Review





Similar Products

odyssey clx  (LI-COR)
98
LI-COR odyssey clx
Odyssey Clx, supplied by LI-COR, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/Odyssey+CLx+Image+Studio+Analysis+Software+-+In-Cell+Western+Add-On/10__1007_slash_s10565___026___10287___9-234-6-8
Average 98 stars, based on 1 article reviews
odyssey clx - by Bioz Stars, 2026-10
98/100 stars
  Buy from Supplier
zeiss zen software  (Carl Zeiss)
95
Carl Zeiss zeiss zen software
Zeiss Zen Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/ZEN+Module+Image+Analysis/10__3390_slash_pharmaceutics18101246-147-9-9
Average 95 stars, based on 1 article reviews
zeiss zen software - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
imaris image analysis software  (Oxford Instruments)
99
Oxford Instruments imaris image analysis software
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Imaris Image Analysis Software, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/Imaris/pmc13620297-332-6-6
Average 99 stars, based on 1 article reviews
imaris image analysis software - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
image analysis software zen 3 13  (Carl Zeiss)
95
Carl Zeiss image analysis software zen 3 13
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Image Analysis Software Zen 3 13, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/ZEN+Module+Image+Analysis/pm42767265-63-12-17
Average 95 stars, based on 1 article reviews
image analysis software zen 3 13 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier
leica ai aivia platform  (Danaher Inc)
96
Danaher Inc leica ai aivia platform
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Leica Ai Aivia Platform, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/Aivia+AI+Image+Analysis+Software/pmc13637730-277-4-4
Average 96 stars, based on 1 article reviews
leica ai aivia platform - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
microscopy image analysis software 10 2  (Oxford Instruments)
99
Oxford Instruments microscopy image analysis software 10 2
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Microscopy Image Analysis Software 10 2, supplied by Oxford Instruments, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/Imaris/pmc13641440-70-5-4
Average 99 stars, based on 1 article reviews
microscopy image analysis software 10 2 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
digital pathological image analysis software  (Servicebio Inc)
86
Servicebio Inc digital pathological image analysis software
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Digital Pathological Image Analysis Software, supplied by Servicebio Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/analysis+digital+image+pathology+software/pmc13251493-65-9-6
Average 86 stars, based on 1 article reviews
digital pathological image analysis software - by Bioz Stars, 2026-10
86/100 stars
  Buy from Supplier
computer running image analysis software  (Carl Zeiss)
96
Carl Zeiss computer running image analysis software
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Computer Running Image Analysis Software, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/ZEN+lite+%2F+starter+Module+Image+Analysis/10__1038_slash_s41598___026___63111___z-105-32-39
Average 96 stars, based on 1 article reviews
computer running image analysis software - by Bioz Stars, 2026-10
96/100 stars
  Buy from Supplier
image lab analysis software version 5 2 1  (Bio-Rad)
99
Bio-Rad image lab analysis software version 5 2 1
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Image Lab Analysis Software Version 5 2 1, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/Image+Lab+Software+for+Mac+Version+5%2E2%2E1/10__1038_slash_s41598___026___63336___y-109-5-11
Average 99 stars, based on 1 article reviews
image lab analysis software version 5 2 1 - by Bioz Stars, 2026-10
99/100 stars
  Buy from Supplier
zeiss zen software v3 1  (Carl Zeiss)
95
Carl Zeiss zeiss zen software v3 1
Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using <t>Image</t> J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by <t>Imaris</t> image <t>analysis</t> <t>software</t> ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.
Zeiss Zen Software V3 1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software/ZEN+Module+Image+Analysis/pmc13651046-56-17-17
Average 95 stars, based on 1 article reviews
zeiss zen software v3 1 - by Bioz Stars, 2026-10
95/100 stars
  Buy from Supplier

Image Search Results


Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using Image J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by Imaris image analysis software ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.

Journal: Aging Cell

Article Title: uPAR ‐Targeting T Cell Engager Exerts Senolytic Effects in Mice and Non‐Human Primates With Serum Aminotransferase Activity as a Safety Monitor

doi: 10.1111/acel.70742

Figure Lengend Snippet: Physical attributes of naturally aged mice were enhanced following mGFD‐CD3 treatment. (A) Maximal walking speed, treadmill endurance, hanging endurance, and grip strength of naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded at 6 months after the first infusion ( n = 5 mice per condition). (B) H&E staining of gastrocnemius collected from mice treated with mCD3 or mGFD‐CD3, sampled 6 months after the first infusion. Scale bar, 50 μm. Relative cell areas in the gastrocnemius were quantified using Image J ( n = 5 mice per condition). (C) Representative micro‐CT images of the distal femurs of the mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion. The relative bone volume fraction (BV/TV) of cancellous bone in the distal femur was quantified for each sample and analyzed by Imaris image analysis software ( n = 5 mice per condition). (D) The percentages of total lean mass and total adipose mass and the adipose mass/lean mass ratio of naturally aged mice treated with mCD3 or mGFD‐CD3 6 months after the first infusion were recorded using InAlyzer software ( n = 5 mice per condition). (E) (left) Micro‐CT images of SAT and bone of the naturally aged mice treated with mCD3 or mGFD‐CD3 were recorded 6 months after the first infusion. Green, SAT; red, bone. (right) Quantification of the adipose tissue of mice based on the micro‐CT images ( left ). Data were normalized to mouse body weight ( n = 5 mice per condition). (F) Basal glucose levels after 12 h of starvation in mice treated with mCD3 or mGFD‐CD3 at 6 months after the first infusion ( n = 5 mice per condition). (G) ( upper ) Levels of glucose before (0 min) and after the intraperitoneal administration of glucose (2 g per kg body weight) (samples as per in (f)). ( lower ) Area under the curve (AUC) for the results from (g) ( n = 5 mice per condition). The mouse experiments are representative results from two independent experiments. Data are presented as mean ± SD and analyzed by one‐way ANOVA followed by Tukey's multiple comparisons test (A–G). * p ≤ 0.05; ** p ≤ 0.01.

Article Snippet: 3D image analysis was performed with Imaris image analysis software (version 10.1.0).

Techniques: Staining, Micro-CT, Analysis, Software, Tissue