tl100 software (Fotodyne Inc)
90
Structured Review
Fotodyne Inc
tl100 software
Tl100 Software, supplied by Fotodyne Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software+total+lab/total+lab+quant+analysis+software/pmc04305183-243-22-16
Average 90 stars, based on 1 article reviews
Tl100 Software, supplied by Fotodyne Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/image+analysis+software+total+lab/total+lab+quant+analysis+software/pmc04305183-243-22-16
Average 90 stars, based on 1 article reviews
tl100 software - by Bioz Stars,
2026-10
90/100 stars
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Western Blot:Article Title: RhoA GTPase-Induced Ocular Hypertension in a Rodent Model Is Associated with Increased Fibrogenic Activity in the Trabecular Meshwork Article Snippet: The cell lysates derived from HTM cells that expressed RhoAV14/GFP or GFP alone were immunoblotted for total RhoA, GFP, pMLC, phosphorylated-myosin phosphatase targeting subunit (pMYTP1), α-SMA, and β-actin with the use of the respective polyclonal (RhoA from Santa Cruz Biotechnology, Inc., Santa Cruz, CA; GFP from BD Biosciences, San Jose, CA; pMLC from Cell Signaling Technologies; and pMYPT1 from Millipore, Billerica, MA) or monoclonal (α-SMA and β-actin from Sigma-Aldrich) primary antibodies in conjunction with relevant secondary antibodies as described earlier. .. 21,30 Immunoblots were developed by enhanced chemiluminescence and were scanned densitometrically with a Gel Doc scanner (FOTODYNE Inc., Hartland, WI) equipped with Article Title: L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins Article Snippet: .. Immunoblots were then developed using enhanced chemiluminescence ECL prime reagents (Amersham Biosciences, Buckinghamshire, UK; C. No. RPN2232), and scanned using a FOTODYNE Gel Doc scanner equipped with Article Title: Lymphatic diamine oxidase secretion stimulated by fat absorption is linked with histamine release Article Snippet: .. After rinsing with TBS with 0.1% Tween 20, the membranes were further incubated with peroxidase-conjugated anti-goat secondary antibody at 1:2,000 for 1 h and then developed with ECL Western blot detection kit (GE Healthcare) according to the manufacturer's protocol and were quantified with the Total Software:Article Title: RhoA GTPase-Induced Ocular Hypertension in a Rodent Model Is Associated with Increased Fibrogenic Activity in the Trabecular Meshwork Article Snippet: The cell lysates derived from HTM cells that expressed RhoAV14/GFP or GFP alone were immunoblotted for total RhoA, GFP, pMLC, phosphorylated-myosin phosphatase targeting subunit (pMYTP1), α-SMA, and β-actin with the use of the respective polyclonal (RhoA from Santa Cruz Biotechnology, Inc., Santa Cruz, CA; GFP from BD Biosciences, San Jose, CA; pMLC from Cell Signaling Technologies; and pMYPT1 from Millipore, Billerica, MA) or monoclonal (α-SMA and β-actin from Sigma-Aldrich) primary antibodies in conjunction with relevant secondary antibodies as described earlier. .. 21,30 Immunoblots were developed by enhanced chemiluminescence and were scanned densitometrically with a Gel Doc scanner (FOTODYNE Inc., Hartland, WI) equipped with Article Title: L-Type Calcium Channels Play a Critical Role in Maintaining Lens Transparency by Regulating Phosphorylation of Aquaporin-0 and Myosin Light Chain and Expression of Connexins Article Snippet: .. Immunoblots were then developed using enhanced chemiluminescence ECL prime reagents (Amersham Biosciences, Buckinghamshire, UK; C. No. RPN2232), and scanned using a FOTODYNE Gel Doc scanner equipped with Article Title: Lymphatic diamine oxidase secretion stimulated by fat absorption is linked with histamine release Article Snippet: .. After rinsing with TBS with 0.1% Tween 20, the membranes were further incubated with peroxidase-conjugated anti-goat secondary antibody at 1:2,000 for 1 h and then developed with ECL Western blot detection kit (GE Healthcare) according to the manufacturer's protocol and were quantified with the Total Article Title: Angiotensin II directly impairs adipogenic differentiation of human preadipose cells. Article Snippet: Angiotensin II reduces adipogenic differentiation of preadipose cells present in the stroma-vascular fraction of human adipose tissue, which also includes several cell types.. Because of the ability of non-adipose lineage cells in the stroma-vascular fraction to respond to angiotensin II, it is not possible to unequivocally ascribe the anti-adipogenic response to a direct effect of this hormone on preadipose cells.. Therefore, we used the human Simpson–Golabi–Behmel syndrome (SGBS) preadipocyte cell strain to investigate the consequences of angiotensin II treatment on adipogenic differentiation under serum-free conditions, by assessing expression of typical adipocyte markers perilipin and fatty acid-binding protein 4 (FABP4), at the transcript and protein level. Article Title: Glucagon-like peptide-2 mobilization of intestinal lipid does not require canonical enterocyte chylomicron synthetic machinery. Article Snippet: Background & aims: Dietary triglycerides (TG) retained in the intestine after a meal can be mobilized many hours later by glucagon-like peptide-2 (GLP-2) in humans and animal models, despite the well-documented absence of expression of the GLP-2 receptor on enterocytes.. In this study, we examined the site of GLP-2 action to mobilize intestinal lipids and enhance chylomicron production.. Methods: In mesenteric lymph duct-cannulated rats, we assessed GLP-2-stimulated lymph flow rate, TG concentration, TG output, and apoB48 abundance 5 h after an intraduodenal lipid bolus, in the presence of a validated GLP-2 antagonist or vehicle. Article Title: Apolipoprotein A-V is present in bile and its secretion increases with lipid absorption in Sprague-Dawley rats Article Snippet: After a rinsing with TBS with 0.1% Tween 20, membranes were incubated with peroxidase-conjugated anti-goat secondary antibody at 1:10,000 for 30 min and developed with Immobilon Western Chemiluminescent horseradish peroxidase substrate (Millipore, Billerica, MA). .. Protein quantification was performed by densitometric analysis of films using Total Article Title: Apolipoprotein A-V deficiency enhances chylomicron production in lymph fistula mice Article Snippet: After being rinsed with TBS plus 0.1% Tween 20, membranes were incubated with peroxidase-conjugated anti-goat antibody at 1:10,000 for 30 min, and signal was developed with Immobilon Western Chemiluminescent HRP substrate (EMD Millipore, Billerica, MA). .. Protein bands were quantified by densitometric analysis using Total Incubation:Article Title: Lymphatic diamine oxidase secretion stimulated by fat absorption is linked with histamine release Article Snippet: .. After rinsing with TBS with 0.1% Tween 20, the membranes were further incubated with peroxidase-conjugated anti-goat secondary antibody at 1:2,000 for 1 h and then developed with ECL Western blot detection kit (GE Healthcare) according to the manufacturer's protocol and were quantified with the Total Control:Article Title: Angiotensin II directly impairs adipogenic differentiation of human preadipose cells. Article Snippet: Angiotensin II reduces adipogenic differentiation of preadipose cells present in the stroma-vascular fraction of human adipose tissue, which also includes several cell types.. Because of the ability of non-adipose lineage cells in the stroma-vascular fraction to respond to angiotensin II, it is not possible to unequivocally ascribe the anti-adipogenic response to a direct effect of this hormone on preadipose cells.. Therefore, we used the human Simpson–Golabi–Behmel syndrome (SGBS) preadipocyte cell strain to investigate the consequences of angiotensin II treatment on adipogenic differentiation under serum-free conditions, by assessing expression of typical adipocyte markers perilipin and fatty acid-binding protein 4 (FABP4), at the transcript and protein level. |