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imag buffer, becton dickinson  (Becton Dickinson)

 
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    Becton Dickinson imag buffer, becton dickinson
    Imag Buffer, Becton Dickinson, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/imaging+buffer/imag+buffer++becton+dickinson/us09574003-285-201-208
    Average 90 stars, based on 1 article reviews
    imag buffer, becton dickinson - by Bioz Stars, 2026-10
    90/100 stars

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    FACS:

    Article Title: Low fucose cell lines and uses thereof
    Article Snippet: Bovogen Cat. # BSAS.01; Cell Boost, HyClone, Cat. # SH30866.01; CHO CD EfficientFeedTM A, Invitrogen, Cat. # A10234-01 CHO CD EfficientFeedTM B, Invitrogen, Cat. # A10240-01 Citric acid, Sigma Cat. # C-1909; Dextrane sulfate sodium salt, Sigma, Cat. # D4911; DH5α competent bacteria—Life-Technologies Cat. #18263-012; DMSO, Merck, Cat. #1.02931.1000; DNA ladder: 1 kb ladder for DNA—Biolabs Cat No. #3232L; DNA ladder: 100 bp ladder for DNA—Biolabs Cat No. #3231L; DTT, Cat: D9779, Sigma; EDTA, Sigma, Cat. # E7889; Ethanol Merck Cat. #00983.1000; EZ-Link® NHS-PEG4-Biotin, No-WeighTM Format, cat: 21329, Thermo Fisher Scientific Inc USA; FACS Accudrop Beads, Becton Dickinson, Cat. # MAB345249; Fucose, Sigma, Cat. # F2252; Glucose, Sigma, Cat. # G7021; Glutamine, Biological Industries, Cat. #03-020-1A; Glutamine, Sigma, Cat. # G5972; Glycine, Merck, Cat. #4201; Guanidin-HCl, cat: G3272, Sigma; Hepes 1M, Invitrogen, Cat. #15630-056; High Capacity cDNA Reverse Transcription Kit, Applied Biosystems Cat. #4368814; HTX50, Biological Industries, Cat. #03-085-1C; Hydrochloric acid, Merck, Cat. # UN-1789 1.00317.2500; iCE 280 methyl cellulose 1%, Cat.#102220 and 101876, Convergent Biosciences, (Canada); iCE 280 pharmalyte 3-10, Cat. #10-0456-01, GE Healthcare (Germany); iCE 280 pI markers 6.14 and 9.5, Cat. #102220 and 101996 respectively, Convergent Biosciences, (Canada); iCE 280 system suitability kit, Cat. #102093-j, Convergent Biosciences, (Canada); iCE280 IEF Kit Convergent Biosciences; IMag buffer, Becton Dickinson, Cat. #552362; IMag SA Particles Plus—DM, Becton Dickinson, Cat. #557812; Iodoacetamide, cat: I1149, Sigma; ITSX100, Invitrogen, Cat. #51500-056; LB+Ampicillin plates—Hy-Labs Cat. #PD 178; L-Cysteine, Cat: C7352, Sigma; LipofectAmine, Invitrogen, Cat #50470; Maackia amurensis agglutinin 1 mg/ml, cat: L8025, Lot: 036k4075 Sigma; MAA-FITC, 2 mg in 2 ml buffer, EY, Cat. # F-7801-2; Maleimide, cat: 129585, Lot: S56783-278 Sigma; Na2PO4.

    Reverse Transcription:

    Article Title: Low fucose cell lines and uses thereof
    Article Snippet: Bovogen Cat. # BSAS.01; Cell Boost, HyClone, Cat. # SH30866.01; CHO CD EfficientFeedTM A, Invitrogen, Cat. # A10234-01 CHO CD EfficientFeedTM B, Invitrogen, Cat. # A10240-01 Citric acid, Sigma Cat. # C-1909; Dextrane sulfate sodium salt, Sigma, Cat. # D4911; DH5α competent bacteria—Life-Technologies Cat. #18263-012; DMSO, Merck, Cat. #1.02931.1000; DNA ladder: 1 kb ladder for DNA—Biolabs Cat No. #3232L; DNA ladder: 100 bp ladder for DNA—Biolabs Cat No. #3231L; DTT, Cat: D9779, Sigma; EDTA, Sigma, Cat. # E7889; Ethanol Merck Cat. #00983.1000; EZ-Link® NHS-PEG4-Biotin, No-WeighTM Format, cat: 21329, Thermo Fisher Scientific Inc USA; FACS Accudrop Beads, Becton Dickinson, Cat. # MAB345249; Fucose, Sigma, Cat. # F2252; Glucose, Sigma, Cat. # G7021; Glutamine, Biological Industries, Cat. #03-020-1A; Glutamine, Sigma, Cat. # G5972; Glycine, Merck, Cat. #4201; Guanidin-HCl, cat: G3272, Sigma; Hepes 1M, Invitrogen, Cat. #15630-056; High Capacity cDNA Reverse Transcription Kit, Applied Biosystems Cat. #4368814; HTX50, Biological Industries, Cat. #03-085-1C; Hydrochloric acid, Merck, Cat. # UN-1789 1.00317.2500; iCE 280 methyl cellulose 1%, Cat.#102220 and 101876, Convergent Biosciences, (Canada); iCE 280 pharmalyte 3-10, Cat. #10-0456-01, GE Healthcare (Germany); iCE 280 pI markers 6.14 and 9.5, Cat. #102220 and 101996 respectively, Convergent Biosciences, (Canada); iCE 280 system suitability kit, Cat. #102093-j, Convergent Biosciences, (Canada); iCE280 IEF Kit Convergent Biosciences; IMag buffer, Becton Dickinson, Cat. #552362; IMag SA Particles Plus—DM, Becton Dickinson, Cat. #557812; Iodoacetamide, cat: I1149, Sigma; ITSX100, Invitrogen, Cat. #51500-056; LB+Ampicillin plates—Hy-Labs Cat. #PD 178; L-Cysteine, Cat: C7352, Sigma; LipofectAmine, Invitrogen, Cat #50470; Maackia amurensis agglutinin 1 mg/ml, cat: L8025, Lot: 036k4075 Sigma; MAA-FITC, 2 mg in 2 ml buffer, EY, Cat. # F-7801-2; Maleimide, cat: 129585, Lot: S56783-278 Sigma; Na2PO4.

    Electrofocusing:

    Article Title: Low fucose cell lines and uses thereof
    Article Snippet: Bovogen Cat. # BSAS.01; Cell Boost, HyClone, Cat. # SH30866.01; CHO CD EfficientFeedTM A, Invitrogen, Cat. # A10234-01 CHO CD EfficientFeedTM B, Invitrogen, Cat. # A10240-01 Citric acid, Sigma Cat. # C-1909; Dextrane sulfate sodium salt, Sigma, Cat. # D4911; DH5α competent bacteria—Life-Technologies Cat. #18263-012; DMSO, Merck, Cat. #1.02931.1000; DNA ladder: 1 kb ladder for DNA—Biolabs Cat No. #3232L; DNA ladder: 100 bp ladder for DNA—Biolabs Cat No. #3231L; DTT, Cat: D9779, Sigma; EDTA, Sigma, Cat. # E7889; Ethanol Merck Cat. #00983.1000; EZ-Link® NHS-PEG4-Biotin, No-WeighTM Format, cat: 21329, Thermo Fisher Scientific Inc USA; FACS Accudrop Beads, Becton Dickinson, Cat. # MAB345249; Fucose, Sigma, Cat. # F2252; Glucose, Sigma, Cat. # G7021; Glutamine, Biological Industries, Cat. #03-020-1A; Glutamine, Sigma, Cat. # G5972; Glycine, Merck, Cat. #4201; Guanidin-HCl, cat: G3272, Sigma; Hepes 1M, Invitrogen, Cat. #15630-056; High Capacity cDNA Reverse Transcription Kit, Applied Biosystems Cat. #4368814; HTX50, Biological Industries, Cat. #03-085-1C; Hydrochloric acid, Merck, Cat. # UN-1789 1.00317.2500; iCE 280 methyl cellulose 1%, Cat.#102220 and 101876, Convergent Biosciences, (Canada); iCE 280 pharmalyte 3-10, Cat. #10-0456-01, GE Healthcare (Germany); iCE 280 pI markers 6.14 and 9.5, Cat. #102220 and 101996 respectively, Convergent Biosciences, (Canada); iCE 280 system suitability kit, Cat. #102093-j, Convergent Biosciences, (Canada); iCE280 IEF Kit Convergent Biosciences; IMag buffer, Becton Dickinson, Cat. #552362; IMag SA Particles Plus—DM, Becton Dickinson, Cat. #557812; Iodoacetamide, cat: I1149, Sigma; ITSX100, Invitrogen, Cat. #51500-056; LB+Ampicillin plates—Hy-Labs Cat. #PD 178; L-Cysteine, Cat: C7352, Sigma; LipofectAmine, Invitrogen, Cat #50470; Maackia amurensis agglutinin 1 mg/ml, cat: L8025, Lot: 036k4075 Sigma; MAA-FITC, 2 mg in 2 ml buffer, EY, Cat. # F-7801-2; Maleimide, cat: 129585, Lot: S56783-278 Sigma; Na2PO4.



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    a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.
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    Image Search Results


    a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Journal: bioRxiv

    Article Title: Mouse behavioral genomics identifies Creld1 as a gatekeeper of somatosensation

    doi: 10.64898/2026.03.30.715210

    Figure Lengend Snippet: a , Schematic diagram of the CRISPR/Cas9-mediated knockout of target genes expressed in DRG neurons via intraventricular injection of the AAV9 virus carrying the construct containing the sgRNA against the target gene and EGFP for indication of infection efficiency. 6-8 weeks post injection, the mice were subjected to experimental tests. b , Representative single-cell Ca 2+ imaging of DRG neurons derived from control sgRNA- or sgTrpv1-targeted mice in response to 10 μM capsaicin and 50 mM KCl. Individual neurons are color-coded. c , Scatterplot of the percentage of cultured GFP + DRG neurons showing capsaicin-induced Ca 2+ response. d , Representative traces of mechanically evoked whole-cell currents of rapidly inactivating (RA), intermediate inactivating (IA), and slowly inactivating (SA) kinetics recorded from cultured control DRG neurons. e , Proportion of control sgRNA- and sgPiezo2-targted GFP + DRG neurons showing the indicated responding properties. f , The peak current density of tetradotoxin (TTX)-sensitive Na v current of DRG neurons derived from the control sgRNA-and sgNa v 1.7-targeted mice. g , Representative trace of evoked action potential (APs) of the control sgRNA- and sgNa v 1.7-targeted DRG neurons. h , Firing frequency of the control sgRNA- and sgNa v 1.7-targeted DRG neurons in response to the injected currents. i , Rheobase of sgNa v 1.7-targeted DRG neurons compared with the control sgRNA-targeted neurons. j , The percentage of paw withdrawals in response to the indicated series of Von Frey filament stimulation. k , Scatterplot of the percentage of paw withdrawal in response to the pinprick test. l , Scatterplot of the threshold in response to the Randall-Selitto test. m , Scatterplot of the paw withdrawal latency in response to the hot plate test. n , Scatterplot of the paw withdrawal latency in response to the cold plate test. o-q , Scatterplot of the scratch numbers within 30 minutes in mice injected with histamine ( o ), chloroquine ( p ) or compound 48/80 ( q ). Data are presented as means ± SEM; sample sizes are indicated. Statistical significance is determined by Unpaired Student’s t test for c , f , i . Two-way ANOVA with Bonferroni’s multiple comparisons test for j . One-way ANOVA with Bonferroni’s multiple comparisons test for k - q , * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

    Article Snippet: In brief, cells were washed with the Ca 2+ imaging buffer (1 x HBSS buffer with 1.3 mM Ca 2+ , 10 mM HEPES, pH 7.2) and then loaded with the Ca 2+ imaging buffer containing 2.5 mM Ca 2+ indicator dye Fura-2, AM (ThermoFisher scientific, F1225) and 0.05% Pluronic F-127 (Beyotime, ST501).

    Techniques: CRISPR, Knock-Out, Injection, Virus, Construct, Infection, Single Cell, Imaging, Derivative Assay, Control, Cell Culture, Randall–Selitto Test, Hot Plate Test