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data analysis software  (Bio-Rad)


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  • 99

    Structured Review

    Bio-Rad data analysis software
    Data Analysis Software, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 24633 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/image+analysis+software+data/ChemiDoc+MP+Imaging+System/pmc12852233-109-14-21
    Average 99 stars, based on 24633 article reviews
    data analysis software - by Bioz Stars, 2026-11
    99/100 stars

    Images

    Related Articles

    Imaging:

    Article Title: Spatial decoupling of recognition and signaling in aptamer switches via tri-strand intermolecular competition.
    Article Snippet: .. The gel was run first at 50 V for 10 min and then at 100 V for 105 min. Gels were then prestain scanned using the Bio- Rad ChemiDoc MP Imaging System, then stained with 1× SYBR Safe for 20 to 30 min, and poststain scanned. ..

    Article Title: Ca 2+ -dependent pathways controlled by Piezo1 activity in human erythroid progenitor cell line: focus on KCa3.1 channels, ANO6 lipid scramblases and pannexins-1.
    Article Snippet: The membrane was blocked with 5% nonfat milk in 1x PBS-T solution for 1 hour, incubated with primary anti-Panx1 polyclonal antibodies (Proteintech, USA, Cat No 12595-1-AP) at a dilution of 1:1000 at +4 °C overnight, and then with a goat anti-rabbit IgG-peroxidase secondary antibody (Sigma‒Aldrich, USA, Cat. No A0545) at a 1:100000 dilution for 1 hour. .. Bands were visualized via an enhanced chemiluminescence (ECL) substrate (Super Signal West Femto Maximum Sensitivity Substrate, Thermo Fisher Scientific, USA) on a gel/blot imaging system (ChemiDoc, Bio-Rad Laboratories, USA). .. Statistics GraphPad Prism 8.0 (GraphPad Software, USA) and Microsoft Excel (MS Corporation, USA) were used for statistical analysis.

    Article Title: Targeting PI3K/AKT/mTOR Pathway Exerts Inhibitory Effects on Endothelial Cells from Generalized Lymphatic Anomaly.
    Article Snippet: IRDye 680LT goat anti-mouse IgG (#925-68020) and IRDye 800CW goat anti-rabbit IgG (#925-32211) from LI-COR Biotechnology (Lincoln, NE, USA) were used as secondary species-specific antibodies at a dilution factor of 1:5000. .. Signals were detected using the ChemiDocTM MP Imaging System (Bio-Rad Laboratories, Hercules, CA, USA) and quantified with ImageJ v.1.54p software (https://imagej.net/ij/; accessed on 5 October 2026). ..

    Article Title: An in situ forming immunomodulatory hydrogel reinvigorates exhausted CD8 + T cells via preserving their mitochondrial fitness
    Article Snippet: .. After final washes, signals were detected using enhanced chemiluminescence and imaged with the ChemiDoc Touch Imaging System (Bio-Rad, USA). ..

    Article Title: 5-Hydroxytryptamine 6 Receptor Antagonism Ameliorates Depression-Like Behaviors in Temporal Lobe Epilepsy Rats with Potential Involvement of the MEF2C/NF-κB Pathway.
    Article Snippet: The elevated plus-maze (Model EPM-100) and Morris water maze (Model MWM100) were provided by RWD Life Science Co., Ltd. (China). .. ChemiDoc XRS+ gel imaging system was purchased from Bio-Rad (USA). .. cryomicrotome was bought from Leica (Model EMUC7FC7, Germany).

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: The next day, membranes were washed three times with PBS and incubated with goat anti-rabbit HRP-conjugated immunoglobulin G (IgG) (Cell Signaling Technology, catalog no. 7074, RRID: AB_2099233) or horse anti-mouse IgG HRP-conjugated (Cell Signaling Technology, catalog no. 7076, RRID: AB_330924) for 1 hour at room temperature. .. Protein signals were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, catalog no. 34094) and visualized with the Chemidoc MP Imaging System (Bio-Rad). .. The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution.

    Article Title: ERα–PADI4–CXCL8 Axis Drives MDSC‐Mediated Immune Evasion in Anaplastic Thyroid Carcinoma
    Article Snippet: After three washes with TBST, membranes were incubated with horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG secondary antibody (CST #7074, 1:5000) for 1 h at room temperature. .. Protein bands were visualized using SuperSignal West Pico Chemiluminescent Substrate (Thermo Fisher) on a ChemiDoc XRS+ imaging system (Bio‐Rad). .. Band intensities were quantified using ImageJ software (NIH, v1.53t) and normalized to GAPDH.

    Staining:

    Article Title: Spatial decoupling of recognition and signaling in aptamer switches via tri-strand intermolecular competition.
    Article Snippet: .. The gel was run first at 50 V for 10 min and then at 100 V for 105 min. Gels were then prestain scanned using the Bio- Rad ChemiDoc MP Imaging System, then stained with 1× SYBR Safe for 20 to 30 min, and poststain scanned. ..

    Sensitivity:

    Article Title: Ca 2+ -dependent pathways controlled by Piezo1 activity in human erythroid progenitor cell line: focus on KCa3.1 channels, ANO6 lipid scramblases and pannexins-1.
    Article Snippet: The membrane was blocked with 5% nonfat milk in 1x PBS-T solution for 1 hour, incubated with primary anti-Panx1 polyclonal antibodies (Proteintech, USA, Cat No 12595-1-AP) at a dilution of 1:1000 at +4 °C overnight, and then with a goat anti-rabbit IgG-peroxidase secondary antibody (Sigma‒Aldrich, USA, Cat. No A0545) at a 1:100000 dilution for 1 hour. .. Bands were visualized via an enhanced chemiluminescence (ECL) substrate (Super Signal West Femto Maximum Sensitivity Substrate, Thermo Fisher Scientific, USA) on a gel/blot imaging system (ChemiDoc, Bio-Rad Laboratories, USA). .. Statistics GraphPad Prism 8.0 (GraphPad Software, USA) and Microsoft Excel (MS Corporation, USA) were used for statistical analysis.

    Article Title: CASTOR1 functional inactivation drives KRAS -mutant NSCLC and therapy resistance by a noncanonical mechanism of tumor suppression
    Article Snippet: The next day, membranes were washed three times with PBS and incubated with goat anti-rabbit HRP-conjugated immunoglobulin G (IgG) (Cell Signaling Technology, catalog no. 7074, RRID: AB_2099233) or horse anti-mouse IgG HRP-conjugated (Cell Signaling Technology, catalog no. 7076, RRID: AB_330924) for 1 hour at room temperature. .. Protein signals were detected using SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific, catalog no. 34094) and visualized with the Chemidoc MP Imaging System (Bio-Rad). .. The primary antibodies used in the analysis included the following: anti-CASTOR1 (Abclonal, catalog no. A2071C), anti-AKT (Cell Signaling Technology, catalog no. 4691, RRID:AB_915783), anti–p-AKT–S473 (Cell Signaling Technology, catalog no. 4060), anti–p-AKT–T308 (Cell Signaling Technology, catalog no. 4056, RRID:AB_331163), anti-S6K (Cell Signaling Technology, catalog no. 97596, RRID:AB_2800283), anti-4EBP1 (Cell Signaling Technology, catalog no. 9644, RRID:AB_2097841), anti–p-4EBP1 (Cell Signaling Technology, catalog no. 2855), anti-S6 (Cell Signaling Technology, catalog no. 2217, RRID:AB_331355), anti–p-S6 (Cell Signaling Technology, catalog no. 2211), anti–p-ERK (Cell Signaling Technology, catalog no. 4370), anti-RAS (Cell Signaling Technology, catalog no. 91054), anti-KRAS G12D (Cell Signaling Technology, catalog no. 14429, RRID:AB_2728748), and anti-GAPDH (Cell Signaling Technology, catalog no. 5174, RRID:AB_10622025), all of which were used at 1:1000 dilution.

    Software:

    Article Title: Targeting PI3K/AKT/mTOR Pathway Exerts Inhibitory Effects on Endothelial Cells from Generalized Lymphatic Anomaly.
    Article Snippet: IRDye 680LT goat anti-mouse IgG (#925-68020) and IRDye 800CW goat anti-rabbit IgG (#925-32211) from LI-COR Biotechnology (Lincoln, NE, USA) were used as secondary species-specific antibodies at a dilution factor of 1:5000. .. Signals were detected using the ChemiDocTM MP Imaging System (Bio-Rad Laboratories, Hercules, CA, USA) and quantified with ImageJ v.1.54p software (https://imagej.net/ij/; accessed on 5 October 2026). ..

    other:

    Article Title: Modified tumor uptake and biodistribution of nanoparticles coated with small extracellular vesicle membranes derived from distinct tumor cell lines
    Article Snippet: Membranes were then washed with tris buffered saline containing 0.1% tween-20 followed by 2 h of hybridization with 1:5000 dilution of horseradish peroxidase-conjugated goat anti-rat IgG (NB7115, Novus Biological) or goat anti-mouse IgG (170–6516, BioRad).



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