided – no significance. k Transwell recruitment with Jurkat cells incubated in medium from colon-derived organoids from UC patients or healthy controls. Analysis: one-way ANOVA with Tukey’s correction. n = 4 for healthy samples and n = 6 for UC samples (pooled). Individual points indicate technical replicates. Mean and SD shown. Source data are provided as a Source Data file.

    Journal: Nature Communications

    Article Title: Epithelial NF-κB shapes protective Treg cell responses to restrain intestinal inflammation

    doi: 10.1038/s41467-026-78796-z

    Figure Lengend Snippet: a scRNA dataset ( GSE116222 ) is based on samples from inflamed and non-inflamed colon regions of UC patients, as well as those from healthy controls. Visualization of IEC cells across the three conditions. b TRRUST analysis of top upregulated TF networks across the three conditions (Healthy, UC and UC NI (non-inflamed)) in IEC presented as a volcano plot. Text in blue: factors belonging to the NF-κB axis. A Gene-set enrichment was performed using Enrichr. Enrichment significance was assessed using Fisher’s exact test, and enrichment magnitude was represented by the odds ratio. Volcano plots generated with the Enrichr Appyter display odds ratio versus −log 10 ( P value). c TRRUST analysis of downregulated TF networks in UC-inflamed colon IEC versus healthy IEC. Text in blue: factors belonging to the NF-κB axis. Analysis as in ( b ). d H&E of colon sections from healthy and UC patients. Scale: 50 μm. e Colitis score (Nancy index) from ( d ). The patient number is shown in the graph. Analysis: Two-tailed t -test. Mean and SD shown. f Colon-derived organoids were derived from colon biopsies of UC patients or non-IBD controls, with at least n = 4 patients per group. Growth of colon organoids was measured over several days. Mean and SD shown. Analysis: t- test, two-tailed, unpaired. g At least 10 colon organoids per patient from healthy ( n = 3 persons) and UC colon-derived organoids ( n = 4 patients) treated with LPS over 48 h and stained with antibodies as indicated. Scale: 50 μm. h Quantification from g. Top panel: Ki67 (not significant). Lower panel: cleaved caspase 3. Analysis: One-way ANOVA with Tukey’s correction. Mean and SD shown. i Colon organoids were treated with TNF (TNF was removed, and the medium was changed) or left untreated. Medium from colon-derived organoids was analysed by proteome array, and the presence of secreted factors quantitated from bulk ( n = 4) samples per group and n = 2 technical replicates. Significance determined by one-way ANOVA with Tukey’s correction. Only significant results are shown. j Analysis of proteome data by DAVID v2023q4 followed by REVIGO for visualization of GO terms on semantic space. Bubble size indicates prevalence. Color: orange—terms enriched in medium of UC colon-derived organoids treated with TNF, purple—terms enriched in medium of healthy colon-derived organoids treated with TNF. SD and mean shown. Analysis: Welch’s t -test, two-sided – no significance. k Transwell recruitment with Jurkat cells incubated in medium from colon-derived organoids from UC patients or healthy controls. Analysis: one-way ANOVA with Tukey’s correction. n = 4 for healthy samples and n = 6 for UC samples (pooled). Individual points indicate technical replicates. Mean and SD shown. Source data are provided as a Source Data file.

    Article Snippet: 4.5 × 10 4 cells/well were seeded in RPMI medium without additives on a transwell insert (8μm pore size, Greiner Bio-One Cat. 662638) in a 24- well plate.

    Techniques: Analysis, Generated, Two Tailed Test, Derivative Assay, Organoids, Staining, Incubation, Significance Assay