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TriPath Imaging sureslide® preparation buffer
Sureslide® Preparation Buffer, supplied by TriPath Imaging, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/imaging+buffer/sureslide++preparation+buffer/us07455973-281-18-21
Average 90 stars, based on 1 article reviews
sureslide® preparation buffer - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: In a particular aspect of the immunocytochemistry methods, slides are transferred to a pretreatment buffer, for example the SureSlide® Preparation Buffer (TriPath Imaging, Inc.) and optionally heated to increase antigen accessibility.

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: In a particular aspect of the immunocytochemistry methods, slides are transferred to a pretreatment buffer, for example the SureSlide® Preparation Buffer (TriPath Imaging, Inc.) and optionally heated to increase antigen accessibility.

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: In a particular aspect of the immunocytochemistry methods, slides are transferred to a pretreatment buffer, for example the SureSlide® Preparation Buffer (TriPath Imaging, Inc.) and optionally heated to increase antigen accessibility.

Bioprocessing:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Polymer:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Saline:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Immunocytochemistry:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Article Title: American Society of Cytopathology, 53rd Annual Scientific Meeting, November 4-9, 2005, San Diego, California, USA. Abstracts.
Article Snippet: .. Pretreatment of the slides was performed by soaking the slides in Slide Pretreatment Buffer (TriPath Imaging) for at least 1 hour and then heating the slides for 15 minutes at 95 C prior to the execution of the ICC assay. ..

Light Microscopy:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Transferring:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Staining:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.

Negative Control:

Article Title: Methods and compositions for the detection of cervical disease
Article Snippet: .. 3a 1 × 15 mL Mouse Probe Reagent: Binds to mouse monoclonal antibodies 3b 1 × 15 mL Polymer Reagent: Polymer conjugated with horseradish peroxidase that binds to Mouse Probe Reagent 4a 1 × 18 mL DAB Substrate Buffer: Substrate buffer used in the preparation of the DAB Chromogen 4b 1 × 1 mL DAB Chromogen: 3,3′- diaminobenzidine chromogen solution 5 1 × 18 mL Hematoxylin Counterstain: aqueous based Mayers Hematoxylin 6 1 × 18 mL Bluing Agent: Tris buffered saline, pH 7.4 with Tween 20 and 0.09% NaN3 The following materials and reagents were required to perform the immunocytochemistry methods but were not supplied in the kit: Absorbent Wipes SiHa Cell Line (TriPath Imaging, Inc.) Deionized or Distilled Water Ethanol (95% and 100%) Glass Coverslips Gloves Humid Chamber Light Microscope (10×, 20×, 40× objectives) Mounting Media Pipettes and Pipette Tips (capable of delivering 20 μl, 200 μl and 1000 μl volumes) SureSlide Preparation Buffer (TriPath Imaging, Inc.)-Pretreatment Buffer (0.5% sodium laureth-13-carboxylate (Sandopan LS) in deionized H2O) Staining Jars or Baths Timer (capable of 1–60 minute intervals) Tris Buffered Saline (TBS) Tween 20 Universal Mouse IgG Negative Control Vortexer Xylene or Xylene Substitutes Steamer/waterbath III. .. Instructions for Use Specimen Preparation The following steps were followed for the preparation of cervical samples: Consult the Operator's Manual for the SurePath PrepStain SystemTM for the preparation of slides from residual specimens.



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a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Journal: bioRxiv

Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

doi: 10.64898/2026.03.30.715210

Figure Lengend Snippet: a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: In brief, cells were washed with the Ca 2+ imaging buffer (1 x HBSS buffer with 1.3 mM Ca 2+ , 10 mM HEPES, pH 7.2) and then loaded with the Ca 2+ imaging buffer containing 2.5 mM Ca 2+ indicator dye Fura-2, AM (ThermoFisher scientific, F1225) and 0.05% Pluronic F-127 (Beyotime, ST501).

Techniques: CRISPR, Knock-Out, Injection, Virus, Construct, Infection, Single Cell, Imaging, Derivative Assay, Control, Cell Culture, Randall–Selitto Test, Hot Plate Test